MICROCHIMICA ACTA
·
2019
·Vol. 187
·期 1
·1-8
·
IF 5.479
Zhang Chun-Xia,Gao Yan-Cai,Wang Chong,Yu Xu,Li Hong-Wei,Wu Yuqing
The fluorescence of adenosine monophosphate-capped bimetallic gold and silver nanoclusters (type AuAgNC@AMP) is strongly enhanced and blue shifted in the presence of Al(III). As confirmed by transmission electron microscopy, the AuAgNC nanodots are converted to larger assembled spheres of type AuAgNC-Al(III). The fluorescence enhancement is attributed to aggregation-induced emission enhancement (AIEE). The fluorescence of the AuAgNC-Al(III) assembly (with excitation and emission maxima at 340 and 540 nm) is quenched by cysteine (Cys). The effect was applied to the fluorometric determination of Cys. The assay works in the 1.0 to 16.0 μM Cys concentration range and has a 50 nM limit of detection. The method was successfully applied to analyze Cys-spiked mineral waters and serum. The quenching mechanism is explored in depth. It is attributed to the partial replacement of AMP by Cys at the surface of the AuAgNC and alteration of the assembly structure from large spherical particles to a strip shape.
Schematic representation of the fluorescence enhancement of bimetallic nanoclusters capped with adenosine monophosphate by using Al(III), and its application in selective and sensitive determination of cysteine via ligand replacement and reassembly.
研究领域:
分析化学
·
生物医学
·
纳米技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RESEARCH ON CHEMICAL INTERMEDIATES
·
2017
·Vol. 44
·期 1
·491-502
·
IF 1.369
Li Shaoxin,Zhao Bing,Kan Wei,Wang Liyan,Song Bo,Chen Shuwen
A novel pH fluorescence probe (PIF), derived from phenanthro[9,10- d ]imidazole bound to the fluorescein, is designed and synthesized based on the intramolecular hydrogen bond and then its spectra properties are investigated. Probe PIF exhibits negligible fluorescence intensity at acidic pH condition due to ESIPT quenching caused by the formation of the intramolecular hydrogen bonds, and a significant fluorescence enhancement in neutral and weak alkaline environments because of the strengthened ICT process arose from the fracture of the intramolecular hydrogen bonds. The studies of UV–Vis and fluorescence spectra mean that probe PIF is able to respond linearly and reversibly over pH from 6.5 to 9.0 with p K
a of 7.0. Moreover, the pH responses of probe PIF are stable and not disturbed by the common cations and anions. The possible response mechanism has been proposed according to the analysis of 1 H NMR and DFT calculations.
研究领域:
分析化学
·
生物化学
✓ 已验证
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Shilan A. Fatah,Khalid M. Omer
Ofloxacin (OFL), a broad-spectrum fluoroquinolone antibiotic, is widely utilized for treating bacterial infections. Accurate detection of OFL is essential for pharmaceutical quality control and environmental monitoring, particularly in wastewater. This study introduces a novel fluorescence-based detection method using sumac-derived carbon dots (S-CDs), synthesized via a simple hydrothermal approach. The S-CDs exhibited spherical morphology (20–30 nm) with blue fluorescence emission and a quantum yield of 1.4 %. Upon interaction with OFL, a remarkable fluorescence enhancement was observed, resulting in a 45-fold increase in quantum yield (45.0 %) accompanied by a red shift in the emission. This strong enhancement and red-shift emission enabled to design off–on mode for detection of OFL. The fluorescence intensity showed a strong linear correlation with OFL concentration (R 2 = 0.98) in the range of 1–90 nM, with a limit of detection (LOD) of 0.7 nM and a limit of quantification of 2.3 nM. Furthermore, a smartphone-assisted visual detection method was developed based on an off–on fluorescence mechanism. RGB analysis enabled real-time quantification of OFL, exhibiting a linear detection range of 20–80 µM with an LOD 0.97 µM. The method was successfully applied to detect OFL in eye drop formulations and tap water, demonstrating high accuracy and precision. These findings underscore the potential of S-CDs as a cost-effective, portable, and efficient tool for OFL detection, suitable for pharmaceutical and environmental monitoring applications.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF COORDINATION CHEMISTRY
