STAR Protocols
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2023
·
IF 0
Jinhao Hu,Xinqi Zhao,Guoying Bai
Summary The development of biocompatible ice-controlling materials for non-vitreous cryopreservation of cells is of great importance to the field of biomedicine. Here, we present a protocol to use fulvic acid (FA) for efficient non-vitreous cryopreservation of red blood cells (RBCs) that both promotes the melting of ice crystals and retards their growth/recrystallization. We describe steps for FA fractionation and performing tests for ice recrystallization and ice freezing/thawing. We then detail the freezing/thawing of RBCs, recovering RBCs, and testing their viability. For complete details on the use and execution of this protocol, please refer to Bai et al. (2022). 1
研究领域:
生物医学工程
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低温生物学
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血液学
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DOI
PubMed
Materials Today Bio
·
2025
·
IF 10.2
Qi Feng,Jianping Wang,Dengyao Yi,Suhuan Li,Yue Cheng,Zhongrong Chen,Gang Zhao
Cryopreservation and transplantation of ovarian tissue have emerged as promising techniques for preserving fertility in prepubescent girls and cancer patients who cannot postpone treatment. However, this technology faces several challenges. In particular, conventional methods often fail to mitigate serious oxidative stress issues and cytotoxic damage caused by cryoprotectants, which can affect transplant outcomes. To overcome this bottleneck, a new strategy was proposed here, a new cryoprotectant formula (12% ethylene glycol+12% propylene glycol +0.5 M sucrose+75 μM epigallocatechin gallate) was developed and combined with hydrogel encapsulation to optimize ovarian cryopreservation. This approach avoids the utilization of DMSO and reduces the concentration of penetrating cryoprotectant by 20%. The findings indicate that this strategy significantly reduced oxidative damage, fibrosis degree, and tissue apoptosis during cryopreservation and enhanced the quality of cryopreserved mouse ovaries. Furthermore, in vivo and in vitro tests assessing ovarian and oocyte quality further validate the safety and reliability of this research protocol. This study is anticipated to provide a new strategy for female fertility preservation through ovarian cryopreservation.
研究领域:
生殖医学
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生物技术
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产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
·
2024
·
IF 9.4
Yunqing Tian,Yiming Ma,Yuxiang Kang,Shu Tian,Qingsi Li,Lei Zhang,Jing Yang
Hypothermic preservation (HP) is highly desired for the maintenance of the viability of living cell specimens, e.g. rare cells in whole-blood samples or therapeutic cells, in an unfrozen state. However, the extension of the viable preservation time is a challenge because of the multiple injuries suffered by hypothermically preserved cells. Here, based on a dynamic bond crosslinked zwitterionic hydrogel, we established a sensing preservation system that could monitor the levels of reactive oxygen species (ROS) via real-time electronic signals and intelligent control of antioxidant addition, to completely prevent an excess of ROS in the whole-cell specimen. Furthermore, the hydrogel-based system can counter the extracellular-matrix-loss-induced anoikis of living cells. Based on the design aimed at affording protection against two primary HP injuries (i.e. ROS overproduction and anoikis) to cells, this system extended the preservation time of cell specimens under refrigerated conditions to 24 days. After preservation, the use of a mild cell retrieval process guaranteed the activity of the preserved living cells. This work not only possesses the potential to facilitate intelligent cell-based clinical applications, but also paves the way for the preparation of living materials that can host programmed cells with long-term survival. Statement of significance An intelligent system based on a zwitterionic sensing hydrogel is established, which can afford ultra-long hypothermic cell-preservation times of up to 24 days. The system enables the real-time monitoring of ROS overproduction and intelligent antioxidant addition, because of the merging of the smart hydrogel with a computer intelligent detection and control system. Furthermore, the automatic addition of an antioxidant according to the ROS-signal changes produced by the ZBA hydrogel effectively prevented HP lesions, including ROS over-production and ECM loss, in the preserved living cells. Subsequently, the system could also be gently dissociated, to retrieve the preserved cells. This work provides a solution for the real-time monitoring and long-term HP of living specimens, which holds the promise of benefiting cell-based medicine and the development of genetically programmed cell-based living materials.
