Biomedical Optics Express
·
2024
·Vol. 15
·期 7
·4253-4263
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IF 2.9
Xiaowei Zhao,Wenli Liu,Zhixiong Hu,Liangcheng Duan,Xiao Zhang,Fei Li,Baoyu Hong
Retinal vascular health holds paramount importance for healthy vision. Many technologies have been developed to examine retinal vasculature non-destructively, including fundus cameras, optical coherence tomography angiography (OCTA), fluorescein angiography (FA), and so on. However, there is a lack of a proper phantom simulating the critical features of the real human retina to calibrate and evaluate the performance of these technologies. In this work, we present a rapid, high-resolution, and economical technology based on 3D printed mold-based soft lithography and spin coating for the fabrication of a multivascular network and multilayer structural retinal phantom with the appropriate optical properties. The feasibility of the retinal phantom as a test device was demonstrated with an OCTA system and a confocal retinal ophthalmoscope. Experiment results prove that the retinal phantom could provide an objective evaluation of the OCTA and confocal retinal ophthalmoscope. Furthermore, the microfluidic phantoms enabled by this fabrication technology may support the development and evaluation of other techniques.
研究领域:
医学影像
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生物工程
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眼科研究
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DOI
PubMed
高影响因子
ACS Nano
·
2025
·
IF 16
Modong Jiang,Lijie Qu,Yuan Tao,Jincheng Ni,Yanlei Hu,Dong Wu,Jiaru Chu,Jiawen Li
Transparent ceramics face fabrication challenges in achieving high-resolution 3D architectures with optical isotropy and thermal/mechanical stability. While two-photon lithography (TPL) enables nanoscale 3D printing, existing inorganic TPL materials suffer from opacity, big shrinkage, and costly postprocessing. Here, we report a solvent-free, high-loading Mg-cross-linked photoresist for TPL fabrication of sub-200 nm 3D MgO transparent ceramics with high fidelity via two-step sintering. With a high-purity cubic lattice, defect-free and fully dense MgO structures exhibit a wide range of transparent thicknesses. Demonstrations include excellent optical imaging, focusing capabilities, and stable mechanical and optical durability of MgO under various harsh working conditions, like high temperature and high-energy laser radiation. This simple and relatively low-cost method also showcases a fiber-integrated micro-optic device for high-intensity laser systems. This synergy of nanoscale precision, wide-thickness transparency, and outstanding stability will position 3D MgO transparent ceramics as next-generation optical components to make micro-optical devices with high damage thresholds.
研究领域:
材料科学
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光学工程
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纳米技术
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产品关联
DOI
PubMed
高影响因子
Sustainability
·
2023
·Vol. 15
·期 9
·7090
·
IF 3.9
Ze-Jun Wang,Yu-Hang Zhang,Rong-Yao Gao,Hua-Bing Jia,Xiao-Jing Liu,Ya-Wen Hu,Qian-Qian Shao,Li-Min Fu,Jian-Ping Zhang
This work reports the biological toxicity of nano plastic particles (NPs) to silkworms fed on the bait dopped with polystyrene encapsulated luminescent nanoparticles. The processes of NPs intake and excretion were monitored by means of time-gated optical imaging (TGI) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS), which allowed the quantification of the spatiotemporal deposition of NPs in an individual silkworm. The rates of NPs excretion and sequestration were found to be 99.92% and 0.08%, respectively, and the NPs retentate stayed mainly in the fat body (67.7%), digestive tract (18.0%), and head (7.54%). Adverse effects of NPs exposure were accordingly confirmed such as growth retardation and smaller physique. The results of the present work confirmed the possibility of nano-plastics accumulating and transmitting along the food chain in terrestrial ecosystems. The present work demonstrates the potential of employing silkworm as a model of full metamorphosed insects for exploring the biological impact of NPs on congeneric terrestrial animals, as well as the efficacy of the TGI-MS modality for in situ visualizing and quantifying the propagation of NPs via the primary food chain.Keywords:nano plastic particles;time-gated optical imaging;silkworm
研究领域:
环境科学
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毒理学
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DOI
高影响因子
ANALYST
·
2016
·Vol. 141
·期 18
·5366-5373
·
IF 4.033
Jie Tang,Yingying Su,Dongyan Deng,Lichun Zhang,Na Yang,Yi Lv
Using probes based on persistent luminescence nanoparticles (PLNPs) is a promising methodology for direct imaging analysis of bio-molecules. In the present work, SrMgSi2O6:Eu0.01,Dy0.02 persistent luminescence microspheres have been synthesized via a simple template method and sunlight could successfully excite their luminescence. Further, a new probe was established based on turn-off of the persistent luminescence emission for detection and optical imaging of dopamine (DA). Mechanistic studies indicated that when the synthesized PL microspheres themselves are alkaline, the PL of the SrMgSi2O6:Eu0.01,Dy0.02 microspheres can be efficiently quenched by DA through electron transfer in the presence of ambient O2. The approach exhibited high convenience by only homogeneously mixing DA and the SrMgSi2O6:Eu0.01,Dy0.02 microspheres. Meanwhile, other phenols, ascorbic acid (AA), and uric acid (UA) were tested and had little interference to the dopamine detection. The current strategy showed the linear range of PL intensity versus concentration was 1.0 × 10−5–1.0 × 10−3 M for DA with a detection limit of 0.78 μM (S/N = 3) and it was successfully applied for imaging and detection of DA in human urine with quantitative recovery (99.6–102.5%).
