PLoS One
·
2015
·Vol. 10
·期 6
·e0131407
·
IF 3.234
Xinda Lin,Qinhui Zhang,Zhongnan Wu,Yongjun Du
Olfaction is primarily mediated by highly specific olfactory receptors (ORs), a subfamily of which are the pheromone receptors that play a key role in sexual communication and can contribute to reproductive isolation. Here we cloned and identified an olfactory receptor, SlituOR3 (Genbank NO. JN835270), from Spodoptera litura , to be the candidate pheromone receptor. It exhibited male-biased expression in the antennae, where they were localized at the base of sensilla trichoidea. Conserved orthologues of these receptors were found amongst known pheromone receptors within the Lepidoptera, and SlituOR3 were placed amongst a clade of candidate pheromone receptors in a phylogeny tree of insect ORs. SlituOR3 is required for the EAG responses to both Z9E11-14:OAc and Z9E12-14:OAc SlituOR3 showed differential expression in S . litura populations attracted to traps baited with a series of sex pheromone blends composed of different ratios of (9Z,11E)-tetradecadienyl acetate (Z9E11-14:OAc) and (9Z,12E)-tetradecadienyl acetate (Z9E12-14:OAc). The changes in the expression level of SlitOR3 and antennal responses after SlitOR3 silencing suggested that SlitOR3 is required for the sex pheromone signaling. We infer that variation in transcription levels of olfactory receptors may modulate sex pheromone perception in male moths and could affect both of pest control and monitoring efficiency by pheromone application after long time mass trapping with one particular ratio of blend in the field.
研究领域:
昆虫学
·
分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF ASIA-PACIFIC ENTOMOLOGY
·
2021
·Vol. 25
·期 1
·101859
·
IF 1.303
Jianyu Deng,Zhijie Shen,Fumin Wang,Tao Liu,Wenying Hong,Minghua Fang,Linfeng Wo,Sijie Chu
Grapholita molesta (Busck) (the oriental fruit moth; OFM) and Conogethes punctiferalis (Guenée) are both fruit and stem borers with overlapping habitats, occurrences, and outbreak cycles. These two species are in different families and they have completely different sex pheromone components. Here, the effects of the sex pheromone components of C. punctiferalis , ( E )-10-hexadecenal ( E 10-16:Ald) and ( Z )-10-hexadecenal ( Z 10-16:Ald) and their mixture, were evaluated on the sexual communication of OFM by examining electrophysiological (EAG) and behavioral responses. We found that a considerably large amount of E 10-16:Ald or Z 10-16:Ald and a tiny amount of OFM pheromone elicited comparable EAG responses in OFM males, indicating the low antennal olfactory sensitivity of OFM males to the heterospecific pheromone compounds. In two different peach orchard parcels, captures of OFM by their pheromone lures baited in delta traps were increased by at least 1.5 times when OFM pheromone lures were combined with lures that contained 1000 μg of either E 10-16:Ald, Z 10-16:Ald or their mixture. In two other pear orchard parcels, both E 10-16:Ald and Z 10-16:Ald increased captures of OFM in Unitraps in a dose-dependent manner with more than a four-fold increase. Therefore, the enhanced attractiveness of OFM lures by the two interspecific pheromones suggests their potential application to improve mass trapping, population monitoring and mating disruption of OFM.