·
2021
·
IF 1.751
Guo Lina,Yuanyuan Gao,Limin Han
A tridentate Schiff base probe L (2-NC5H4)C(H)=N(C6H4OH-2) was synthesized via a facile reaction. Probe L showed weak fluorescence emission that originated from intermolecular proton transfer in solution. With addition of Cu2+, the emission of L was quenched by the paramagnetic nature of Cu2+. However, after the solution of L and Cu2+ was refluxed, the emission presented a completely opposite result changing from fluorescent quenching to fluorescent enhancement. The fluorescent enhancement was attributed to a planar cis-structure of Cu2+ complex. Hence, a strategy for change of emission behavior of fluorescent probe L to Cu2+ was developed; a “turn on” mode of detection Cu2+ was realized in methanol solution. A simple refluxing made probe L work as a light-up fluorescent sensor for Cu2+ in methanol solution and gave improvement of sensitivity and selectivity in detection of Cu2+. When H2O was added into the solution of the Cu2+ complex, fluorescent enhancement increased and it can be used to detect water content in methanol.
研究领域:
化学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 48
·8461-8465
·
IF 1.915
Hongyan Wang,Jine Wang,Lijun Xu,Yajie Zhang,Yang Chen,Hongxia Chen,Renjun Pei
Aptamers are single-stranded oligonucleotides which are selected and are capable of binding certain targets tightly and specifically. Systematic Evolution of Ligands by Exponential Enrichment (SELEX) has been used to select aptamers for various targets such as proteins, metal ions, small molecules and so on. For the first time, we have used a modified SELEX technology to obtain DNA aptamers for a fluorogenic dye, thioflavin T (ThT). DNA aptamers with low micromolar dissociation constants were achieved after only 8 rounds of selection and the final minimized 33-mer aptamer showed 191-fold fluorescence enhancement in a saturated concentration. The truncated DNA aptamer showed excellent selectivity and specificity for ThT.
研究领域:
分子生物学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 7
·2275-2280
·
IF 4.58
Chen Muhua,Zheng Yuhui,Gao Jinwei,Li Chen,Yu Chufang,Wang Qianming
A hybrid organic-inorganic optical probe was developed by using silylated precursors (including tetraethoxy silane and aminopropyltriethoxysilane), ferroferric oxide (Fe 3 O 4 ) and lanthanide coordination units. Cross-linking condensation warrants chemical stability. The shape and size of the resulting nanoparticles can be adjusted to around 40–60 nm. On photo-excitation at 313 nm, they display green fluorescence (peaking at 545 nm) which is strongly enhanced on addition of dopamine in tris-buffer solution (pH = 8.5). Spectroscopic studies demonstrate that this assay exhibits good selectivity for dopamine and has a 0.82 μM detection limit. The hybrid structures can be separated and recycled due to the magnetic properties of Fe 3 O 4 . The feasibility of dopamine analysis in human serum sample has been assessed.
Schematic presentation of a terbium organic-inorganic material whose fluorescence is selectively enhanced by dopamine. The material can be repeatedly used because it can be regenerated by treatment with acids and magnetic separation.