研究领域:
生物技术
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细胞生物学
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材料科学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Yu Wang,Qianzhan Yang,Huadong Zhang,Zhenghong Wang,Ailin Wu,Yun Luo,Qianru Zhou
Background The ongoing infusion of pharmaceutical and personal care products (PPCPs) into ecosystems sustains a perpetual life cycle and leads to multi-generational exposures. Limited understanding of their environmental impact and their intrinsic ability to induce physiological effect in humans, even at low doses, pose great risks to human health. Few scholarly works have conducted systematic research into the occurrence of PPCPs within potable water systems. Concurrently, the associated monitoring techniques have not been comprehensively examined with regards to the specific nature of drinking water, namely whether the significant presence of disinfectants may influence the detection of PPCPs. Results A modified approach in terms of detailed investigation of sample preservation and optimization of an in-lab fabricated solid phase extraction (SPE) cartridge filled with DVB-VP and PS-DVB sorbent was proposed. Favorable methodological parameters were achieved, with correlation coefficients spanning from 0.9866 to 0.9998. The LODs of the PPCPs fluctuated from 0.001 to 2 μg L −1 , while the LOQs varied from 0.002 to 5 μg L −1 . The analysis of spiked samples disclosed a methodological precision of 2.31–9.86 % and a recovery of 52.4–119 %. We utilized the established method for analyzing 14 water samples of three categories (source water, finished water and tap water) from five centralized water supply plants. A total of 24 categories encompassing 72 PPCPs were detected, with the concentrations of PPCPs manifested a marked decrease from source water to finished water and finally to tap water. Significance Our research meticulously examined the enhancement and purification effects of widely used commercial SPE cartridges and suggested the use of in-lab fabricated SPE cartridges packed with DVB-VP and PS-DVB adsorbents. We also conducted a systematic evaluation of the need to incorporate ascorbic acid and sodium thiosulfate as preservatives for PPCP measurement, in consideration of the unique characteristics of drinking water matrices, specifically, the significant concentration levels of disinfectants. Furthermore, the proposed method was effectively employed to study the presence of PPCPs in source water, finished water, and tap water collected from centralized water supply plants.
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
Polymers
·
2019
·Vol. 11
·期 5
·786
·
IF 3.164
Xu Yan,Guo-Sai Liu,Jing Yang,Yi Pu,Shuo Chen,Hong-Wei He,Conger Wang,Yun-Ze Long,Shouxiang Jiang
Paper-based relics, which are an important part of cultural heritage worldwide, are at risk of imminent damage from various environmental sources. To protect them, the atmospheric pressure plasma polymerization of hexamethyldisiloxane (HMDSO) precursor has been explored on paper-based relics in situ. The macro and micro images taken during this process suggest that the in situ plasma treatment does not change the macro morphology and the micro structure of the treated paper-based relic samples. On the other hand, plasma treatment causes the polymerization of the HMDSO which then produces nanoparticles deposited onto the paper-based relics. These nanoparticles provide good waterproof properties with large static water contact angles and smaller rolling angles, which protect the paper-based relics from water penetration. Moreover, since the nanoparticles are deposited onto the fibers, waterproof fastness is ensured. Also, the examined mechanical properties of the treated and untreated paper-based relics indicate that the atmospheric pressure plasma treatment does not affect the strength of the paper very much. The results in this study show that atmospheric pressure plasma treatment with the use of HMDSO precursor is a good method to preserve paper-based relics.Keywords:plasma treatment;paper-based relics;waterproofing;preservation
研究领域:
文物保护
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材料科学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-11
·
IF 3.8
Liu Hao,Xiong Zhengye,Chen Qingxiang,Wang Luyan,Wang Chunxi
To evaluate the effect of irradiation on the preservation of potatoes, fresh potatoes were selected as the irradiation objects, and irradiated with 60 Co- γ radiation source for 0, 100, 200, 500 and 1000 Gy, respectively. During the irradiation, the well-packaged Y 1.79 Bi 0.01 Eu 0.2 MgTiO 6 novel thermoluminescence dosimeter material was placed together with the potatoes at the same position. Then, the potatoes were stored in the same temperature and humidity environment, and the quality changes of the potatoes were observed. The Y 1.79 Bi 0.01 Eu 0.2 MgTiO 6 material had good performance indicators, and was used to measure the irradiation dose of the potatoes. The experiment showed that irradiation could appropriately extend the storage time of potatoes, and gamma irradiation of about 1000 Gy could achieve the best preservation effect. The main pathogenic fungi that cause dry rot of potatoes were Fusarium solani and Fusarium oxysporum , and the appropriate dose of 60 Co- γ irradiation could effectively inhibit the spread and growth of these fungi.
