SENSORS
·
2016
·Vol. 16
·期 1
·135
·
IF 2.033
Jiexia Chen,Yifan Liu,Guang-Chao Zhao
A novel photoelectrochemical biosensor for step-by-step assay of tyrosinase and thrombin was fabricated based on the specific interactions between the designed peptide and the target enzymes. A peptide chain with a special sequence which contains a positively charged lysine-labeled terminal, tyrosine at the other end and a cleavage site recognized by thrombin between them was designed. The designed peptide can be fixed on surface of the CdTe quantum dots (QDs)-modified indium-tin oxide (ITO) electrode through electrostatic attraction to construct the photoelectrochemical biosensor. The tyrosinase target can catalyze the oxidization of tyrosine by oxygen into ortho-benzoquinone residues, which results in a decrease in the sensor photocurrent. Subsequently, the cleavage site could be recognized and cut off by another thrombin target, restoring the sensor photocurrent. The decrease or increase of photocurrent in the sensor enables us to assay tyrosinase and thrombin. Thus, the detection of tyrosinase and thrombin can be achieved in the linear range from 2.6 to 32 μg/mL and from 4.5 to 100 μg/mL with detection limits of 1.5 μg/mL and 1.9 μg/mL, respectively. Most importantly, this strategy shall allow us to detect different classes of enzymes simultaneously by designing various enzyme-specific peptide substrates.Keywords:tyrosinase;thrombin;peptide;photoelectrochemical biosensor
研究领域:
生物化学
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医学检测
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纳米技术
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产品关联
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2025
·Vol. 192
·期 9
·1-9
·
IF 5.3
Si Zhongzhou,Shao Rong,Li Ting,Yang Minghui
Photoelectrochemical biosensor based on Bi 2 S 3 /Bi 2 O 2 CO 3 heterojunction as photoelectrically active material and lactate oxidase was constructed for sensitive detection of lactic acid in serum. Topological transformation method was utilized to prepare Bi 2 S 3 /Bi 2 O 2 CO 3 composites, which can generate electron–hole pairs triggering photoelectrochemical reactions under light illumination. The Bi 2 S 3 /Bi 2 O 2 CO 3 heterojunction effectively promoted the separation and transportation of photogenerated electrons and holes, leading to improved photoelectric conversion efficiency. Lactic acid is catalyzed by lactate oxidase to generate H 2 O 2 , which reacts with photogenerated holes, leading to photocurrent increase, achieving quantitative detection of lactic acid. The prepared biosensor has a wide detection range for lactic acid from 0.01 mM to 10 mM, with detection limit as low as 0.006 mM. In addition, the detection of lactic acid in serum samples further validated the potential application of the sensor. Graphical abstract
研究领域:
生物传感器
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临床检验
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纳米材料应用
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光电化学分析
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生物医学检测
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Mei Xiang,Chengyan Yin,Qiulian Chen,Guonian Zhu,Zhaoxia Chen,Hailiang Li,Ruth Antwi-Baah,Jiangwen Tang,Kunhong Yang,Shuxia Xu
A DNAzyme-based photocatalytic electrochemical biosensor was developed for the label-free detection of uranyl ions (UO 2 2+ ) in water. In this sensing platform, SYBR Green (SG) molecules were embedded in double-stranded DNA (dsDNA), which was cleaved via formed singlet oxygen ( 1 O 2 ) during photosensitization, resulting in a significant decrease in the impedance signal of [Fe (CN) 6 ] 3−/4− . In the presence of UO 2 2+ , the substrate strand was cleaved by the target UO 2 2+ , releasing the SG molecule from the dsDNA, and the photosensitive reaction was turned off. Therefore, UO 2 2+ can be detected based on changes in [Fe(CN) 6 ] 3−/4− electrochemical signals. Such a sensing strategy allowed the detection of UO 2 2+ in the concentration range of 1–1000 nM, with a limit of detection (LOD) of 1.3 × 10 −10 M. The LOD meets the maximum allowable contamination level (1.3 × 10 −7 M) of UO 2 2+ in drinking water set by the US Environmental Protection Agency (EPA). The proposed sensor has been successfully applied for detection of UO 2 2+ in spiked actual water samples with recoveries in the range of 88.0 %–103 %, comparable to those obtained by ICP-MS. The method, which combines photocatalysis with an electrochemical biosensor, significantly improved the sensitivity for UO 2 2+ detection without the need of labeling and additional signal amplification steps. It is also a promising strategy for rapid monitoring of UO 2 2+ in environmental water samples.