研究领域:
分析化学
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2021
·
IF 4.539
Yang Chen,Huijing Cui,Mao Wang,Xudong Yang,Shujie Pang
Cr(VI) is a toxic heavy metal, which is not readily decomposed and ingested by bacteria. Therefore, there is an urgent need for a sensing method with high selectivity and high sensitivity to detect Cr(VI) in other widely distributed metal ions . Here, a fluorescent probe based on carbon dots (CDs) was developed, with the characteristics of low cost, low environmental impact, high stability, and good water solubility. Although many sensors have been reported for the detection of Cr(VI), most of them are based on the quenching fluorescence method , whereas the one reported here is based on fluorescence enhancement, for which o-phenylenediamine and dl -Thioctic acid were used as raw materials. The transmission electron microscopy , Fourier infrared spectroscopy , X-ray photoelectron spectroscopy, X-ray diffraction, and ultraviolet-visible spectroscopy measurements showed that Cr(VI) can significantly enhance the fluorescence of the probe compared to previous sensors, as the obtained CDs have excellent fluorescence properties (quantum yield up to 21.82%) and high light stability. The detection limit of the probe for Cr(VI) is as low as 0.64 μM, indicating that the developed probe can detect Cr(VI) quickly, sensitively, and selectively, and has broad application prospects in environmental analysis applications. To facilitate rapid detection of Cr(VI), the CDs were also incorporated into a fluorescent composite film suitable for visual inspection.
研究领域:
环境科学
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF LUMINESCENCE
·
2023
·
IF 3.6
Yanting Liu,Yanzhu Wang,Lili Meng,Qi Pang,Hua Yao,Qian Chen,Sidi Ye,Yanting Li,Minyi Zheng
Herein, we successfully obtained Ho 3+ and Ag + -codoped Cs 2 NaBiCl 6 halide double perovskites (DPs) via a hydrothermal method , which exhibit orange-red broadband self-trapped excitons (STE) emission and visible (Vis) and near-infrared (NIR) narrow band emission attributed to Ho 3+ f-f transition, under 360 nm excitation. Compared to the single Ho 3+ -doped sample, the Vis PL intensity of the codoped sample was increased by nearly 13.8 times, and an enhanced photoluminescence quantum yield (PLQY) of 87.45 % was achieved. The PL enhancement is attributed to the partial substituting of Na + with Ag + , leading to an efficient STE emission, and the more energy transition from STE to Ho 3+ . Moreover, the synthesized phosphor exhibits good stability in thermal and atmospheric environments. White light-emitting diode (WLED) is fabricated by employing Cs 2 Na 0·997 Ag 0·003 Bi 0.935 Ho 0.065 Cl 6 on near-ultraviolet (n-UV) LED chips, achieving a color rendering index (CRI) of 95.0 and correlated color temperature (CCT) of 3814 K. Anti-counterfeit patterns and the application for night vision monitoring were also discussed. This study inspires the designing of high-quality phosphors of rare earth-doped DPs and expands the multi-domain luminescence applications of halide DPs.
研究领域:
材料科学
·
光学工程
·
稀土材料
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2017
·
IF 4.162
Fangfang Li,Xuan Hu,Fengyi Wang,Baozhan Zheng,Juan Du,Dan Xiao
A simple, rapid, and ultrasensitive fluorescence strategy for adenosine triphosphate (ATP) detection was developed by using a FAM (carboxyfluorescein) labeled DNA (FAM-DNA). In this strategy, highly fluorescent FAM-DNA was used as a probe, and nanoceria (CeO 2 NPs) acted as an efficient quencher. FAM-DNA attached to the surface of nanoceria through the coordination between the phosphate group of DNA and NP surface, which induced complete quenching in the FAM-DNA fluorescence due to a photo induced electron transfer (PET) process. It was found that ATP can readily displace adsorbed DNA from nanoceria surface because of the stronger coordination ability of ATP with nanoceria, and the nanoceria-based competitive binding resulted in over 7-fold fluorescence enhancement. Over a wide range from 0.1 nM to 1.5 μM, a good linear relationship between the fluorescence intensity and the concentration of ATP was obtained and the detection limit was estimated to be as low as 54 pM. This method was successfully used to analyze ATP in a single drop of blood and human urine.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF LUMINESCENCE
