ANALYTICA CHIMICA ACTA
·
2024
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IF 5.7
Jingman Zhang,Yiying Luo,Yiyu Chen,Huiting Lian,Bin Liu,Chunnuan Chen,Xiaofeng Wei
Background Temperature sensing is commonly used in point-of-care (POC) detection technologies, yet the portability and convenience of use are frequently compromised by the complexity of thermosensitive processes and signal transduction. Especially, multi-step target recognition reactions and temperature measurement in the reaction vessel present challenges in terms of stability and integration of detection devices. To further combine photothermal reaction and signal readout in one assay, these two processes enable to be integrated into miniaturized microfluidic chips, thereby facilitating photothermal sensing and achieving a simple visual temperature sensing as POC detection. Results A copper ion (Cu 2+ )-catalyzed photothermal sensing system integrated onto a microfluidic distance-based analytical device (μDAD), enabling the visual, portable, and sensitive quantitative detection of multiple targets, including ascorbic acid, glutathione, and alkaline phosphatase (ALP). The polydopamine nanoparticles (PDA NPs) were synthesized by the regulation of free Cu 2+ through redox or coordination reactions, facilitating the transduction of distinct photothermal response signals and providing the versatile Cu 2+ -responsive sensing systems. Promoted by integration with a photothermal μDAD, the system combines PDA's photothermal responsiveness and thermosensitive gas production of ammonium bicarbonate for improved sensitivity of ALP detection, reaching the detection limit of 9.1 mU/L. The system has successfully achieved on-chip detection of ALP with superior anti-interference capability and recoveries ranging from 96.8 % to 104.7 %, alongside relative standard deviations below 8.0 %. Significance and novelty The μDAD design accommodated both the photothermal reaction of PDA NPs and thermosensitive gas production reaction, achieving the rapid sensing of visual distance signals. The μDAD-based Cu 2+ -catalyzed photothermal sensing system holds substantial potential for applications in biochemical analysis and clinical diagnostics, underscored by the versatile Cu 2+ regulation mechanism for a broad spectrum of biomarkers.
研究领域:
生物化学
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医学诊断
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分析化学
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产品关联
DOI
PubMed
高影响因子
APPLIED PHYSICS LETTERS
·
2022
·Vol. 121
·期 5
·
IF 3.971
Zhang Wenjuan,Miao Yinping,Zhang Hongmin,Yao Jianquan
Streptomycin sulfate (STS) is a broad-spectrum antibiotic widely present in water as a source of pollution. STS produces ototoxicity and nephrotoxicity if ingested in excess. Here, we propose a photothermal sensor to detect contaminated water based on a Nb 2 CTx MXene integrated few-mode fiber coupler (FMFC) and the photothermal effect of STS. Nb 2 CTx MXene exhibits strong absorption ability for biomolecules, which can enhance the interaction between STS and light by depositing it on the surface of the FMFC, thereby improving the detection performance of the proposed sensor. Under irradiation of a 405 nm laser, the photon energy is absorbed by STS and converted into heat energy, changing the transmission characteristics of the few-mode fiber. The results show that the sensitivity reaches 210.66 nm/(mg/ml) in the linear range of 0.02–0.1 mg/ml, with a detection limit of 94.93 ng/ml. In practical applications, the photothermal effect is stimulated by natural light to improve the suitability of the device application. Furthermore, this photothermal sensor eliminates complicated sample processing, making it potentially applicable for real-time food safety, environmental pollutant detection, and disease diagnosis.
