In this work, a rapid, convenient and sensitive Au nanoparticle-based immunochromatographic sensor (AuNPs-ICS) for simultaneous detection of various tadalafil adulterants in health food was established for the first time, based on one high affinity and broad-spectrum antibody through skeleton-specific hapten design according to the common structure of tadalafil drugs. This immunochromatographic sensor was developed by the polyclonal antibody conjugated with AuNPs and the IC50 was 10.04 ng mL−1 for tadalafil and 9.55, 11.46, 17.01 ng mL−1 for amino tadalafil, acetamino tadalafil and nortadalafil, respectively. The limit of detection results could be obtained visually by naked eyes for and computationally by the hand-held immunochromatography reader. The sensor could specially detect Tadalafil and its analogs within 10 min in health food samples, and the close correlation with UPLC-MS/MS method showed the sensor was accurate and reliable, which could apply to on site detection Tadalafil and its analogs rapidly and accurately.
Yang Wang,Jinhua Wu,Cheng Xue,Ruoyu Wang,Tingting Wen,Junli Hong,Qin Hu,Fei Li,Xuemin Zhou
A novel dendrimer-based molecularly imprinted polymers (MIPs) coated dumbbell-shaped stir bar (DSB) was developed to enrich and analyze two kinds of phosphodiesterase type 5 (PDE-5) inhibitors in health foods (Hfs). The MIPs, with vardenafil as the template molecule and methacrylic acid (MAA) as the functional monomer, were synthesized on the surface of the dendritic-grafting DSB which was filled with magnetic core as substrate. The MIPs-DSB possessed mechanical stability, high adsorption capacity and good selectivity for vardenafil and its analogue sildenafil. A method for the determination of vardenafil and sildenafil in Hfs was established by stir bar sorptive extraction (SBSE) coupled with HPLC-UV. The contents of vardenafil and sildenafil were found to be 3.65 and 2.66 μg g−1, respectively, and the average recoveries in spiked Hfs were 86.5–91.8% with a relative standard deviation (RSD) below 5%. All results revealed that the MIPs-DSB coupled with HPLC-UV could be applied to the highly selective extraction and sensitive determination of trace analytes in a complex matrix.
危险品化学品经营许可证（带存储）