Materials Today Chemistry
·
2025
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IF 6.7
Xiaoyu Tian,Jinwei Bao,Lei Song,Bo Zhang,Junsheng Yang,Liangbo Sun,Houchang Pei,Qiang Cao,Qianqian Lin
Noble-metal-free surface-enhanced Raman spectroscopy (SERS) substrates have the advantages, such as abundant resources, uniformity, good biocompatibility, and chemical stability, and they have sparked widespread attention in food safety assessment. However, the lack of controllable synthesis and fabrication method limits their performance in practical applications, especially in the production of high SERS activity substrates. In this study, we introduce a femtosecond laser direct writing technique to precisely manipulate and modify microstructures, successfully fabricating micro-rings of different sizes. Benefiting from the localized field enhancement effect of the optical microcavity, the Sb 2 S 3 micro ring exhibits significantly enhanced Raman signals compared to the unmodified Sb 2 S 3 thin film and the degree of signal enhancement is closely related to the size of the micro rings. Notably, the in-situ formed Sb nanoparticles during the micro ring fabrication process generate pronounced plasmonic effects. These plasmonic hot spots synergistically couple with the localized field enhancement of the optical microcavity, enabling secondary amplification of Raman signals. Furthermore, we used rhodamine 6G (R6G) and basic orange II as probe molecules, and the results indicate that the proposed Sb 2 S 3 micro-ring substrate exhibits excellent SERS performance. This study demonstrates the potential of using advanced laser technology to manufacture highly active noble-metal-free SERS substrates and highlights the importance of microstructure design in enhancing Raman signals, providing new insights for future applications in food safety detection and other fields.
研究领域:
光谱分析
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材料科学
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食品安全检测
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2024
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IF 4.1
Qingshuang Wei,Xinrong Zong,Yitao Lv,Chaoqi Wang,Jiacheng Wang,Min Zhang
Electro-activation has been an important surface modification strategy to enhance the signal of analytes on glassy carbon electrodes (GCE). However, its unique effects on certain analytes remain to be further explored. Hence, due to the electro-active hydroxyl groups (–OH), phenolic compounds were selected as the analyte to reveal it to some extent. Except for the improved electrocatalytic activity of electro-activated GCE (e-GCE) which was attributable to the improved charge transfer ability and optimized surface properties ( eg. surface area, surface roughness, and active sites), the oxygen-containing functional groups on the e-GCE surface were also identified as being of significant importance for enhanced response of phenolic compounds. After electro-activation, the newly generated C O constituted 12.81 % of the C1s spectra and 51.75 % of the O1s spectra, effectively facilitating the formation of donor–acceptor complexes between the analytes and the electrodes, thereby enhancing the adsorption of phenolic compounds. Furthermore, it was found that the structure of phenolic compounds had a remarkable impact on the response signal. The increase in the number of –OH groups involved in the electron transfer process, the increase in the number of aromatic rings, and the enhancement of the electronegativity of substituents all contributed positively to the response signal of phenolic compounds. At last, based on the great adsorption of catechol, a sensor for catechol with a sensitivity 12-fold higher than prior has been established. These results provide insights for the subsequent fabrication of green sensors for more electro-active molecules.
研究领域:
分析化学
·
电化学
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DOI
高影响因子
Biosensors-Basel
·
2020
·Vol. 10
·期 12
·211
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IF 3.24
Min-Xin Mao,Rong Zheng,Chi-Fang Peng,Xin-Lin Wei
In this work, a paper device consisted of a patterned paper chip, wicking pads, and a base was fabricated. On the paper chip, DNA–gold nanoparticles (DNA–AuNPs) were deposited and Hg2+ions could be adsorbed by the DNA–AuNPs. The formed DNA–AuNP/Hg2+nanozyme could catalyze the tetramethylbenzidine (TMB)–H2O2chromogenic reaction. Due to the wicking pads, a larger volume of Hg2+sample could be applied to the paper device for Hg2+detection and therefore the color response could be enhanced. The paper device achieved a cut-off value of 50 nM by the naked eye for Hg2+under optimized conditions. Moreover, quantitative measurements could be implemented by using a desktop scanner and extracting grayscale values. A linear range of 50–2000 nM Hg2+was obtained with a detection limit of 10 nM. In addition, the paper device could be applied in the detection of environmental water samples with high recoveries ranging from 85.7% to 105.6%. The paper-device-based colorimetric detection was low-cost, simple, and demonstrated high potential in real-sample applications.Keywords:paper device;signal enhancement;mercury ion;colorimetric detection
研究领域:
分析化学
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环境科学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2023
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IF 5
Ying Cheng,Chen Chen,Fang Wang,Zilin Chen
