MICROCHEMICAL JOURNAL
·
2023
·
IF 4.8
Heng Zhang,Keqin Deng,Xinmei Qian,Chunxiang Li,Jinglun Wang,Qingfeng Yi,Haowen Huang,Hu Zhou
Hexavalent chromium (Cr(VI)) is highly poisonous and can accumulate in the environment. In this work, we developed a photoelectrochemical (PEC) sensor to detect Cr(VI) content. Firstly, a bean-like AgBr 0.8 I 0.2 -Ag-CNTs composite was synthesized through light reduction and hydrothermal method. The physicochemical characterization was investigated and the results revealed that AgBr 0.8 I 0.2 -Ag-CNTs possessed a wide visible light absorption range and excellent photoelectrochemical activity. Subsequently, a “signal-off” PEC sensor based on AgBr 0.8 I 0.2 -Ag-CNTs as cathodic sensing platform and K 3 [Fe(CN) 6 ] as photoelectron acceptor was proposed. After photoelectrochemical reduction of Cr(VI) ions, Cr(VI) was reduced to cover AgBr 0.8 I 0.2 -Ag-CNTs in the form of Cr(OH) 3 , which severely blocked the photoelectron transfer from AgBr 0.8 I 0.2 -Ag-CNTs to K 3 [Fe(CN) 6 ] and resulted in a falling photocurrent. Compared with previous PEC sensors based on the direct reduction of Cr(VI) to Cr 3+ , the proposed sensor showed a wider linear range of 0.1 nM ∼ 5.0 µM and a lower limit of detection (LOD) of 50 pM.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2024
·Vol. 16
·期 9
·1330-1340
·
IF 2.7
Cuizhong Zhang,Qiang Wang,Chuanze Zhong,Ye Yang,Xuexue Liang,Peican Chen,Liya Zhou
Designing a simple and sensitive photoelectrochemical (PEC) sensor is crucial to addressing the limitations of routine analytical methods. The sensitivity of the PEC sensor, however, relies on the photoelectric material used. In this manuscript, composites of MoS2/rGO (MG) with a large area and layered structure are prepared by simple steps. This material exhibits sensitivity to visible light and demonstrates outstanding photoelectric conversion performance. The constructed PEC aptasensor using this material to detect aflatoxin B1 (AFB1) shows significantly higher sensitivity and stability compared to similar sensors. This may be attributed to the presence of surface defects in MoS2, which provide more active sites for photocatalysis. Additionally, graphene oxide (GO) is reduced to rGO by thiourea and forms a heterojunction with MoS2, enhancing charge carrier separation and interfacial electron transfer. Our research has revealed that the photocurrent intensity of the aptamer electrode decreases with an increase in AFB1 concentration, resulting in a “signal-off” PEC aptasensor. The detection limit of this aptasensor is 2.18 pg mL−1, with a linear range of 0.001 to 100 ng mL−1. This result will also provide a reference for the study of other mycotoxins in food.
研究领域:
食品科学
·
生物传感
·
光电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Yihan Wang,Jinfeng Quan,Jialei Zhang,Kejing Huang,Xuemei Wang,Hui Jiang
The fragments of molecular motors can be transported and operated autonomously along a track. Most molecular walker systems are assembled from DNA building modules, exhibiting great potential applications in biocomputing, drug transport, sensing, etc. Hereby, we report a DNA walker driven by endonuclease Nt.BbvC I that can autonomously induce “signal off” sensing platform for highly sensitive detection of tumor cells. DNA density and trajectory conformation can affect DNA walker properties. Molybdenum selenide@gold nanoparticles (MoSe 2 @AuNPs) are modified on the electrode surface as a scaffold to accommodate more hairpin probe (H1) and provide a good interface for DNA walking. Magnetic beads are used to enrich target tumor cells, release single-stranded DNA (S1) and trigger DNA walker-based amplification strategy. Upon addition of tumor cells, more DNA-methylene blue (DNA-MB) is cracked from the electrode surface. Benefiting from efficient electrode modification, magnetic enrichment, DNA walker and enzyme-assisted amplification, an ultra-high sensitivity electrochemical cell analysis is realized, with a detection limit of 2 cells mL −1 . More importantly, this DNA walker strategy is available for tumor cell analysis in clinical samples, suggesting the potential diagnostic applications.