·
2025
·
IF 3.6
Zhiwei Xia,Lan Yao,Xin Pan,Ke Su,Rujun Yang,Yuanhao Jian,Rong-Jun Xie,Lefu Mei
The development of efficient dual-emissive and temperature-sensitive luminescent materials is critical for advancing non-contact optical thermometry. Herein, we employed a co-doping strategy to enhance energy transfer pathways in Ca 9 Ga(PO 4 ) 7 phosphors. Under 331 nm excitation, dual emissions from Eu 2+ (415 nm) and Cr 3+ (696 nm) were achieved, with Zn 2+ enhancing the near-infrared (NIR) of Cr 3+ . The optimal Cr 3+ concentration led to an approximately six-fold increase in NIR emission intensity, attributed to Eu 2+ -induced crystal field modulation and efficient dipole-dipole energy transfer, which are evidenced by comparing the emission spectra and decay curve lifetimes. Temperature-dependent emission spectra revealed that Eu 2+ emission remained stable, while Cr 3+ emission exhibited significant temperature sensitivity in Ca 9 Ga(PO 4 ) 7 :Zn 2+ ,Eu 2+ ,Cr 3+ , enabling precise temperature sensing with a peak sensitivity of 1.256 % K −1 at 298 K. This work offers a novel approach to improving energy transfer efficiency and dual-emission properties, contributing to the development of high-performance, real-time optical thermometry and photonic technologies.
研究领域:
材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
ORGANIC & BIOMOLECULAR CHEMISTRY
·
2017
·Vol. 15
·期 28
·6006-6013
·
IF 3.564
Liangbin Lin,Xiaoru Lin,Hongyu Guo,Fafu Yang
This study focuses on the construction of novel diphenylacrylonitrile-connected BODIPY dyes with high fluorescence in both solution and an aggregated state by combining DRET and FRET processes in a single donor–acceptor system. The first BODIPY derivatives with one, two, or three AIE-active diphenylacrylonitrile groups were designed and synthesized in moderate yields. Strong fluorescence emissions were observed in the THF solution under excitation at the absorption wavelength of non-emissive diphenylacrylonitrile chromophores, implying the existence of the DRET process between the dark diphenylacrylonitrile donor and the emissive BODIPY acceptor. In the THF/H2O solution, the fluorescence intensity of the novel BODIPY derivatives gradually increased under excitation at the absorption wavelength of diphenylacrylonitrile chromophores, suggesting a FRET process between diphenylacrylonitrile and BODIPY moieties. A greater number of diphenylacrylonitrile units led to higher energy-transfer efficiencies. The pseudo-Stokes shift for both DRET and FRET processes was as large as 190 nm.
研究领域:
化学
·
材料科学
·
光学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2016
·
IF 4.035
Qiang Chen,Junfeng Zuo,Xue He,Xiujuan Mo,Ping Tong,Lan Zhang
In this paper, a simple, rapid and sensitive fluorescence method based on the formation of terbium (Tb 3+ ) complex has been developed for the rapid detection of Tb 3+ in water. The fluorescence sensor has been studied by using terbium complexed with thiabendazole (TBZ) while acetonitrile (MeCN) as solvent. The complex was made up of TBZ as small-molecule ligand and Tb 3+ as central ion. Fluorescence spectroscopy and UV spectroscopy together were used to study the behavior of the complexation of TBZ-Tb in this medium. Enhancement fluorescence was observed due to the efficient energy transfer process from TBZ to Tb 3+ . And the affecting factors of the enhancement fluorescence were also studied in detail. Under optimal conditions, a linear relationship was obtained between the enhanced fluorescence intensity and the concentration of Tb 3+ in a range of 5.0×10 −6 M–3.0×10 −5 M. The sensor was used for the detection of Tb 3+ in river water samples, and the result was satisfactory. In the meantime, a linear relationship was also obtained between the enhanced fluorescence intensity and the concentration of TBZ in a range of 8.0×10 −6 M–4.0×10 −5 M. Moreover, the method was successfully extended for the detection of TBZ in juice samples.
研究领域:
分析化学
·
环境科学
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）