ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Chaobang Zhang,Zhidong Qiu,Liangliang Zhang,Shulong Wang,Shulin Zhao,Qiufang Pang,Hong Liang
The multispectral optoacoustic tomography (MSOT) technique can be used to perform high-resolution molecular imaging under deep tissues, which gives the technology significant prospective for clinical application. Here, we developed a superoxide anion (O2•–)-activated MSOT and fluorescence dual-modality imaging probe (APSA) for early diagnosis of drug-induced liver injury (DILI). APSA can respond quickly to O2•–, resulting in an absorption peak blueshift from 845 to 690 nm, which also leads to the photoacoustic (PA) signal at 690 nm and the fluorescence signal at 748 nm increases linearly with increasing O2•– concentration, which can be utilized to assess the extent of liver damage. The developed MSOT imaging method can eliminate background interference from hematopoietic tissue by collecting the PA signals excited at 680, 690, 740, 760, 800, 845, and 900 nm wavelengths to achieve noninvasive in situ visual diagnosis of DILI. The developed fluorescence imaging method can be used for the imaging of endogenous O2•– in living cells and anatomic diagnosis of liver injury. The developed probe has broad application prospects in the early diagnosis of DILI.
研究领域:
生物医学工程
·
肝脏病学
·
分子影像学
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2020
·
IF 5.977
Ya-Jie Zhang,Lan Guo,Shuai Chen,Yong-Liang Yu,Jian-Hua Wang
It is still a high challenge to develop a simple, sensitive and portable approach for bioassay in strong scattering medium. Herein, a photoacoustic (PA) device is developed for the detection of alkaline phosphatase (ALP) in serum with silver nanoparticles (AgNPs) as signal probe, without any requirements for expensive equipment, professional operation and pre-processing of real samples. ALP as an important disease marker could catalyze the breakdown of sodium L-ascorbyl-2-phosphate (AAP) into ascorbic acid (AA), thereby reducing Ag + to AgNPs. AgNPs could generate strong PA signal under the irradiation of modulated 638-nm laser due to their localized plasmon resonance, and detected by the self-made portable PA device. Under the optimized experimental conditions, the present PA device exhibits excellent photostability and reproducibility with the relative standard deviation (RSD) of 2.2% at the concentration of 25 U L −1 ALP. Linear calibration graph is obtained within 5–70 U L −1 for ALP, along with a detection limit of 1.1 U L −1 . This portable PA device is applied to detect ALP in serum samples, providing satisfactory spiking recoveries and competitive analytical performances with the current techniques. The PA-based analytical strategy obviously opens up a new avenue to the detection of disease-correlated biomarker in practice.
研究领域:
生物化学
·
分析化学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOMATERIALS
·
2021
·
IF 12.479
Ruijing Zhang,Qin Zeng,Xipeng Li,Da Xing,Tao Zhang
Current light-mediated photodynamic therapy (PDT) is far underutilized in clinical cancer treatment due to its low pharmacological effect . We herein proposed a new gadolinium(III)-phthalocyanine (GdPc)-enabled phototherapeutics, photoacoustic/dynamic therapy (PADT), towards in vivo solid tumors via parallel-produced photocavitation and photodynamic oxidation with excitation by a single pulsed laser . We demonstrated that pulsed irradiation of GdPc could simultaneously produce an intense acoustic effect and a high-level 1 O 2 quantum yield to afford mitochondrial damage and initiate programmed cell death. Under the guidance of magnetic resonance/photoacoustic dual-modal imaging, the mechanical oxygen-independent destruction of acoustic cavitation and the chemical damage of 1 O 2 were validated to afford combinatorial inhibition of tumors under either normal or hypoxic conditions after the agent delivered into the cancer cells by a pH-sensitive nanomicelle. The single-laser initiated PADT using GdPc as a versatile photoagent maximizes the use of light energy to minimize the dose requirement of oxygen and agent towards high therapeutic efficacy, surpassing dramatically over conventional PDT.