研究领域:
昆虫学
·
化学生态学
·
农业昆虫学
·
害虫综合治理
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行为生理学
✓ 已验证
DOI
高影响因子
PEST MANAGEMENT SCIENCE
·
2021
·Vol. 78
·期 2
·643-652
·
IF 4.845
Jiyuan Liu,Tong Zhou,Chaoxia Li,Ruichi Li,Xuan Ye,Zhen Tian
BACKGROUND Pheromone-based management is a leading nonpesticidal strategy among integrated pest management options. Improving the potency of pheromone products by adding synergists would be a practical way to popularize pheromone-based management as well as to reduce pesticide use. RESULTS Using reverse chemical ecology, synergists for Grapholita molesta sex pheromone were screened. Combined molecular docking and in vitro binding assay led to the determination of four potentially active odorants showing high affinity to G. molesta pheromone binding protein 2 (GmolPBP2). Thereafter, the high affinity between Codlemone and GmolPBP2 was further verified by exploration of GmolPBP2–Codlemone interactions. As the only sex pheromone synergist validated by both laboratory behavioral tests and field trapping, Codlemone was used to optimize commercial sex attractants currently used in G. molesta control. The recommended formulation [( Z )-8-dodecenyl acetate:( E )-8-dodecenyl acetate:Codlemone = 95:4:10] was found to trap about five to six times more G. molesta adults than the commercial sex attractant [( Z )-8-dodecenyl acetate:( E )-8-dodecenyl acetate: ( Z )-8-dodecenol = 95:4:1]. CONCLUSION Codlemone is an excellent pheromone synergist that can be potentially sensed by GmolPBP2, which can remarkably improve the potency of G. molesta sex attractants. It is believed that the introduction of reverse chemical ecology would increase the chance of discovering pheromone synergists, promoting the development of more efficacious pheromone products that can be used in controlling G. molesta and beyond. © 2021 Society of Chemical Industry.
研究领域:
昆虫学
·
化学生态学
·
害虫管理
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产品关联
DOI
PubMed
高影响因子
PeerJ
·
2019
·
IF 2.353
Hua Yang,Yan-Lin Liu,Yuan-Yuan Tao,Wei Yang,Chun-Ping Yang,Jing Zhang,Li-Zhi Qian,Hao Liu,Zhi-Yong Wang
The bamboo snout beetle Cyrtotrachelus buqueti is a widely distributed wood-boring pest found in China, and its larvae cause significant economic losses because this beetle targets a wide range of host plants. A potential pest management measure of this beetle involves regulating olfactory chemoreceptors. In the process of olfactory recognition, pheromone-binding proteins (PBPs) play an important role. Homology modeling and molecular docking were conducted in this study for the interaction between CbuqPBP1 and dibutyl phthalate to better understand the relationship between PBP structures and their ligands. Site-directed mutagenesis and binding experiments were combined to identify the binding sites of CbuqPBP1 and to explore its ligand-binding mechanism. The 3D structural model of CbuqPBP1 has six a-helices. Five of these a-helices adopt an antiparallel arrangement to form an internal ligand-binding pocket. When docking dibutyl phthalate within the active site of CbuqPBP1, a CH- π interaction between the benzene ring of dibutyl phthalate and Phe69 was observed, and a weak hydrogen bond formed between the ester carbonyl oxygen and His53. Thus, Phe69 and His53 are predicted to be important residues of CbuqPBP1 involved in ligand recognition. Site-directed mutagenesis and fluorescence assays with a His53Ala CbuqPBP1 mutant showed no affinity toward ligands. Mutation of Phe69 only affected binding of CbuqPBP1 to cedar camphor. Thus, His53 (Between α2 and α3) of CbuqPBP1 appears to be a key binding site residue, and Phe69 (Located at α3) is a very important binding site for particular ligand interactions.
研究领域:
生物信息学
·
生化分析
·
昆虫学
✓ 已验证
DOI
PubMed
高影响因子
CURRENT BIOLOGY
·
2023
·
IF 9.2
Liwei Zhang,Huiwen Sun,Ewald Grosse-Wilde,Long Zhang,Bill S. Hansson,Hany K.M. Dweck
In a heterogeneous and changing environment, oviposition site selection strongly affects the survival and fitness of the offspring. 1 , 2 Similarly, competition between larvae affects their prospects. 3 However, little is known about the involvement of pheromones in regulating these processes. 4 , 5 , 6 , 7 , 8 Here, we show that mated females of Drosophila melanogaster prefer to lay eggs on substrates containing extracts of conspecific larvae. After analyzing these extracts chemically, we test each compound in an oviposition assay and find that mated females display a dose-dependent preference to lay eggs on substrates spiked with (Z)-9-octadecenoic acid ethyl ester (OE). This egg-laying preference relies on gustatory receptor Gr32a and tarsal sensory neurons expressing this receptor. The concentration of OE also regulates larval place choice in a dose-dependent manner. Physiologically, OE activates female tarsal Gr32a + neurons. In conclusion, our results reveal a cross-generation communication strategy essential for oviposition site selection and regulation of larval density.
