NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 36
·15782-15789
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IF 2.7
Hongling Tao,Yanyue Chen,Jiawen Liu,Jiali Liu,Yunhui Yang,Rong Hu
p53 gene is one of the most important anticancer genes and has been recognized as a typical biomarker for the early diagnosis of cancers. In this paper, a portable point-of-care testing (POCT) platform was developed for the colorimetric and photothermal dual-modal analysis of the p53 gene. Long concatemer G4 structures with high catalytic activity were formed by a target-driven hybridization chain reaction, and their catalytic properties can further be enhanced with the help of iodide ions. This dual-modal method realized the sensitive multicolor visual point-of-care detection of the p53 gene, where color variations from colourless to blue were easily distinguished by the naked eye. The temperature output was achieved by effective photothermal conversion and regulation guided by the accumulated concatemer G4 complexes in the TMB–H2O2 system, avoiding the need for complex instruments. The proposed dual-modal assay for p53 gene detection is cost-effective, simple, highly sensitive (3.34 pM), stable, and remarkably selective. It was also applied to determine the concentration of the p53 gene in spiked serum samples.
研究领域:
分子生物学
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癌症诊断
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生物化学
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Fang Li,Tianli Liu,Hong Wang,Yuming Dong,Guang-Li Wang
We propose herein an immobilization-free, split-mode cathodic photoelectrochemical (PEC) strategy coupled with a cascaded amplification for versatile biosensing. Taking DNA and microRNA (miRNA) as the model targets, the hybridization between the targets and the hairpin probe triggers the digestion of the probe DNA by T7 exonuclease (T7 Exo), thus to generate G-quadruplex (G4) forming sequences, and then the released targets (DNA or miRNA) initiate the subsequent cycling processes and generate a large amount of G4 forming sequences. Subsequently, the formed G4 sequences associate with hemin to form the G4/hemin DNAzyme, which catalytically produces 1,4-bezoquinone (BQ) for conjugating onto the surface of the chitosan (CS) deposited BiOI/ITO photocathode via the quinone-chitosan conjugation chemistry (QCCC). Under photo excitation, the covalently attached quinones can act as electron acceptors of bismuth oxyiodine (BiOI), promoting the photocurrent generation and thus allowing the elegant and “signal-on” mode for probing targets of interest. Highly sensitive and selective PEC bioassays are readily realized, with the detection limits down to 2.2 fM (for DNA) and 0.2 fM (for miRNA). Since no labeling and no electrode modification processes are needed, this split-mode PEC biosensing strategy is amenable to convenient, time/labor saving, and high-throughput detections. More significantly, it provides a novel concept to design immobilization-free and label-free cathodic PEC biosensing systems, and showcases promise in general and versatile bioanalysis research.
研究领域:
生物化学
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分析化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2024
·
IF 2.7
Na Qin,Sunlei Yang,Rui Li,Hongyun Zhan,Lijuan Wang,Fengyun Li,Jingbo Liu
The traditional method of acetamiprid residue detection is difficult to operate, time-consuming and laborious, and requires high professional knowledge of the detection personnel, which can no longer meet the rapid detection in the field. For this reason, a colorimetric aptasensor based on an entropy-catalyzed amplification system was developed for the ultrasensitive and selective determination of acetamiprid. In the absence of acetamiprid, the aptamer and cDNA build a double-stranded structure. The formed hemin/G-quadruplex mimicking DNAzyme can catalyze the substrate ABTS to generate the colored ion ABTS- with the help of H2O2, and the solution turns blue-green. On the contrary, the presence of acetamiprid triggers the release of cDNA, which in turn initiates the entropy-driven system, resulting in the inability to form DNAzyme and therefore no blue-green production of the solution. The quantity of acetamiprid determined the color period. Under the optimal experimental conditions, the method showed a linear correlation (R2=0.9837) for the detection of acetamiprid over the concentration range of 0.1 ~ 100 ng/mL, with a limit of detection of 0.06 ng/mL.The method was used for the determination of acetamiprid in spiked Coix lacryma and bitter almond, with the recoveries in the range of 90.3% ~ 110.3%. The proposed enzyme-free and label-free assay can be developed into a simple, sensitive and rapid detection platform.
