Biomolecules
·
2023
·Vol. 13
·期 3
·496
·
IF 5.5
Yingyuan Zhao,Zhaoxuan Wang,Shuxian Liu,Shiying Xie,Yinchun Xie,Ruifang Li,Hiroaki Oda
Astaxanthin is a carotenoid with excellent antioxidant activity. However, this small lipid-soluble molecule is insoluble in water and has low stability. Although this situation can be improved when astaxanthin is prepared as a nanosuspension, the aqueous form is still not as convenient and safe as the dry powder form for storage, transport, and use. The lyophilization process provides better protection for thermosensitive materials, but this leads to collapse and agglomeration between nanoparticles. To improve this situation, appropriate lyophilization protectants are needed to offer support between the nanoparticles, such as sugars, amino acids, and hydroxy alcohols. The purpose of this work is to screen lyophilization protectants by single-factor experiments and response surface optimization experiments and then explore the optimal ratio of compound lyophilization protectants, and finally, make excellent astaxanthin/BSA/chitosan nanosuspension (ABC-NPs) lyophilized powder. The work shows that the optimal ratio of the compounding lyophilization protectant is 0.46% oligomeric mannose, 0.44% maltose, and 0.05% sorbitol (w/v). The ABC-NPs lyophilized powder prepared under the above conditions had a re-soluble particle size of 472 nm, with a ratio of 1.32 to the particle size of the sample before lyophilization. The lyophilized powder was all in the form of a pink layer. The sample was fluffy and dissolved entirely within 10 s by shaking with water. Consequently, it is expected to solve the problem of inconvenient storage and transportation of aqueous drugs and to expand the application of nanomedicine powders and tablets.Keywords:lyophilization;astaxanthin nanoparticles;freeze-drying protectant
研究领域:
药学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
REACTIVE & FUNCTIONAL POLYMERS
·
2025
·
IF 5.1
Ke Shang,Qingjing Niu,Xiaohui Li,Kongying Zhu,Lixia Ren,Xiaoyan Yuan
Ice formation can cause a serious of cryoinjuries to cells, and development of biocompatible cryoprotectants for ice control is of great importance. Herein, ice-nucleating glycopolymers are synthesized from the alternating copolymer, poly(methyl vinyl ether- alt -maleic anhydride) (PMVEA), by modification with small saccharides via one-step esterification. The prepared water-soluble glycopolymers can trigger ice nucleation at −14 to −12 °C, significantly higher than deionized water, linear poly(vinyl alcohol), and poly(ethylene glycol) (PEG). The structural differences in monosaccharide, disaccharide and trisaccharide-grafted glycopolymers have positive impacts on the chemically induced ice nucleation and the ice crystal morphology, but all of them have weak activities to inhibit ice recrystallization, similar like PEG. Analyses of differential scanning calorimetry and low field nuclear magnetic resonance suggest that the PMVEA-based glycopolymers, which have an alternating structure of hydrophobic methoxyl and hydrophilic carboxyl or saccharide residues, could have strong interactions with water molecules, thus initiating ice nucleation. Particularly, incorporation of trehalose-modified PMVEA in alginate hydrogel can enhance cryosurvival of Lactobacillus rhamnosus after cryopreservation at −20 °C in comparison with cryostorage at −80 °C. This work provides a feasible approach for preparation of ice-nucleating macromolecules for ice control during cryopreservation of cells.