研究领域:
诊疗学
·
医学成像
·
光动力治疗
·
肿瘤学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2019
·
IF 3.206
Qiaozhi He,Qian Zhang,Wenwu Cao,Ting Yin,Siwei Zhao,Xiaoqia Yin,Hui Zhao,Wei Tao
A gold nanospheres (Au NSs)-enhanced photoacoustic (PA) system has been developed to detect parts per trillion (ppt)-level mercury ion (Hg 2+ ) in aqueous environments, which combines the advantages of the high sensitivity of PA detection and high extinction coefficient of Au NSs. 4-mercaptophenylboronic acid (MPBA) is used as the aggregation agent to detect Hg 2+ by a designed PA system. Experimental results demonstrate a linear relationship between the amplitude of PA signals and logarithmic concentration of Hg 2+ in the range of 10 pM-1 μM (R=0.994). The detection limit of this Au NSs-enhanced PA system is 78 pM (16 ppt, S/N=3) with a quick response time of 1 min. This method has excellent selectivity for Hg 2+ over other heavy metal ions , alkali, and alkaline earth metal ions . The system is cost-effective and shows a great potential for quick detection of Hg 2+ trace in water with very high sensitivity.
研究领域:
环境科学
·
分析化学
✓ 已验证
DOI
高影响因子
Nature Communications
·
2021
·Vol. 12
·期 1
·1-9
·
IF 14.919
Du Xinyuan,Li Jiapu,Niu Guangda,Yuan Jun-Hui,Xue Kan-Hao,Xia Mengling,Pan Weicheng,Yang Xiaofei,Zhu Benpeng,Tang Jiang
Lead halide perovskites have exhibited excellent performance in solar cells, LEDs and detectors. Thermal properties of perovskites, such as heat capacity and thermal conductivity, have rarely been studied and corresponding devices have barely been explored. Considering the high absorption coefficient (10 4 ~10 5 cm −1 ), low specific heat capacity (296–326 J kg −1 K −1 ) and small thermal diffusion coefficient (0.145 mm 2 s −1 ), herein we showcase the successful use of perovskite in optoacoustic transducers. The theoretically calculated phonon spectrum shows that the overlap of optical phonons and acoustic phonons leads to the up-conversion of acoustic phonons, and thus results in experimentally measured low thermal diffusion coefficient. The assembled device of PDMS/MAPbI 3 /PDMS simultaneously achieves broad bandwidths (−6 dB bandwidth: 40.8 MHz; central frequency: 29.2 MHz), and high conversion efficiency (2.97 × 10 −2 ), while all these parameters are the record values for optoacoustic transducers. We also fabricate miniatured devices by assembling perovskite film onto fibers, and clearly resolve the fine structure of fisheyes, which demonstrates the strong competitiveness of perovskite based optoacoustic transducers for ultrasound imaging.
研究领域:
材料科学
·
医学影像
·
光学
✓ 已验证
DOI
PubMed
高影响因子
Theranostics
·
2019
·
IF 8.063
Duyang Gao,Boyu Zhang,Yubin Liu,Dehong Hu,Zonghai Sheng,Xuanjun Zhang,Zhen Yuan
Background : Engineering a single organic-molecule-based nanoparticle integrating precise diagnosis and effective therapy is of great significance for cancer treatment and future clinical applications but remains a great challenge. The goal of this study is to explore small organic molecule-based nanoparticles with high photothermal conversion efficiency for photoacoustic imaging-guided therapy. Methods : Heptacyclic B, O-chelated BODIPY structure (namely Boca-BODIPY) with strong near-infrared (NIR) absorption was designed as a theranostic agent through simply molecular engineering, in which heavy atoms and alkyl chains were introduced to promote its application for tumor theranostics. The Boca-BODIPY molecules are further encapsulated in reduced bovine serum albumin (BSA) through self-assembly. Results : The BSA-Boca-BODIPY exhibited excellent biocompatibility, extraordinary stability and high photothermal conversion efficiency up to 58.7%. The nanoparticles could dramatically enhance photoacoustic contrast of the tumor region, and the signal-to-noise ratio was increased about 14 times at 10 h post intravenous injection in 4T1 tumor-bearing mice. In addition, the nanoassemblies can efficiently convert laser energy (808 nm, 0.75 w cm -2 , 5min) into hyperthermia for tumor ablation. Under the photoacoustic imaging-guided photothermal therapy (PTT), the 4T1 cancer cells were efficiently killed, no tumor recurrence and PTT-induced toxicity is observed. Conclusions : Molecular engineering is a promising way to design organic-molecule-based nanoparticles for cancer theranostics. Other organic-molecule-based nanoparticles which show great promise for imaging-guided cancer precision therapy can be engineered through this method.