研究领域:
生物传感
·
分子生物学
·
医学诊断
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2015
·
IF 4.097
Yanli Zhang,Shixiu Xiao,Haizhen Li,Hongjun Liu,Pengfei Pang,Hongbin Wang,Zhan Wu,Wenrong Yang
A novel strategy for selective and sensitive electrochemical lead ion (Pb 2+ ) biosensor was developed based on the single-stranded DNAzyme catalytic beacon. A DNAzyme that requires Pb 2+ for activation was selected and labeled with redox-active ferrocene (Fc) for signal transducer. The Fc-labeled single-stranded DNAzyme (Fc-ssDNAzyme) was self-assembled through S Au bonding on a gold electrode surface. In the presence of Pb 2+ , the ssDNAzyme was activated and catalyzed the hydrolytic cleavage of the substrate strand, resulting in the removal of the substrate strand along with the Fc from the Au electrode surface. The dissociation of Fc caused a decrease of electrochemical signal (“signal-off”). Under the optimal conditions, the electrochemical signal of Fc decreased directly with the increasing Pb 2+ concentration, exhibiting a linear response in the range of 0.5 nM to 5 μM with a detection limit of 0.25 nM. This strategy is simple, sensitive and selective with the minimal reagents and working steps, thereby holds great potential for Pb 2+ detection in real environmental sample analysis.
研究领域:
分析化学
·
环境科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Zenghui Liu,Jinyan Xue,Luyao Chen,Lele Ma,Huaixia Yang,Yaping Zhang,Mingsan Miao
A new, simple and low-cost electrochemical analysis approach employing click polymerization as the signal amplification strategy was developed for the highly sensitive assay of aflatoxin B 1 (AFB 1 ). First, the aptamer served as the capture probe for AFB 1 self-assembled on the surface of the Au electrode via Au-S bonding. After complementary DNA (c-DNA) modified with azide groups was hybridized with the aptamer, a massive click polymer was introduced as a signal tag via click chemistry. Finally, the captured AFB 1 competed with the c-DNA to bind the aptamer and the c-DNA was shed from the electrode surface and the current intensity was reduced. The electrochemical aptamer sensor is easy to operate, selective and sensitive, and also exhibits excellent reliability in detecting AFB 1 in actual samples. A rapid, simple, efficient and sensitive platform for AFB 1 analysis is proposed in this work, and the strategy can be further extended to trace analysis of other analytes, safety detection, environmental monitoring and disease diagnosis after replacing the corresponding capture probes, with promising applications in the fields of food hygiene and safety.
研究领域:
分析化学
·
生物化学
·
食品安全
·
环境监测
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Sihua Chen,Aimin Li,Lizhi Zhang,Jingming Gong
Driven by the urgent demand for the determination of low level perfluorooctanoic acid (PFOA) present in environment, a novel electrochemiluminescence (ECL) sensor has been first developed for the detection of PFOA using the molecularly imprinted polypyrrole modified two-dimensional ultrathin g-C 3 N 4 (utg-C 3 N 4 ) nanosheets as a cathodic ECL emitter with S 2 O 8 2− as coreactant. The prepared molecularly imprinted polymer (MIP) functionalized utg-C 3 N 4 nanosheets (MIP@utg-C 3 N 4 ) exhibit a stable and significantly amplified ECL signal. It is found that the targets of PFOA could be efficiently oxidized by the electro-generated strong oxidants of SO 4 − (from the reduction of coreactant S 2 O 8 2− ), thus leading to a low yield of the excited utg-C 3 N 4 (g-C 3 N 4 *) and finally a decrease in ECL signal. Based on this, a highly sensitive and selective MIP@utg-C 3 N 4 -based signal-off ECL sensor is developed for sensing PFOA. Such a newly designed ECL sensor exhibits highly linear over the PFOA concentration in two ranges, from 0.02 to 40.0 ng mL −1 and 50.0–400.0 ng mL −1 . The detection limit (S/N = 3) is estimated to be 0.01 ng mL −1 (i.e. 0.01 ppb), comparable to the results obtained by using well-established liquid chromatography-tandem mass spectrometry (LC-MS/MS). Toward practical applications, this low-cost and sensitive assay was successfully applied to measure PFOA in real water samples, showing fine applicability for the detection of PFOA in real samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Huimei Da,Hongyan Liu,Yingning Zheng,Ruo Yuan,Yaqin Chai
