JOURNAL OF CONTROLLED RELEASE
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2020
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IF 7.727
Tao Sun,Guangping Zhang,Zhongyuan Guo,Qinjun Chen,Yujie Zhang,Yongchao Chu,Qin Guo,Chao Li,Wenxi Zhou,Yiwen Zhang,Peixin Liu,Hongyi Chen,Haijun Yu,Liping Jiang,Chen Jiang
Chelating Cu from tumors has been verified as an effective and promising strategy for cancer therapy through antiangiogenesis. However, systematic removal Cu by injecting with Cu chelators will result unavoidable side effects, since Cu is indispensable to the body. In this work, a micelle targeting to tumors' newborn vessels based on a polypeptide was developed to co-load DOX and Probe X, which can go through an “OFF-to-ON” procedure to report the Cu + -capture events in vivo in a real-time way by giving near infrared (NIR) fluorescence and photoacoustic signal. By co-delivering antiangiogenesis and chemotherapeutic reagents, the tumor can be significantly suppressed, meanwhile with a low systematic toxicity. Hopefully, this work can offer new insights in designing sophisticated antitumor strategy.
研究领域:
肿瘤学
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药剂学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Liting Zhang,Xiaoxin Yan,Ting Jiang,Jie Han,Jianqiang Xiao,Kai Wang,Donglin Hao,Rui Guo,Jianhua Jin
This study introduces Hcy-GST, a new dual-modal optical probe for detecting glutathione S-transferase (GST) activity. The probe combines hemicyanine and rhodamine derivatives, which use fluorescence and photoacoustic (PA) signals, controlled by a photoinduced electron transfer (PET) mechanism. In its original form, the nitro groups in Hcy-GST quench fluorescence, producing weak fluorescence and strong PA signals. When GST replaces the nitro groups with glutathione (GSH), the PET effect is interrupted, leading to fluorescence recovery and reduced PA signals. Hcy-GST shows high sensitivity, with fluorescence and PA signals strongly correlating with GST concentration. It has high selectivity and minimal interference from other substances. In cellular tests, Hcy-GST detected GST inhibition by ethacrynic acid. In vivo studies revealed lower GST activity in obese models, with PA signals increasing after probe injection. Hcy-GST demonstrated excellent stability and low toxicity, showing promise for GST detection in research and diagnostics.
研究领域:
生物化学
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医学诊断
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DOI
高影响因子
ANALYTICA CHIMICA ACTA
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2020
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IF 5.977
Ya-Jie Zhang,Lan Guo,Shuai Chen,Yong-Liang Yu,Jian-Hua Wang
It is still a high challenge to develop a simple, sensitive and portable approach for bioassay in strong scattering medium. Herein, a photoacoustic (PA) device is developed for the detection of alkaline phosphatase (ALP) in serum with silver nanoparticles (AgNPs) as signal probe, without any requirements for expensive equipment, professional operation and pre-processing of real samples. ALP as an important disease marker could catalyze the breakdown of sodium L-ascorbyl-2-phosphate (AAP) into ascorbic acid (AA), thereby reducing Ag + to AgNPs. AgNPs could generate strong PA signal under the irradiation of modulated 638-nm laser due to their localized plasmon resonance, and detected by the self-made portable PA device. Under the optimized experimental conditions, the present PA device exhibits excellent photostability and reproducibility with the relative standard deviation (RSD) of 2.2% at the concentration of 25 U L −1 ALP. Linear calibration graph is obtained within 5–70 U L −1 for ALP, along with a detection limit of 1.1 U L −1 . This portable PA device is applied to detect ALP in serum samples, providing satisfactory spiking recoveries and competitive analytical performances with the current techniques. The PA-based analytical strategy obviously opens up a new avenue to the detection of disease-correlated biomarker in practice.
研究领域:
生物化学
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分析化学
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医学诊断
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产品关联
DOI
PubMed
高影响因子
Acta Biomaterialia
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2020
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IF 7.242
Ying Wang,Xiaowen Xu,Xuan Zhao,Zongning Yin
Pathological thrombosis within a vessel hampers blood flow and is the mainspring of numerous fatal cardiovascular complications. In order to specifically image and dissolve a thrombus, we rationally designed a functionalized polymeric hybrid micelle (PHM) system self-assembled from amphiphilic polycaprolactone-polyethylenimine (PCL-PEI) and polycaprolactone-polyethylene glycol (PCL-PEG). Based on a biological component of thrombi, activated coagulation factor XIII (FXIIIa), which is responsible for fibrin crosslinking, we further developed FXIIIa-targeted near infrared imaging and thrombolytic nanoparticles , termed IR780/FPHM/LK NPs, through chemical conjugation of peptides to the system. In a ferric chloride (FeCl 3 )-induced mouse carotid thrombosis model, IR780/FPHM/LK NPs specifically targeted the thrombus and significantly enhanced the photoacoustic signal for an accurate diagnosis. When loaded with the fibrinolytic drug lumbrokinase (LK), FPHM remarkably dissociated the thrombus accompanied by an increase in the d -dimer level, a fibrin degradation product , and alleviation of fatal nonspecific hemorrhagic risk. Given its thrombus-specific imaging along with potent therapeutic activities, IR780/FPHM/LK NPs hold promise for developing nanotheranostic agents in preclinical thrombotic vascular disease models.
