CHEMISTRY & BIODIVERSITY
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2023
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IF 2.9
Yuan Liang,Zhuolin Li,Jie Zhang,Tiezhu Li,Chengyu Lv
Over the past decades, the synthetic glucocorticoids (GCs) have been widely used in clinical practice and animal husbandry. Given the health hazard of these toxic residues in food, it is necessary to explore the detailed interaction mechanisms of typical GCs and their main target glucocorticoid receptor (GR). Hence, this work compared the GR binding and agonist activities of typical GCs. Fluorescence polarization assay showed that these GCs were potent ligands of GR. Their GR binding affinities were in the order of methylprednisolone>betamethasone≈prednisolone>dexamethasone, with IC 50 values of 1.67, 2.94, 2.95, and 5.58 nM. Additionally, the limits of detection of dexamethasone, betamethasone, prednisolone, and methylprednisolone were 0.32, 0.14, 0.19, and 0.09 μg/kg in fluorescence polarization assay. Reporter gene assay showed that these GCs induced GR transactivation in a dose-dependent manner, confirming their GR agonist activities. Among which, dexamethasone at the concentration of 100 nM produced a maximal induction of more than 11-fold over the blank control. Molecular docking and molecular dynamics simulations suggested that hydrogen-bonding and hydrophobic interactions played an important role in stabilizing the GC-GR-LBD complexes. In summary, this work might help to understand the GR-mediated endocrine disrupting effects of typical GCs. Graphical
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PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2017
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IF 3.255
Yuan Huang,Zhiqun Zheng,Liying Huang,Hong Yao,Xiao shan Wu,Shaoguang Li,Dandan Lin
A rapid, simple, cost-effective dispersive liquid-phase microextraction based on solidified floating organic drop (SFOD-LPME) was developed in this study. Along with high-performance liquid chromatography, we used the developed approach to determine and enrich trace amounts of four glucocorticoids, namely, prednisone, betamethasone, dexamethasone, and cortisone acetate, in animal-derived food. We also investigated and optimized several important parameters that influenced the extraction efficiency of SFOD-LPME. These parameters include the extractant species, volumes of extraction and dispersant solvents, sodium chloride addition, sample pH, extraction time and temperature, and stirring rate. Under optimum experimental conditions, the calibration graph exhibited linearity over the range of 1.2–200.0 ng/ml for the four analytes, with a reasonable linearity(r 2 : 0.9990–0.9999). The enrichment factor was 142–276, and the detection limits was 0.39–0.46 ng/ml (0.078–0.23 μg/kg). This method was successfully applied to analyze actual food samples, and good spiked recoveries of over 81.5%–114.3% were obtained.
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PubMed
高影响因子
JOURNAL OF CHROMATOGRAPHY A
·
2025
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IF 4
Chunyi Yu,Xujing Kong,Wenting Li,Boyu Li,Baorong Chen,Mengyao Gao,Susu Cai,Yun Wei
Steroid hormones constitute a group of hormones with molecular weights ranging from 200 to 400 daltons, characterized by their highly similar chemical structures. Each hormone within this group holds significant value for the diagnosis of various diseases. Accurate clinical measurement of the levels of each hormone is crucial for the diagnosis in clinical settings. Due to the wide variety and different properties of steroid hormones in organisms, sample pretreatment is the rate-limiting step for analysis and detection. In this paper, a magnetic molecule-imprinting polymer (MMIPs) was prepared by surface imprinting on silicon coated magnetic spheres, and a detection method of MMIPs-combined with liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for 19 steroid hormones in plasma was developed and verified. Fourier transform infrared spectroscopy, field emission scanning electron microscopy and transmission electron microscopy were used to confirm the formation of MMIPs materials, and the conditions of material synthesis and pretreatment of steroid hormones from plasma were optimized. The detection method was evaluated by using linearity, LOD and LOQ, precision, accuracy and matrix effect. The linearity of 19 steroid hormones was good, and the linear correlation coefficient was greater than 0.995. The coefficients of variation were 2.1-9.8% and 1.1-9.7% for intra-day and inter-day, respectively, and the recoveries were 83.6%-118.2% at low concentration, and the recoveries of medium and high concentrations were 94.6%-113.8% and 89.5%-113.6%, respectively. The coefficient of variation of Relative matrix effect of 19 steroid hormones measured in different batches of plasma was 2.1-5.4% after isotope internal standard correction, which eliminated the influence of matrix effect.
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PubMed
高影响因子
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
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2025
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IF 3.3
Yirong Ma,Min Gong,Xinran Wei,Fan Yang,Yunzhe Li,Guanjun Nan,Zhimin Luo,Aiguo Zeng
A two-step electropolymerization strategy was constructed to establish an electrochemical sensor method for sensitive and selective detection of betamethasone. First, a poly-(2-aminoterephthalic acid) (P(ATA)) layer, which can significantly enhance electronic conductivity, was synthesized on the surface of the bare electrode using electropolymerization. Subsequently, taurine (Tau), which is rich in sulphonate groups, was electropolymerized and introduced into the P(ATA) layer to obtain P(Tau)/P(ATA)/GCE. Then, the electropolymerization parameters and test conditions were optimized, including the concentration of polymerization solution, the scanning rate, the polymerization cycles, buffer pH and accumulation time. Characterization of the obtained electrodes done using scanning electron microscopy and several electrochemical characterization methods proved the successful preparation of the P(Tau)/P(ATA)/GCE. Electrochemical tests of betamethasone at different concentrations (0.2-25μg·mL-1) under optimal measurement conditions exhibited excellent linearity, good sensitivity. Compared with commonly employed HPLC method, the proposed sensor method was much fast, simple, environmentally friendly, and cost-effective. Compared with other sensor methods, P(Tau)/P(ATA)/GCE sensor exhibited good sensitivity and excellent stability. Moreover, the P(Tau)/P(ATA)/GCE sensor was successfully applied to quantitatively detect betamethasone in cosmetics, which provides a new method for the monitoring of illegal addition of glucocorticoids in cosmetics.
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高影响因子
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