We developed a novel “signal-off” photoelectrochemical (PEC) aptasensor based on an aptamer bridged DNA network structure for the sensitive detection of vascular endothelial growth factor (VEGF 165 ), using g-C 3 N 4 as photoactive material. The DNA network provides an excellent platform for the immobilization of methylene blue (MB), which can facilitate the electron transport through the DNA helix structure and suppress the recombination of electron-hole pairs generated by g-C 3 N 4 . In the presence of the target VEGF 165 , the DNA network can be destroyed adequately by the recognition between VEGF 165 and the aptamer, resulting in the release of MB. Therefore, the originally enhanced electron transfer process could be inhibited, leading to a remarkable decrease of the photocurrent . A good linear relationship between the PEC signal and the logarithm of VEGF 165 concentration over the range of 100 fM to 10 nM with a detection limit of 30 fM can be obtained. Our concept can be easily extended to develop aptasensors for the sensitive detection of different targets by triggering the release of the payloads from their corresponding aptamer bridged DNA networks.
研究领域:
生物化学
·
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Dong Liang,Junjun Luo,Yaliang Huang,Xiang Liang,Zaichun Zhou,Jianxiu Wang,Xiaoqing Qiu
In this study, we report the development of indirect Z-scheme heterojunction to construct a cathodic photoelectrochemical (PEC) sensor for highly-selective detection of endogenous H 2 S using AgCl-Ag core–shell nanostructures coupled with porous carbon nitride (PCN) as cathode materials. As an electron mediator, Ag on the Z-scheme heterojunction transformed to Ag 2 S in the presence of H 2 S, resulting in the destruction of original charge-carrier migration pathway. Meanwhile, photo-induced charges on the then formed AgCl-Ag 2 S/PCN cannot be as effectively separated as they are on AgCl-Ag/PCN due to the straddling energy band alignments. A decrease in cathodic signal may be expected. Consequently, the H 2 S concentration could be quantitated by such corresponding decrease, which thus underlines a novel signal-off cathodic PEC bioanalysis strategy. More importantly, the ‘Z-scheme’ PEC sensor based on AgCl-Ag/PCN exhibits analytical performance with high sensitivity, showing its potential to be applied to the areas of biomedical and health care etc.
研究领域:
分析化学
·
环境科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2015
·
IF 5.636
Kai Yan,Yong Liu,Yaohua Yang,Jingdong Zhang
A novel cathodic “signal-off” strategy was proposed for photoelectrochemical (PEC) aptasensing of oxytetracycline (OTC). The PEC sensor was constructed by employing a p-type semiconductor BiOI doped with graphene (G) as photoactive species and OTC-binding aptamer as a recognition element. The morphological structure and crystalline phases of obtained BiOI-G nanocomposites were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD). The UV–visible absorption spectroscopic analysis indicated that doping of BiOI with graphene improved the absorption of materials in the visible light region. Moreover, graphene could facilitate the electron transfer of BiOI modified electrode. As a result, the cathodic photocurrent response of BiOI under visible light irradiation was significantly promoted when a suitable amount of graphene was doped. When amine-functionalized OTC-binding aptamer was immobilized on the BiOI-G modified electrode, a cathodic PEC aptasensor was fabricated, which exhibited a declined photocurrent response to OTC. Under the optimized conditions, the photocurrent response of aptamer/BiOI-G/FTO was linearly proportional to the concentration of OTC ranging from 4.0 to 150 nM, with a detection limit (3S/N) of 0.9 nM. This novel PEC sensing strategy demonstrated an ultrasensitive method for OTC detection with high selectivity and good stability.
