INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2025
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IF 8.5
Xinyu Qian,Yuqian Guo,Yumeng Zhao,Leilei Hou,Yun Cheng,Linghua Chen,Shuo Yang,Hongjie Zhang
The swelling performance of the biological macromolecules of cellulose in aqueous N -methyl-morpholine-N-oxide (NMMO) solution is a critical factor in improving cellulose dissolution efficiency during lyocell process. We focused on the diffusion behavior of NMMO solvent during cellulose swelling and dissolution process using a novel centrifugation method, clarifying the relationship between NMMO retention value, fiber structural properties, and dissolution efficiency. An effective quantitative index for evaluating the swelling performances of cellulose is NMMO-retention, which was defined by investigating the removal of aqueous NMMO solution from swollen cellulose fibers using centrifugal method. Based on the variation of NMMO-retention and centrifugal force at 500 and 3000 g , the removal process was divided into three stages: rapid removal stage (0–500 g), slow removal stage (500–3000 g), and residual removal stage (3000–5000 g). In addition, the relationship between fiber structural properties (fiber length, width, fine content, and specific surface area) and swelling-dissolution performance was established. The results indicate that the NMMO-retention of cellulose is a comprehensive reflection of the fiber structural characteristics, and it shows an obvious negative correlation with the dissolution time of cellulose. The cellulose that shows the highest NMMO-retention (729.5 %) after swelling has the shortest dissolution time (20 min) during subsequent dissolution.
研究领域:
材料科学
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生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2021
·
IF 8.071
Shuai Guo,Chaoxian Wei,Yaxian Zhu,Yong Zhang
Plant leaf cuticles play a critical role in the accumulation and transport of atmospheric polycyclic aromatic hydrocarbons (PAHs). The relationship between the distribution and retained amount of PAHs on the leaf cuticles and the leaves micro-zone structures is still unclear. In this study, a confocal microscopic fluorescence spectral analysis (CMFSA) system with a spatial resolution of 200 nm was established as a direct and noninvasive means to determine the microscopic distribution and quantify the retained amount of benzo[a]pyrene (B[a]P) at Aegiceras corniculatum ( Ac ), Kandelia obovata ( Ko ) and Avicennia marina ( Am ) leaf cuticle micro-zones (0.096 mm 2 ). The linear ranges for the established method were 10–1900 ng spot −1 for Ac , 15–1700 ng spot −1 for Ko and 30–1800 ng spot −1 for Am , and the detection limits were 0.06 ng spot −1 for Ac , 0.06 ng spot −1 for Ko and 0.07 ng spot −1 for Am . Notably, B[a]P formed clusters and unevenly distributed at the leaf cuticles. On the adaxial cuticles, B[a]P was mainly accumulated unevenly along the epidermis cell wall, and it was also distinctively distributed in the secretory cells around salt glands for Ac and Am . On the abaxial leaf cuticles, B[a]P was concentrated in the salt glands and stomata apart from being unevenly distributed in the epidermis cell wall. Moreover, the amount of B[a]P retained presented a negative correlation with the polarity of leaf cuticles, which resulted in the amount of B[a]P retained on the adaxial leaf cuticles being significantly higher than that on abaxial leaf cuticles. Our results provide a potential in situ method for investigating the distribution and retained amount of PAHs at plant leaf surface micro-zones, which would contribute to further studying and understanding the mechanism of migration and transformation of PAHs by plant leaves from a microscopic perspective.