研究领域:
分析化学
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环境科学
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生物技术
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2021
·
IF 7.514
Xinfa Cai,Meijuan Liang,Fei Ma,Zhaowei Zhang,Xiaoqian Tang,Jun Jiang,Can Guo,Sherif Ramzy Mohamed,Amira Abdel Goda,Dawood H. Dawood,Li Yu,Peiwu Li
Lateral flow immunoassay (LFIA) is the most effective real-time detection method for aflatoxin B 1 (AFB 1 ). Here, we constructed a nanozyme-strip based on MnO 2 nanosheets (MnO 2 NSs) as a catalytic label for detection of AFB 1 . By taking advantage of the MnO 2 -TMB catalytic amplified system, the new test achieves rapid detection with high sensitivity and ultrawide range. The limit of detection of the assay was 15 pg mL −1 , which was over 100-fold lower than the maximum limit set by the European Union (EU) of AFB 1 in foods. In addition, the strip test could offer 7 dynamic detection ranges, spanning 4 orders of magnitude, which could cater to the varieties of limits on AFB 1 residues in foods and feeds set by different countries. The estimated recoveries were in the range of 85.67%-106.38% with coefficients of variations (CVs) less than 9.68%. Overall, the developed approach is a rapid, reliable, sensitive and widely available tool for on-site detection of AFB 1 .
研究领域:
食品安全检测
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生物化学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Bing Zhang,Hongyun Meng,Xue Wang,Jing Li,Honghong Chang,Wenlong Wei
Herein we designed a novel photoelectrochemical sensing platform based on Fe 3+ doped ZnO-Ag photocatalyst (Fe 3+ /ZnO-Ag) for ultrasensitive Hg 2+ detection by using exonuclease III-assisted target recycling and DNAzyme-catalyzed amplification. Firstly, Fe 3+ /ZnO-Ag was synthesized to provide photocurrent under visible light irradiation. Secondly, target Hg 2+ was determined by hairpin DNA probes on the Fe 3+ /ZnO-Ag modified ITO based on T-Hg 2+ -T, and then Hg 2+ was released by the digestion of exonuclease III to double-stranded DNA. Finally, the photocurrent markedly decreased due to catalysis of hemin/G-quadruplex toward 4-chloro-1-naphthol. Under optimal conditions, the photocurrents were linearly related to Hg 2+ concentrations form 0. 5 nM to 100 nM with a detection limit of 0. 1 nM at the 3 S blank criterion. More importantly, the developed biosensor exhibited good selectivity, repeatability, and stability, meanwhile, this analysis platform held great potential for testing real water samples in future.
研究领域:
环境科学
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分析化学
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纳米技术
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DOI
高影响因子
JOURNAL OF NANOBIOTECHNOLOGY
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2022
·Vol. 20
·期 1
·1-12
·
IF 9.429
Yu Rongjun,Xue Jian,Wang Yang,Qiu Jingfu,Huang Xinyi,Chen Anyi,Xue Jianjiang
In this work, Ti 3 C 2 T x MXene was identified as efficient nanozyme with area-dependent electrocatalytic activity in oxidation of phenolic compounds, which originated from the strong adsorption effect between the phenolic hydroxyl group and the oxygen atom on the surface of Ti 3 C 2 T x MXene flake. On the basis of the novel electrocatalytic activity, Ti 3 C 2 T x MXene was combined with alkaline phosphatase to construct a novel cascading catalytic amplification strategy using 1-naphthyl phosphate (1-NPP) as substrate, thereby realizing efficient electrochemical signal amplification. Taking advantage of the novel cascading catalytic amplification strategy, an electrochemical biosensor was fabricated for BCR/ABL fusion gene detection, which achieved excellent sensitivity with linear range from 0.2 fM to 20 nM and limit of detection down to 0.05 fM. This biosensor provided a promising tool for ultrasensitive fusion gene detection in early diagnosis of chronic myelogenous leukemia and acute lymphocytic leukemia. Moreover, the manageable catalytic activity of MXene broke a path for developing nanozymes, which possessed enormous application potential in not only electrochemical analysis but also the extensive fields including organic synthesis, pollutant disposal and so on. Graphical Abstract
研究领域:
生物医学工程
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纳米材料科学
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癌症早期诊断
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DOI
PubMed
高影响因子
BIOELECTROCHEMISTRY
·
2024
·
IF 4.8
Guiyin Li,Huafu Feng,Xinhao Li,Shengnan Li,Jintao Liang,Zhide Zhou
Glypican-3 (GPC3) is an essential reference target for hepatocellular carcinoma detection, follow-up and prediction. Herein, a dual-signal electrochemical aptasensor based on reduced graphene oxide-cuprous oxide (RGO-Cu 2 O) nanozyme was developed for GPC3 detection. The RGO-Cu 2 O nanoenzyme displayed excellent electron transport effect, large specific surface area and outstanding peroxidase-like ability. The differential pulse voltammetry (DPV) signal of Cu 2 O oxidation fraction and the chronoamperometry ( i-t ) signal of H 2 O 2 decomposition catalyzed by RGO-Cu 2 O nanozyme were used as dual-signal detection. Under optimal conditions, the log-linear response ranges were 0.1 to 500.0 ng/mL with the limit of detection 0.064 ng/mL for DPV technique, and 0.1–50.0 ng/mL for i-t technique (detection limit of 0.0177 ng/mL). The electrochemical aptasensor has remarkably analytical performance with wide response range, low detection limit, excellent repeatability and specificity, good recovery in human serum samples. The two output signals of one sample achieve self-calibration of the results, effectively avoiding the occurrence of possible leakage and misdiagnosis of a single detection signal, suggesting that it will be a promising method in the early biomarker detection.
