Agronomy-Basel
·
2024
·Vol. 14
·期 2
·399
·
IF 3.3
Nan Zhang,Yawei Yang,Xin Wang,Taozhong Shi,Pei Lv,Qing X. Li,Rimao Hua
Profenofos is a detectable insecticide in the environment with strong toxicity to non-targeted organisms. Photodegradation is a main transformation of profenofos in the environment. Myricetin is a flavonoid that strongly scavenges free radicals. The effect of myricetin on the photodegradation of profenofos was studied. The half-lives (T1/2) of profenofos were 1.7–7.0 and 90 h under artificial light and sunlight. The photolysis rate of profenofos decreased by 1.87–4.72 and 7.62 times with the addition of 20 ratios of myricetin. Free radicals reacting with profenofos were •OH and1O2, and the key free radical was •OH. Myricetin strongly scavenged •OH and1O2which rapidly reacted with profenofos.O-(2-Chlorophenyl)-O-ethyl-S-propyl phosphorothioate (M3) andO-(2-chlorophenyl)-O-ethyl phosphorothioate (M4) were major and new photoproducts of profenofos. According to the Ecological Structure Activity Relationships, photodegradation of profenofos was a detoxification process, but myricetin inhibited the photodegradation of profenofos and its photoproducts. These results highlight the implication of myricetin on the fate and potential risk of profenofos in the environment.
研究领域:
环境科学
·
农药化学
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Hongrui Zhang,Ya-nan Zhang,Like Li,Xuegang Li,Yong Zhao
A method for the determination of cholesterol using rhodamine derivatives as chromogenic substrates was proposed in this study. The oxidation of rhodamine B hydrazide (RBH) by hydrogen peroxide (H2O2) was catalyzed by a peroxide-mimicking enzyme to produce the fluorescent product. The reaction was further combined with the oxidase system to achieve indirect determination of cholesterol concentration, which can be catalyzed by cholesterol oxidase to produce H2O2 as an intermediate product. Optical fiber optofluidic lasers (FOFLs), as a sensitive biochemical detection platform, have the ability to distinguish small differences in the amount of fluorescence products generated by the reaction and were used to achieve sensitive cholesterol detection. Under optimized experimental conditions, the dynamic range of three orders of magnitude was obtained in the determination of H2O2 with a lower limit of detection (LOD) of 1.63 μM, and the assay time was as short as 3.5 min. On this basis, the designed cholesterol sensor obtained a linear range of 2.55–652.67 μM with a lower LOD of 2.55 μM and proved to have good selectivity. The possibility of cholesterol determination in human serum was initially tested. The sensor was also characterized by easy signal collection, wash-free procedure, controlled sample consumption, fast assay, low cost, and simple operation.
研究领域:
生物化学
·
医学检测
·
光学传感
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产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2016
·Vol. 21
·期 10
·1345
·
IF 2.465
Meng Shi,Ying Nie,Xin-Qiang Zheng,Jian-Liang Lu,Yue-Rong Liang,Jian-Hui Ye
Ultraviolet B (UVB) photosensitivities of eight catechins were screened. In both water and ethanol, epicatechin (EC, 575 μM) and catechin (C, 575 μM) exhibited low photostabilities under 6 h UVB radiation with the generation of yellow photoproducts, while other catechins (epigallocatechin gallate, epigallocatechin, epicatechin gallate, gallocatechingallate, gallocatechin, catechin gallate) were relatively UVB-insensitive. Photoisomerization and photolysis were two important UVB-induced reactions to EC whereas photolysis was the dominant reaction for C. The influencing factors of time (2–10 h), solvent (water, ethanol) and substrate concentration (71.875–1150 μM) on UVB-induced chemical conversions of EC and C were investigated, and eight photoproducts were identified through ultra performance liquid chromatography-diode array detection-tandem mass spectrometry (UPLC-DAD-MS/MS) and1H nuclear magnetic resonance (1H-NMR analysis). Photolysis reaction involved two pathways, including radical reaction and photo-induced electron transfer reaction. The 2,2-diphenylpicrylhydrazyl (DPPH) scavenging abilities of eight catechins did not change upon 6 h UVB irradiation. EC and C are photosensitive catechins among eight catechins causing deep color.Keywords:photostability;catechins;photoisomerization;photolysis;photoproducts
研究领域:
食品科学
·
植物化学
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产品关联
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2023
·
IF 8.8
Weiming Xiang,Fahao Xu,Dong Wan,Xing Wang,Fan Luo,Yong Chen
Chloroquine phosphate (CQ) is an antiviral drug for Coronavirus Disease 2019 and an old drug for treatment of malaria, which has been detected in natural waters. Despite its prevalence, the environmental fate of CQ remains unclear. In this study, the direct photodegradation of CQ under simulated sunlight was investigated. The effect of various parameters such as pH, initial concentration and environmental matrix were examined. The photodegradation quantum yield of CQ (4.5 × 10 −5 −0.025) increased with the increasing pH value in the range of 6.0–10.0. The electron spin resonance (ESR) spectrometry and quenching experiments verified that the direct photodegradation of CQ was primarily associated with excited triplet states of CQ ( 3 CQ*). The common ions had negligible effect and humic substances exhibited a negative effect on CQ photodegradation. The photoproducts were identified using high-resolution mass spectrometry and the photodegradation pathway of CQ was proposed. The direct photodegradation of CQ involved the cleavage of the C–Cl bond and substitution of the hydroxyl group, followed by further oxidation to yield carboxylic products. The photodegradation processes were further confirmed by the density functional theory (DFT) computation for the energy barrier of CQ dichlorination. The findings contribute to the assessment of the ecological risk associated with the overuse of Coronavirus drugs during global public health emergencies.
研究领域:
环境科学
·
药理学
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Ning Zhang,Wenchao Deng,Yao Li,Yangde Ma,Yan Liu,Xiangjun Li,Hailin Wang
Cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6–4) pyrimidone photoproducts (6–4PPs) are genotoxic DNA lesions and mainly generated on thymine–thymine (T–T) dinucleotides upon UV irradiation. Regarding the sensitivity, specificity, and accuracy of analytical methods, it is of first choice to develop a reliable assay for simultaneous detection of these DNA lesions using liquid chromatography-tandem mass spectrometry (LC-MS/MS). However, the dilemma is the low detection sensitivity of the phosphate-containing dimeric photoproducts even using most favorable negative-ion mode for LC-MS/MS analysis. Unexpectedly, we observed that the detection sensitivity of T–T CPD and 6–4PP could be significantly improved using formic acid/acetic acid (∼ppm) as an additive of the mobile phase for reversed-phase LC-MS/MS analysis. This is the first demonstration of the enhancement of LC-MS/MS signals by formic acid/acetic acid in negative-ion mode. Of note, these acidic agents are often used for positive-ion mode in LC-MS assays. Benefited from the developed method, we could quantify both T–T CPD and 6–4PP in mouse embryonic stem cells upon UVC irradiation at low dosage. This sensitive method is applicable to the screening and identification of genes involved in formation, signaling, and repair of UV lesion.
研究领域:
生物化学
·
分子生物学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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