研究领域:
生物材料学
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低温生物学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2024
·
IF 8.5
Yejun Zhang,Yongzhen Wang,Hao Dong,Jiao Li,Jianan Sun,Xiangzhao Mao
Temperature fluctuations can negatively affect the quality of frozen shrimp. Research on novel cryoprotectants to replace traditional agents (phosphate, etc.) has become a hotspot. Our results indicated that L-Proline could reduce thawing losses, delay texture deterioration and improve the functional properties of myofibrillar proteins of shrimp. Thawing loss in the proline group (3.2 %) was significantly lower than that in the control (5.4 %) after 3 freeze-thaw cycles ( p < 0.05). Compared to Na 4 P 2 O 7 , proline had better permeability and greater ability to inhibit ice crystal growth and volume expansion. Through molecular simulations, we found that proline might inhibit ice crystal formation by forming glassy states with water. Hydrogen bonding between proline and water/ice played a major role, and only a small amount of proline was required to significantly reduce the ice crystal growth rate from 0.16 m/s to 0.06 m/s. Briefly, proline exhibited potential as a cryoprotectant for shrimp in temperature fluctuations.
研究领域:
食品科学
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分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF STORED PRODUCTS RESEARCH
·
2025
·
IF 2.8
Xiangfei Li,Menghan Wang,Meng Ding,Xinyi Pang,Jing Sun,Yingjian Lu
Freeze-drying is a common method for preserving bacteria cells, but it can cause cell damage in the process of protection. Therefore, it is necessary to explore a cryoprotectant to improve cell survival during the process. This research aimed to explore the optimal cryoprotectant formulation of L. acidophilus FMNS-10 and its protection mechanism. The optimal cryoprotectant consisted of 10.7 % skim milk, 3 % sodium L-glutamate and 10 % D-trehalose, resulting in a survival rate of 91.69 % for L. acidophilus FMNS-10 after freeze-drying ( p < 0.05). Under this condition, the integrity of cell wall and membrane of L. acidophilus FMNS-10 was close to that of the fresh strain, which could be attributed to preventing ice crystal formation. Cryoprotectants demonstrated a significant protective effect on enzymatic activities. In the cryoprotectant-free group, the activities of β-galactosidase, L-LDH, Na + /K + -ATPase and the concentration of Ca 2+ decreased by 36.19 %, 51.82 %, 89.40 %, and 41.90 %, respectively. However, the cryoprotectant group were only 7.10 %, 1.66 %, 7.41 %, and 4.10 % reductions compared to the cryoprotectant-free group ( p < 0.05). These results indicated that the optimal cryoprotectant improved the survival rate of L. acidophilus FMNS- 10 by inhibiting the loss of enzyme activity. In addition, the optimal cryoprotectant significantly mitigated the DNA damage induced by freeze-drying. The reduction in enzyme and DNA damage may be attributed to complex cryoprotectants maintaining the stability of their three-dimensional structures and reducing structural disruption caused by freezing. Further analysis of storage stability revealed that the viable count of L. acidophilus FMNS-10 exceeded 10 7 CFU/mL when stored at −20 °C for 180 days. These findings provide a comprehensive theoretical foundation for how to freeze-dry Lactobacillus acidophilus.
研究领域:
微生物学
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食品科学
✓ 已验证
产品关联
DOI
高影响因子
Nanoscale Advances
·
2024
·Vol. 6
·期 4
·1135-1144
·
IF 4.6
Xin Ke,Bingqing Xie,Jingguo Zhang,Jianwei Wang,Weiying Li,Liqing Ban,Qiang Hu,Huijun He,Limin Wang,Zhong Wang
High-purity, monodisperse, and low-oxygen submicron copper powder particles with particle sizes in the range of 100–600 nm were synthesized under alkaline conditions using ascorbic acid (C6H8O6) as a reductant and copper chloride (CuCl2·2H2O) as a copper source. The redox potential of the Cu–Cl–H2O system was obtained by calculations and plotted on pH–E diagrams, and a one-step secondary reduction process (Cu(II) → CuCl(I) → Cu2O(I) → Cu(0)) was proposed to slow down the reaction rate. The commonalities and differences in the nucleation and growth process of copper powders under methionine (Met), hexadecyl trimethyl ammonium bromide (CTAB), and sodium citrate dihydrate (SSC) as protectants and without the addition of protectants are compared, and the reaction mechanism is discussed. Among them, methionine (Met) showed excellent properties and the Cu2O(I) → Cu(0) process was further observed by in situ XRD. The synthesized copper powder particles have higher particle size controllability, dispersibility, antioxidant properties, and stability, and can be decomposed at lower temperatures (<280 °C). The resistivity can reach 21.4 μΩ cm when sintered at a temperature of 325 °C for 30 min. This green and simple synthesis process facilitates industrialization and storage, and the performance meets the requirements of electronic pastes.
