Molecular Plant
·
2024
·
IF 17.1
Xiaoming Li,Zhonghui Chen,Haiyang Li,Lin Yue,Cuirong Tan,Hongjie Liu,Yilong Hu,Yuhua Yang,Xiani Yao,Lingping Kong,Xiang Huang,Bin Yu,Chunyu Zhang,Yuefeng Guan,Baohui Liu,Fanjiang Kong,Xingliang Hou
Soybean is a photoperiod-sensitive short-day crop whose reproductive period and yield are markedly affected by day-length changes. Seed weight is one of the key traits determining the soybean yield; however, the prominent genes that control the final seed weight of soybean and the mechanisms underlying the photoperiod’s effect on this trait remain poorly understood. In this study, we identify SW19 as a major locus controlling soybean seed weight by QTL mapping and determine Dt1 , an orthologous gene of Arabidopsis TFL1 that is known to govern the soybean growth habit, as the causal gene of the SW19 locus. We showed that Dt1 is highly expressed in developing seeds and regulates photoperiod-dependent seed weight in soybean. Further analyses revealed that the Dt1 protein physically interacts with the sucrose transporter GmSWEET10a to negatively regulate the import of sucrose from seed coat to the embryo, thus modulating seed weight under long days. However, Dt1 does not function in seed development under short days due to its very low expression. Importantly, we discovered a novel natural allelic variant of Dt1 (H4 haplotype) that decouples its pleiotropic effects on seed size and growth habit; i.e., this variant remains functional in seed development but fails to regulate the stem growth habit of soybean. Collectively, our findings provide new insights into how soybean seed development responds to photoperiod at different latitudes, offering an ideal genetic component for improving soybean’s yield by manipulating its seed weight and growth habit.
研究领域:
植物学
·
遗传学
·
作物科学
✓ 已验证
DOI
PubMed
高影响因子
Foods
·
2024
·Vol. 13
·期 10
·1544
·
IF 4.7
Jingyao Yu,Yu Yang,Lanjun Luo,Fang Feng,Sana Saeed,Jie Luo,Chuanying Fang,Junjie Zhou,Kang Li
Plant factories offer a promising solution to some of the challenges facing traditional agriculture, allowing for year-round rapid production of plant-derived foods. However, the effects of conditions in plant factories on metabolic nutrients remain to be explored. In this study, we used three rice accessions (KongYu131, HuangHuaZhan, and Kam Sweet Rice) as objectives, which were planted in a plant factory with strict photoperiods that are long-day (12 h light/12 h dark) or short-day (8 h light/16 h dark). A total of 438 metabolites were detected in the harvested rice grains. The difference in photoperiod leads to a different accumulation of metabolites in rice grains. Most metabolites accumulated significantly higher levels under the short-day condition than the long-day condition. Differentially accumulated metabolites were enriched in the amino acids and vitamin B6 pathway. Asparagine, pyridoxamine, and pyridoxine are key metabolites that accumulate at higher levels in rice grains harvested from the short-day photoperiod. This study reveals the photoperiod-dependent metabolomic differences in rice cultivated in plant factories, especially the metabolic profiling of taste- and nutrition-related compounds.
研究领域:
植物学
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营养学
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农学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULAR ECOLOGY
·
2023
·Vol. 32
·期 11
·2884-2897
·
IF 4.9
Jia-Xu Li,Zhong Tian,Xing-Feng Liu,Bei Li,Hao-Min An,Colin S. Brent,Jia-Lu Wang,Xiao-Ping Wang,Wen Liu
Many animals, including insects, exhibit plasticity of body colour in response to environmental changes. Varied expression of carotenoids, major cuticle pigments, significantly contributes to body colour flexibility. However, the molecular mechanisms by which environmental cues regulate carotenoid expression remain largely unknown. In this study, we used the ladybird Harmonia axyridis as a model to investigate the photoperiodic-responsive plasticity of elytra coloration and its endocrine regulation. It was found that H. axyridis females under long-day conditions develop elytra that are much redder than those under short-day conditions, resulting from the differential accumulation of carotenoids. Exogenous hormone application and RNAi-mediated gene knockdown indicate that carotenoid deposition was directed through the juvenile hormone (JH) receptor-mediated canonical pathway. Moreover, we characterized an SR-BI/CD36 ( SCRB ) gene SCRB10 as the carotenoid transporter responding to JH signalling and regulating the elytra coloration plasticity. Taken together, we propose that JH signalling transcriptionally regulates the carotenoid transporter gene for the photoperiodic coloration plasticity of elytra in the beetles, which reveals a novel role of the endocrine system in the regulation of carotenoid-associated animal body coloration under environmental stimuli.