研究领域:
食品科学
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植物病理学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2025
·
IF 3.1
Yu-Qing Zhu,Jing-Wen Liu,Jing Zhou,Cheng-Ying Wu,Fang Long,Jin-Di Xu,Shan-Shan Zhou,Qian Mao,Ming Kong,Song-Lin Li,Hong Shen
Atractylodis Macrocephalae Rhizoma (AMR) is one of the commonly used medicinal and edible herbs in China. To prevent infestation of moths and mildew, chemical-fumigation is applied during post-harvest processing, which would produce harmful substances. Ultralow oxygen (ULO) storage, as a potential controlled atmosphere preservation method for Traditional Chinese Medicines (TCMs), has no residue of chemical hazards, but its impact on chemical composition of TCMs is unclear. Here, to evaluate the impact of ULO preservation on the quality of AMR, a UPLC-QTOF-MS/MS-based non-targeted metabolomics approach was employed to screen out the differential components after AMR had been stored under ULO conditions for 18 months. The differential components were identified, and further semi-quantified with peak areas. Additionally, the differential components with known activity were quantified using HPLC-DAD and GC-TQ-MS/MS analysis. The molecular weight distribution and monosaccharide composition of polysaccharides were analyzed using HPGPC-ELSD and HPLC-DAD to assess the impact of ULO on the carbohydrates. As it showed, 20 differential components of AMR under ULO were screened and identified when compared to normal atmosphere (NA) with 21 % oxygen concentration. As storage duration increased, the levels of atractylenolides I, II, and III in both ULO and NA storage conditions increased, with significant differences in their total content (16.06 mg/g in ULO vs. 47.57 mg/g in NA). Confirmatory experiments revealed that atractylone is the precursor compound of atractylenolides and the total content of atractylenolides I, II, and III produced from atractylone in ULO storage was only 62.12 % of that in NA at 18th month. The total sugar content of AMR polysaccharides in ULO storage was 107.28 % of that in NA and the molar ratios of monosaccharides were also relatively constant. All the results indicated that ULO preservation slowed down the chemical transformation of AMR, including small molecules and polysaccharides. Therefore, the quality of AMR stored in ULO is more stable and it is proposed to be an efficient preservation method for AMR.
研究领域:
中药学
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分析化学
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植物化学
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产品关联
DOI
PubMed
高影响因子
ACS Synthetic Biology
·
2023
·
IF 4.7
Peng Shen,Xiaojun Qu,Qinyu Ge,Ting Huang,Qingjiang Sun,Zuhong Lu
DNA is an attractive medium for long-term data storage because of its density, ease of copying, sustainability, and longevity. Recent advances have focused on the development of new encoding algorithms, automation, and sequencing technologies. Despite progress in these subareas, the most challenging hurdle in the deployment of DNA storage remains the reliability of preservation and the repeatability of reading. Herein, we report the construction of a magnetic bead spherical nucleic acid (MB-SNA) composite microstructure and its use as a cost-effective platform for reliable DNA preservation and repeated reading. MB-SNA has an inner core of silica@γ-Fe2O3@silica microbeads and an outer spherical shell of double-stranded DNA (dsDNA) with a density as high as 34 pmol/cm2. For MB-SNA, each strand of dsDNA stored a piece of data, and the high-density packing of dsDNA achieved high-capacity storage. MB-SNA was advantageous in terms of reliable preservation over free DNA. By accelerated aging tests, the data of MB-SNA is demonstrated to be readable after 0.23 million years of preservation at −18 °C and 50% relative humidity. Moreover, MB-SNA facilitated repeated reading by facile PCR-magnetic separation. After 10 cycles of PCR access, the retention rate of dsDNA for MB-SNA is demonstrated to be as high as 93%, and the accuracy of sequencing is more than 98%. In addition, MB-SNA makes cost-effective DNA storage feasible. By serial dilution, the physical limit for MB-SNA to achieve accurate reading is probed to be as low as two microstructures.
