Polymers
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2018
·Vol. 10
·期 3
·260
·
IF 2.935
Lanlan Wang,Artur Cavaco-Paulo,Bo Xu,Madalena Martins
Polymeric hydrogel based on acrylic acid (AA) andN,N-dimethylacrylamide (DMAA) was prepared by photopolymerization reaction, using nano-alumina as the inorganic crosslinker. Hydrogel-coated wool yarns determine their dimensional changes under humidity conditions. Surface morphology of the hydrogel-coated wool yarns was carried out using SEM microscopy. The hydrogel was further characterized by Fourier transformer infrared spectrum (FTIR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), thermogravimetry (TG) and differential thermogravimetry (DTG). This contribution showed that UV-initiated polymerization coating wool yarns can change the functional properties of wool fibers.Keywords:hydrogel;wool yarns;photocrosslinking;UV polymerization
研究领域:
材料科学
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纺织工程
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产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2016
·Vol. 7
·期 1
·1-10
·
IF 11.329
Yang Yi,Song Haiping,He Dan,Zhang Shuai,Dai Shizhong,Lin Shixian,Meng Rong,Wang Chu,Chen Peng R.
Coupling photocrosslinking reagents with mass spectrometry has become a powerful tool for studying protein–protein interactions in living systems, but it still suffers from high rates of false-positive identifications as well as the lack of information on interaction interface due to the challenges in deciphering crosslinking peptides. Here we develop a genetically encoded photo-affinity unnatural amino acid that introduces a mass spectrometry-identifiable label (MS-label) to the captured prey proteins after photocrosslinking and prey–bait separation. This strategy, termed IMAPP ( I n-situ cleavage and M S-label transfer A fter P rotein P hotocrosslinking), enables direct identification of photo-captured substrate peptides that are difficult to uncover by conventional genetically encoded photocrosslinkers. Taking advantage of the MS-label, the IMAPP strategy significantly enhances the confidence for identifying protein–protein interactions and enables simultaneous mapping of the binding interface under living conditions.
研究领域:
生物化学
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分子生物学
·
蛋白质组学
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产品关联
DOI
PubMed
高影响因子
Applied Materials Today
·
2024
·
IF 7.2
Zhenxi Liu,Dong Zhang,Huili Kang,Xinyi Gu,Fangping Chen,Changsheng Liu
The restoration of articular cartilage poses a significant clinical challenge due to its avascular nature and the limited presence of chondrocytes. Recently, there has been a growing emphasis on developing materials that emulate the properties of cartilage to facilitate high-quality repair. Here, an injectable dECM-loaded hydrogel (HS-dECM) with potent compressive strength and self-lubricating ability was prepared by photo-crosslinking for cartilage regeneration. The HS-dECM hydrogel was formed by integrating cartilage decellularized extracellular matrix (dECM) with hyaluronic acid methacrylate (HAMA) and sulfobetaine methacrylate (SBMA). Once injected into irregular defects, a robust hydrogel was rapidly formed in situ by UV irradiation with negligible heat release. The HS-dECM hydrogel exhibited robust compressive strength (898 ± 86 kPa), excellent fatigue resistance and a low friction coefficient, and had the feasibility of minimally invasive implantation. Besides, HS-dECM hydrogel promoted chondrogenic differentiation of BMSCs in vitro and repaired articular cartilage defects in rats. Therefore, the high-performance injectable HS-dECM hydrogel represents a novel minimally invasive strategy that significantly promote the cartilage injuries repair and regeneration.
研究领域:
生物材料学
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组织工程
·
再生医学
✓ 已验证
产品关联
DOI
高影响因子
CARBOHYDRATE POLYMERS
·
2012
·
IF 3.628
Tao Wang,Xueyan Mu,Haibo Li,Weilong Wu,Jun Nie,Dongzhi Yang
We developed a copolymeric bioadhesive system with the potential to be used as a tissue adhesive based on biopolymer dextran. Copolymeric hydrogels comprising a urethane dextran (Dex-U) and 2-hydroxyethyl methacrylate (HEMA) were prepared and crosslinked under the ultraviolet (UV) irradiation. In this study, the photopolymerization process was monitored by real time infrared spectroscopy (RTIR). The adhesion strength was evaluated by lap-shear-test. The surface tension, viscosity of the solutions and the cytotoxicity assays were investigated. Compared to Dex-U system, the addition of HEMA remarkably improved the properties of Dex-H system especially the adhesion strength and the nontoxicity. And materials variation could be tailored to match the need of tissues. The copolymeric tissue adhesives demonstrated promising adhesion strength and nontoxicity. The maximum adhesion strength reached to 4.33 ± 0.47 Mpa which was 86 times higher than that of Tisseel. The obtained products have the potential to serve as tissue adhesive in the future.