研究领域:
生物医学工程
·
癌症治疗学
·
分子影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2020
·Vol. 31
·期 10
·2009314
·
IF 16.836
Caina Xu,Yanbing Wang,Erlei Wang,Nan Yan,Shu Sheng,Jie Chen,Lin Lin,Zhaopie Guo,Huayu Tian,Xuesi Chen
Exploiting a comprehensive strategy that processes diagnosis and therapeutic functions is desired for eradicating tumors. In this study, two versatile nanoparticles are introduced: one is polyethylene glycol- and polyethyleneimine-modified gold nanorods (mPEG–PEI–AuNRs), and the other is formed by electrostatic interactions between mPEG–PEI and calcium carbonate nanoparticles (mPEG–PEI/CaNPs). These two nanoparticles possess following favorable properties: 1) mPEG–PEI–AuNRs and mPEG–PEI/CaNPs show not only high cell uptake in acidic tumoral pH, but also efficient accumulation in tumors with prolonged circulation. 2) mPEG–PEI/CaNPs can generate carbon dioxide (CO 2 ) bubbles in acidic tumoral environment and the photoacoustic (PA) signals from mPEG–PEI–AuNRs can be enhanced with the generation of CO 2 bubbles. 3) The tumors can be eradicated by combining photothermal therapy (PTT) with ultrasonic therapy (UST) under the near-infrared (NIR) laser and ultrasonic irradiation with the presence of mPEG–PEI–AuNRs and CO 2 bubbles from mPEG–PEI/CaNPs. The detailed evaluation of cellular uptake, photothermal property of mPEG–PEI–AuNRs, CO 2 bubbles’ generation from mPEG–PEI/CaNPs, imaging, and combined PTT and UST are carried out in vitro or in vivo. This work has great potential usage for diagnosis and treatment in the future.
研究领域:
生物医学工程
·
肿瘤学
·
纳米医学
✓ 已验证
DOI
高影响因子
ACS Omega
·
2019
·
IF 2.584
Kaiwen Shen,Yuting Huang,Qiuju Li,Min Chen,Limin Wu
Gold-based nanomaterials have attracted extensive interest for potential application in photothermal therapy (PTT) owing to their distinctive properties including high photothermal transduction, biocompatibility, and low cytotoxicity. Herein, assembled gold nanoparticle architecture-based photothermal conversion agents were synthesized by using polysaccharides (alginate dialdehyde, ADA) as both the cross-linker to induce self-assembly of diphenylalanine (FF) and the reducer for in situ reduction of Au3+ ions into Au nanoparticles (Au NPs). The extinction spectrum of the obtained self-assembled ADA–FF/Au nanospheres was finely modulated into a near-infrared region by controlling the growth of Au NPs inside the assemblies. The strong plasmonic coupling effect of the assembled Au NPs also leads to high photothermal conversion (η = 40%) of the ADA–FF/Au nanospheres, hence presenting good performance in PTT and photoacoustic imaging. This synthesis technique is promising to construct nanomaterials with desired functions for potential biomedical application by self-assembly of various nanocrystals in situ.
研究领域:
生物医学工程
·
材料科学
·
纳米医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Xialing Qin,Fan Li,Yifan Zhang,Gongcheng Ma,Tao Feng,Yongxiang Luo,Peng Huang,Jing Lin
Photoacoustic detection is an emerging noninvasive and nonionizing detection technique with the merits of rich contrast, high resolution, and deep tissue penetration, especially for in vivo detection and imaging. Herein, we developed a photoacoustic (PA) molecular imaging probe (denoted as nanonaps) composed of a naphthalocyanine dye and a heptamethine dye as the internal standard with unchanged signals at 860 nm and the sensing component with peroxynitrite (ONOO–) target-decreased signals at 775 nm, respectively. The as-prepared nanonaps displayed high sensitivity and specificity of ONOO– both in vitro and in vivo. The PA860/PA775 ratio was increased as a function of the concentration of ONOO– (0–250 nM). More interestingly, our ratiometric nanonaps could be used for in vivo detection and imaging of ONOO–.