研究领域:
环境科学
·
分析化学
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生物传感技术
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产品关联
DOI
高影响因子
SENSORS
·
2024
·Vol. 24
·期 3
·773
·
IF 3.4
Hong Liu,Weiwei Wei,Jiajun Song,Jin Hu,Zhezhe Wang,Peng Lin
In this work, we report a new concept of upconversion-powered photoelectrochemical (PEC) bioanalysis. The proof-of-concept involves a PEC bionanosystem comprising a NaYF4:Yb,Tm@NaYF4upconversion nanoparticles (UCNPs) reporter, which is confined by DNA hybridization on a CdS quantum dots (QDs)/indium tin oxide (ITO) photoelectrode. The CdS QD-modified ITO electrode was powered by upconversion absorption together with energy transfer effect through UCNPs for a stable photocurrent generation. By measuring the photocurrent change, the target DNA could be detected in a specific and sensitive way with a wide linear range from 10 pM to 1 μM and a low detection limit of 0.1 pM. This work exploited the use of UCNPs as signal reporters and realized upconversion-powered PEC bioanalysis. Given the diversity of UCNPs, we believe it will offer a new perspective for the development of advanced upconversion-powered PEC bioanalysis.Keywords:photoelectrochemical biosensors;upconversion nanoparticles;energy transfer;photo reabsorption
研究领域:
生物分析学
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纳米技术
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF THE CHINESE CHEMICAL SOCIETY
·
2021
·Vol. 68
·期 11
·2194-2201
·
IF 1.967
Yanyan Niu,Yuxue Chen,Xiaoping Zhang,Hui Xie,Guiling Luo,Wei Sun
Lead ion causes heavy metal pollution, which can cause serious damage to the ecological environment and human health. In this paper, a simple and sensitive photoelectrochemical method was designed for lead ion detection by using ZnO nanorods as the photoactive substrate and complementary strand aptamers as target recognition element. ZnO nanorods with favorable stability and photoelectric activity can provide a biocompatible microenvironment for aptamer loading. In the presence of lead ion, it would combine with the recognition aptamer S2 by competing with the aptamer S1, which led to changes in surface structure and charge density before and after the recognition of target, causing regular response of photocurrent. The developed method under optimized experimental conditions presented high sensitivity and good selectivity for lead ion detection with low detection limit as 3.5 × 10 −11 mol/L. In addition, the proposed strategy was also further applied to the detection of lake water sample, which provided reliable experimental support and basis for further exploration and establishment of complex aptamer recognition and detection system.
研究领域:
环境科学
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分析化学
·
生物传感技术
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DOI
高影响因子
MICROCHIMICA ACTA
·
2017
·Vol. 184
·期 8
·2541-2549
·
IF 4.58
Han Zhizhong,Luo Min,Chen Li,Pan Haibo,Chen Jinghua,Li Chunyan
The work describes a photoelectrochemical (PEC) biosensor for the detection of DNA. It employs oriented hierarchical ZnO flower-rod architectures (ZnO FRs) and DNA dendrimers. ZnO FRs act as photoactive material that yields a photocurrent of up to 23 μA. The photogenerated electron transfer is inhibited once the probe DNA and the target DNA oligonucleotides hybridize, and this results in a reduced photocurrent. The use of DNA dendrimers with scores of DNA branches further amplifies the signal of the PEC biosensor. The PEC sensor displays a response that is linear in the DNA concentration range from 10 fM to 0.1 μM with a detection limit of 3.7 fM (at S/ N = 3). The sensor was applied to the determination of DNA in human serum samples and was found to work with acceptable accuracy. Due to the use of ZnO FRs and DNA dendrimers, the assay is highly sensitive, rapid, and repeatability.
Schematic of an innovative photoelectrochemical (PEC) biosensors based on oriented ZnO flower-rod architectures (ZnO FRs) for DNA detection. Dendrimers were employed for signal amplification to give a low detection limit of 3.7 fM.