研究领域:
生物材料学
·
组织工程学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Bio-Design and Manufacturing
·
2024
·
IF 8.1
Xu Tao,Yang Changwei,Lu Yang,Wang Heng,Chen Cheng,Zhou Yuchen,Chen Xiaoqing
The recovery and reconstruction of central nervous system function after spinal cord injury (SCI) is a worldwide problem. The difficulty lies in the feasibility issue of new axons passing through the injured area and the negative effect of scarring after injury. As a biological material, the human amniotic membrane (HAM) has the advantages of protecting nerve growth, inhibiting scar formation, and promoting neovascularization, but its weak physical properties are difficult to apply in treating SCI. In this study, HAMs were first decellularized and then chemically grafted with methacrylic anhydride. Next, the composite was photocrosslinked with gelatin methacrylate to prepare a cross-network biological complex. The final complexes prepared by appeal were used for in vitro and in vivo studies of SCI in rats, separately. In the in vitro experiment, the composite scaffold inherited abundant biological factors from the amniotic membrane and had the physical properties of a hydrogel, thus providing a favorable environment for the growth and development of neurons and blood vessels. In the in vivo experiment, the composite reduced scarring and promoted the growth of new nerves. Overall, the composite scaffolds can stably simulate the extracellular microenvironment in SCI defects, regulate pathological changes, and promote the generation of new neurons. Therefore, decellularized HAM hydrogels are promising biocomposite materials for central nerve repair after SCI. Graphic abstract
研究领域:
生物材料学
·
神经科学
·
再生医学
✓ 已验证
产品关联
DOI
高影响因子
Acta Biomaterialia
·
2021
·
IF 8.947
Qiang Zhang,Caiwang Chang,Chunyu Qian,Wanshu Xiao,Huajun Zhu,Jun Guo,Zhibing Meng,Wenguo Cui,Zili Ge
The human amniotic membrane (HAM) collagen matrix derived from human placenta can be decellularized (dHAM) to form a natural biocompatible material. dHAM has different bioactive substances and has been used widely in human tissue engineering research. However, dHAM has some disadvantages, e.g., poor mechanical properties , easy degradation and inconvenient operation and use, so it is not conducive to large-area or full-thickness skin defect healing. To overcome these limitations, for the first time, dHAM was grafted with methacrylic anhydride (MA) to form photocrosslinked dHAM methacrylate (dHAMMA); dHAMMA was then blended with methacrylated gelatin (GelMA), followed by the addition of a photosensitizer for photocrosslinking to obtain the fast-forming GelMA-dHAMMA composite hydrogel. Further, GelMA-dHAMMA was found to have the porous structure of a bicomponent polymer network and good physical and chemical properties. In vitro experiments, GelMA-dHAMMA was found to promote fibroblast proliferation and α-smooth muscle actin (α-SMA) expression. In vivo investigations also demonstrated that GelMA-dHAMMA promotes wound collagen deposition and angiogenesis , and accelerates tissue healing. GelMA-dHAMMA inherits the good mechanical properties of GelMA and maintains the biological activity of the amniotic membrane, promoting the reconstruction and regeneration of skin wounds. Thus, GelMA-dHAMMA can serve as a promising biomaterial in skin tissue engineering. Statement of significance Since the early 20th century, the human amniotic membrane (HAM) has been successfully used for trauma treatment and reconstruction purposes. dHAM has different bioactive substances and has been used widely in human tissue-engineering research. In this work, the dHAM and gelatin were grafted and modified by using methacrylic anhydride (MA) to form photocrosslinked dHAMMA and methacrylated gelatin (GelMA). Then, the dHAMMA and GelMA were blended with a photosensitizer to form the GelMA-dHAMMA composite hydrogel derived from gelatin-dHAM. GelMA-dHAMMA exhibits a bicomponent-network (BCN) interpenetrating structure. dHAM dydrogel has advantages, e.g., good mechanical properties, slow degradation and convenient operation, so it is conducive to large-area or full-thickness skin defect healing.