研究领域:
昆虫学
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行为生态学
·
神经生物学
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产品关联
DOI
PubMed
高影响因子
MICROBIAL ECOLOGY
·
2015
·Vol. 70
·期 4
·1012-1023
·
IF 2.973
Xu Letian,Lou Qiaozhe,Cheng Chihang,Lu Min,Sun Jianghua
Bark beetles are the most important mortality agent in coniferous forests, and pheromones play important roles in their management. Dendroctonus valens LeConte was introduced from North America to China and has killed millions of healthy pines there. Trapping with semiochemicals and pheromones was deployed in D. valens management in the last decade, but little is known about the ability of gut bacteria to produce the pheromone. In this study, we analyzed the volatiles in D. valens guts and frass after antibiotic treatment versus control. Then, we isolated and identified the bacteria in D. valens guts and frass, examined verbenone (a multifunctional pheromone of D. valens ) production by 16 gut bacterial isolates from the precursor cis -verbenol at three concentrations, and further compared the cytotoxicities between the cis -verbenol and verbenone to the bacterial isolates. cis -Verbenol was not detected in the frass in the control group, but it was in the antibiotic treatment. The amount of verbenone was significantly suppressed in D. valens guts after antibiotic treatment versus control. Thirteen out of 16 gut bacterial isolates were capable of cis -verbenol to verbenone conversion, and cis -verbenol had stronger cytotoxicities than verbenone to all tested gut bacterial isolates. The bacterial species capable of verbenone production largely exists in D. valens guts and frass, suggesting that gut-associated bacteria may help the bark beetle produce the pheromone verbenone in guts and frass. The bacteria may benefit from the conversion due to the reduced cytotoxicity from the precursor to the beetle pheromone.
研究领域:
昆虫学
·
微生物学
✓ 已验证
DOI
PubMed
高影响因子
ENVIRONMENTAL ENTOMOLOGY
·
2025
·
IF 1.5
Meng Wei,Zhang Wanming,Zhang Dan,Zheng Jingjun,Huang Dechao,Guo Qianshuang,Du Yongjun
The sex pheromone of the oriental armyworm, Mythimna separata (Walker), in China has always been confusing. It was initially considered as cis-11-hexadecenal (Z11-16:Ald) or cis-11-hexadecenyl acetate (Z11-16:Ac) and cis-11-hexadecenol (Z11-16:OH), as well as a mixture of these compounds. However, inconsistent results have frequently occurred using these compounds or mixtures in field trapping. Through gland analysis from individual females and nationwide field experiments, we identified two distinct types: Z11-16:Ald (aldehyde type or A type) and a mixture of Z11-16:Ac and Z11-16:OH (ester type or E type). Electroantennography (EAG) results indicated that both strains of males co-existed in the field. A strain of males had a strong EAG response to Z11-16:Ald but weak responses to Z11-16:Ac and Z11-16:OH, whereas E strain males had strong responses to Z11-16:Ac and Z11-16:OH but a weak response to Z11-16:Ald. Z11-16:OH identified in female E strain inhibited the attraction of A strain males to A type pheromones. Both A and E types of sex pheromone were respectively formulated and field evaluation (2014-2021) across > 20 provinces from southern to northern China showed geographic and generational variations. During overwintering, E strain males were predominantly caught in Hainan, Fujian, and Guangdong provinces, while A strain males were mainly trapped in Guangxi, Anhui, Sichuan, Jiangsu, and Jiangxi provinces. Subsequent generations exhibited mixed populations in most regions. These findings suggest regional adaptation and migration-driven hybridization. The study highlights the necessity of strain-specific pheromone blends for effective monitoring and control. This work resolves long-standing ambiguities in M. separata sex pheromone ecology.