研究领域:
材料科学
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化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2014
·Vol. 9
·期 6
·e100731
·
IF 3.534
Ke Xu,Ping Xu
Lactic acid is an important chemical with various industrial applications, and it can be efficiently produced by fermentation, in which Bacillus coagulans strains present excellent performance. Betaine can promote lactic acid fermentation as an effective osmoprotectant. Here, positive effect of betaine on fermentation by B. coagulans is revealed. Betaine could enhance lactic acid production by protecting l -LDH activity and cell growth from osmotic inhibition, especially under high glucose concentrations and with poor organic nitrogen nutrients. The fermentation with 0.05 g/L betaine could produce 17.9% more lactic acid compared to the fermentation without betaine. Beet molasses, which is rich in sucrose and betaine, was utilized in a co-feeding fermentation and raised the productivity by 22%. The efficient lactic acid fermentation by B. coagulans is thus developed by using betaine and beet molasses.
研究领域:
微生物学
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生物工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 7
·6696
·
IF 5.6
Yuying Hu,Xiangjian Liu,Marlene Davis Ekpo,Jiangming Chen,Xiaoxiao Chen,Wenqian Zhang,Rui Zhao,Jingxian Xie,Yongju He,Songwen Tan
The cryopreservation of red blood cells (RBCs) holds great potential for ensuring timely blood transfusions and maintaining an adequate RBC inventory. The conventional cryoprotectants (CPAs) have a lot of limitations, and there is an obvious need for novel, efficient, and biocompatible CPAs. Here, it is shown for the first time that the addition of dimethylglycine (DMG) improved the thawed RBC recovery from 11.55 ± 1.40% to 72.15 ± 1.22%. We found that DMG could reduce the mechanical damage by inhibiting ice formation and recrystallization during cryopreservation. DMG can also scavenge reactive oxygen species (ROS) and maintain endogenous antioxidant enzyme activities to decrease oxidative damage during cryopreservation. Furthermore, the properties of thawed RBCs were found to be similar to the fresh RBCs in the control. Finally, the technique for order performance by similarity to ideal solution (TOPSIS) was used to compare the performance of glycerol (Gly), hydroxyethyl starch (HES), and DMG in cryopreservation, and DMG exhibited the best efficiency. This work confirms the use of DMG as a novel CPA for cryopreservation of RBCs and may promote clinical transfusion therapy.Keywords:cryopreservation;dimethylglycine;red blood cells;cryoprotectant
研究领域:
血液学
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生物技术
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低温生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Pharmaceutics
·
2019
·Vol. 11
·期 10
·539
·
IF 4.773
You Xu,Yiyue Guo,Yuqi Yang,Yingying Meng,Xuejun Xia,Yuling Liu
Deformable nanovesicles have been extensively investigated due to their excellent ability to penetrate biological barriers. However, suffering from serious physical and chemical instabilities, the wide use of deformable nanovesicles in medical applications is still limited. Moreover, far less work has been done to pursue the lyophilization of deformable nanovesicles. Here, we aimed to obtain stable deformable nanovesicles via freeze-drying technology and to uncover the underlying protection mechanisms. Firstly, the density of nanovesicles before freeze-drying, the effect of different kinds of cryoprotectants, and the types of different reconstituted solvents after lyophilization were investigated in detail to obtain stable deformable nanovesicles based on insulin-phospholipid complex (IPC-DNVs). To further investigate the underlying protection mechanisms, we performed a variety of analyses. We found that deformable nanovesicles at a low density containing 8% lactose and trehalose in a ratio of 1:4 (8%-L-T) have a spherical shape, smooth surface morphology in the lyophilized state, a whorl-like structure, high entrapment efficiency, and deformability after reconstitution. Importantly, the integrity of IPC, as well as the secondary structure of insulin, were well protected. Accelerated stability studies demonstrated that 8%-L-T remained highly stable during storage for 6 months at 25 °C. Based on in vivo results, lyophilized IPC-DNVs retained their bioactivity and had good efficacy. Given the convenience of preparation and long term stability, the use of combined cryoprotectants in a proper ratio to protect stable nanovesicles indicates strong potential for industrial production.Keywords:deformable nanovesicles;combination;cryoprotectant;mechanism;insulin
