ACS Sensors
·
2016
·
IF 0
Hui Zhang,Yunsheng Xia
In this study, we have reported a new colorimetric platform for sensitive and selective sensing of Hg2+ using near-infrared (NIR) plasmonic Cu2-xSe nanoparticles (NPs) as reporters. Because of ultrahigh affinity between Hg2+ and Se2–, the added Hg2+ can react with Cu2-xSe NPs and exchange their Cu+/Cu2+, yielding a HgSe layer around the host NPs. Accordingly, the absorption profiles of the Cu2-xSe NPs are modulated substantially: The absorbance at 400–600 nm is increased, and the NIR localized surface plasmon resonance dramatically decreases with a more than 150 nm bathochromic shift. Thus, the system possesses triple signal responses, namely, ratiometry, wavelength, and intensity, to the analytes simultaneously. Such uniquely multiple signal output not only provides more choices for the quantification, but also enhances the reliability in the analyte detection. By rationally choosing poly(allylamine hydrochloride) as the NP template, Hg2+ ions can be determined as ranging from 0–800 nM. The detection limit is as low as 2.7 nM, which is nearly 4 times lower than the limit value (10 nM) defined by the U.S. Environmental Protection Agency for drinking water. Other heavy/transition metal ions, such as Cu2+, Ag+, Pb2+, Cd2+, Ni2+, Co2+, Mn2+, Zn2+, Cr3+, Fe2+, and FeF63–, do not interfere with the sensing. Especially, Hg2+ contents can be quantified, even if their concentrations are as low 10 nM in tap water and common environmental water samples. Due to favorable analytical performance, the proposed Cu2-xSe NPs based system has potential applications in monitoring trace Hg2+ in various real samples, even in drinking water.
研究领域:
分析化学
·
环境科学
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产品关联
DOI
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Yanjie Chen,Shupei Zhang,Yitian Huang,Liang Lv,Hong Dai,Yanyu Lin
Herein, a photothermal triggered multi-signal readout (MSR) system was innovatively established with great convenience for low-cost and sensitive point-of-care testing (POCT). In this sensing system, an intelligent multi-signal readout interface (MSRI) with multidimensional response-ability to thermal stimulus was developed and utilized as a sensing element. A bio-bar-code photothermal probe peptides@H 2 N-HCNTs acted as a target associated photothermal agent anchored on MSRI via competitive reaction. The multi-signal assay of target was realized under the driven of 808 nm laser, photo-to-thermal conversion effect of photothermal probe caused dramatically thermal energy increase on MSRI. As a result, the competitive recognition events were translated into several detectable signals on MSRI, including a local temperature elevation, a visual color change from blue to green as well as weight loss on MSRI, all of these signals were proportional to the target concentration. This assay has been successfully applied in field work for detecting zearalenone (ZEN), a common mycotoxin in grain food, with linear ranger from 10 −7 ng/mL to 10 −1 ng/mL and detection limits of 1.06 × 10 −7 ng/mL. Combination of the different signal principles was expected to result in more reliable and precise results. Accordingly, this creatively designed MSR-system not only provided a platform for sensitive monitor of mycotoxin but also offered new method for reliable and affordable personal assays in daily life and low-resource setting.
