Nature Communications
·
2022
·Vol. 13
·期 1
·1-10
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IF 17.694
Xiao Fuming,Gao Heqi,Lei Yunxiang,Dai Wenbo,Liu Miaochang,Zheng Xiaoyan,Cai Zhengxu,Huang Xiaobo,Wu Huayue,Ding Dan
Organic near-infrared room temperature phosphorescence materials have unparalleled advantages in bioimaging due to their excellent penetrability. However, limited by the energy gap law, the near-infrared phosphorescence materials (>650 nm) are very rare, moreover, the phosphorescence lifetimes of these materials are very short. In this work, we have obtained organic room temperature phosphorescence materials with long wavelengths (600/657–681/732 nm) and long lifetimes (102–324 ms) for the first time through the guest-host doped strategy. The guest molecule has sufficient conjugation to reduce the lowest triplet energy level and the host assists the guest in exciton transfer and inhibits the non-radiative transition of guest excitons. These materials exhibit good tissue penetration in bioimaging. Thanks to the characteristic of long lifetime and long wavelength emissive phosphorescence materials, the tumor imaging in living mice with a signal to background ratio value as high as 43 is successfully realized. This work provides a practical solution for the construction of organic phosphorescence materials with both long wavelengths and long lifetimes.
研究领域:
材料科学
·
生物医学工程
·
光学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Hongyu Chen,Peipei Tian,Jiayi Guo,Manman Sun,Wenping Zhu,Zhendong Li,Zengchen Liu
A highly sensitive and selective upconversion near-infrared (NIR) fluorescence and colorimetric dual readout hydrogen sulfide (H 2 S) nanoprobe was constructed based on the excellent NIR fluorescence emission performance of upconversion nanomaterials (UCNPs), the specific recognition effect of synergistically synthesized gold nanoflowers (trypsin-stabled AuNFs (Try-AuNFs)) and the effective NIR fluorescence quenching capability. In this assay, the sensing strategy included three processes. First of all, the synthesized UCNPs can emit 803 nm NIR fluorescence when they were excited by 980 nm excitation light. Secondly, as a result of the principle of fluorescence resonance energy transfer (FRET), Try-AuNFs can effectively quench the NIR fluorescence of UCNPs at 803 nm, which can effectively improve the signal-to-background ratio of nanoprobes, thereby improving the sensitivity of the probes. Thirdly, in the presence of H 2 S, the Try protective layer on the surface of Try-AuNFs was specifically penetrated, which will subsequently cleave Try-AuNFs via the strong S–Au bond. As such, the NIR fluorescence of UCNPs will be restored, achieving high selectivity and sensitivity detection of H 2 S. Under optimized conditions, the linear response range of H 2 S was 0.1–300 μM, and the detection limit was 53 nM. It is worth noting that the Try on the surface of Try-AuNFs via the synergistic effect can increase the steric hindrance of the probe, and this can effectively prevent the interaction between the probe with biothiols (cysteine (Cys), homocysteine (Hcy)) and other natural amino acids (non-thiol-containing) with resultant in the high selectivity regarding the detection of H 2 S in human serum, which is unlikely to be achieved by AuNFs synthesized by the gold seed method (Se-AuNFs). This work not only provided a new type of UCNPs fluorescence quencher and recognition unit, but also exemplified that the use of the physical properties (steric hindrance) of protein ligands on the surface of nanoflowers can improve the specificity of the probe. This will provide new ideas for the design of other nanoprobes.
研究领域:
纳米技术
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分析化学
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材料科学
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NATURE METHODS
·
2025
·
IF 32.1
Cao Ruijie,Li Yaning,Zhou Yao,Li Meiqi,Lin Fangrui,Wang Wenyi,Zhang Guoxun,Wang Gang,Jin Boya,Ren Wei,Sun Yu,Zhao Zhifeng,Zhang Wei,Sun Jing,Hou Yiwei,Xu Xinzhu,Hu Jiakui,Shi Wei,Fu Shuang,Liang Qianxi,Lu Yanye,Li Changhui,Zhao Yuxuan,Li Yiming,Kuang Dong,Wu Jiamin,Fei Peng,Qu Junle,Xi Peng
In fluorescence microscopy, a persistent challenge is the defocused background that obscures cellular details and introduces artifacts. Here, we introduce Dark sectioning, a method inspired by natural image dehazing for removing backgrounds that leverages dark channel prior and dual frequency separation to provide single-frame optical sectioning. Unlike denoising or deconvolution, Dark sectioning specifically targets and removes out-of-focus backgrounds, stably improving the signal-to-background ratio by nearly 10 dB and structural similarity index measure of images by approximately tenfold. Dark sectioning was validated using wide-field, confocal, two/three-dimensional structured illumination and one/two-photon microscopy with high-fidelity reconstruction. We further demonstrate its potential to improve the segmentation accuracy in deep tissues, resulting in better recognition of neurons in the mouse brain and accurate assessment of nuclei in prostate lesions or mouse brain sections. Dark sectioning is compatible with many other microscopy modalities, including light-sheet and light-field microscopy, as well as processing algorithms, including deconvolution and super-resolution optical fluctuation imaging.
研究领域:
显微镜技术
·
生物成像
·
计算成像
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细胞生物学
·
神经科学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）