研究领域:
分析化学
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生物医学工程
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DOI
PubMed
高影响因子
DALTON TRANSACTIONS
·
2015
·Vol. 45
·期 6
·2686-2693
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IF 4.197
Pengpeng Lei,Xiuling Liu,Lile Dong,Zhuo Wang,Shuyan Song,Xia Xu,Yue Su,Jing Feng,Hongjie Zhang
Water-soluble lanthanide (Ln3+) doped Bi2O3 nanospheres have been successfully prepared through a solid-state-chemistry thermal decomposition process. The nanospheres exhibit intense upconversion luminescence (UCL) by doping the Ln3+ (Ln = Yb, Er/Ho/Tm) ions into the Bi2O3 host matrix under 980 nm excitation. The ratio of red/green emission of Bi2O3:Yb3+/Er3+ nanospheres exhibits a significant change as the calcination temperature increases and the value could reach 105.6. Moreover, the UCL of Bi2O3:Yb3+/Tm3+ nanospheres are temperature-sensitive, where the intensity ratios of 799 and 808 nm emissions increase monotonously with temperature. The MTT assay reveals that Bi2O3:Yb3+/Tm3+ nanospheres exhibit good biocompatibility by grafting citric acid molecules on the surface. The application possibility of Bi2O3:Yb3+/Tm3+ nanospheres as bioprobes for optical imaging in vivo is also confirmed by the high-contrast photoluminescence images between the background and the UCL imaging area.
研究领域:
材料科学
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生物医学工程
·
光学
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2019
·
IF 5.714
Hui Huang,Chao Wang,Shuchang Liu,Zehao Sun,Dejun Zhang,Caicai Liu,Yang Jiang,Shuyue Zhan,Haofei Zhang,Ren Xu
The automatic identification (location, segmentation , and classification) by UAV- based optical imaging of spills of transparent floating Hazardous and Noxious Substances (HNS) benefits the on-site response to spill incidents, but it is also challenging. With a focus on the on-site optical imaging of HNS, this study explores the potential of single spectral imaging for HNS identification using the Faster R-CNN architecture. Images at 365 nm (narrow UV band), blue channel images (visible broadband of ∼400–600 nm), and RGB images of typical HNS (benzene, xylene, and palm oil) in different scenarios were studied with and without Faster R-CNN. Faster R-CNN was applied to locate and classify the HNS spills. The segmentation using Faster R-CNN-based methods and the original masking methods, including Otsu, Max entropy, and the local fuzzy thresholding method (LFTM), were investigated to explore the optimal wavelength and corresponding image processing method for the optical imaging of HNS. We also compared the classification and segmentation results of this study with our previously published studies on multispectral and whole spectral images. The results demonstrated that single spectral UV imaging at 365 nm combined with Faster R-CNN has great potential for the automatic identification of transparent HNS floating on the surface of the water. RGB images and images using Faster R-CNN in the blue channel are capable of HNS segmentation.
研究领域:
环境科学
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图像处理
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遥感技术
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DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Yaoyao Liu,Jing-Min Liu,Dongdong Zhang,Kun Ge,Peihua Wang,Huilin Liu,Guozhen Fang,Shuo Wang
Probiotics has attracted great attention in food nutrition and safety research field, but thus far there are limited analytical techniques for visualized and real-time monitoring of the probiotics when they are ingested in vivo. Herein, the optical bioimaging technique has been introduced for investigation of foodborne probiotics biodistribution in vivo, employing the near-infrared (NIR) emitting persistent luminescence nanophosphors (PLNPs) of Cr3+-doped zinc gallogermanate (ZGGO) as the contrast nanoprobes. The ultrabrightness, super long afterglow, polydispersed size, low toxicity, and excellent photostability and biocompatibility of PLNPs were demonstrated to be qualified as a tracer for labeling probiotics via antibody (anti-Gram positive bacteria LTA antibody) recognition as well as contrast agent for long-term bioimaging the probiotics. In vivo optical bioimaging assay showed that the LTA antibody functionalized ZGGO nanoprobes that could be efficiently tagged to the probiobics were successfully applied for real-time monitoring and nondamaged probing of the biodistribution of probiotics inside the living body after oral administration. This work presents a proof-of-concept that exploited the bioimaging methodology for real-time and nondamaged researching the foodborne probiotics behaviors in vivo, which would open up a novel way of food safety detection and nutrition investigation.