研究领域:
环境科学
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生物医学工程
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光学工程
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DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
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2025
·
IF 6.6
Shanglin Li,Ziyang He,Yonghong Meng,Baoxin Li,Mei Liu
Aflatoxin B1 contamination poses severe threats to food safety, vitally requiring reliable assays. Biosensors are effective but subject to unsatisfactory signal conversion efficiency owing to their usage of the monovalent probes with redundant materials and procedures. Herein, we developed a photothermal aptasensor, which combined multifunctional polyvalent DNA scaffolds with thermal imaging for the portable detection of aflatoxin B1. The aptasensor was conferred with favorable properties of specific recognition, surface modification and signal amplification by the polyvalent DNA scaffolds, thereby showed enhanced analytical performance with simplified procedures. Moreover, the detection potential of the aptasensor was further improved by introducing Cu x S nanoparticles to provide intuitive temperature output for portable devices analysis. Consequently, AFB1 could be quantified in a wide range of 1–500 ng/mL with the detection limit of 0.75 ng/mL, merely using common thermometer. For food samples, the spiked recoveries of the proposed method ranged from 93.5 % to 108.7 % with relative standard deviation from 2.9 % to 6.3 %. These results were in agreement with the high-performance liquid chromatography method, demonstrating the good accuracy of this method. Therefore, this method is expected to be extended as a universal detection strategy for food hazards analysis.
研究领域:
食品科学
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生物传感技术
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分析化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
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2024
·
IF 8.5
Zhen Zhang,Zhenkai Hao,Ruohan Yang,Ruiping Shan,Xiangyang Li,Hongyan Zhang
Given the serious harm caused by dietary intake of diethylstilbestrol (DES), it is urgent to explore rapid and sensitive DES sensing methods. In this work, a photothermal DES immunochromatography sensor based on covalent organic framework (COF) was constructed. The performance of COF in the field of photothermal sensing was systematically investigated for the first time. A donor–acceptor type of COF with a photothermal conversion rate of 51.17 % was synthesized. The logarithm of the DES concentrations-temperature change value standard curve was plotted. The intensity of the photothermal sensing signal was inversely proportional to the sample concentration. The detection limit of the proposed photothermal method (0.24 μg·L −1 ) was 10 times higher than that of visual detection (3 μg·L −1 ). This work not only constructed a novel detection method for DES sensing, but also provided a feasible demonstration for the application of COF in photothermal sensing and expanded the application of their photothermal properties.
研究领域:
分析化学
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材料科学
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传感器与执行器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
·
IF 4.6
Haowei Huang,Tai Wen,Junyao Li,Laidi Xu,Wenrong Cai,Yong Kong
As the chiral source, L-histidine (L-His) is used for the decoration of copper sulfide nanoparticles (CuS NPs), which is further utilized to fabricate a colorimetric and photothermal dual-mode chiral sensor for the optical resolution of aspartic acid (Asp) isomers. Since the optical activity of L-His is consistent with L-Asp, the CuS-L-His shows higher affinity for L-Asp than D-Asp, and thus more 3,3′,5,5′-tetramethylbenzidine (TMB) is captured by CuS-L-His/L-Asp through electrostatic attractions compared with CuS-L-His/D-Asp. Owing to the superior peroxidase-like activity of CuS NPs, the captured TMB can be catalyzed to blue oxidized TMB (oxTMB) in the presence of H 2 O 2 , and thus the optical resolution of the Asp isomers can be realized by the colorimetric mode. In addition, the nanosized CuS has outstanding photothermal conversion ability, and the combination with Asp isomers may lead to the decrease in temperature. Since the decrease in the temperature of CuS-L-His/L-Asp is more pronounced than that of CuS-L-His/D-Asp, the Asp isomers can also be optically resolved by the photothermal mode. The proposed dual-mode chiral sensor is more attractive than conventional single-mode chiral sensors, since it can provide higher accuracy for the optical resolution.