研究领域:
生物化学
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分子生物学
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数据科学
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产品关联
DOI
PubMed
高影响因子
Foods
·
2023
·Vol. 12
·期 12
·2275
·
IF 5.2
Nur Julizan,Safri Ishmayana,Achmad Zainuddin,Pham Van Hung,Dikdik Kurnia
Food preservation is one of the strategies taken to maintain the level of public health. Oxidation activity and microbial contamination are the primary causes of food spoilage. For health reasons, people prefer natural preservatives over synthetic ones.Syzygnium polyanthumis widely spread throughout Asia and is utilized as a spice by the community.S. polyanthumhas been found to be rich in phenols, hydroquinones, tannins, and flavonoids, which are potential antioxidants and antimicrobial agents. Consequently,S. polyanthumpresents a tremendous opportunity as a natural preservative. This paper reviews recent articles aboutS. polyanthumdating back to the year 2000. This review summarizes the findings of natural compounds presented inS. polyanthumand their functional properties as antioxidants, antimicrobial agents, and natural preservatives in various types of food.Keywords:Syzygnium polyanthum;antioxidant;antimicrobials;natural preservative;food preservation
研究领域:
植物学
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食品科学
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药理学
✓ 已验证
DOI
PubMed
高影响因子
Food Science & Nutrition
·
2022
·Vol. 10
·期 9
·3098-3105
·
IF 3.553
Yongmin Wu,Yi Wu,Peng Han,Jiangqi Xu,Xiaobo Liang
The effects of edible coatings based on sodium alginate with ʻBaozhuʼ pear chitinase on the quality of cherry tomatoes during refrigerated storage were evaluated. Cherry tomatoes inoculated with Fusarium oxysporum were coated and stored up to 21 days. All coatings with the chitinase significantly reduced F . oxysporum proliferation on cherry tomatoes during storage and extended the shelf life of cherry tomatoes effectively ( p < .05). Results showed that alginate coatings with the chitinase could prevent weight loss, maintain firmness, and slow down the changes of titratable acidity and vitamin C ( p < .05) in a dose-dependent manner. However, no significant differences were observed between T3 (1% alginate/0.15% ʻBaozhuʼ pear chitinase/1% glycerin) and T4 (1% sodium alginate/0.3% ʻBaozhuʼ pear chitinase/1% glycerin) ( p > .05). Overall, alginate coating with 0.15% ʻBaozhuʼ pear chitinase could be a promising method to maintain the quality of cherry tomatoes.
研究领域:
食品科学
·
植物病理学
✓ 已验证
DOI
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 7
·6696
·
IF 5.6
Yuying Hu,Xiangjian Liu,Marlene Davis Ekpo,Jiangming Chen,Xiaoxiao Chen,Wenqian Zhang,Rui Zhao,Jingxian Xie,Yongju He,Songwen Tan
The cryopreservation of red blood cells (RBCs) holds great potential for ensuring timely blood transfusions and maintaining an adequate RBC inventory. The conventional cryoprotectants (CPAs) have a lot of limitations, and there is an obvious need for novel, efficient, and biocompatible CPAs. Here, it is shown for the first time that the addition of dimethylglycine (DMG) improved the thawed RBC recovery from 11.55 ± 1.40% to 72.15 ± 1.22%. We found that DMG could reduce the mechanical damage by inhibiting ice formation and recrystallization during cryopreservation. DMG can also scavenge reactive oxygen species (ROS) and maintain endogenous antioxidant enzyme activities to decrease oxidative damage during cryopreservation. Furthermore, the properties of thawed RBCs were found to be similar to the fresh RBCs in the control. Finally, the technique for order performance by similarity to ideal solution (TOPSIS) was used to compare the performance of glycerol (Gly), hydroxyethyl starch (HES), and DMG in cryopreservation, and DMG exhibited the best efficiency. This work confirms the use of DMG as a novel CPA for cryopreservation of RBCs and may promote clinical transfusion therapy.Keywords:cryopreservation;dimethylglycine;red blood cells;cryoprotectant
研究领域:
血液学
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生物技术
·
低温生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2022
·Vol. 13
·期 1
·1-16
·
IF 17.694
Tian Conghui,Shen Lingxiao,Gong Chenjia,Cao Yunxia,Shi Qinghua,Zhao Gang
Preantral follicles are often used as models for cryopreservation and in vitro culture due to their easy availability. As a promising approach for mammalian fertility preservation, vitrification of preantral follicles requires high concentrations of highly toxic penetrating cryoprotective agents (up to 6 M). Here, we accomplish low-concentration-penetrating cryoprotective agent (1.5 M) vitrification of mouse preantral follicles encapsulated in hydrogel by nanowarming. We find that compared with conventional water bath warming, the viability of preantral follicles is increased by 33%. Moreover, the cavity formation rate of preantral follicles after in vitro culture is comparable to the control group without vitrification. Furthermore, the percentage of MII oocytes developed from the vitrified follicles, and the birth rate of offspring following in vitro fertilization and embryo transfer are also similar to the control group. Our results provide a step towards nontoxic vitrification by utilizing the synergistic cryoprotection effect of microencapsulation and nanowarming.
研究领域:
生殖生物学
·
低温生物学
·
组织工程
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）