Ten-eleven translocation 1 (TET1) is a member of the TET enzyme family of dioxygenases, which plays an important role in active DNA demethylation. Therefore, the sensitive TET1 detection could help us better understand DNA methylation-demethylation in epigenetics. Here we report a detection method that consists of electrode fabrication, TET1 modification, DNA digestion, signal-on oxidoreduction, and current peak monitoring. An exquisitely designed 5′end-G-rich oligodeoxynucleotide was synthesized bearing a methylated cytosine (5-mC), which formed into hairpin dsDNA with the MspI recognition sequence (C m C GG/GGCC). Then hairpin dsDNA was fabricated onto gold nanoparticles modified glassy carbon electrode (DNA/AuNPs/GCE) via Au-S bond. The combination uses of restriction enzyme MspI and hemin converted fabricated-dsDNA into peroxidase-mimicking DNAzyme, thereby promoting the reduction of H 2 O 2 with a current peak. However, the current peak was extremely decreased once TET1 and T4 β-GT were used in advance. We confirmed a delicately linear relationship matching between the current difference and TET1 activity from 0.7 to 10.5 ng μL −1 with a detection limit of 0.027 ng μL −1 , which outcompeted the former methods at least one order of magnitudes. The TET1 activity evaluation in the existence of Bobcat339 was also tested as the proof of concept of inhibitors screening. Our strategy provides a novel, label-free, and sensitive electrochemical approach that enables us to complete both TET1 activity evaluation and potential TET1 inhibitors screening.
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
PubMed
高影响因子
ACS Sensors
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2020
·
IF 7.333
Xi Chen,Meng Wang,Gang Zhao
Monitoring of the hemostasis status is essential for therapeutic anticoagulants, undergoing surgery, cardiovascular diseases, etc. Although the clinical values of conventional blood coagulation tests have been well demonstrated, these devices have limitations such as large and expensive equipment, excessive sample volumes, long turnaround times, and difficulty in miniaturization for point-of-care use. Here, we present a novel strategy to evaluate blood hemostasis using the single-port Love-mode surface acoustic wave (SLSAW) sensor. The SLSAW sensor was designed as a plug-and-play-type unit for disposable use and operated under the harmonic resonant mode to produce frequency response to the blood coagulation cascade. Compared with a quartz crystal microbalance, Lamb wave, and film bulk acoustic resonator, the frequency shift of SLSAW was significantly increased, ranging from approximately 8960 to 10 368 kHz, which indicated enhancement of the signal-to-noise ratio. To demonstrate the feasibility of the SLSAW, studies were carried out to examine the effects of temperature and clotting reagents on coagulation times and kinetics. Activated partial thromboplastin times of plasma were validated by comparing with SYSMEX CA-7000 with the correlation (R2) as 0.996. In terms of coagulation kinetics, reaction time, clot formation time, maximum frequency shift, and clot formation rate of whole blood correlated well with corresponding parameters of the standard thromboelastography (TEG) analyzer (R2 = 0.9942, 0.9868, 0.9712, and 0.9939, respectively). The SLSAW sensor, with the advantages of low cost, small size, little sample consumption (1 μL), disposable use, and simple operation, is a promising tool for point-of-care diagnosis of hemostasis.
研究领域:
医学工程
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生物医学
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DOI
高影响因子
MOLECULES
·
2024
·Vol. 29
·期 15
·3699
·
IF 4.2
Shefeng Li,Qi Zheng,Xiaodan Liu,Peng Liu,Long Yu
Studying efficient and accurate soil heavy-metal detection technology is of great significance to establishing a modern system for monitoring soil pollution, early warning and risk assessment, which contributes to the continuous improvement of soil quality and the assurance of food safety. Laser-induced breakdown spectroscopy (LIBS) is considered to be an emerging and effective tool for heavy-metal detection, compared with traditional detection technologies. Limited by the soil matrix effect, the LIBS signal of target elements for soil heavy-metal detection is prone to interference, thereby compromising the accuracy of quantitative detection. Thus, a series of signal-enhancement methods are investigated. This study aims to explore the effect of conductive materials of NaCl and graphite on the quantitative detection of lead (Pb) in soil using LIBS, seeking to find a reliable signal-enhancement method of LIBS for the determination of soil heavy-metal elements. The impact of the addition amount of NaCl and graphite on spectral intensity and parameters, including the signal-to-background ratio (SBR), signal-to-noise ratio (SNR), and relative standard deviation (RSD), were investigated, and the mechanism of signal enhancement by NaCl and graphite based on the analysis of the three-dimensional profile data of ablation craters and plasma parameters (plasmatemperature and electron density) were explored. Univariate and multivariate quantitative analysis models including partial least-squares regression (PLSR), least-squares support vector machine (LS-SVM), and extreme learning machine (ELM) were developed for the quantitative detection of Pb in soil with the optimal amount of NaCl and graphite, and the performance of the models was further compared. The PLSR model with the optimal amount of graphite obtained the best prediction performance, with an Rp that reached 0.994. In addition, among the three spectral lines of Pb, the univariate model of Pb I 405.78 nm showed the best prediction performance, with an Rp of 0.984 and the lowest LOD of 26.142 mg/kg. The overall results indicated that the LIBS signal-enhancement method based on conductive materials combined with appropriate chemometric methods could be a potential tool for the accurate quantitative detection of Pb in soil and could provide a reference for environmental monitoring.