研究领域:
分析化学
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食品安全
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Mingcong Rong,Yi Huang,Xiaoting Zhuang,Yingming Ma,Haijiao Xie,Yanfang Wu,Li Niu
Tetracyclines (TCs) are the most common antibiotic drugs in the world. However, due to the overuse and difficulty of degradation, TCs pollution is becoming a global threat to aquatic and terrestrial biodiversity. Current methods for detecting TCs mainly rely on fluorescent probes or nanomaterials with enzyme-mimicking catalytic activities , which only provide a single signaling mode and are vulnerable to experimental factors. Herein, this study reports the development of an AND logic-gate-based sensing platform for TCs with fluorescent and colorimetric dual-signal readouts using Au@MnO 2 nanoparticles that could be mutually verified for the selective sensing of TCs. It was found that Au@MnO 2 nanoparticles could oxidize TC into its oxidation state (oxTC), resulting in significant green fluorescence emission . Meanwhile, the MnO 2 shell was etched away, and the released Au core nanoparticles gradually aggregated, with a color change from brown to light blue. As such, we established the fluorescence “turn-on” and colorimetric quantitative relationships towards TC with detection limits of 21.9 and 83.3 nM, respectively. Furthermore, Au@MnO 2 -based analytical paper devices were prepared and demonstrated successfully for the fluorescent and colorimetric dual-signal readout detection of TC in real samples. This paper sheds light on the on-site detection of tetracycline using paper-based analytical devices and smartphones.
研究领域:
分析化学
·
环境科学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2022
·
IF 10.383
Lihua Zhi,Shengya Zhang,Min Li,Jibing Tu,Xiaoquan Lu
The exploration of new strategies for portable detection of mercury ions with high sensitivity and selectivity is of great value for biochemical and environmental analyses. Herein, a straightforward, convenient, label-free, and portable sensing platform based on a Au nanoparticle (NP)-decorated WO3 hollow nanoflower was constructed for the sensitive and selective detection of Hg(II) with a pressure, temperature, and colorimetric triple-signal readout. The resulting Au/WO3 hollow nanoflowers (Au/WO3 HNFs) could efficaciously impede the aggregation of Au NPs, thus significantly improving their catalytic activity and stability. The sensing mechanism of this new strategy using pressure as a signal readout was based on the mercury-triggered catalase mimetic activity of Au/WO3 HNFs. In the presence of the model analyte Hg(II), H2O2 in the detection system was decomposed to O2 fleetly, resulting in a detectable pressure signal. Accordingly, the quantification of Hg(II) was facilely realized based on the pressure changes, and the detection limit could reach as low as 0.224 nM. In addition, colorimetric and photothermal detection of Hg(II) using the Au/WO3 HNFs based on their mercury-stimulated peroxidase mimetic activity was also investigated, and the detection limits were calculated to be 78 nM and 0.22 μM for colorimetric and photothermal methods, respectively. Hence, this nanosensor can even achieve multimode determination of Hg(II) with the concept of point-of-care testing (POCT). Furthermore, the proposed multimode sensing platform also displayed satisfactory sensing performance for the Hg(II) assay in actual water samples. This promising strategy may provide novel insights on the fabrication of a multimode POCT platform for sensitive, selective, and accurate detection of heavy metal ions.
研究领域:
分析化学
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环境科学
·
生物医学工程
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Mengyuan Yin,Yaxing Zhang,Hanxue Liang,Chang Liu,Yanhui Bi,Juanjuan Sun,Weiwei Guo
Stimuli-responsive DNA hydrogels have shown great potential in sensing applications due to their attractive properties such as programmable target responsiveness, excellent biocompatibility, and biodegradability. In contrast to the extensively developed DNA hydrogel sensing systems based on the stimuli-responsive hydrogel-to-solution phase transition of the hydrogel matrix, the quantitative sensing application of DNA hydrogels exhibiting smart shape deformations has rarely been explored. Moreover, bulk DNA hydrogel-based sensing systems also suffer from high material cost and slow response. Herein, free-standing bilayer polyacrylamide/DNA hybrid hydrogel films with programmable responsive properties directed by the sequence of functional DNA units have been constructed. Compared with bulk DNA hydrogels, these DNA hydrogel films with a thickness at the micrometer scale not only greatly reduce the consumption of DNA materials but also facilitate the mass transfer of biomacromolecular substances within the hydrogel network, thus favoring their sensing applications. Therefore, a target-responsive smart DNA hydrogel film-based sensor system is further demonstrated based on the large amplitude macroscopic shape deformation of the film as a visual signal readout. As a proof of concept, Pb2+ or UO22+ ion-responsive DNA units were introduced into the active layer of the bilayer hydrogel films. In the presence of Pb2+ or UO22+ ions, the occurrence of a cleavage reaction within the DNA units leads to the release of DNA segments from the hydrogel film, inducing a dramatic shape deformation of the film, and thus sensing of Pb2+ or UO22+ ions with high specificity is achieved based on measuring the bending angle changes of these smart free-standing films. These smart DNA hydrogel film sensors with target-programmable responsiveness, simple operation, and ease of storage may hold promise for future rapid on-site testing applications.