研究领域:
昆虫学
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化学生态学
·
信息素研究
·
种群遗传学
·
农业害虫治理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL ENTOMOLOGY
·
2015
·Vol. 44
·期 3
·724-733
·
IF 1.295
Du Yongjun,Feng Bo,Li Hongguang,Liu Chunming,Zeng Juan,Pan Lieming,Yu Qing
The attractiveness of a series of mixtures of (Z)-7-dodecenyl acetate (Z7-12:Ac), (Z)-9-tetradecenyl acetate (Z9-14:Ac), and (Z)-11-hexadecenyl acetate (Z11-16:Ac), the Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) pheromone, were evaluated in four locations in China. The ternary blend of Z7-12:Ac, Z9-14:Ac, and Z11-16:Ac was the complete pheromone blend for A. ipsilon and the ratio of Z7-12:Ac and Z9-14:Ac was optimal at 3:1. The most attractive ratio of Z11-16:Ac to the other components depended on geographic location. The optimal ratio was 3:1:6 in Yunnan and Shanxi, 3:1:2 in Sichuan and ranged from 3:1:2 to 3:1:12 in Shanghai, which differs significantly from the ratio of 3:1:16 in Japan. The dose of the blend in the pheromone lure influenced attractiveness to male moths and was related to the temperature in the test locations. Attractiveness of sugar–acetic acid-baited and pheromone-baited traps to male and female moths was different before and after the start of flowering of the oilseed rape crop; large numbers of female moths were attracted to sugar–acetic acid traps before flowering but none after flowering had started. This was similar for male moths and there was no synergistic effect when sugar–acetic acid solutions and pheromone were used together. These studies suggest that pheromone trapping based on the blends of three components can be an effective tool to improve the efficiency of monitoring of this pest in China.
研究领域:
昆虫学
·
农业科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Insect Science
·
2015
·Vol. 24
·期 4
·543-558
·
IF 2.144
Qin-Hui Zhang,Zhong-Nan Wu,Jing-Jiang Zhou,Yong-Jun Du
Olfaction is primarily mediated by highly specified olfactory receptors (ORs). Here, we cloned and identified an olfactory receptor, named SlituOR1 (Genbank no. JN835269), from Spodoptera litura and found evidence that it is a candidate pheromone receptor. It exhibited male-biased expression in the antennae, where it was localized at the base of sensilla trichoidea, the antennal sensilla mainly responsive to pheromones in moths. Conserved orthologues of this receptor, found among known pheromone receptors within the Lepidoptera, and SlituOR1 were placed among a clade of candidate pheromone receptors in a phylogeny tree of insect OR gene sequences. SlituOR1 showed differential expression in S. litura populations attracted to traps baited with different ratios of the two sex pheromone components (9 Z ,11 E )-tetradecadienyl acetate ( Z 9 E 11-14:OAc) and (9 Z ,12 E )-tetradecadienyl acetate ( Z 9 E 12-14:OAc). Knocking down of SlituOR1 by RNA interference reduced the electroantennogram (EAG) response to Z 9 E 11-14:OAc, and this result is consistent with the field trapping experiment. We infer that variation in transcription levels of olfactory receptors may modulate sex pheromone perception in male moths and could provide some of the flexibility required to maintain the functionality of communication with females when a population is adapting to a new niche and reproductive isolation becomes an advantage.
研究领域:
昆虫学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CURRENT BIOLOGY
·
2024
·
IF 8.1
Yinliang Wang,Huanhuan Dong,Yafei Qu,Yuxin Zhou,Jianhui Qin,Kebin Li,Chen Luo,Bingzhong Ren,Yazhong Cao,Shuai Zhang,Jiao Yin,Walter S. Leal
Summary Animals have endogenous clocks that regulate their behavior and physiology. These clocks rely on environmental cues (time givers) that appear approximately every 24 h due to the Earth’s rotation; thus, most insects exhibit a circadian rhythm. One notable exception is the scarab beetle, Holotrichia parallela , a severe agricultural pest in China, Japan, South Korea, and India. Females emerge from the soil every other night, reach the canopy of host plants, evert an abdominal gland, and release a pheromone bouquet comprising l -isoleucine methyl ester (LIME) and l -linalool. To determine whether this circa’bi’dian rhythm affects the olfactory system, we aimed to identify H. parallela sex pheromone receptor(s) and study their expression patterns. We cloned 14 odorant receptors (ORs) and attempted de-orphanizing them in the Xenopus oocyte recording system. HparOR14 gave robust responses to LIME and smaller responses to l -linalool. Structural modeling, tissue expression profile, and RNAi treatment followed by physiological and behavioral studies support that HparOR14 is a sex pheromone receptor—the first of its kind discovered in Coleoptera. Examination of the HparOR14 transcript levels throughout the adult’s life showed that on sexually active days, gene expression was significantly higher in the scotophase than in the photophase. Additionally, the HparOR14 expression profile showed a circabidian rhythm synchronized with the previously identified pattern of sex pheromone emission. 48 h of electroantennogram recordings showed that responses to LIME were abolished on non-calling nights. In contrast, responses to the green leaf volatile (Z)-3-henexyl acetate remained almost constant throughout the recording period.