研究领域:
环境科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2017
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IF 5.401
Yan Wang,Panpan Wang,Ying Wu,Junwei Di
A cathodic “signal-on” method was developed for the photoelectrochemical detection of Hg 2+ ions. Here, ZnS quantum dots (QDs) capped with mercaptoacetic acid was assembled on the surface of an indium tin oxide (ITO) electrode through electrostatic interaction between poly dimethyl diallyl ammonium chloride (PDDA) and the ZnS QDs. HgS was formed on the ZnS surface by ion-exchange in the presence of Hg 2+ ions in solution. The cathodic photocurrent increased under visible light irradiation with a light-emitting diode lamp. This novel strategy exhibits high sensitivity and good selectivity owing to the low background signal and the benefits of ion-exchange technology. The cathodic photocurrent increases linearly with Hg 2+ concentration in the range 1 × 10 −11 –1 × 10 −6 M, providing a detection limit of 2 pM (S/N = 3). This sensor provides a promising new strategy for the detection of trace heavy metal ions.
研究领域:
分析化学
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环境科学
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产品关联
DOI
高影响因子
OPTICAL MATERIALS
·
2022
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IF 3.754
Xiaoxu Zhang,Chengxu Song,Shilun Li,Liang Chen,Jing Xu,Wei Liu,Shangshen Feng
The effective regulation of the absorption properties of Au nanosphere arrays, as well as effective enhancement of its SERS performance, are of great significance for its applications in the field of optical sensing. In this paper, the polyol synthesis method was used to fabricate monolayer and bilayer periodic Au nanosphere arrays, onto which monolayer graphene was coated, and the optical properties of the obtained composite structures were investigated. The results showed that the coverage of graphene enhances the absorption peak intensities of the monolayer and bilayer Au nanoarrays by up to 32.92%, and the resonance peaks were red-shifted by up to 17 nm. The Raman signals of graphene-covered monolayer and bilayer Au nanosphere composite arrays are 180 and 280 times stronger than that of pristine graphene, due to the combination of chemical enhancement and electromagnetic enhancement. And the detection sensitivity of R6G molecules by graphene-bilayer Au nanosphere composite arrays is 10 −6 M. In addition, the staggered stacking of bilayer Au nanosphere arrays can provide more plasmonic hotspots, resulting in higher Raman intensity of the substrate. These results will pave the way for the fabrication of highly efficient SERS substrates by providing various promising candidates.
研究领域:
材料科学
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光学传感
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纳米技术
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产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2014
·
IF 4.517
Xiao Li,Jie Tan,Jiekai Yu,Jiandong Feng,Aiwu Pan,Shu Zheng,Jianmin Wu
Small peptides in serum are potential biomarkers for the diagnosis of cancer and other diseases. The identification of peptide biomarkers in human plasma/serum has become an area of high interest in medical research. However, the direct analysis of peptides in serum samples using mass spectrometry is challenging due to the low concentration of peptides and the high abundance of high-molecular-weight proteins in serum, the latter of which causes severe signal suppression. Herein, we reported that porous semiconductor-noble metal hybrid nanostructures can both eliminate the interference from large proteins in serum samples and significantly enhance the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) yields of peptides captured on the nanostructure. Serum peptide fingerprints with high fidelity can be acquired rapidly, and successful discrimination of colorectal cancer patients based on peptide fingerprints is demonstrated.