研究领域:
分析化学
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生物传感器
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材料科学
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纳米技术
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DOI
高影响因子
MICROCHIMICA ACTA
·
2021
·Vol. 189
·期 1
·1-10
·
IF 5.833
Meng Yuchan,Qin Nana,Hun Xu
ZnSe nanodisks:Ti 3 C 2 MXene complex was prepared for the first time. Based on its remarkable photoelectrochemical performance, combined with the enzyme-free toehold-mediated strand displacement reaction, a photoelectrochemical biosensor for the detection of the non-small-cell cancer biomarker ctDNA KRAS G12D was developed. ZnSe nanodisks were in situ grown on Ti 3 C 2 MXene surface by two-step hydrothermal method. The high conductivity and adjustable band gap of MXene significantly enhanced the photoelectric response of ZnSe. Subsequently, the photoelectrochemical biosensor was prepared by combining with the signal amplification function of p- aminophenol and the enzyme-free toehold-mediated strand displacement reaction on the modified ITO electrode surface. Under the optimized conditions, the linear detection range is 0.5 ~ 100.0 fM, and the detection limit is 0.2 fM, which realizes the sensitive detection of KRAS G12D. The photoelectrochemical biosensor constructed opens up a new pathway for the preparation of new Mxene-based composite materials and the research of photoelectrochemical biosensor. Graphical abstract Nucleic acid liquid biopsy with ZnSe nanodisks:Ti 3 C 2 MXene photoelectroactive modified electrode
研究领域:
材料科学
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生物医学工程
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分析化学
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癌症诊断
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Ruhao Wang,Mingjing Wang,Xu Hun
Coxsackievirus B3 (CVB3), a member of the genus Enterovirus, has high risks for incidence and mortality. Developing speedy and sensitive molecular diagnostics for CVB3 detection is challenging. Herein, a novel highly sensitive photoelectrochemical (PEC) biosensor based on nanocomposite (ZnSeNSs/AuNPs/BNNSs) modified electrode was fabricated. This biosensor relied on reverse transcription of CVB3 RNA and the rapid isothermal amplication by recombinase polymerase amplification (RPA). The amplified product was identified and linked to photoelectrode. Then biotin on the amplified product was connected to strepavidin-alkaline phosphatase (SA-ALP). The alkaline phosphatase catalyzed the hydrolysis of substrate 4-aminophenylphosphate monosodium (4-APP) to generate free 4-aminophenol which was a electron donor. An enhancing PEC signal was obtained. Therefore, a relationship between photocurrent response and CVB3 concentration was established. Under the optimal conditions, this PEC biosensor displayed a liner photocurrent response in the range of 0.1 fM ∼100 fM with a low detection limit of 30 aM. Furthermore, this strategy with satisfactory stability and selectivity provides an innovative way for CVB3 detection and lays a foundation for the research of portable devices for clinical sample processing and analysis in the further.
研究领域:
病毒学
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生物化学
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生物医学工程
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分析化学
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DOI
高影响因子
CHINESE JOURNAL OF CHEMISTRY
·
2021
·Vol. 40
·期 2
·247-255
·
IF 6
Qian Wang,Huanshun Yin,Yunlei Zhou,Lulu Cao,Zhengkun Yu,Yamin Xu,Shiyun Ai
Comprehensive Summary As a key epigenetic modification, 5-formylcytosine (5fC) controls many important cell functions, such as gene expression and cell differentiation. However, 5fC exists in mammalian tissues and cells with low content, and its structure is highly similar to 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC) and 5-carboxycytosine (5caC), which requires the detection method for detecting 5fC with high sensitivity and specificity. In this experiment, WS 2 /Bi/Bi 2 O 2 CO 3 was used as the photoactive material, 5fC antibody was used as the target molecule recognition reagent, and rolling amplification (RCA) was employed as the signal amplification strategy to construct a novel photoelectrochemical biosensor for detecting 5-formylcytosine deoxynucleotide (5fdCTP). Firstly, WS 2 /Bi/Bi 2 O 2 CO 3 nanocomposite was prepared and characterized, and then modified on the ITO electrode. Then, polyacrylic acid (PAA) was immobilized by electrostatic adsorption, which was employed as the antibody capture reagent based on the covalent reaction of –COOH on PAA with –NH 2 on Ab. Subsequently, 5fdCTP was recognized through immunoreaction, triggering the subsequent rolling circle amplification reaction with the aid of the crosslinker of Zr 4+ . Finally, positive methylene blue (MB) was adsorbed by negative ssDNA, resulting in an increased photoelectric conversion efficiency of WS 2 /Bi/Bi 2 O 2 CO 3 and improved detection sensitivity. The linear range of the PEC biosensor for detecting 5fdCTP was 0.05—100 nmol/L, and the minimum detection concentration was 12.4 pmol/L (S/N = 3). The applicability of this method has been evaluated by studying the influence of Cd 2+ and antibiotic concentration on the 5fC content in the genomic DNA of maize seedlings.
