Advanced Science
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2021
·Vol. 8
·期 16
·2100228
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IF 16.806
Jun Wang,Tingting Li,Jen-Shyang Ni,Heng Guo,Tianyi Kang,Zeshun Li,Menglei Zha,Songbo Lu,Chen Zhang,Weizhi Qi,Lei Xi,Kai Li
Precise and efficient delivery of nanomedicine to the target site has remained as a major roadblock in advanced cancer treatment. Here, a novel photoacoustic force (PAF)-guided nanotherapeutic system is reported based on a near-infrared (NIR)-absorbing semiconducting polymer (SP), showing significantly improved tumor accumulation and deep tissue penetration for enhanced phototherapeutic efficacy. The accumulation of nanoparticles in 4T1 tumor-bearing mice induced by the PAF strategy displays a fivefold enhancement in comparison with that of the traditional passive targeting pathway, in a significantly shortened time (45 min vs 24 h) with an enhanced penetration depth in tumors. Additionally, a tumor-bearing mouse model is rationally designed to unveil the mechanism, indicating that the nanoparticles enter solid tumors through enhanced transportation across blood vessel barriers via both inter-endothelial gaps and active trans-endothelial pathways. This process is specifically driven by PAF generated from the nanoparticles under NIR laser irradiation. The study thus demonstrates a new nanotherapeutic strategy with low dose, enhanced delivery efficiency in tumor, and boosted therapeutic efficacy, opening new doors for designing novel nanocarriers.
研究领域:
生物医学工程
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癌症治疗
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纳米医学
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产品关联
DOI
PubMed
高影响因子
Journal of Physical Chemistry C
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2021
·
IF 4.126
Yichun Peng,Shenghua Ma,Zhihua Shao,Qiangzhou Rong
Photoacoustic imaging is a new noninvasive imaging technology that combines the advantages of optical imaging and ultrasound imaging. Using materials with good photoacoustic effects can improve imaging contrast and resolution. With the development of photoacoustic imaging in the field of medical treatment, research pertaining to photoacoustic functional materials is progressing continuously. In this study, we effectively synthesize a multifunctional photoacoustic and magnetocaloric nanomaterial, i.e., SiO2/C/Fe3O4 via a one-step synthesis method. For theoretical analysis, the finite elemental analysis method is used to simulate the photoacoustic and magnetocaloric signals of the material. To obtain the experimental results, the SiO2/C/Fe3O4 suspension is excited by a 532 nm nanosecond pulsed laser irradiation to obtain the photoacoustic signal of the material. The temperature increase of the material is measured under a 365 kHz alternating magnetic field. The results show that SiO2/C/Fe3O4 exhibits good photoacoustic and magnetocaloric effects, rendering it feasible as a contrast agent for photoacoustic imaging and magnetocaloric therapy.
研究领域:
医学影像学
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材料科学
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生物医学工程
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产品关联
DOI
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-14
·
IF 4.259
Huang Zebin,Xu Jiankun,Chen Jiebin,Chen Hongjiang,Wang Hailong,Huang Zhonglian,Chen Youbin,Lu Xiaolin,Lu Fushen,Hu Jun
The aim of this study was to evaluate the direct photoacoustic (PA) effect on bone marrow mesenchymal stem cells (BMSCs) which is a key cell source for osteogenesis. As scaffold is also an indispensable element for tissue regeneration, here we firstly fabricated a composited sheet using polylactic-co-glycolic acid (PLGA) mixing with graphene oxide (GO). BMSCs were seeded on the PLGA-GO sheets and received PA treatment in vitro for 3, 9 and 15 days, respectively. Then the BMSCs were harvested and subjected to assess alkaline phosphatase (ALP) activity, calcium content and osteopontin (OPN) on 3, 9 and 15 days. For in vivo study, PLGA-GO sheet seeded with BMSCs after in vitro PA stimulation for 9 days were implanted to repair the bone defect established in the femoral mid-shaft of Sprague-Dawley rat. PLGA-GO group with PA pretreatment showed promising outcomes in terms of the expression of ALP, OPN, and calcium content, thus enhanced the repair of bone defect. In conclusion, we have developed an alternative approach to enhance the repair of bone defect by making good use of the beneficial effect of PA.
研究领域:
生物医学工程
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再生医学
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组织工程
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DOI
PubMed
高影响因子
Acta Biomaterialia
·
2024
·
IF 9.4
Kunlin Li,Lin Li,Xiyue Xie,Jing Zhu,Daqing Xia,Lunli Xiang,Kaiyong Cai,Jixi Zhang
Although immunogenic cell death (ICD) induced by lysosomal membrane permeabilization (LMP) evidently enhance the effectiveness of antitumor immunity for triple-negative breast cancer (TNBC) with poor immunogenicity, their potential is increasingly restricted by the development of other death pathways and the repair of lysosomes by endoplasmic reticulum (ER) during LMP induction. Herein, a polydopamine nanocomposite with i-motif DNA modified and BNN6 loaded is prepared toward boosting LMP and immunotherapy of TNBC by synergy of spatially confined photoacoustic (PA) effects and nitric oxide. Combining the high-frequency pulsed laser (4000 kHz) with the intra-lysosomal assembly of nanocomposites produced spatially confined and significantly boosted PA effects (4.8-fold higher than the individually dispersed particles extracellular), suppressing damage to other cellular components and selectively reducing lysosomal integrity to 19.2%. Simultaneously, the releasing of nitric oxide inhibited the repair of lysosomes by ER stress, causing exacerbated LMP. Consequently, efficient immune activation was achieved, including the abundant releasing of CRT/HMGB1 (5.93–6.8-fold), the increasing maturation of dendritic cells (3.41-fold), and the fostered recruitment of CD4 + /CD8 + T cells (3.99–3.78-fold) in vivo. The study paves a new avenue for the rational design and synergy of confined energy conversion and responsive nanostructures to achieve the treatment of low immunogenicity tumors. Statement of Significance A strategy of boosting lysosomal membrane permeabilization (LMP) and concomitantly preventing the repair was developed to address the immunotherapy challenge of triple-negative breast cancer. Spatially confined and significantly enhanced photoacoustic (PA) effects were achieved through DNA-guided pH-responsive assembly of polydopamine nanocomposites in lysosomes and application of a high-frequency pulsed laser. Efficient immunogenic cell death was guaranteed by selective and powerful damage of lysosomal membranes through the significant contrast of PA intensities for dispersed/assembled particles and nitric oxide release induced endoplasmic reticulum stress. The study paves a new avenue for the rational design and synergy of confined energy conversion and responsive nanostructures to achieve the treatment of low immunogenicity tumors.
研究领域:
癌症生物学
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纳米技术
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免疫治疗
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生物医学工程
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光声成像
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产品关联
DOI
PubMed
高影响因子
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