研究领域:
医学
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分析化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
SENSORS
·
2024
·Vol. 24
·期 24
·7878
·
IF 3.4
Xiaoyu Su,Junbiao Chen,Shanshan Wu,Yong Qiu,Yuxiang Pan
As a crucial biomarker for the early warning and prognosis of liver cancer diseases, elevated levels of alpha-fetoprotein (AFP) are associated with hepatocellular carcinoma and germ cell tumors. Herein, we present a novel signal-on electrochemical aptamer sensor, utilizing AuNPs–MXene composite materials, for sensitive AFP quantitation. The AuNPs–MXene composite was synthesized through a simple one-step method and modified on portable microelectrodes. As signal molecules, AFP aptamers were conjugated with methylene blue (MB) and immobilized on the electrode surface. When interacting with AFP, conformational changes in the aptamer–target complex caused MB to approach the electrode, and the electrochemical signal was enhanced through signal-on mechanisms. The developed sensor demonstrated high sensitivity and selectivity for AFP, with a log-linear relationship defined as 1–300 ng/mL, and the LOD was 0.05 ng/mL (S/N = 3). The method was applied to laboratorial and real clinical samples and presented satisfactory selectivity, reproducibility, and long-term stability. The proposed high-performance sensor highlights the potential of electrochemical aptamer sensors in improving the warning capabilities in disease management.
研究领域:
生物医学工程
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分析化学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
Journal of Innovative Optical Health Sciences
·
2016
·
IF 1.033
Yunfei Zhang,Danqi Li,Xingyu Zhou,Xihui Gao,Shengyuan Zhao,Cong Li
Surface enhanced resonance Raman scattering (SERRS) is a physical phenomenon that occurs when the energy of incident light is close to that of electronic excitation of reporter molecules (RMs) attached on substrates. SERRS has showed great promise in healthcare applications such as tumor diagnosis, image-guided tumor surgery and real-time evaluation of therapeutic response due to its ultra-sensitivity, manipulating convenience and easy accessibility. As the most widely used organic near-infrared (NIR) fluorophore, heptamethine cyanines possess the electronic excitation energy that is close to the plasmon absorption energy of the gold nano-scaffolds, which results in the extraordinary enhancement of the SERRS signal. However, the effect of heptamethine cyanine structure and the gold nanoparticle morphology to the SERRS intensity are barely investigated. This work developed a series of SERRS nanoprobes in which two heptamethine cyanine derivatives (IR783 and IR780) were used as the RM and three gold nanoparticles (nanorod, nanosphere and nanostar) were used as the substrates. Interestingly, even though IR780 and IR783 possess very similar chemical structure, SERRS signal produced by IR780 was determined as 14 times higher than that of IR783 when the RM concentration was 6.5 × 10 −6 M. In contrast, less than 4.0 fold SERRS signal intensity increase was measured by changing the substrate morphologies. Above experimental results indicate that finely tuning the chemical structure of the heptamethine cyanine could be a feasible way to develop robust SERRS probes to visualize tumor or guide tumor resection with high sensitivity and target to background ratio.
研究领域:
生物医学工程
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分子影像学
·
纳米医学
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产品关联
DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Zufeng Qiu,Kexin Yang,Zijian Huang,Hongsheng Zhao,Zhiwei Lin,Qin Kuang,Zhaoxiong Xie
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as a superior technique for detecting small molecules, owing to the strategic utilization of inorganic nanomaterial matrices. Despite the impressive capabilities of various novel matrices, the underlying interaction mechanisms between inorganic matrices and analytes remain largely unexplored. In this study, we synthesized Co 3 O 4 nanocubes, Co(OH) 2 nanosheets, Co 3 O 4 /Co(OH) 2 heterojunctions, and Co 3 O 4 +Co(OH) 2 composites via a facile one-pot method. Amino acids were selected as model analytes for performance evaluation. Notably, the Co 3 O 4 /Co(OH) 2 heterojunctions significantly enhanced signal intensity and lowered detection limits to the parts-per-billion (ppb) level, outperforming Co 3 O 4 nanocubes, Co(OH) 2 nanosheets, and Co 3 O 4 +Co(OH) 2 composites. The detection results for environmental pollutants, especially in solutions containing real samples, highlight the outstanding performance of the heterojunction material as a matrix. Further characterization indicates that the formation of the heterojunction enhances nanoparticle dispersion and promotes the separation of photogenerated electron-hole pairs. Consequently, more photogenerated charge carriers can be transferred to the target analytes, facilitating their charging ability and ultimately enhancing signal intensity in MALDI-TOF MS. These results clearly demonstrate that the enhanced photocatalytic performance directly drives the improvement of MALDI-TOF MS performance, excluding interference from other factors. Thus, this study successfully combines photocatalytic mechanisms with MALDI-TOF MS mechanisms, providing new insights into the enhancement of MALDI-TOF MS performance. Additionally, it offers a new detection method for environmental pollutants.
研究领域:
分析化学
·
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）