研究领域:
生物医学工程
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肿瘤学
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分析化学
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DOI
PubMed
高影响因子
ANALYST
·
2015
·Vol. 140
·期 22
·7710-7717
·
IF 4.107
Chunyan Qin,Wei Wen,Xiuhua Zhang,Haoshuang Gu,Shengfu Wang
A new class of strip biosensors has been established based on well-distributed thrombin aptamer-linked gold nanoparticle aggregates, which will undergo a cracking reaction when the target recognizes its homologous aptamer. Combining the aptamer-cleavage reaction with the enzyme catalytic amplification system, our proposed lateral flow strip biosensor (LFB) is capable of visually detecting 6.4 pM of thrombin without instrumentation within 12 minutes. Under the optimal conditions, the quantitative detection of thrombin by a portable strip reader exhibited a linear relationship between the peak area and the concentration of thrombin in the range of 6.4 pM–500 nM with a detection limit of 4.9 pM, which is three orders of magnitude lower than that of the aptamer-functionalized gold nanoparticle-based LFB (2.5 nM, Xu et al., Anal. Chem., 2009, 81, 669–675). As the aptamers have no special requirements and the gold nanoparticles can also be replaced by other metallic nanoparticles, this method for strip sensing is expected to be generally applicable in point of care testing, home testing, medical diagnostics, clinical diagnosis, and environmental monitoring.
研究领域:
生物医学工程
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分析化学
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即时检测
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医学诊断
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 12
·1-8
·
IF 5.833
Li Shuhuai,Pang Chaohai,Ma Xionghui,Zhang Yanling,Xu Zhi,Li Jianping,Zhang Meng,Wang Mingyue
The rapid detection of insecticides such as parathion-methyl (PM) requires methods with high sensitivities and selectivities. Herein, a dual catalytic amplification strategy was developed using Fe 3 O 4 nanozyme-supported carbon quantum dots and silver terephthalate metal–organic frameworks (Fe 3 O 4 /C-dots@Ag-MOFs) as current amplification elements. Based on this strategy, a novel electrochemical microfluidic paper-based chip was designed to detect PM. Fe 3 O 4 /C-dots@Ag-MOFs were synthesised by a hydrothermal method, and a molecularly imprinted polymer (MIP) was then synthesised on the surface of Fe 3 O 4 /C-dots@Ag-MOFs using PM as a template molecule. Finally, the reaction zone of a chip was modified with MIP/Fe 3 O 4 /C-dots@Ag-MOFs. PM from a sample introduced into the reaction zone was captured by the MIP, which generated a reduction current response at − 0.53 V in a three-electrode system embedded in the chip. Simultaneous catalysis by Fe 3 O 4 /C-dots and Ag-MOFs significantly enhanced the signal. The chip had a detection limit of 1.16 × 10 −11 mol L −1 and was successfully applied to the determination of PM in agricultural products and environmental samples with recovery rates ranging from 82.7 to 109%, with a relative standard deviation (RSD) of less than 5.0%. This approach of combining a dual catalytic amplification strategy with an MIP significantly increased the sensitivity as well as selectivity of chips and can potentially be used to detect a wide variety of target analytes using microfluidic paper-based chips. Graphical abstract
研究领域:
分析化学
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环境科学
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材料科学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Yanlian Niu,Zezhao Meng,Guofeng Qiu,Han Zhao,Shulan Liu,Nan Sun,Wei Qin
A high sensitive sandwich-type electrochemical biosensor utilizing capture probe (CP), target probe (TP), and signal probe (SP) was first developed for specific detection of microRNA-26a (miR-26a), a biomarker with diagnostic significance for atherosclerosis. In general, gold nanoparticles (Au NPs) were electrodeposited on to a glassy carbon electrode to enhance conductivity, followed by immobilization of 5 ′- thiolated CP through a gold-sulfur bond. Subsequently, the modified electrode was hybridized with the mixture of miR-26a and 3′-biotin-tagged SP in sequence. Furthermore, horseradish peroxidase-streptavidin (HRP) with potent reducing capability was finally grafted