研究领域:
生物化学
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分子生物学
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分析化学
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DOI
高影响因子
SENSORS
·
2018
·Vol. 18
·期 9
·2980
·
IF 2.475
Zhizhong Han,Qinghua Weng,Chaofan Lin,Jinquan Yi,Jie Kang
In this work, the CdSe–ZnO flower-rod core-shell structure (CSZFRs) was prepared by ion-exchange method. The surface of CSZFRs was modified by 3-mercaptopropionic acid (MPA), and then the DNA probe was immobilized on the surface via chemical bond between -NH2of DNA probe and -COOH of MPA. Finally, the target norovirous (NV) RNA was combined with the probe according to the principle of complementary base pairing, resulting in a decrease of the photocurrent. The results show that the absorbance spectrum of visible light is enhanced for CSZFRs compared with pure ZnO. Under visible light irradiation, the photocurrent of CSZFRs is up to 0.1 mA, which can improve the sensitivity of the photoelectrochemical (PEC) biosensor. In the measurement range of 0–5.10 nM, the measured concentrations (c) have a good linear relationship with the output photocurrent of the biosensor. The linear regression equation is expressed asI= 0.03256 − 0.0033c(R2= 0.99, S/N = 3) with a detection limit of 0.50 nM. Therefore, this work realizes a rapid and sensitive method for the detection of NV RNA.Keywords:CdSe–ZnO flower-rod;photoelectrochemical biosensor;norovirous RNA;ion-exchange method
研究领域:
材料科学
·
生物化学
·
分析化学
·
病毒学
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产品关联
DOI
PubMed
高影响因子
Science China-Chemistry
·
2020
·Vol. 63
·期 7
·1012-1018
·
IF 6.356
Guo Xiaoxi,Wu Jinghua,Xia Lian,Xiang Meihao,Qu Fengli,Li Jinghong
Photoelectrochemical (PEC) biosensors have shown great promise in bioanalysis and diagnostic applications in recent years. In this work, the CuO/Cu 2 O nanowire array (CuO/Cu 2 O Nanowire) supported on copper foam was prepared as a photocathode for detection of tyrosinase though quinone-chitosan conjugation chemistry method. The in-situ generated quinones that were the catalytic product of tyrosinase acted as electron acceptors, which were captured by the chitosan deposited on the surface of the electrode. Direct immobilization of electron acceptor on the electrode surface improved the photocurrent conversion efficiency and thus sensitivity. The as-prepared biosensor can realize a rapid response in a wide linear range of 0.05 U/mL to 10 U/mL with the detection limit as low as 0.016 U/mL of tyrosinase. The current work provides a new perspective to design and develop highly sensitive and selective PEC biosensor.
研究领域:
生物分析
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诊断技术
·
光电化学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 8
·1-9
·
IF 6.232
Tang Juan,Li Jingjing,Xiong Pengyuan,Sun Yuanfang,Zeng Zhiyao,Tian Xiaochun,Tang Dianping
A photoelectrochemical (PEC) aptasensing platform is devised for sensitive detection of an organophosphorus pesticide based on dissolution of core-shell MnO 2 nanoflower@CdS (MnO 2 NF@CdS) by thiocholine (TCh). TCH is produced from the butyrylcholinesterase-acetylthiocholine system, accompanied by target-triggered rolling circle amplification (RCA). The core-shell MnO 2 NF@CdS with excellent PEC performance was synthesized and employed as a photo-sensing platform. The target was detected on a functionalized magnetic probe with the corresponding aptamer. Upon malathion introduction, the aptamer was detached from the magnetic beads, while capture DNA (cDNA, with primer fragment) remained on the beads. The primer fragment in cDNA can trigger the RCA reaction to form a long single-stranded DNA (ssDNA). Furthermore, a large number of butyrylcholinesterase (BChE) were assembled on the long ssDNA strands through the hybridization with the S 2 -Au-BChE probe. Thereafter, TCh generated from hydrolysis of ATCh by BChE can reduce MnO 2 NF (core) to Mn 2+ and release the CdS nanoparticles (shell) from the platform electrode, significantly enhancing the PEC signal. Under optimal conditions, the proposed aptasensor exhibited high sensitivity for malathion with a low detection limit of 0.68 pg mL −1 . Meanwhile, it also presents outstanding specificity, reproducibility, and stability. Importantly, the sensing platform provides a new concept for detection of pesticide.
Herein, this work devised a photoelectrochemical (PEC) aptasensing platform for sensitive detection of organophosphorus pesticide based on dissolution of core-shell MnO 2 nanoflower@CdS (MnO 2 NF@CdS) by the as-produced thiocholine (TCh) from the butyrylcholinesterase-acetylthiocholine system, accompanying with the target-triggered rolling circle amplification (RCA).
研究领域:
分析化学
·
环境科学
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生物化学
·
农业科学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）