研究领域:
生物化学
·
材料科学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Yuye Li,Dong Liu,Shuyun Meng,Jiayi Zhang,Libo Li,Tianyan You
Ferrocene (Fc) is a common quencher of Ru(bpy)32+ luminescence. However, interactions between Fc and Ru(bpy)32+ can be extremely complicated. In this work, we reported the first use of Fc to regulate the electrochemiluminescence (ECL) of Ru(bpy)32+ by tuning the length of the DNA sequence between Fc and the luminophore of nitrogen-doped graphene quantum dots-Ru(bpy)32+-doped silica nanoparticles (SiO2@Ru-NGQDs). The ECL of SiO2@Ru-NGQDs was depressed when the distance between Ru(bpy)32+ and Fc was less than 8 nm; a stronger ECL was observed when the distance was more than 12 nm. The switching of the ECL of Ru(bpy)32+ by Fc was attributed to the electron transfer mechanism, in which Fc participated in the redox of Ru(bpy)32+ for “signal-off” ECL; this favored electron transfer at the electrode fabricated with an Fc-labeled aptamer (Fc-apt) and SiO2@Ru-NGQDs for “signal-on” ECL depending on the length of the DNA sequence. Here, a dual-signal readout aptasensor for aflatoxin B1 (AFB1) detection was developed via the enhanced ECL of SiO2@Ru-NGQDs by Fc-apt. The redox currents of Fc and the ECL of Ru(bpy)32+ were simultaneously collected as yardsticks, and both decreased with higher concentrations of AFB1. The aptasensor allowed linear ranges of 3 × 10–5 to 1 × 102 ng mL–1 for ECL mode and 1 × 10–3 to 3 × 103 ng mL–1 for electrochemical mode. Our work provides insight into the interactions between Fc and Ru(bpy)32+. The dual-signal readout strategy is a potential platform for the versatile design of aptasensors.
研究领域:
分析化学
·
生物传感
✓ 已验证
DOI
PubMed
高影响因子
ANALYST
·
2013
·Vol. 138
·期 21
·6551-6557
·
IF 3.969
Bangda Yin,Jianhui Deng,Xue Peng,Qian Long,Jiangna Zhao,Qiujun Lu,Qiong Chen,Haitao Li,Hao Tang,Youyu Zhang,Shouzhuo Yao
High quality carbon dots (C-dots) with down- and up-conversion fluorescence have been synthesized through low-temperature carbonization using sweet pepper as the carbon source. The C-dots with a quantum yield (QY) of 19.3% exhibit superior photophysical properties, for example, narrow and symmetric emission spectra, large stock shifts, resistance to photobleaching, and excitation-dependent fluorescence behavior. The excellent C-dots serve as useful fluorescent probes for hypochlorite (ClO−) detection by both down- and up-conversion fluorescence. Two consecutive linear ranges allow a wide determination of ClO− concentrations with a low detection limit of 0.05 μmol L−1 and 0.06 μmol L−1 (S/N = 3) for down- and up-conversion fluorescence measurements, respectively. The proposed detection method is advantageous because it is simple, sensitive, dual-signalling model and low-cost and has potential extensive applications in environmental and biological assays.