研究领域:
生物医学工程
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组织工程
·
再生医学
✓ 已验证
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2018
·
IF 4.439
Zhang Ganwei,Bai Renbi,Shen Shusu,Zhou Xiaoji,Guo Yongfu
Bifunctional copolymers bearing hydrophilic units and photo-crosslinkable units were prepared and employed to coat PVDF membranes and thus improve their antifouling properties and coating stability. A series of methoxy poly(ethylene glycol)- block -poly(2-hydroxyethyl methacrylate) (mPEG- b -PHEMA) diblock copolymers were prepared via the atom transfer radical polymerization (ATRP) of 2-hydroxyethyl methacrylate (HEMA) using different mPEG macroinitiators, and then reacted with cinnamoyl chloride to obtain methoxy poly(ethylene glycol)- block -poly(2-cinnamoyloxyethyl methacrylate) (mPEG- b -PCEMA). Coating PVDF microfilter membranes with mPEG- b -PCEMA and subsequently photo-crosslinking the PCEMA block yielded crosslinked copolymer coatings. The successful preparation of these coatings was confirmed by SEM, TGA, ATR-FTIR and XPS characterization. The hydrophilicity of the membrane increased along with the amount of diblock copolymer coating on the coated PVDF membrane. Based on the results of BSA adsorption and filtration experiments, the antifouling properties of the coated PVDF membrane remarkably improved. Furthermore, the mPEG block chain length and its content in the diblock copolymer had a significant effect on the surface properties of the coated PVDF membrane. More importantly, the copolymer coatings were very stable thanks to the crosslinking of the PCEMA chains of the diblock copolymer.
研究领域:
材料科学
·
环境工程
✓ 已验证
DOI
高影响因子
CELLULOSE
·
2015
·Vol. 22
·期 6
·3877-3894
·
IF 3.573
Bai Huiyu,Li Yufei,Wang Wei,Chen Guangliang,Rojas Orlando J.,Dong Weifu,Liu Xiaoya
A novel method for the preparation of composites based on poly(vinyl alcohol) (PVA) and micro- and nano-fibrillated cellulose (M/NFC) is reported. The addition of crosslinking HEMA monomers and a photo-initiator enabled the formation of an interpenetrated polymer network that displayed enhanced interfacial adhesion and dispersion of (M/NFC) in the matrix. Photo-crosslinked PVA/(M/NFC)/polyHEMA composite films produced by casting followed by UV polymerization of HEMA were characterized by using several approaches. These included Fourier transform infrared and UV–Vis spectroscopies, differential scanning calorimetry, X-ray diffractometry, thermogravimetric analysis and dynamic mechanical analysis. The thermo-mechanical and optical properties as well as water absorption and vapor barrier abilities of the developed photo-crosslinked PVA/(M/NFC)/polyHEMA films were measured and the benefit of photo-crosslinking determined. The major degradation peak of photo-crosslinked PVA/(M/NFC)/polyHEMA composites increased substantially compared to that of the PVA/(M/NFC) system (from ~300 to ~350 °C). The water vapor permeability of PVA/(M/NFC)/polyHEMA composite films was reduced with HEMA loading (5.44 × 10 −11 , 5.10 × 10 −11 , 4.12 × 10 −11 , 4.31 × 10 −11 g/m h Pa for 0, 5, 10 and 15 % HEMA, respectively). Overall, a new and facile method for the synthesis of PVA/(M/NFC)-based composite networks interpenetrated with cross-linked poly(HEMA) is demonstrated, offering excellent prospects for packaging applications.
研究领域:
材料科学
·
包装工程
✓ 已验证
DOI
高影响因子
LANGMUIR
·
2017
·
IF 3.833
Zongpeng Yang,Xiaoli Chen,Zhou Xu,Meina Xiao,Liangzhi Hong,To Ngai
In this work, we have investigated the effect of laminar flow shearing on the formation of block copolymer agglomerates in viscous medium. Under a laminar flow shearing, the block copolymer solution droplets were spontaneously emulsified and were then elongated into protofibers, which in turn transformed into particles with various morphologies. Besides micro-/nanorods, which were previously reported for homopolymers, sphere and sheetlike structures were unexpectedly fabricated from block copolymers depending on the solvent quality, solvent exchange rates, and the entanglement of the polymer chains. In particular, the sheet structure, fabricated from poly(ethylene glycol)-b-polystyrene (PEG-b-PS), can be fixed by UV irradiation when photo-crosslinkable azide groups were introduced onto the polystyrene block. Surprisingly, we found that the fixed sheetlike structures show demulsification capability in tens of seconds, which may have great potential application in the separation of oil from emulsions.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LIQUID CRYSTALS
·
2019
·
IF 3.078
Peng Wei,Yanping Wang,Yumin Xia,Yong He,Yimin Wang
Firstly, PEGn-TA monomers with carboxyl functional groups were synthesised by terephthalic acid (TA) and poly(ethylene glycol) (PEG) with molecular weight of 200, 400 and 600. Then, photocrosslinkable thermotropic liquid crystal copolyesters derived from PEGn-TA, 4,4ʹ-biphenol, 4-hydroxy-cinnamic acid and 4-hydroxy-benzoic acid were successfully obtained by Vilsmeier adduct solution polymerisation method. Influence of PEG molecular weight on thermal behaviour, liquid crystal mesophase, hydrophilicity, structure and photoactivity were investigated by various measurements. Experimental results indicated that all copolyesters showed nematic mesophase textures, relative low melting temperatures, and two glass transitions as well as better hydrophilicity were observed for those contained higher PEG molecular weight. Photocrosslinking rate was increased first and then decreased due to effect of flexible PEG moieties. The resulted copolyesters with liquid crystal behaviour, excellent hydrophilicity and photoactivity could be potentially used as biomaterial.
