JOURNAL OF FOOD COMPOSITION AND ANALYSIS
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2024
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IF 4
Xinying Guo,Feng Chen,Shaocong Pan,Huamin Sun
A CoFe 2 O 4 /MEG solid-phase extraction combined with high performance liquid chromatography was used for determination of 8 biogenic amines (Bas) in canned Spanish mackerel products. A new comprehensive evaluation system, including dietary exposure, food safety index, chemical quality index and biogenic amine index, was used for investigating the pollution distribution, exposure and health assessment of BAs in four sea areas/provinces of China. The pollution levels in the Yellow Sea-Jiangsu, Bohai Sea-Shandong, Yellow and Bohai Sea-Liaoning regions were equivalent, with ∑8BAs equalling 34.7–36.5 mg/kg, while in the South China Sea-Guangdong region ∑8BAs was lowest at 18.9 mg/kg. The concentration range of ∑8BAs and ∑4BAs were ND (not detected)-113 mg/kg and ND-80.8 mg/kg, respectively. All results showed that the main components of BAs were putrescine, histamine, and spermine, with detection rates ranging from 23.1% to 42.3%. The dietary exposure level range of 8 BAs was 85.8–266 μg∙kg −1 ∙d −1 , IFSc was 0.00800–0.0248, CQI values range in the four regions was 1.21–3.93, with a mean CQI of 1.98. The total value of BAI ranges ND-0.840 mg/kg, with a mean BAI of 20.3 mg/kg. Statistical analysis showed that the overall health risk of BAs exhibits different trends in terms of gender, age, and region.
研究领域:
食品科学
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毒理学
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分析化学
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2025
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IF 6
Zhiyu Shao,Jie Zeng,Jun Dong,Fusui Ji,Hongxia Li,Ruiyue Yang,Wenxiang Chen,Wenyu Li,Siming Wang
Background Oxidative stress is a major mechanism underlying aging and health damage, and it is associated with decreased serum free thiol levels. Monitoring serum free thiol levels provides a valuable reference for assessing the body's health status. However, it is a key challenge in clinical analysis to simultaneously monitor serum total free thiols and specific low-molecular-mass free thiol compounds in a single run. Traditional methods often struggle to avoid interference from side reactions due to the high reactivity of free thiols in alkaline derivatization environments. Results In this study, we developed an integrated non-alkaline derivatization strategy using 2,2′-dithiodipyridine and high-performance liquid chromatography. 2,2′-Dithiodipyridine can react rapidly with free thiols under acidic to neutral environments, and all derivatives exhibit similar absorbance characteristics to the substrates. Taking advantage of these properties, this method combines reliable derivatization with efficient chromatographic separation, enabling the simultaneous analysis of total free thiols and five main specific low-molecular-mass thiol compounds within a single injection cycle. Eight chromatographic peaks including free thiol derivatives, the derivatization reagent, and internal standard were observed during a 14-minute analysis. The peak corresponding to 2-thiopyridone indicates the total concentration of free thiols while specific pyridyldithio derivative peaks represent the individual levels of low-molecular-mass free thiols. Using the developed method, a significant negative correlation was observed between free thiol levels and both age and health risk factors in a study involving 260 volunteers. Significance This study presents a robust and simple method for the simultaneous measurement of total free and low-molecular-mass free thiols, providing a facile technique for population analysis and for investigating the relationship between free thiols and human health.
研究领域:
分析化学
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生物医学
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临床检验
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DOI
PubMed
高影响因子
TALANTA
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2024
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IF 5.6
Xu Zhang,Yuanjiang Luo,Lei Xia,Yu Song,Wanjun Li,Lingwen Kong,Yawei Liu,Chengyin Shen,Chaoqun Huang,Yannan Chu
2,4,6-Trinitrotoluene (TNT) and its four metabolites, namely 2-ADNT, 4-ADNT, 2,4-DANT, and 2,6-DANT, are highly toxic substances. These metabolites also serve as biomarkers for assessing the health of individuals exposed to TNT. In this study, a homemade DDT-IMS apparatus was utilized to detect these metabolites. Under negative detection mode, the drift times of 2-ADNT and 4-ADNT showed subtle shifts within a drift tube temperature range of 100 °C–120 °C, aiding in their differentiation. In positive detection mode for 2,4-DANT and 2,6-DANT, significant variations were observed in both the number and drift time of their positive product ions across a drift tube temperature range of 80 °C–120 °C. Consequently, optimal analytical performance for these metabolites was achieved at approximately 100 °C. Evaluation of the instrumental response during the measurement of the four metabolites in both positive and negative modes revealed that negative detection mode offered greater advantages of detecting these compounds. The working ranges for measuring the four metabolites spanned two orders of magnitude, with detection limits for each metabolite nearly below 1 ng. Notably, clear identification of the signals for these metabolites was achieved even when samples were mixed in urine, highlighting the ability of the DDT-IMS in detecting TNT metabolites. The developed DDT-IMS detection method has significant potential for enhancing environmental risk assessment and biological hazard evaluation, particularly in relation to human exposure to TNT.