研究领域:
分析化学
·
纳米材料
·
环境监测
·
生物传感
·
荧光探针
·
分子诊断
✓ 已验证
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 3
·1-10
·
IF 6.232
Zhu Wanying,Zhou Ying,Tao Mengdan,Yan Xiaoqiang,Liu Yan,Zhou Xuemin
A novel electrochemical and fluorescence dual-signal assay was developed for the determination of hydrogen peroxide (H 2 O 2 ) based on Fe 3 O 4 @MnO 2 and N-doped carbon dots (NCDs). Fe 3 O 4 @MnO 2 was not only applied as the recognizer for H 2 O 2 but also served as the fluorescence quencher and electrochemical enhancer. This permits the dual-signal readout of the analytical system. In the absence of H 2 O 2 , the NCDs were quenched by Fe 3 O 4 @MnO 2 , and the oxidation of the electrochemical probe ferrocene (Fc) was catalyzed by Fe 3 O 4 @MnO 2 . In the presence of H 2 O 2 , MnO 2 was reduced to Mn 2+ , leading to the fluorescence recovery of NCDs and the reduction in the oxidation signal of Fc. By combining the electrochemical method and the fluorescence assay, more comprehensive and valuable information for H 2 O 2 determination was provided to meet different analytical demands. The method exhibits good repeatability and selectivity with a detection limit of 1.0 μM for the fluorescence assay and 0.6 μM for the electrochemical method. The proposed approach holds great potential for probing released targets from living cells.
Graphical abstract
研究领域:
分析化学
·
电化学
·
荧光光谱学
·
纳米材料
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2025
·
IF 7.7
Jiarong Lin,Ye Deng,Yue Lin,Fang Luo,Zuquan Weng,Jian Wang,Cuiying Lin,Bin Qiu,Zhenyu Lin
In this study, we present the innovative design and comprehensive evaluation of a novel point-of-care testing (POCT) methodology for the rapid and accurate detection of T-2 toxin, a potent mycotoxin with significant implications for food safety and human health. The cornerstone of this approach lies in the integration of a copper-based conductive metal-organic framework (Cu 3 (HHTP) 2 ) with a target-responsive DNA hydrogel system, creating a dual-signal readout mechanism that significantly enhances detection sensitivity and specificity. Specifically, the hydrogel covers the material's surface metal active sites, which become exposed upon target addition due to hydrogel collapse. The released Cu 3 (HHTP) 2 can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine, resulting in a colorimetric and temperature change in the solution. This dual-mode detection strategy enables both qualitative assessment through direct visual inspection of color change and quantitative analysis by monitoring the solution temperature variation post laser irradiation with a thermometer. Under the optimized conditions, the detection system demonstrates a wide range spanning from 5 to 200 ng/mL, with a detection limit of 1.67 ng/mL. This method has been successfully demonstrated through the analysis of real-world samples, yielding encouraging results that underscore its reliability and effectiveness. The proposed dual-signal approach boasts advantages such as straightforward operational procedures, unambiguous signal outputs, and robust practicality, making it an attractive option for mycotoxin detection in resource-constrained settings and developing regions.
研究领域:
食品安全
·
生物传感
·
毒理学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Xuechi Yin,Yanli Tian,Yaqian Chen,Leina Dou,Jing Guo,Jingxin Hou,Yanru Wang,Jing Sun,Jianlong Wang,Daohong Zhang
The single-signal colorimetric immunochromatography assay (ICA) is becoming increasing inadequate in dealing with current detection requirement, e.g., more flexible, accurate, and sensitive detection . Herein, by introducing tannic acid (TA) to exfoliate molybdenum disulfide (MoS 2 ) nanosheets and selecting anisotropic gold nanorods (AuNRs) as the nanocarriers , a dual-signal nanocomposite of TA/MoS 2 @AuNRs was steadily constructed. The nanocomposite possesses excellent signal transmission and photothermal properties because of the high NIR absorption cross-section and signal intensity of AuNRs as well as strong photothermal conversion efficiency of MoS 2 . The TA/MoS 2 @AuNRs were used to prepare immunoprobes and then applied in the ICA. With 2-nitrobenzaldehyde semicarbazone (2-NPSEM) as the model analyte , the obtained competitive photothermal/colorimetric dual-signal readout ICA (PCICA) can monitor NPSEM colorimetrically and photothermally with a calculated sensitivity of 0.267 and 0.180 ng mL −1 , respectively. Furthermore, the application feasibility of the immunoassay was further evaluated by measuring SEM in the honey sample with satisfactory recoveries ranging from 83.35% to 107.14% and coefficient of variation (CV) ranging from 0.12% to 7.63%. As a result, by introducing TA to exfoliate MoS 2 nanosheet, this work has proposed a more stable photothermal/colorimetric platform and provide an invigorating pathway for the appliance of MoS 2 -related photothermal materials in point-of-care immunoassays.
