LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
·
IF 6.6
Jingwen Li,Hui Yu,Lijuan Wang,Qing Ma,Dongkun Zhao,Baocheng Xu,Xinjing Dou,Lili Liu
This study investigated the protective effects of five colored PET films against phytosterol (PS) oxidation in vegetable oil. The transparent film demonstrated the lowest inhibition rates for the formation of phytosterol oxidation products (POPs) among the five coloured films, which inhibited PS oxidation solely by reducing light intensity. In contrast, the blue film exhibited superior light barrier characteristics, effectively blocking wavelengths corresponding to ultraviolet light and chlorophyll excitation bands. This dual mechanism significantly reduced both auto-oxidation and type II photooxidation pathways of PS. Consequently, the blue film increased inhibition rates for POPs (7α-OH-PS, 7β-OH-PS, 5α,6α-epoxy-PS, 7-keto-PS, 6β-OH-PS, and 5β,6β-epoxy-PS) from 22.12 %, 13.66 %, 15.08 %, 12.16 %, 12.93 %, and 10.70 % to 76.60 %, 75.29 %, 79.40 %, 75.41 %, 77.56 %, and 75.33 %, respectively. Mechanistic analysis revealed that the blue film's enhanced protection for PS oxidation originated from its inhibition of C7 pathway-mediated POP generation. While red, yellow, and green films operated through similar protective mechanisms, their efficacy varied significantly. The overall protective performance was ranked as follows: red ≈ blue > yellow > green > transparent. These findings provide critical insights for developing optimized packaging solutions to inhibit PS oxidation in lipid-containing food products.
研究领域:
食品科学
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植物化学
✓ 已验证
产品关联
DOI
高影响因子
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
·
2025
·Vol. 39
·期 9
·e9995
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IF 1.7
Guixiang Yang,Yalan Cui,Xingxing Zong,Qian Jin,Yi Zhang,Liqin Li,Dongxin Liu,Xuejun Chen,Chen Wang,Jingchen Wei
Rationale N -[2-Hydroxy-3,5-dimethylbenzilidene]-Hup A (H14) is a derivative of huperzine A (Hup A) that demonstrates superior protective effects against soman (GD) compared to Hup A. This study aims to evaluate the protective efficacy of H14 pretreatment against GD in rats and to provide an analytical framework for the pharmacokinetic evaluation of H14 in experimental animals. Methods The study employed protective ratios (PR) as an evaluation criterion to assess the efficacy of H14 and Hup A in preventing GD in vivo. Liquid–liquid extraction techniques were utilized to extract H14 and its metabolite, Hup A, from plasma. The extracted plasma samples were then analyzed using an ultra-high-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method for the simultaneous quantification of H14 and Hup A. Results The PR values for the 6- and 12-h Hup A groups were 1.26 and 1.08, respectively. In contrast, the 6, 12, and 24-h H14 groups demonstrated PR values of 2.81, 1.98, and 1.18, respectively, indicating extended protective capabilities compared to Hup A. All validation parameters for the UHPLC–MS/MS method, including linearity, specificity, precision, accuracy, matrix effect, and stability, met the acceptance criteria established by FDA guidelines. The pharmacokinetic analysis indicates that H14, after conversion to Hup A in vivo, significantly extends the duration of Hup A concentrations in the body, leading to more effective prevention of GD poisoning compared to Hup A alone. Conclusions H14 demonstrates superior efficacy in preventing GD poisoning compared to Hup A. Furthermore, this analytical approach offers a reliable and efficient method for the pharmacokinetic evaluation of H14 in experimental animals.
研究领域:
药理学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Animal Diseases
·
2022
·Vol. 2
·期 1
·1-9
·
IF 0
Lu Ying,Liu Zehui,Li Yingxiang,Deng Zhuofan,Fang Weihuan,He Fang
Porcine circovirus type 2 (PCV2) is the main causative agent of porcine circovirus-associated diseases, and it causes substantial economic losses in the swine industry each year. It is crucial to develop an effective vaccine against the circulating strain PCV2d, which is prone to substantial degrees of mutation. In this study, a truncated form of flagellin (tFlic: 85-111 aa) was inserted into the C-terminal sequence of 2dCap, and Western blotting results showed that recombinant Cap-tFlic VLPs were successfully expressed. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) data indicated that purified recombinant Cap-tFlic fusion proteins existed in the form of polymers and that tFlic could not affect the formation and internalization of VLPs. Integrated Cap-tFlic VLPs induced the expression of antigen presentation-related factors (MHC-II and CD86) by bone marrow-derived dendritic cells (BM-DCs), and the expression of TLR5-related factors (TNF-α) was dramatically elevated. Mice intramuscularly immunized with Cap-tFlic VLPs exhibited significantly higher levels of Cap-specific antibodies and neutralizing antibodies than mice immunized with wild-type Cap VLPs. The data obtained in the current study indicate that Cap-tFlic may be a candidate for a subunit vaccine against PCV2 in the future.