研究领域:
食品科学
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生物医学工程
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营养学
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DOI
PubMed
高影响因子
Regenerative Biomaterials
·
2021
·Vol. 8
·期 3
·
IF 6.353
Yang Li,Fu Shengxiang,Liu Li,Cai Zhongyuan,Xia Chunchao,Song Bin,Gong Qiyong,Lu Zhiyun,Ai Hua
Magnetic resonance (MR)/optical dual-mode imaging with high sensitivity and high tissue resolution have attracted many attentions in biomedical applications. To avert aggregation-caused quenching of conventional fluorescence chromophores, an aggregation-induced emission molecule tetraphenylethylene (TPE)-conjugated amphiphilic polyethylenimine (PEI) covered superparamagnetic iron oxide (Alkyl-PEI-LAC-TPE/SPIO nanocomposites) was prepared as an MR/optical dual-mode probe. Alkyl-PEI-LAC-TPE/SPIO nanocomposites exhibited good fluorescence property and presented higher T2 relaxivity (352 Fe mM−1s−1) than a commercial contrast agent Feridex (120 Fe mM−1s−1) at 1.5 T. The alkylation degree of Alkyl-PEI-LAC-TPE effects the restriction of intramolecular rotation process of TPE. Reducing alkane chain grafting ratio aggravated the stack of TPE, increasing the fluorescence lifetime of Alkyl-PEI-LAC-TPE/SPIO nanocomposites. Alkyl-PEI-LAC-TPE/SPIO nanocomposites can effectively labelled HeLa cells and resulted in high fluorescence intensity and excellent MR imaging sensitivity. As an MR/optical imaging probe, Alkyl-PEI-LAC-TPE/SPIO nanocomposites may be used in biomedical imaging for certain applications.
研究领域:
生物医学工程
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材料科学
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影像医学
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产品关联
DOI
PubMed
高影响因子
Science China-Chemistry
·
2021
·Vol. 65
·期 2
·339-352
·
IF 9.445
Wang Yipu,Mei Dong,Zhang Xinyi,Qu Da-Hui,Mei Ju
Precise and early detection of β -amyloid (A β ) deposits in situ and in real time is pivotal to the diagnosis and early intervention of Alzheimer’s disease (AD). Optical imaging stands out to be a promising technique for such a task; however, it still remains a big challenge, due to the lack of high-performance imaging contrast agent. Restricted by poor blood-brain barrier (BBB) penetrability, short-wavelength excitation and emission, as well as the aggregation-caused quenching effect, the widely used gold-standard probes cannot be used for early in-vivo imaging of A β deposits. Herein, we integrate the A β deposits-favored geometry, amphiphilic and zwitterionic molecular structure, extended D-π-A electronic structure, and 3D conformation into one molecule, facilely establishing a simple and economic imaging contrast agent that enjoys high specificity and affinity to A β deposits, good BBB penetrability, bright red/near-infrared fluorescence, low interference from autofluorescence, aggregation-induced emission (AIE) feature, high signal-to-noise ratio (SNR), and high contrast. In-vitro, ex-vivo , and in-vivo experiments with different strains of mice indicate that AIE-CNPy-AD holds the universality to A β deposits identification. Noteworthily, AIE-CNPy-AD is even able to precisely trace the small and sparsely-distributed A β deposits in AD model mice as young as 4-month-old APP/PS1 mice, the youngest having A β deposits. Moreover, the present probe could clearly reveal the increase and enlargement of A β deposits as the mice grow. Therefore, AIE-CNPy-AD might be an ideal alternative for early AD diagnosis and highly reliable monitoring of AD progression.