研究领域:
昆虫学
·
分子生物学
·
行为生态学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Liuyang Wang,Pei Guo,Xiaofang Zhang,Yun Duan,Jun Ning,Tao Zhang,Xinling Yang
In moths, sex pheromones are synthesized in pheromone glands (PGs) by a variety of enzymes. Desaturases (DESs) are critical for the introduction of double bonds into pheromones. In Mythimna loreyi, the specific DESs involved in sex pheromone biosynthesis remain unclear. In this study, we identified and characterized 25 putative DESs from the M. loreyi genome. Nineteen of them were expressed in the female PGs, with seven showing significant upregulation in response to pheromone biosynthesis activating neuropeptide (PBAN). RNAi-based knockdown of MlorDES2 significantly reduced the titer of pheromone components by 58.6–85.9%, while knockdown of MlorDES9 specifically reduced the production of (Z)-9-tetradecenyl acetate. Functional verification in yeast revealed that MlorDES2 and MlorDES9 exhibited Δ11 and Δ9 desaturase activities, respectively. Taken together, these results collectively demonstrate that MlorDES2 and MlorDES9 are involved in sex pheromone biosynthesis of M. loreyi, suggesting that DESs could be used as potential targets for pest management.
研究领域:
昆虫学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
Insects
·
2025
·Vol. 16
·期 3
·307
·
IF 2.9
Fida Hussain Magsi,David R. Hall,Zongxiu Luo,Xiangfei Meng,Chunli Xiu,Zhaoqun Li,Lei Bian,Nanxia Fu,Jianlong Li,Zongmao Chen,Xiaoming Cai
Simple SummaryHelopeltis cinchonaeis an economically important pest of tea plantations in Asia and has recently been reported in various provinces of China, expanding its population range. The bugs feed on growing shoots, sucking the sap and leaving the shoots susceptible to infection by fungi. No known control or monitoring tools have been developed forH. cinchonaeexcept for the excessive application of pesticides. The components of the sex pheromone produced by femaleH. cinchonaehave been identified and synthesized in previous work and have been shown to attract male bugs to traps. This is the first time that the components of the sex pheromone have been identified for a species ofHelopeltisbug. Here, we present the results of field trapping tests to optimize the blend of pheromone components, compare different types of traps, and determine the best position of the trap in order to maximize the catch of male bugs. Traps baited with the optimized lure were used to monitor populations of the bug and showed two distinct generations during the year. These results contribute to an environmentally-friendly approach to monitoring and managingH. cinchonaein the field.Helopeltis cinchonaeis an emerging pest of tea and causes severe damage to tea plantations in China. The female ofH. cinchonaehas been reported to produce a sex pheromone consisting of two components, hexyl (3R)-3-acetoxybutyrate and (5R)-1-acetoxy-5-butyroxyhexane, and lures containing the synthetic compounds have been shown to attract maleH. cinchonaeto traps in the field. This is the first time that components of the sex pheromone have been identified for a species ofHelopeltisbug, but their field application has not been evaluated in detail. The present study shows that a blend of both compounds loaded into a polyethylene vial at 0.2 and 2 mg, respectively, caught significantly more maleH. cinchonaebugs than the individual compounds and all the other tested blends. Sticky wing traps baited with the binary blend of compounds at the optimized ratio and dosage caught more bugs than bucket funnel or delta traps, and traps hung at a height of 10 cm above the tea shoots caught more bugs than those at other heights. The optimized traps and lures were used for monitoring the pest and two distinct population peaks ofH. cinchonaewere observed, the first one during mid-May to early June, and the other one in mid-September. Overall, the results of this study contribute to an environmentally-friendly approach to monitoring and managingH. cinchonaein the field.
研究领域:
昆虫学
·
农业科学
·
害虫管理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）