研究领域:
兽医学
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免疫学
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病毒学
✓ 已验证
DOI
JOURNAL OF MICROBIOLOGY
·
2020
·Vol. 58
·期 8
·675-686
·
IF 2.845
Zou Peizhuo,Yang Qian,Wang Hailiang,Xie Guosi,Cao Zhi,Chen Xing,Gao Wen,Huang Jie
Acute hepatopancreatic necrosis disease (AHPND) is one of the most significant bacterial diseases in global shrimp culture, causing severe economic losses. In the present study, we carried out in vitro antimicrobial tests to investigate the disinfection efficacy of 14 common disinfectants toward different AHPND-causing Vibrio spp., including eight isolates of V. parahaemolyticus , four isolates of V. campbellii , and one isolate of V. owensii . Polyhexamethylene biguanidine hydrochloride (PHMB) was revealed to possess the strongest inhibitory activity. Through analyzing and evaluating the results of antimicrobial tests and acute toxicity test, we selected PHMB and hydrogen peroxide (H 2 O 2 ) for further clinical protection test. Clinical manifestations indicated that both PHMB (2 mg/L and 4 mg/L) and H 2 O 2 (12 mg/L) could effectively protect juvenile Penaeus vannamei from the infection of V. parahaemolyticus isolate Vp 362 at 10 6 CFU/ml, and the survival rate was over 80%. When the bacterial concentration was reduced to 10 5 CFU/ml, 10 4 CFU/ml, and 10 3 CFU/ml, the survival rate after treated by 1 mg/L PHMB was 64.44%, 93.33%, and 100%, respectively. According to the results, PHMB and H 2 O 2 showed a lower toxicity while a better protection activity, particularly against a lower concentration of the pathogens. Therefore, these two disinfectants are proved to be promising disinfectants that can be applied to prevent and control AHPND in shrimp culture. Moreover, the methods of this study also provided valuable information for the prevention of other important bacterial diseases and suggested a reliable means for screening potential drugs in aquaculture.
研究领域:
水产养殖学
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微生物学
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病害防控
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Cosmetic Dermatology
·
2022
·Vol. 22
·期 3
·1046-1062
·
IF 2.189
Shasha Wang,Yuxin Shi,Jiapeng Ma,Zhuofei Ye,Miaomiao Yao,Jing Shang,Jianping Liu
Dragon's Blood is a member of the Chinese medicinal herb, having anti-oxygen and anti-inflammatory activity for the photoprotective effect. However, the poor water solubility of raw Dragon's Blood powder has limited its intradermal delivery process. In this study, we evaluated nanosuspensions to enhance intradermal delivery of Dragon's Blood exerting a photoprotective effect. The prepared nanosuspension was added to a composite hydrogel patch matrix for better skin application. In the present research, we used biocompatible materials hyaluronic acid and amino acid surfactants as nanosuspension stabilizers and agar/gelatin/sodium polyacrylate as hydrogel patch matrix. The prepared Dragon's Blood nanosuspension had a particle size of 447.0 ± 48.6 nm. The micro-structures morphology and viscoelasticity characteristics by SEM and rheological testing confirmed a sufficient crosslinked hydrogel network. The skin retention amount of Dragon's Blood nanosuspension was 1.48 times of raw Dragon's Blood powder water suspension, and the skin penetration amount of Dragon's Blood nanosuspension was only about 1/3 of Dragon's Blood DMSO solution. In the UVB-irradiated HaCaT cell phototoxicity model, Dragon's Blood nanosuspension also significantly increased cell viability by about 1 time of the model group and decreased the production of reactive oxygen species about 1/2 times of model group. In vivo safety and efficiency evaluation experiment illustrated that DB-NS hydrogel patch processes have favorable safety and photoprotective effect with no skin irritancy and phototoxicity. Furthermore, DB-NS and DB-NS hydrogel patches could protect skin from UVA and UVB irritating skin reactions. Overall, our study of the combined use of biocompatible and biodegradable materials as excipients of nanosuspension and hydrogel patch could be used as an effective additive of Intradermal delivery and skin photoprotection.
研究领域:
药剂学
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纳米技术
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皮肤病学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）