研究领域:
环境科学
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分析化学
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毒理学
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DOI
PubMed
高影响因子
ACS Sensors
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2024
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IF 8.2
Chaohui Tian,Saeed Ahmed Khan,Zhiyi Zhang,Xiaojing Cui,Hulin Zhang
Electronic skins (e-skins) are being extensively researched for their ability to recognize physiological data and deliver feedback via electrical signals. However, their wide range of applications is frequently restricted by the indispensableness of external power supplies and single sensory function. Here, we report a passive multimodal e-skin for real-time human health assessment based on a thermoelectric hydrogel. The hydrogel network consists of poly(vinyl alcohol)/low acyl gellan gum with [Fe(CN)6]4–/3– as the redox couple. The introduction of glycerol and Li+ furnishes the gel-based e-skin with antidrying and antifreezing properties, a thermopower of 2.04 mV K–1, fast self-healing in less than 10 min, and high conductivity of 2.56 S m–1. As a prospective application, the e-skin can actively perceive multimodal physiological signals without the need for decoupling, including body temperature, pulse rate, and sweat content, in real time by synergistically coupling sensing and transduction. This work offers a scientific basis and designs an approach to develop passive multimodal e-skins and promotes the application of wearable electronics in advanced intelligent medicine.
研究领域:
材料科学
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生物医学工程
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可穿戴电子
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健康监测
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DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
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2022
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IF 6.911
Jiani Li,Tingting Liu,Randy A. Dahlgren,Hanzhang Ye,Qi Wang,Yongli Ding,Ming Gao,Xuedong Wang,Huili Wang
We fabricated a novel N,S-co-doped carbon/Co 1-x S nanocomposite (NSC/Co 1-x S) using a facile sol-gel approach, which featured a multiporous structure, abundant S vacancies and Co-S nanoparticles filling the carbon-layer pores. When the Co 1-x S nanoparticles were anchored onto the surface of N,S-co-doped carbon, a synergistic catalysis action occurred. The NSC/Co 1-x S nanocomposites possessed both peroxidase-like and oxidase-mimetic dual-enzyme activities, in which the oxidase-mimetic activity dominated. By scavenger capture tests, the nanozyme was demonstrated to catalyze H 2 O 2 to produce h + , •OH and •O 2 − , among which the strongest and weakest signals were h + and •OH, respectively. The multi-valence states of Co atoms in the NSC/Co 1-x S structure facilitated electronic transfer that enhanced redox reactions , thereby improving the resultant color reaction. Based on the NSC/Co 1-x S's enzyme-mimetic catalytic reaction, a visual colorimetric assay and Android “Thing Identify” application (app), installed on a smartphone, offered detection limits of 1.93 and 2.51 mg/dl, respectively, in human serum samples. The selectivity/interference experiments, using fortified macromolecules and metal ions , demonstrated that this sensor had high selectivity and low interference potential for cholesterol analysis. Compared to standard assay kits and previously reported visual detection, the Android smartphone-based assays provided higher accuracy (recoveries up to 93.6–104.1%), feasibility for trace-level detection, and more convenient on-site application for cholesterol assay due to the superior enzymatic activity of NSC/Co 1-x S. These compelling performance metrics lead us to posit that the NSC/Co 1-x S-based nanozymic sensor offers a promising methodology for several practical applications, such as point-of-care diagnosis and workplace health evaluations.
研究领域:
材料科学
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生物化学
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分析化学
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生物医学工程
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即时检测
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）