研究领域:
分析化学
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生物化学
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Miao Ma,Qing Wang,Qianqian Su,Weizhe Liu,Hui Xia,Caixia Zhou,Mengtian Li,Fang Yang,Fujian Xu
Blood uric acid (UA) levels are closely associated with various diseases. This study presents a novel UA photothermal detection system. UA reacts with uricase to generate hydrogen peroxide, which then oxidizes 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidized TMB (oxTMB) in the presence of copper ions as the catalyst. Crucially, instead of expensive laser, Light Emitting Diode (LED) was applied to offer the irradiation on oxTMB, inducing a temperature increase. The increment of temperature measured by a handheld household thermometer exhibits a linear correlation with the concentration of UA in the samples. This method demonstrates enhanced detection sensitivity and excellent anti-interference capabilities. It has been successfully applied to precisely determine UA levels in volunteer blood samples. The sensing platform achieves portable UA detection in human blood within 3 min, featuring a linear response range of 50–400 μM, a detection limit (LOD) of 14.4 μM. This work establishes a novel miniaturized LED-based photothermal (LED-PT) uric acid detection device, holding significant potential for rapid, portable UA monitoring in healthcare and clinical diagnostics.
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产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2025
·
IF 7.7
Xin Lei Xu,Yi Wei Zhou,Yue Yu,Shi Gang Liu,Lei Han
It is imperative to design a highly selective, sensitive, and portable approach for the reliable determination and monitoring of mercury ion (Hg 2+ ) given its severe toxicity to both ecosystems and public health. Herein, we develop a colorimetric and photothermal-derived multimodal sensor for point-of-care testing of Hg 2+ based on the Hg 2+ -sensitized oxidase-mimetic activity of CoSe 2 nanorods. The synthesized CoSe 2 nanorods show negligible oxidase-mimetic activity. It can bind with CoSe 2 nanorods via an Hg-Se bond when Hg 2+ is present. The generation of HgSe on the surface of CoSe 2 nanorods booms the oxidase-like activity of CoSe 2 nanorods to catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) into oxidative TMB (oxTMB), generating a hyperchromic effect and light-driven thermal effect. Consequently, the color and absorbance of the solution changed, realizing the colorimetric and visual sensing of Hg 2+ . The limit of detection (LOD) is 7.3 nM for colorimetry. Meanwhile, a photothermal sensor was also developed under the drive of a laser with a LOD of 12.3 nM. The developed sensor also shows high selectivity toward Hg 2+ because of the strong affinity between the CoSe 2 and Hg 2+ . Furthermore, the latent application of this sensor is confirmed by determining Hg 2+ in real water samples. More significantly, multimodal detection not only enhances accurate detection results through mutual authentication of different signals but also satisfies the demands of diversified detection by consolidating the advantages of photothermal and colorimetric approaches.
研究领域:
分析化学
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环境监测
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纳米生物技术
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传感技术
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重金属检测
✓ 已验证
产品关联
DOI
高影响因子
ACS Food Science & Technology
·
2024
·
IF 2.6
Xin Liu,Guojian Wu,Chao Nie,Fengjiao He,Xiang Gao,Xin Liu,Yongning Wu,Yizhong Shen
Considering the potential misuse of antibiotics to improve the yield and economic benefits in the production of animal-derived foods such as milk, the development of a simple, label-free, precise, reliable, low-cost, and onsite assay for antibiotic residues is highly desirable to ensure food safety. In this scenario, we here proposed a hybrid oxidase-mimicking nanozyme of the Ru@g-C3N4 nanosheet that combines with 3,3′,5,5′-tetramethylbenzidine (TMB) to develop a blocking oxidase-mimicking activity-driven photothermal and colorimetric synergistic sensing platform for intelligent onsite detection of tetracycline antibiotics (TCs). In the absence of TCs, the prominent oxidase-mimicking activity of Ru@g-C3N4 rapidly catalyzed colorless TMB to dark-blue oxTMB, accompanied by the appearance of an obvious absorbance enhancement at 655 nm and a remarkable photothermal signal upon 660 nm laser irradiation. Conversely, the remaining TCs effectively blocked this chromogenic reaction because of the π–π stacking of its tetraphenyl skeleton with the Ru@g-C3N4 nanozyme to block the affinity of TMB to the catalytic active site inside Ru@g-C3N4. Based on this, a TCs residual level-dependent photothermal and colorimetric signal synergistic decrease sensing platform was developed with the assistance of a 660 nm laser-assisted hand-held thermal imager and a Color Picker APP-inbuilt smartphone, which demonstrated the intelligent onsite label-free, reliable, and precise detection of TCs in real milk samples with acceptable results. Notably, this oxidase-mimicking nanozyme-based dual-mode sensing platform provided a built-in cross-reference correction to improve not only the accuracy but also the reliability for TC detection in milk, which showed a vast promise in the further development of commercial point-of-care testing to achieve early warning of antibiotic residues to ensure the quality and safety of animal-derived foodstuffs.