研究领域:
神经生物学
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医学影像学
·
分子探针设计
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DOI
高影响因子
JOURNAL OF NEUROSCIENCE METHODS
·
2022
·
IF 2.987
Yao He,Yingqian Zhang,Wanxin Li,Qinxi Li,Bangcheng Zhao,Xun Tang,Daojun Chen,Ting Zhang,Tao Zhang,Zhihui Zhong
Background Blood-brain barrier (BBB) disruption is pivotal in the pathophysiological process of ischemic stroke and is often measured in rodent stroke studies. Traditionally, rodent BBB permeability increase is determined by measuring cerebral leakage of certain dyes such as Evans Blue or sodium fluorescein (NaFL). However, due to the special processing of samples for BBB permeability measurement, they cannot be used afterward for determining other essential parameters such as cerebral infarction volume. Therefore, using different batches of animals for assessing BBB permeability and infarction volume is typical. However, this would limit the stroke study’s statistical power and scientific value while hindering the implementation of procedures for high standard animal welfare. New method The rats subjected to middle cerebral artery occlusion (MCAO) were intraperitoneally injected with NaFL during the reperfusion phase. The brains were sliced and measured for BBB permeability using the small animal optical imaging system (IVIS® Lumia series III). Afterward, the same brain samples were either sliced or homogenized for tests that assessed infarction volume or other molecular changes. Results The sum fluorescence intensity of the ischemic brain slices under the IVIS® Lumia series Ⅲ showed a strong correlation with the infarction volume determined by 2,3,5-triphenyl tetrazolium chloride (TTC) staining (r = 0.7440, P = 0.0087). The fluorescence intensity of the whole ischemic brain was correlated with the NaFL concentration of brain tissue homogenates (r = 0.8653, P = 0.0026) and cerebral infarction volume (r = 0.7282, P = 0.0072). Comparison with existing methods The new method enables concurrent measurement of BBB permeability and infarction volume on the same batch of brain tissue samples without affecting most downstream biochemical assays. Conclusions By applying the new method, we could use the same batch of ischemic rodent brain tissue for multiple assays, including BBB permeability and infarction volume. Through this, we would reduce the animal numbers in each study and help to maximize the scientific and statistical potential of future rodent ischemic studies.
研究领域:
神经科学
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生物医学工程
·
药理学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2015
·Vol. 4
·期 6
·1100-1107
·
IF 4.726
Ruiqing Liu,Shuang Liang,Cun Jiang,Li Zhang,Tianmeng Yuan,Penghui Li,Zushun Xu,Haibo Xu,Paul K. Chu
Smart polymeric particles possessing the advantages of paramagnetism, luminescence, and controlled drug delivery in a single entity are reported. Gadolinium oxide and europium-encapsulated temperature/pH-responsive polymeric particles (PLTPPs) synthesized by emulsifier-free emulsion polymerization show good biocompatibility with C6 cells and anticancer drug (doxorubicin, DOX) loading capability. In vitro drug release assessment discloses release abatement under acidic conditions or at high temperature, and the DOX-loaded PLTPPs have obvious antitumor properties for C6 and H22 cells. Cellular uptake tests confirm that the materials can be taken up by C6 cells thereby facilitating optical imaging. The T1-weighted relaxivity value at 3 T is 6.13 mM−1 s−1 which is 39% higher than that of the clinical Magnevist®. In vivo MR and optical imaging reveal that the PLTPPs are effective dual probes. The results indicate that the PLTPPs have great potential in tumor diagnosis and treatment.
研究领域:
医学影像学
·
生物材料学
·
肿瘤学
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产品关联
DOI
PubMed
高影响因子
Biomaterials Science
·
2020
·Vol. 9
·期 3
·1000-1007
·
IF 6.183
Bi Lin,Jun Wu,Yanxing Wang,Song Sun,Ying Yuan,Xiaofeng Tao,Ruichan Lv
Early diagnosis is critical and challenging for tongue squamous cell carcinoma (TSCC), which is a kind of tumor with high malignancy, poor prognosis, and a high incidence of invasion and metastasis. In this research, dual-modal optical imaging rare earth nanoparticle (RENP) probes with peptide functionalization are designed for targeted TSCC imaging and therapy. RENP@C@Au (UCA) with enhanced red upconversion luminescence (UCL) and near infrared II (NIR II) imaging intensity is designed by metal and codopant modulation, and the two peaks at about 650 nm and 1064 nm of Nd ions are relatively stable with less quenching and suitable for luminescence bioimaging. Then, the cMBP peptide targeted to Cal 27 TSCC cells with highly expressed c-MET proteins is combined with UCA. Compared with human's normal cells of MCF-10A, other tumor cell lines such as A549 and HeLa, together with mice tumor cells of 4T1, the designed probe has targeted imaging and therapy to Cal 27. The final in vivo experiment shows that the probe with high NIR II luminescence signals is not easy to retain when injected into the tail vein, indicating its potential precise clinical application for the diagnosis and therapy of TSCC.
研究领域:
生物医学工程
·
肿瘤学
·
纳米医学
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）