研究领域:
昆虫学
·
分子生物学
·
内分泌学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOLOGICAL RHYTHM RESEARCH
·
2023
·
IF 1.1
Xing-Chen Wang,Zhe Wang,Chuan-Li Wang,Hui-Liang Xue,Ming Wu,Lei Chen,Chao Fan,Jin-Hui Xu,Lai-Xiang Xu
Changes in photoperiod affect both oxidative stress (OS) levels and antioxidant enzyme activities in animals. The liver accounts for a vital body metabolic organ. Different light durations may produce different outcomes in terms of OS and liver antioxidant pathways, but the underlying mechanism is not yet understood. This study investigated the effects of diverse photoperiods (SD, short day; MD, moderate day; LD, long day) on OS degree (H2O2 and MDA), antioxidant enzyme activities (superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx]), liver total antioxidant capacity, and the Nrf2-Keap1 signaling pathway in striped dwarf hamsters (Cricetulus barabensis) livers. Our results showed that (1) SD treatment for 8 weeks increased the levels of phosphorylated Nrf2, upregulated the Nrf2-Keap1 signaling pathway, and increased the protein expression of SOD and GPx, while decreasing the MDA concentration, all of which reduced the OS degree. (2) Additionally, LD treatment for 8 weeks reduced the extent of Nrf2 phosphorylation, resulting in downregulation of the Nrf2-Keap1 pathway, thus reducing protein expression of SOD2 and CAT, increasing the concentrations of H2O2 and MDA, and increasing OS degree. Collectively, OS levels in C. barabensis liver decreased during SD but increased during LD.
研究领域:
生理学
·
分子生物学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
PLANT PHYSIOLOGY AND BIOCHEMISTRY
·
2023
·
IF 6.5
Qingwei Jia,Yuqing Yin,Shuting Gai,Lu Tian,Zhihao Zhu,Lei Qin,Yong Wang
Cryptochromes (CRYs), as blue-light photoreceptors, play a crucial role in regulating flowering time and hypocotyl and cotyledon development. Their physiological functions have been extensively studied in various plant species. However, research on onions remains limited. In this study, we identified AcCRY1 and conducted preliminary investigations into its function. Our results demonstrate that AcCRY1 possesses a conserved domain typical of cryptochromes with high homology to those found in monocots. Furthermore, we examined the expression level of AcCRY1 in onion. The green tissues is significantly higher compared to non-green tissues, and it exhibits a significant response to blue-light induction. AcCRY1 demonstrates cytoplasmic localization under blue-light conditions, while it localizes in the nucleus during darkness, indicating a strong dependence on blue-light for its subcellular distribution. In comparison to cry1 , overexpression of AcCRY1 leads to a significant shorten in seedling hypocotyl length, notable expansion of cotyledons, and acceleration of flowering time. The yeast two-hybrid experiment demonstrated the in vitro interaction between AcCRY1, AcCOP1, and AcSPA1. Additionally, BIFC analysis confirmed their interaction in Onion epidermis. Notably, under blue-light conditions, a significantly enhanced binding activity was observed compared to dark conditions. These findings establish a functional foundation for the regulatory role of AcCRY1 in important physiological processes of onion and provide initial insights into the underlying molecular mechanisms.
研究领域:
植物生物学
·
分子生物学
·
遗传学
✓ 已验证
DOI
PubMed
高影响因子
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