研究领域:
分析化学
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食品科学
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纳米技术
·
生物技术
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Zongyou Chen,Jiaqi Yin,Shijin Huang,Keyang Lai,Juan Peng,Weihua Lai
The photothermal lateral flow immunoassay (LFIA) has garnered considerable attention, owing to its suitability for on-site quantitative detection. Furthermore, it has distinct advantages in further constructing sensitive detection methods for low-concentration targets. In this study, we employed a ligand engineering strategy to synthesize Fe3+-chelated quinone nanoparticles (FQNPs). Quinones with different structures, namely, naphthazarin, quinizarine, purpurin, and tetrahydroxyanthraquinone (THAQ), served as ligands to fabricate a series of FQNPs. Among them, FQNPs based on tetrahydroxyanthraquinone (FQNPs-T) exhibited exceptional light absorption ability (molar extinction coefficient = 12.71 × 1010 M–1 cm–1) and photothermal conversion efficiency (η = 60.32%). Subsequently, a photothermal LFIA based on FQNPs-T (FQNPs-LFIA) was developed for the detection of chlorantraniliprole (CHL) in apple and chili. The FQNPs-LFIA enables the highly sensitive detection of CHL within 25 min with a limit of detection (LOD) of 0.021 ng mL–1, which was 8.77-fold lower than that of conventional gold nanoparticle-based LFIA (0.193 ng mL–1). The average recovery rates of FQNPs-LFIA were 84.29–113.58%, with coefficients of variation of 4.20–14.84%. Overall, this study demonstrates the potential of FQNPs-LFIA for the sensitive and accurate detection of CHL, and it paves the way for the rapid screening of other food contaminants.
研究领域:
分析化学
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纳米技术
·
生物检测
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食品安全
·
环境监测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Ting Ting Li,Hui Xi Li,Qiang Yu,Wen Qing Jia,Shu Ting Li,Wei Zhu,Zhen Chen,Lei Han
It is vital to exploit sensitive and portable means for the reliable assay of acid phosphatase (ACP) activity and its inhibitors screening in clinical diagnosis and drug therapy. Herein, the Pt-Ni alloy nanoparticles (Pt-Ni ANPs) with enhanced oxidase-mimetic activity were successfully prepared and applied to oxidize the achromatous 3,3′,5,5′-tetramethylbenzidine (TMB) to blue oxTMB. The oxTMB, as an effective photothermal agent, can transform photon energy to heat under an 808 nm laser illumination. The absorbance and temperature of the reaction solution increased significantly when TMB was oxidized to oxTMB by the oxidase-like Pt-Ni ANPs. However, the ACP can hydrolyze 2-Phospho-L-ascorbic acid trisodium salt (AAP) to produce ascorbic acid (AA), and then the production of AA, as a powerful reductant, can efficiently reduce oxTMB and react with singlet oxygen ( 1 O 2 ) to suppress the oxidase-mimicking activity of Pt-Ni ANPs, causing an obvious decrease in absorption spectrum and temperature of the assay system. A colorimetric and photothermal dual-mode detection platform was established for ACP activity assay by integrating colorimetric and temperature signals. Moreover, the potential application of this strategy was also verified by sensing ACP activity in human serum samples and its inhibitor screening. This dual-mode sensing platform not only improves sensitivity and precision but also meets the needs of diversity detection, which offers new insights for efficient monitoring of ACP activity and inhibitor screening in resource-poor settings.
研究领域:
分析化学
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生物传感器
·
医学诊断
·
纳米技术
·
临床化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）