SENSORS AND ACTUATORS B-CHEMICAL
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2018
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IF 5.667
Yachao Zhang,Yan Gao,Xueli Zhang,Huiming Wang,Tian Xia,Congcong Bian,Shuang Liang,Xiaozhi Tang,Xia Wang
The amplification of the electrochemical signal is crucial to improving the sensing performance of an immunosensor. In this study, a signal amplification strategy based on the co-catalysis of horseradish peroxidase (HRP) and nanoporous gold (NPG) was used to fabricate an electrochemical immunosensor for the determination of Hepatitis B e antigen (HBeAg). For the resulting immunosensor, HRP catalyzed catechol and thus served as a signal label of secondary antibody. NPG with unique properties was selected as a carrier for capture antibody immobilization. Further, NPG had an efficient electrocatalytic activity towards catechol (the substrate of HRP), which thus significantly enhanced the electrochemical signal produced by the HRP-labeled secondary antibody. Based on the co-catalysis of HRP and NPG, the immunosensor exhibited a good linear relationship between peak current density and HBeAg concentration in a wide HBeAg detection range from 1 pg mL −1 to 1 ng mL −1 with a high sensitivity of 23.51 μA mL ng −1 cm −2 as well as a low detection limit of 0.064 pg mL −1 (S/N = 3). Additionally, the immunosensor presented a strong anti-interference capability against human serum, human serum/dopamine, human serum/tryptophan, human serum/NO 2 − , human serum/D-glucose, and human serum/IgG. For the detection of HBeAg in human serum, the concentrations detected by the immunosensor were in good agreement with the standard concentrations that added in the human serum samples. These unique properties potentially make the proposed immunosensor a good choice for HBeAg detection.
研究领域:
生物医学工程
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
BIOELECTROCHEMISTRY
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2022
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IF 5.76
Wenjing Lai,Jiajia Li,Mingzhe Jiang,Chaoyun Ma,Chulei Zhao,Min Wang,Pengli Li,Shijie Yan,Yu Qi,Chenglin Hong
In electrochemical immunoassays, great breakthroughs have been made in ultrasensitive detection of tumor markers by amplifying signals with coreaction accelerators. Herein, carbon nitrides with nitrogen vacancies (CNNVs) are proposed as emitter, due to the introduction of nitrogen vacancies this emitter has better ECL efficiency, the phenomena of interface electron transfer and electrode passivation are improved. At the same time, it can also promote the electrochemical reduction of coreactant, making it an attractive and potential emitter. The electrode was modified with Ag-Ti 3 C 2 MXene. It not only accelerates electron transfer and increases the effective working area of the electrode, but also acts as a coreaction accelerator to promote the electrochemical reduction of the coreactant K 2 S 2 O 8 , resulting in a strong ECL signal. The immunosensor showed a good linear relationship in the range of 10 −5 -100 ng/mL with a detection limit of 0.36 fg/mL. In addition, the excellent properties of good specificity and ultra-high stability provide an effective method for ultra-sensitive immunoassays.
研究领域:
生物分析化学
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肿瘤学
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免疫学
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纳米生物技术
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分子诊断
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转化医学
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2017
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IF 7.78
Chao Liu,Jing Dong,Geoffrey I.N. Waterhouse,Ziqiang Cheng,Shiyun Ai
A sensitive sandwich-type electrochemical immunosensor was developed for the detection of avian leukosis virus subgroup J (ALV-J), which benefitted from multiple signal amplification involving graphene-perylene-3,4,9,10-tetracarboxylic acid nanocomposites (GR-PTCA), nanocellulose-Au NP composites (NC-Au) and the alkaline phosphatase (ALP) catalytic reaction . GR-PTCA nanocomposites on glassy carbon electrodes served as the immunosensor platform. Due to their excellent electrical conductivity and abundant polycarboxylic sites, the GR-PTCA nanocomposites allowed fast electron transfer and good immobilization of primary antibodies, thereby affording a strong immunosensor signal in the presence of ALV-J. The detected signal could be further amplified by the introduction of NC-Au composites as a carrier of secondary antibodies (Ab 2 ) and by harnessing the catalytic properties of Au and ALP. Under optimized testing conditions, the electrochemical immunosensor displayed excellent analytical performance for the detection of ALV-J, showing a linear current response from 10 2.08 to 10 4.0 TCID 50 /mL (TCID 50 : 50% tissue culture infective dose) with a low detection limit of 10 1.98 TCID 50 /mL (S/N = 3). In addition to high sensitivity, the immunosensor showed very good selectivity, reproducibility and operational stability, demonstrating potential application for the quantitative detection of ALV-J in clinical diagnosis.
研究领域:
免疫学
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生物传感器
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分子诊断
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兽医病毒学
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纳米医学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2017
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IF 5.401
Huiqiang Wang,Zhanfang Ma
A new strategy for signal amplification was introduced in a label-free amperometric immunoassay platform. Improvements in the signal occurred following the oxidation of glucose by glucose oxidase and catalysis by polythionine of the produced H 2 O 2 from the previous oxidation reaction. In addition, a multifunctional conductive hydrogel, polypyrrole-polythionine-gold with glucose oxidase , was synthesised with a one-pot method using pyrrole and thionine as monomers, ammonium persulfate and HAuCl 4 as co-oxidant agents, and glucose oxidase as the doping agent. Combined with the hydrogel, a label-free amperometric immunoassay for the tumour marker neuron-specific enolase (NSE) was obtained. If operated at 0 V (vs. Ag/AgCl), the sensor exhibited a wide liner range from 100 ng mL −1 to 1 pg mL −1 and ultralow detection limit of 0.65 pg mL −1 (S/N = 3). The presented method was further used for the analysis of human serum samples and showed good consistency with detection results from an electrochemiluminescence immunoassay.
研究领域:
肿瘤学
·
免疫学
·
生物传感器
·
分子诊断
·
转化医学
·
临床检验医学
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纳米生物技术
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）