ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jiyao Wang,Ruina Zhang,Shihao Li,Binghang Du,Xiaohao Wang,Kai Ni
In Ion Mobility Spectrometry (IMS), ion gates are essential for controlling ion flow, significantly impacting detection sensitivity and resolution. Despite various optimization methods, a clear approach is needed to define the performance limits of ion gates. This study proposes a linear relationship between peak intensity (Ip) and resolving power (Rp) at low ion quantities, introducing it as a reference for evaluating the optimization level. The Tri-State gate (TSG) enables the accurate study of this Ip–Rp relationship, but ion leakage of TSG disrupts control over narrow ion swarms, thereby limiting Rp. After the causes of ion leakage were examined, a preshaping method that removes the gate-opening phase was proposed to precisely control ion injection. This method leverages ion leakage to improve Rp while ensuring the linearity between Ip and Rp. The preserved linearity means avoiding excessive ion loss and shape distortion, ultimately leading to optimal resolving power. Simulations and experiments demonstrate that precise voltage adjustments effectively minimize ion leakage, enhancing resolving power by 50% (reaching a maximum of 106), while the corresponding decrease in signal intensity follows the Ip–Rp linear relationship. This approach expands the accessible range of Ip–Rp combinations without altering their fundamental relationship, reduces discrimination, and establishes a new evaluation method for optimizing ion gate performance in IMS.
研究领域:
分析化学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MEASUREMENT SCIENCE and TECHNOLOGY
·
2013
·Vol. 24
·期 8
·085010
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IF 1.435
Shi Qun Hua,Ying Luo
Luminescent photoelastic coating (LPC) methods are used to measure the full-field shear strain and its principal direction. These methods apply a liquid LPC onto the surfaces of the structures to be measured. To correct the effects of coating thickness non-uniformity on the quantitative shear strain measurements, a non-contact method is proposed for coating thickness measurements. The proposed method establishes a linear calibration curve between the thickness and the luminescent signal intensity of the test coating. The results show that the linear calibration curve of the intensity and the thickness of the LPC can be obtained by incorporating an appropriate concentration of the luminescent dye into the photoelastic coating. The correlation coefficient of this linear calibration curve is more than 0.999.
研究领域:
力学
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材料科学
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DOI
高影响因子
Scientific Reports
·
2015
·Vol. 5
·期 1
·1-10
·
IF 5.578
Zhang Youlin,Li Xiaokun,Xue Bin,Kong Xianggui,Liu Xiaomin,Tu Langping,Chang Yulei
Silica-coated SERS tags have been attracting greater attention in recent years. However, the reported methods to synthesize these tags are tedious and often subjected to the limited signal intensity. Here, we report a facile and general method to prepare the silica-coated Ag SERS tags with the enhanced signal intensity by no introducing the primers. This approach mainly depends on the colloidal stability of the Ag NPs in alcohol solution. By decreasing the concentration of salt in Ag NP solution, the citrate-stabilized Ag NPs can be well dispersed in alcohol solution. Based on this, the Ag SERS tags can be directly coated with thickness-controlled and homogeneous silica shells. This approach is highly reproducible for silica shell growth and signal intensity, not depending on the properties of Raman molecules, proved by 7 kinds of the Raman molecules. Moreover, this kind of SERS tags coated with silica hold the stronger SERS signals than the traditional method due to no interference from the priming molecules.
研究领域:
材料科学
·
分析化学
✓ 已验证
DOI
PubMed
高影响因子
Cancer Nanotechnology
·
2024
·Vol. 15
·期 1
·1-21
·
IF 4.5
Huang Yilong,Shen Shasha,Xiao Jie,Luo Cici,Ma Jiyao,Huang Xin,Qi Tianfu,Gao Chao,Li Guiyun,Li Fan,He Bo,Chen Bingdi,Han Dan
Background Mesothelin (MSLN) has been implicated in cancer migration, invasion, and prognosis, making it a potential tumor marker. However, the precise role of MSLN in the migration and invasion of malignant pleural mesothelioma (MPM) remains elusive, and effective noninvasive methods for assessing MSLN status are currently lacking. In this study, we focused on MSLN expression and elucidated the underlying mechanisms by which MSLN regulates migration and invasion in MPM. Building upon this knowledge, we developed an MRI nanoprobe that targets MSLN to assess its status in vitro and in vivo by comparing T2 signal intensity and T2 values on magnetic resonance imaging examinations. This nanoprobe combines the anatomical information obtained from MRI with biological information obtained from MSLN for comprehensive evaluation of MPM. Results Notably, we observed that MSLN expression in the epithelial type of MPM was higher and increased continuously with tumor growth than that in other types. In addition, MSLN upregulation promoted N-cadherin, matrix metalloproteinase-7, and MMP9 expression and resulted in higher migration/invasion ability and shorter survival. We synthesized MSLN-targeted nanoprobes (Fe 3 O 4 @SiO 2 -PEG-MSLN, FSPM) to assess MSLN expression by comparing the T2 signal intensity and T2 value of different cell lines and mice after 14, 28, and 42 days of modeling. Remarkably, MSLN-targeted nanoprobes demonstrated excellent targeting capabilities. In vitro studies revealed a pronounced reduction in T2 signal intensity and T2 values of the epithelial type as the probe concentration increased. In addition, in vivo experiments demonstrated a gradual decline in these parameters over time, particularly in the epithelial type as compared to the biphasic type, corresponding to the dynamic expression patterns of MSLN during different growth stages. Conclusion Our comprehensive research succeeded in confirming the regulatory mechanisms by which MSLN influences migration and invasion. Moreover, we introduced a promising method for monitoring MSLN expression that may help in facilitating the early detection, histological subtype identification, and assessment of migration, invasion, and prognosis in MPM.