研究领域:
分析化学
·
纳米技术
·
生物医学工程
·
食品科学
✓ 已验证
产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2022
·
IF 16.744
Feng Hong,Liangqiong Ren,Yiping Chen
In this work, we report a fluorescent and colorimetric dual readout bioassay for deoxynivalenol detection to ensure public food safety. A multifunctional fluorescent and colorimetric signal probe was constructed to develop the bioassay for deoxynivalenol detection based on the characteristics of Zr-MOF (PCN-224; i.e., high porosity, a large surface area, and excellent fluorescence quenching performance). Platinum nanoparticles (Pt NPs) were grown in situ outside, while glucose oxidase was stored inside (PPG). Glucose oxidase and Pt NPs are both enriched by Zr-MOF, achieving the first signal amplification, and cascade catalysis by the glucose-TMB system for colorimetric signal readout leads to the second signal amplification. PPG was quenched through the spontaneous formation of Zr O P bonds with fluorescent DNA to achieve a highly sensitive fluorescent readout. The self-supplied H 2 O 2 in the colorimetric detection further improved the self-accuracy of the dual-mode analysis strategy (the accuracy of independent signal readout modes from two different signal transduction mechanisms). This multifunctional PPG probe avoids the time-consuming assembly of different probes and reduces error accumulation from uncontrollable factors in complex experimental procedures. It allows for the sensitive and accurate detection of deoxynivalenol with excellent limits of detection (5.3 pg/mL for the fluorescent assay and 34 pg/mL for the colorimetric method). Overall, this work demonstrates a viable approach for the construction of a dual-signal biosensing platform based on multifunctional MOF probes for biological analysis.
研究领域:
分析化学
·
生物化学
·
食品安全
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Fang Luo,Linxiu Zhan,Ye Deng,Kun Qiao,Nan Pan,Zuquan Weng,Cuiying Lin,Bin Qiu,Zhenyu Lin
Point-of-care testing (POCT) has experienced rapid development owing to its advantages of rapid testing, low cost and strong operability, making it indispensable for analyte detection in outdoor or rural areas. In this study, we propose a novel method for the detection of aflatoxin B 1 (AFB 1 ) using a dual-signal readout approach within a unified system. This method employs dual channel modes, namely visual fluorescence and weight measurements, as the signal readouts. Specifically, a pressure-sensitive material is utilized as a visual fluorescent agent, its signal can be quenched in the presence of high oxygen pressure . Additionally, an electronic balance, commonly used for weight measurement, is adopted as another signal device, where the signal is generated through the catalytic decomposition of H 2 O 2 by platinum nanoparticles . The experimental results demonstrate that the proposed device enables accurate AFB 1 detection within the concentration range of 1.5–32 μg mL −1 , with a detection limit of 0.47 μg mL −1 . Moreover, this method has been successfully applied for practical AFB 1 detection with satisfactory results. Notably, this study pioneers the use of a pressure-sensitive material as a visual signal in POCT. By addressing the limitations of single-signal readout approaches, our method fulfills requirements of intuitiveness, sensitivity, quantitative analysis and reusability.
研究领域:
分析化学
·
生物技术
·
食品安全
·
即时检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）