研究领域:
材料科学
·
高分子化学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2024
·
IF 10
Qian Han,Jiahui He,Lang Bai,Ying Huang,Baojun Chen,Zhenlong Li,Meiguang Xu,Qiaonan Liu,Shuai Wang,Nuanyang Wen,Jing Zhang,Baolin Guo,Zhanhai Yin
Partial-thickness cartilage defect (PTCD) is a common and formidable clinical challenge without effective therapeutic approaches. The inherent anti-adhesive characteristics of the extracellular matrix within cartilage pose a significant impediment to the integration of cells or biomaterials with the native cartilage during cartilage repair. Here, an injectable photocrosslinked bioadhesive hydrogel, consisting of gelatin methacryloyl (GM), acryloyl-6-aminocaproic acid- g -N-hydroxysuccinimide (AN), and poly(lactic- co -glycolic acid) microspheres loaded with kartogenin (KGN) (abbreviated as GM/AN/KGN hydrogel), is designed to enhance interfacial integration and repair of PTCD. After injected in situ at the irregular defect, a stable and robust hydrogel network is rapidly formed by ultraviolet irradiation, and it can be quickly and tightly adhered to native cartilage through amide bonds. The hydrogel exhibits good adhesion strength up to 27.25 ± 1.22 kPa by lap shear strength experiments. The GM/AN/KGN hydrogel demonstrates good adhesion, low swelling, resistance to fatigue, biocompatibility, and chondrogenesis properties in vitro. A rat model with PTCD exhibits restoration of a smoother surface, stable seamless integration, and abundant aggrecan and type II collagen production. The injectable stable adhesive hydrogel with long-term chondrogenic differentiation capacity shows great potential to facilitate repair of PTCD.
研究领域:
生物医学工程
·
组织工程
·
再生医学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2020
·
IF 2.69
Meijie Wang,Kaiwei He,Jilu Li,Tong Shen,Yang Li,Yiting Xu,Conghui Yuan,Lizong Dai
In this article, we introduce a pH-responsive charge-reversible and photo-crosslinkable polymer nanoparticle. It is prepared via typical self-assembly from a block copolymer poly((7-(4-vinyl-benzyloxyl)-4-methylcoumarin)-co-acrylicacid)-b-poly((2-dimethylamino) ethyl methacrylate)-co-styrene) (P(VBMC-co-AA)-b-P(DMAEMA-co-St)), whose two blocks have different ionizable moieties. In an aqueous solution of pH ≤ 4, the cationic polymer nanoparticles are formed due to the fully protonated PDMAEMA. At a pH ranging from 5.0 to 7.8, partially ionized PAA and protonated PDMAEMA lead to the formation of polymer nanoparticles with a mixed shell. In a pH range of 8–10, a large amount of precipitation is produced within the isoelectric point (IEP) region because of the weak hydrophilic two blocks. In an aqueous solution of pH ≥ 10, polymer nanoparticles are reformed with PAA shell and P(DMAEMA-co-St) core. The coumarin groups of polymer can undergo photo-crosslinking and photo-cleavage reactions under UV light irradiation at λ = 365 nm and λ = 254 nm, respectively. The reversible nature of the photo-reaction can regulate the reversal of polymer nanoparticles. Furthermore, the aggregation-induced fluorescence emission (AIFE) property of polymer nanoparticles at different pH is tested by fluorescence emission spectra. The results indicate that the aggregation state of coumarin blocks in solution also changes with the pH value. The DOX release experiment in vitro shows that the release behavior of DOX-loaded nanoparticles can be adjusted by pH and light to achieve significant control. The inhibitory effect on the growth of tumor cells is displayed by cellular uptake and cytotoxicity test in vitro. The self-assembly system of polymer nanoparticles can be cooperatively controlled by multiple stimulations and displays potential applications in controlled drug delivery.
研究领域:
材料科学
·
药学
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）