研究领域:
医学影像
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癌症生物学
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分子生物学
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纳米技术
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产品关联
DOI
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2020
·Vol. 412
·期 12
·2731-2741
·
IF 3.637
Chen Xuejing,Zhou Xuesi,He Qinghua,He Yonghong,Guan Tian,Feng Guangxia,Wang Bei,Xie Luyuan,Ji Yanhong
A one-step synthesis using the reversed-phase suspension polymerization method and ultraviolet light curing is proposed for preparing the Raman-encoded suspension array (SA). The encoded microcarriers are prepared by doping the Raman reporter molecules into an aqueous phase, and then dispersing the aqueous phase in an oil phase and curing by ultraviolet light irradiation. The multiplexed biomolecule detection and various concentration experiments confirm the qualitative and quantitative analysis capabilities of the Raman-encoded SA with a limit of detection of 52.68 pM. The narrow bandwidth of the Raman spectrum can achieve a large number of codes in the available spectral range and the independence between the encoding channel and the fluorescent label channel provides the encoding method with high accuracy. This preparation method is simple and easy to operate, low in cost, and high in efficiency. A large number of hydrogel-based encoding microbeads could be quickly obtained with good biocompatibility. Most importantly, concentrating plenty of Raman reporter molecules inside the microbeads increases the signal intensity and means the molecular assembly is not limited by the functional groups; thus, the types of materials available for Raman encoding method are expanded. Furthermore, the signal intensity–related encoding method is verified by doping different proportions of Raman reporter molecules with our proposed synthesis method, which further increases the detection throughput of Raman-encoded SA.
Graphical Abstract
研究领域:
材料科学
·
分析化学
·
生物技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6.8
Zhongyuan Cai,Lingling Jiang,Yingzi Cao,Shengxiang Fu,Shasha Wang,Yuting Jiang,Haojie Gu,Na Li,Xiaomin Fu,Shimin Tang,Jiang Zhu,Weidong Cao,Lei Zhong,Zhuzhong Cheng,Chunchao Xia,Su Lui,Bin Song,Qiyong Gong,Hua Ai
Effective vascular and hepatic enhancement and better safety are the key drivers for exploring gadolinium-free hepatobiliary contrast agents. Herein, a facile strategy proposes that the high lipophilicity may be favorable to enhancing sequentially vascular and hepatobiliary signal intensity based on the structure–activity relationship that both hepatic uptake and interaction with serum albumins partly depend on lipophilicity. Therefore, 11 newly synthesized derivatives of manganese o-phenylenediamine-N,N,N′,N′-tetraacetic acid (MnLs) were evaluated as vascular and hepatobiliary agents. The maximum signal intensities of the heart, liver, and kidneys were strongly correlated with log P, a key indicator of lipophilicity. The most lipophilic agent, MnL6, showed favorable relaxivity when binding with serum albumin, good vascular enhancement, rapid excretion, and reliable hepatobiliary phases comparable to a classic hepatobiliary agent, gadolinium ethoxybenzyl diethylenetriamine pentaacetic acid (Gd-EOB-DTPA) for in vivo liver tumor imaging. Inhibition experiments confirmed the hepatic targeting of MnL6 is mediated by organic anion-transporting polypeptides.
研究领域:
医学成像
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药物化学
·
放射学
✓ 已验证
DOI
PubMed
高影响因子
Journal of Materials Chemistry A
·
2024
·Vol. 12
·期 28
·17151-17157
·
IF 10.7
Zhenli Sun,Ning Wang,Yiyan Zhang,Xunlong Ji,Zijin Hong,Dan Xie,Wentao Zhang,Wenjing Liu,Jingjing Du
Surface-enhanced Raman scattering (SERS) is a pivotal analytical technique in sensing, yet it grapples with challenges such as reproducibility, substrate stability, and hotspot uniformity. The use of highly effective external field nanocomposites facilitates the dynamic manipulation of “hotspots”—the nanoscale gaps between metal nanoparticles—which is critical for SERS enhancement. The gap between 4-mercaptopyridine-modified (4-MPY) Fe3O4@SiO2@Au nanocomposites (FA) can be precisely reduced by applying an external magnetic field, resulting in up to a 30-fold increase in SERS signal intensity and an 8–39 times increase in the enhancement factor (EF). This approach markedly enhances SERS signal uniformity, demonstrated by the relative standard deviation of the EF dropping from 59.15–9.44% to 28.70–1.14%. By systematically studying the correlations between the SERS EF and the external-magnetic-field strength, hotspot density, and probe molecular density, sensitive magnetic, SERS platforms are established. The optimal hotspots occur in the magnetic field of 80 to 120 mT among the FA-4MPY nanocomposites, as validated by COMSOL finite-element analysis simulations. These findings offer a nuanced understanding of magnetic SERS hotspot tuning, paving the way for precise and improved molecular sensing.
研究领域:
分析化学
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纳米技术
·
材料科学
·
光谱学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）