研究领域:
分析化学
·
纳米技术
·
环境科学
·
生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2018
·
IF 5.667
Xiao-Lei Zhang,Xue Li,Xiao-Tong Li,Ye Gao,Fang Feng,Gong-Jun Yang
The Au nanoclusters@graphene quantum dots nanocomposite as an electrochemiluminescence (ECL) luminophore was synthesized through amide coupling reaction between Au nanoclusters (Au NCs) and graphene quantum dots (GQDs). The ECL intensity of the as-prepared Au NCs@GQDs was enhanced for 2.6-fold compared with that of Au NCs or 40-fold compared with that of GQDs, which was due to the ECL resonance energy transfer between GQDs as a donor and Au NCs as an acceptor, and excellent electrical conductivity of GQDs. In addition, the synergistic effect likewise improved stability of the ECL signal. A type of “signal-off” solid-state ECL sensor for detection of pentoxifylline was fabricated based on the selective quenching effect of pentoxifylline on the ECL signal of Au NCs@GQDs in alkaline medium. The sensor showed excellent sensitivity and wide linearity for pentoxifylline determination in the range from 7.0 × 10 −7 to 1.2 × 10 -4 mol/L with the detection limit of 9.0 × 10 -8 mol/L (S/N = 3). The proposed method was successfully applied for pentoxifylline detection in pharmaceutical tablet with satisfactory results.
研究领域:
分析化学
·
纳米技术
·
药物分析
✓ 已验证
产品关联
DOI
高影响因子
FOOD CONTROL
·
2019
·
IF 4.248
Yu Hou,Gaozhe Cai,Lingyan Zheng,Jianhan Lin
The key to prevent and control the spread of foodborne diseases is rapid screening and early warning of pathogenic bacteria in foods . In this study, a microfluidic biosensor using magnetic nanoparticles (MNPs) and catalases was developed for rapid and sensitive detection of Salmonella typhimurium. First, the MNPs modified with anti- Salmonella monoclonal antibodies were used to separate and enrich target bacteria from sample background to form magnetic bacteria, which were reacted with polystyrene microspheres (PSs) modified with anti- Salmonella polyclonal antibodies and catalases to form enzymatic bacteria. Then, the enzymatic bacteria were injected into the capillary in microfluidic chip and captured by high gradient magnetic field. After washing to remove unbound PSs, hydrogen peroxide was finally injected and catalyzed by the catalases on enzymatic bacteria to produce oxygen gap in the capillary, leading to electrical signal-off. The change of electrical voltage was found to have a good linear relationship with the concentration of target bacteria from 3.7 × 10 1 to 3.7 × 10 6 CFU/mL. The proposed biosensor was able to detect Salmonella as low as 33 CFU/mL within 2 h, and could be further combined with microfluidics to develop a lab-on-a-chip system for in-field detection of foodborne bacteria to ensure food safety.
研究领域:
生物传感器
·
微流控技术
·
食品安全
·
病原体检测
·
磁分离
·
酶催化
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2019
·
IF 5.256
Mengsi Li,Caixia Wang,Liming Chen,Defang Liu
Electrochemiluminescence resonance energy transfer (ECL-RET) between quantum dots (QDs) was firstly proposed. In this work, boron nitride quantum dots (BNQDs) as the donor and nitrogen doped graphene quantum dots (NGQDs) as the acceptor were confirmed by the absorption spectrum , the emission spectrum and fluorescence spectrum . Based on the reaction between FA and the SO 4 •− in the ECL system of NGQDs/BNQDs/K 2 S 2 O 8 , the ECL sensing platform for FA was successfully constructed. Surprisingly, a stable and strong ECL signal was obtained based on the RET, which was used for signal-off detection of FA in the presence of coreactant K 2 S 2 O 8 . Notably, about 10-fold enhancement was observed compared with the absence of BNQDs. The proposed sensor showed wide linear ranges of 1.0 × 10 −11 M to 1.0 × 10 −4 M and a low detection limit of 5.13 × 10 −12 M. Simultaneously, the sensor was successfully applied to detection of FA in human serum samples with excellent recoveries. Therefore, the NGQDs/BNQDs system provided a new perspective for development of novel ECL-RET sensors.
研究领域:
分析化学
·
材料科学
·
生物化学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）