研究领域:
药剂学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Heritage Science
·
2021
·Vol. 9
·期 1
·1-13
·
IF 2.517
Xie Zaixin,Duan Zhuoqi,Zhao Zhanqiang,Li Ruheng,Zhou Bao,Yang Dequan,Hu Yongmao
Most heritage buildings and monuments are constructed out of natural stones which suffer irrevocable degradation when undergoing wet weathering, bowing, and dissolution in outdoor conditions. Self-cleaning treatments are effective for stone protecting. Herein, nano-materials which provide enhanced protectants for Marble, Qingshi and Hedishi were prepared. Inherent microscale interstices and holes exist on polished natural stone surfaces. When treated by a commercial protectant, 101S, the surfaces were hydrophobic but not self-cleaning. Colloidal protectants were prepared by dispersion of Al 2 O 3 and SiO 2 nano-powder in 101S, respectively. Self-cleaning stone surfaces were achieved after treated by the protectants, meanwhile, the interstices and holes were reserved as much as possible. The principle of the as- prepared protectants is penetrating and crosslinking on the stone surfaces as well as the inner surfaces of the interstices and holes. The reserving of the micro interstices and holes is important since the breathability of the stones is remained. The self-cleaning surfaces showed good thermal stability below 250 °C. Meanwhile, changes of color and gloss of the treated stone surfaces are in the acceptable range.
研究领域:
材料科学
·
文物保护
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2022
·
IF 4.601
Qiong Guo,Yaqiong Qin,Lining Pan,Fuwei Xie,Shaofeng Liu,Xuehui Sun,Xiaoyu Wang,Junlan Cai,Xiaodong Zhao,Huimin Liu
A bstract Tobacco alkaloids are important precursors of carcinogenic tobacco-specific nitrosamines . Therefore, accurate quantification of tobacco alkaloids is highly important. This study investigates the compensation effects of novel analyte protectants (APs) for matrix effects (MEs) to determine seven minor tobacco alkaloids (nornicotine, myosmine , anabasine , anatabine , nicotyrine , 2,3′-bipyridine, and cotinine) in mainstream cigarette smoke with high accuracy and robustness. By comparing the heights and shapes of the chromatographic peaks before and after the addition of APs to standard solutions prepared in dichloromethane and cigarette smoke solutions, the compensation effects of 12 APs and their combinations on the MEs of seven minor tobacco alkaloids were evaluated, and the best combination of 2-pyridylethylamine (2 mg/mL)+1,2-decanediol (1 mg/mL) was identified. This AP combination could effectively improve the shapes and increase the heights (by 7–371%) of chromatographic peaks for standard solutions prepared in dichloromethane and cigarette smoke solutions. Before the addition of this AP combination, the slope ratios of the calibration curves for two types of standard solutions of the seven target chemicals were 71.4–159.8%, while they were 87.4–105.6% after the addition, indicating that this AP combination reduced the matrix difference between pure solvent and cigarette smoke solution. After adding the AP combination, the standard curves of solutions prepared in dichloromethane showed good linearity ( r 2 ≥ 0.999), the spiked recoveries were between 80.9% and 119.6%, and the inter- and intraday precisions were between 1.5–9.5% and 3.1–8.5%, respectively. Three commercial cigarette samples and one mixed standard solution were also tested under four different instrument working conditions to verify the long-term accuracy and ruggedness of the approach in routine real-world analysis of the method. The results showed that the RSD values were higher (3.5–25.4%) without the AP combination than that (<6.7%) with the AP combination. Because of its high accuracy, precision, and robustness, this method has good practical prospects.