on the SP via the strong affinity between biotin and streptavidin. Additionally, the fabrication process of the miR-26a biosensor was thoroughly investigated. Based on the dual amplification mechanism facilitated by Au NPs and HRP-catalytic activity, the proposed biosensor demonstrated outstanding electrochemical properties, including good reproducibility and stability, as well as high sensitivity for miR-26a detection down to 0.88 fM with a linear range from 1 fM to 1 nM. The biosensor also displayed exact specificity, which could discriminate miR-26a from a range of base mismatched microRNAs. Moreover, it was successfully applied to the determination of miR-26a in serum samples. In conclusion, given the excellent analytical performance, this biosensor shows promise for potential use in clinical diagnostics for atherosclerosis.
研究领域:
生物化学
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纳米技术
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生物医学工程
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分析化学
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临床诊断
✓ 已验证
产品关联
DOI
高影响因子
ACS Nano
·
2025
·
IF 16
Min Wu,Songlong Jiao,Lei Liu
Nitrogen-vacancy (NV) centers in diamond demonstrate advantages in biosensing due to their exceptional photostability and long spin coherence time. However, clinical applications of NV centers are significantly limited because their spin states lack responsiveness to nonmagnetic biomolecules. This work presents a nanocatalytic-amplified quantum sensing platform targeting tyrosinase (TYR), a key biomarker for melanoma. The platform achieves ultrasensitive detection of nonmagnetic biomarkers through nanocatalytic signal transduction. Specifically, we develop plasmonic nanodiamond (FND@Au–Ag) functionalized hydrogel microneedles. The captured TYR triggers a cascade reaction that induces the nanocatalytic generation of hydroxyl radicals (•OH), thereby establishing an AND logic gate based on the dynamic response mechanism of spin relaxation time (T1). By integrating three-modal signals (fluorescence, colorimetry, and T1), the platform achieves a wide dynamic detection range of 0.01–200 U/mL and an ultralow detection limit (0.003 U/mL) for TYR. Ultimately, a genetic algorithm-optimized backpropagation neural network is developed to enhance the reliability of concentration prediction in complex biological matrices. This work proposes an approach to minimally invasive melanoma screening, overcoming the inherent limitations of traditional quantum sensing systems in detecting nonmagnetic biomarkers.
研究领域:
生物医学工程
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量子物理
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纳米技术
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生物传感
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肿瘤学
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分析化学
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2021
·
IF 9.229
Xionghui Ma,Chaohai Pang,Shuhuai Li,Jianping Li,Mingyue Wang,Yuhao Xiong,Linjing Su,Jinhui Luo,Zhi Xu,Liyun Lin
Metal–organic frameworks (MOFs) prepared via typical procedures tend to exhibit issues like poor water stability and poor conductivity, which hinder their application in electrochemical sensing. Herein, we report a strategy for the preparation of mixed-valence ultrafine one-dimensional Ce-MOF nanowires based on a micelle-assisted biomimetic route and subsequent investigation into their growth mechanism. The prepared mixed-valence Ce-MOF nanowires exhibited a typical size of ∼50 nm and were found to present good water stability and high conductivity. On this basis, we examined the introduction of these nanowires into the luminol hydrogen peroxide luminescence system and proposed a novel dual-route self-circulating electrochemiluminescence (ECL) catalytic amplification mechanism. Finally, in combination with molecular imprinting, a MOF-based ECL sensor was developed for the detection of trace amounts of imidacloprid in plant-derived foods. This sensor exhibited a linearity of 2–120 nM and a detection limit of 0.34 nM. Thus, we proposed not only a novel route to MOF downsizing but also a facile and robust methodology for the design of a MOF-based molecular imprinting ECL sensor.
研究领域:
材料科学
·
分析化学
·
纳米技术
·
环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）