研究领域:
植物学
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环境科学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Animal Nutrition
·
2024
·
IF 6.1
Jinming Hu,Shuo Zhang,Mengmeng Li,Guangyong Zhao
This study investigated the effects of β-alanine (β-Ala) on ruminal fermentation, nutrient digestibility, nitrogen (N) metabolism, plasma biochemical parameters, and rumen bacterial communities in beef steers. Six steers with initial liveweight of 252.8 ± 5.2 kg and 3 treatments of supplementing with 0, 30, or 60 g β-Ala per day to the basal diet were allocated in a replicated 3 × 3 Latin square design. Each experimental period was 20 d, of which the first 15 d were for adaptation and the subsequent 5 d were for sampling. The results showed that β-Ala linearly increased the ruminal concentration of microbial crude protein (MCP) ( P = 0.002), but it did not affect the ruminal concentrations of ammonia N and total volatile fatty acids ( P > 0.10). β-Ala also linearly increased the dry matter (DM) ( P = 0.009), organic matter (OM) ( P = 0.017) and crude protein (CP) ( P = 0.043) digestibility, tended to decrease the acid detergent fiber digestibility ( P = 0.077), but it did not affect the neutral detergent fiber digestibility ( P = 0.641). β-Ala quadratically increased the relative abundance of ruminal Bacteroidota ( P = 0.021) at the phylum level, and increased Prevotella ( P = 0.028), and Prevotellaceae_UCG-003 ( P = 0.014) and decreased the relative abundance of NK4A214_group ( P = 0.009) at the genus level. Feeding steers with β-Ala linearly increased the urinary N excretion ( P = 0.006) and urea excretion ( P = 0.002) and the N retention ( P = 0.004), but it did not affect the N utilization efficiency ( P = 0.120). β-Ala quadratically increased the plasma concentrations of albumin ( P = 0.048) and linearly increased the plasma concentration of insulin-like growth factor-1 ( P < 0.001) and the total antioxidant capacity ( P = 0.011). In summary, dietary supplementation with β-Ala improved the rumen MCP supply and increased the digestibility of DM, OM, CP and the N retention. Further research is necessary to verify the ruminal degradability of β-Ala and to investigate the mechanism of the impact of absorbed β-Ala on the anti-oxidative ability of the steers.
研究领域:
动物营养学
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微生物学
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反刍动物生理学
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DOI
PubMed
高影响因子
Journal of Cosmetic Dermatology
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2022
·Vol. 22
·期 3
·1046-1062
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IF 2.189
Shasha Wang,Yuxin Shi,Jiapeng Ma,Zhuofei Ye,Miaomiao Yao,Jing Shang,Jianping Liu
Dragon's Blood is a member of the Chinese medicinal herb, having anti-oxygen and anti-inflammatory activity for the photoprotective effect. However, the poor water solubility of raw Dragon's Blood powder has limited its intradermal delivery process. In this study, we evaluated nanosuspensions to enhance intradermal delivery of Dragon's Blood exerting a photoprotective effect. The prepared nanosuspension was added to a composite hydrogel patch matrix for better skin application. In the present research, we used biocompatible materials hyaluronic acid and amino acid surfactants as nanosuspension stabilizers and agar/gelatin/sodium polyacrylate as hydrogel patch matrix. The prepared Dragon's Blood nanosuspension had a particle size of 447.0 ± 48.6 nm. The micro-structures morphology and viscoelasticity characteristics by SEM and rheological testing confirmed a sufficient crosslinked hydrogel network. The skin retention amount of Dragon's Blood nanosuspension was 1.48 times of raw Dragon's Blood powder water suspension, and the skin penetration amount of Dragon's Blood nanosuspension was only about 1/3 of Dragon's Blood DMSO solution. In the UVB-irradiated HaCaT cell phototoxicity model, Dragon's Blood nanosuspension also significantly increased cell viability by about 1 time of the model group and decreased the production of reactive oxygen species about 1/2 times of model group. In vivo safety and efficiency evaluation experiment illustrated that DB-NS hydrogel patch processes have favorable safety and photoprotective effect with no skin irritancy and phototoxicity. Furthermore, DB-NS and DB-NS hydrogel patches could protect skin from UVA and UVB irritating skin reactions. Overall, our study of the combined use of biocompatible and biodegradable materials as excipients of nanosuspension and hydrogel patch could be used as an effective additive of Intradermal delivery and skin photoprotection.
研究领域:
药剂学
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纳米技术
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皮肤病学
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药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）