研究领域:
纳米技术
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诊疗学
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心血管疾病
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药学
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生物医学工程
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产品关联
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
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2019
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IF 8.456
Boshu Ouyang,Funan Liu,Shuangrong Ruan,Yao Liu,Huishu Guo,Zhengxu Cai,Xiangrong Yu,Zhiqing Pang,Shun Shen
As a novel treatment modality of tumors, hypothermal hyperthermia employed relatively lower temperature (<45 °C) to damage cancer cells with mild toxicity to normal tissues. However, beyond that inducible heat resistance of tumor cells, the discounted therapeutic effect of low temperature hyperthermia was also ascribed to poor penetration of exogenous light stimulation and low accumulation of photothermal agents in tumor sites. Herein, we constructed a multifunctional in situ hydrogel of sodium alginate (ALG) via Ca2+ coordinated with ALG to encapsulate the photothermal agent of Ink and azo initiator of 2,2′-azobis[2-(2-imidazolin-2-yl)propane]dihydrochloride (AIPH) for effective tumor treatment. The designed ALG hydrogel was used to improve the therapeutic effect by increased accumulation of Ink and AIPH and avoid potential side-effects caused by the unexpected spread to the surrounding normal tissues. After injection, local low temperature stimulation was generated with near-infrared-II irradiation by a 1064 nm laser, triggering rapid decomposition of AIPH to produce alkyl radicals. The synergistic low temperature photothermal therapy and cytotoxic-free radicals enhanced the apoptosis of tumor cells via physical heat damage and lipid peroxidation. Thus, remarkable inhibition of tumor growth was observed in a subcutaneous colorectal cancer with negligible side effects. Furthermore, the formulation could also exert strong photoacoustic signals, which were utilized to monitor the stability of the composite hydrogel.
研究领域:
生物医学工程
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癌症治疗
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光热疗法
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材料科学
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产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
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2017
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IF 8.402
Wen Gao,Shuangshuang Li,Zhenhua Liu,Yuhui Sun,Wenhua Cao,Lili Tong,Guanwei Cui,Bo Tang
Attacking the supportive vasculature network of a tumor offers an important new avenue for cancer therapy. Herein, a near-infrared (NIR) laser-activated “nanobomb” was developed as a noninvasive and targeted physical therapeutic strategy to effectively disrupt tumor neovasculature in an accurate and expeditious manner. This “nanobomb” was rationally fabricated via the encapsulation of vinyl azide (VA) into c(RGDfE) peptide-functionalized, hollow copper sulfide (HCuS) nanoparticles . The resulting [email protected] (VA) was selectively internalized into integrin α v β 3 -expressing tumor vasculature endothelial cells and dramatically increased the photoacoustic signals from the tumor neovasculature, achieving a maximum signal-to-noise ratio at 4 h post-injection. Upon NIR irradiation, the local temperature increase triggered VA to release N 2 bubbles rapidly. Subsequently, these N 2 bubbles could instantly explode to destroy the neovasculature and further induce necrosis of the surrounding tumor cells. A single-dose injection of [email protected] (VA) led to complete tumor regression after laser irradiation , with no tumor regrowth for 30 days. More importantly, high-resolution photoacoustic angiography, combined with excellent biodegradability , facilitated the precise destruction of tumor neovasculature by [email protected] (VA) without damaging normal tissues. These results demonstrate the great potential of this “nanobomb” for clinical translation to treat cancer patients with NIR laser-accessible orthotopic tumors.
研究领域:
癌症研究
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纳米技术
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生物医学工程
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诊疗学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
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2021
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IF 5.221
Wu Mingzhou,He Shuqing,Hu Xin,Chen Jingqin,Ha Enna,Ai Fujin,Ji Tao,Hu Junqing,Ruan Shuangchen
Transition-metal chalcogenide compounds with facile preparation and multifunctional elements act as ideal photothermal agents for cancer theranostics. This work synthesizes Cu7.2S4/5MoS2 composite nanoflowers and investigates the crystal growth mechanism to optimize the synthesis strategy and obtain excellent photothermal therapy agents. Cu7.2S4/5MoS2 exhibits a high photothermal conversion efficiency of 58.7% and acts as a theranostic nanoplatform and demonstrated an effective photothermal–chemodynamic–photodynamic synergetic therapeutic effect in both in vitro and in vivo tests. Moreover, Cu7.2S4/5MoS2 shows strong photoacoustic signal amplitudes and computed tomographic contrast enhancement in vivo. These results suggest a potential application of Cu7.2S4/5MoS2 composite nanoflowers as photo/H2O2-responsive therapeutic agents against tumors.
研究领域:
纳米技术
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癌症诊疗
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生物医学工程
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材料科学
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医学成像
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nature Communications
·
2024
·Vol. 15
·期 1
·1-24
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IF 14.7
Song Jianwen,Wang He,Meng Xue,Li Wen,Qi Ji
Activatable theranostic systems combining precise diagnosis and robust immune activation have significant potential in cancer treatment. Herein, we develop a versatile nanoplatform integrating hypoxia-activatable molecular imaging with effective photoimmunotherapy for cancer treatment. Our molecular probe features turn-on near-infrared-II (NIR-II) fluorescence and photoacoustic signals in hypoxic tumor environments. It also induces hypoxia-triggered photodynamic and photothermal effects, promoting immunogenic cell death and activating the STING pathway, engaging both innate and adaptive immunity. The molecular probe is formulated with a vascular disrupting agent to amplify the hypoxia-responsive phototheranostic properties, on which M1-like macrophage membrane is camouflaged to shield against premature release while conferring cancer-targeting affinity. The activatable NIR-II fluorescence and photoacoustic imaging enable precise tumor delineation, while the enhanced phototherapy activates tumor-specific cytotoxic T cells, impeding both primary and distant tumor progression and providing protective immunity against rechallenge in 4T1 tumor-bearing female mice. This work advances activatable theranostic protocols for image-guided immunotherapy.
研究领域:
癌症治疗
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医学成像
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免疫学
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纳米技术
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生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）