研究领域:
化学
·
毒理学
✓ 已验证
DOI
高影响因子
Applied Sciences-Basel
·
2021
·Vol. 11
·期 24
·11815
·
IF 2.679
Meiwen Lv,Xiukang Wang,Noman Walayat,Zhongli Zhang,Muhammad Hamzah Saleem,Asad Nawaz,Rana Muhammad Aadil,Shakeel Ahmed,Mario J. Simirgiotis,José M. Lorenzo,Basit Latief Jan
This study aimed to analyze the cryoprotective effect of a ovalbumin (OVA) and β-cyclodextrin (βCD) mixture (3:1, OVA/βCD) on the structure, rheology and gelling properties of myofibrillar proteins (MPs) during 90 days of frozen storage. A mixture of OVA/βCD at different concentrations (0, 2, 4, and 6%) was added to MPs and stored at −18 °C for 90 days. The addition of OVA/βCD significantly decreased the sulfhydryl contents while it increased the surface hydrophobicity, which was closely connected with tertiary structural changes. Circular dichroism analysis showed that the addition of OVA/βCD enhanced the stability of the secondary structure by inhibiting the decline in the α-helix. Rheological properties analysis indicated that 6% OVA/βCD treatment showed better storage modulus (G’) and loss modulus (G”). In addition, treatment of OVA/βCD showed better gel forming properties than the control group (0%), helping to form a homogeneous and denser gel network. The results proved that 6% OVA/βCD could be act as a promising cryoprotectant, which can improve the structure and gel behavior ofCulter alburnusMPs during frozen storage. Moreover, OVA/βCD could be a potential alternative to conventional cryoprotectants at the industrial level to increase the economic and commercial values of seafood products.Keywords:myofibrillar proteins;ovalbumin;β-cyclodextrin;cryoprotectant;frozen storage
研究领域:
食品科学
·
蛋白质化学
✓ 已验证
产品关联
DOI
高影响因子
Foods
·
2021
·Vol. 10
·期 3
·529
·
IF 4.35
Jinsong Wang,Lanming Chen
Probiotics are considered as desirable alternatives to antibiotics because of their beneficial effects on the safety and economy of farm animals. The protectant can ensure the viability of probiotics, which is the prerequisite of the beneficial effects. The objective of this study was to evaluate the effects of a novel nano-protectant containing trehalose, skim milk powder, phytoglycogen nanoparticles, and nano-phytoglycogen Pickering emulsions on the viability ofLactobacillus caseiK17 under different conditions. The results indicated that the optimal concentration of the carbohydrate substrate was determined to be 10% skim milk powder (w/w) instead of trehalose. The combination of 10% skim milk powder (w/w), 1% phytoglycogen nanoparticles (w/w), and 10% Pickering emulsions (w/w) was selected as the optimal component of the protectant. Trilayer protectants with an optimal component had a more significant protective effect on the bacteria than that of the monolayer and bilayer protectants, or the control in feed storage, freeze-drying, and simulated gastrointestinal environment. A scanning electron microscope was used to monitor the morphological characteristics of the protectants for different layers onL. casei. In conclusion, the trilayer protectant exhibited a substantial effect onL. caseiduring storage and consumption, which could be used in the feed and functional food.Keywords:Lactobacillus casei;protectants;probiotics;phytoglycogen nanoparticle;pickering emulsions
研究领域:
微生物学
·
食品科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）