研究领域:
生物医学工程
·
分子影像学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Innovative Optical Health Sciences
·
2022
·
IF 2.396
Jiayue Zhao,Fei Gong,Nailin Yang,Huali Lei,Zhihui Han,Yuqi Yang,Liang Cheng
Photoacoustic (PA) imaging with much deeper tissue penetration and better spatial resolution had been widely employed for the prevention and diagnosis of many diseases. In this study, a new type of hydrogen peroxide (H 2 O 2 ) -activated photoacoustic nanoprobe [Mn-AH nanoscale coordination polymer nanodots (NCPs)] was successfully synthesized by a simple one-step method in water phase containing 2,2 ′ -azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), horse radish peroxidase (HRP), and manganese ion (Mn 2 + ) . After modification by polyethylene glycol (PEG), Mn-AH NCPs exhibited excellent stability and biocompatibility for in vivo H 2 O 2 -responsive chromogenic assay with great specificity and sensitivity. In the presence of H 2 O 2 , colorless ABTS would be converted by HRP into the oxidized form with strong near-infrared (NIR) absorbance, enabling photoacoustic detection of endogenous H 2 O 2 . Using H 2 O 2 -activated Mn-AH NCPs, we have successfully performed PA imaging and H 2 O 2 detection of subcutaneous murine colon CT26 tumor and deep-seated orthotopic bladder tumor. Due to the inherent Mn element existence inside the Mn-AH, this nanoprobe also serves as a good T1-weighted magnetic resonance imaging (MRI) contrast agent simultaneously. Lastly, after accomplishing its imaging functions, the Mn-AH NCPs could be cleared out from the body without any long-term toxicity, providing a new opportunity for cancer diagnosis and treatment.
研究领域:
生物医学工程
·
肿瘤学
·
影像医学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Healthcare Materials
·
2023
·
IF 10
Xianbao Sun,Lingling Xu,Hai-Dong Xu,Limin Xie,Rui Wang,Zhimou Yang,Wenjun Zhan,Shurong Shen,Gaolin Liang
Current molecular photoacoustic (PA) probes are designed with either stimulus-turned “on” or assembly-enhanced signals to trace biological analytes/events. PA probes based on the nature-derived click reaction between 2-cyano-6-aminobenzothiazole (CBT) and cysteine (Cys) (i.e., CBT-Cys click reaction) possess both “turn-on” and “enhanced” PA signals; and thus, should have higher sensitivity. Nevertheless, such PA probes, particularly those for sensitive imaging of tumor hypoxia, remain scarce. Herein, a PA probe NI-Cys(StBu)-Dap(IR780)-CBT (NI-C-CBT) is rationally designed, which after being internalized by hypoxic tumor cells, is cleaved by nitroreductase under the reduction condition to yield cyclic dimer C-CBT-Dimer to turn the PA signal “ON” and subsequently assembled into nanoparticles C-CBT-NPs with additionally enhanced PA signal (“Enhanced”). NI-C-CBT exhibits 1.7-fold “ON” and 3.2-fold overall “Enhanced” PA signals in vitro. Moreover, it provides 1.9-fold and 2.8-fold overall enhanced PA signals for tumor hypoxia imaging in HeLa cells and HeLa tumor-bearing mice, respectively. This strategy is expected to be widely applied to design more “smart” PA probes for sensitive imaging of important biological events in vivo in near future.
研究领域:
生物医学工程
·
分子影像学
·
肿瘤学
✓ 已验证
DOI
PubMed
高影响因子
MOLECULAR PHARMACEUTICS
·
2020
·
IF 4.321
Yan Ma,Dong-Yang Zhang,Zhuo Peng,Shixia Guan,Junqiu Zhai
Combinational administration of photothermal therapy (PTT) and chemotherapy (CT) shows great potential in improving the efficiency of tumor treatment. Herein, we designed a novel nanocomposite Pt@Bi2Te3 composed of bismuth telluride (Bi2Te3) nanoparticles and platinum(IV) prodrugs (Pt) for PTT–CT combination therapy. The obtained Bi2Te3 was synthesized by a simple solvothermal method and modified by polyethylene glycol, which exhibited excellent photothermal (PT) efficiency and stability and could also serve as a bimodal bioimaging contrast agent in PT and photoacoustic (PA) imaging. In vitro experiment results showed that the nanocomposite Pt@Bi2Te3 could effectively increase the uptake of platinum in cancer cells, which could kill tumor cells through the combined effect of PTT and CT. Furthermore, combination therapy of cancer in vivo was achieved with obvious tumor-growth inhibition without inducing observed side effects. We revealed the great potential of Bi2Te3 nanocomposites in increasing therapeutic efficiency by PTT–CT therapy and PA–PT imaging.
研究领域:
肿瘤学
·
生物医学工程
·
材料科学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）