ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2014
·
IF 2.482
Xinghao Wang,Ruijuan Qu,Zhongbo Wei,Xi Yang,Zunyao Wang
Mercury (Hg) compounds are widely distributed toxic environmental and industrial pollutants and they may bring danger to growth and development of aquatic organisms. The distribution of Hg species in the 3 percent NaCl solution was calculated using the chemical equilibrium model Visual MINTEQ, which demonstrated that Hg was mainly complexed by chlorides in the pH range 5.0–9.0 and the proportions of HgCl 4 2− , HgCl 3 − and HgCl 2 (aq) reached to 95 percent of total Hg. Then the effects of cations (Ca 2+ , Mg 2+ , K + and H + ), anions (HCO 3 − , NO 3 − , SO 4 2− and HPO 4 2− ) and complexing agents (ethylene diamine tetraacetic acid (EDTA) and dissolved organic matter (DOM)) on Hg toxicity to Photobacterium phosphoreum were evaluated in standardized 15 min acute toxicity tests . The significant increase of 6.3-fold in EC 50 data with increasing pH was observed over the tested pH range of 5.0–8.0, which suggested the possible competition between hydroxyl and the negatively charged chloro-complex. By contrast, it was found that major cations (Ca 2+ , Mg 2+ and K + ) have little effect on Hg toxicity to P. phosphoreum . An interesting finding was that the addition of HPO 4 2− significantly increased Hg toxicity, which may imply that the addition of phosphate increased the soluble Hg–chloro complex species. Additions of complexing agents (EDTA and DOM) into the exposure water increased Hg bioavailability via complexation of Hg. Finally, a model which incorporated the effect of pH, HPO 4 2− , HCO 3 − , SO 4 2− and DOM on Hg toxicity was developed to predict acute Hg toxicity for P. phosphoreum , which may be a useful tool in setting realistic water quality criteria for different types of water.
研究领域:
环境生物学
·
毒理学
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产品关联
DOI
PubMed
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-12
·
IF 4.259
Wang Dali,Gu Yue,Zheng Min,Zhang Wei,Lin Zhifen,Liu Ying
The determination of the chronic toxicity is time-consumed and costly, so it’s of great interest to predict the chronic toxicity based on acute data. Current methods include the acute to chronic ratios (ACRs) and the QSTR models, both of which have some usage limitations. In this paper, the acute and chronic mixture toxicity of three types of antibiotics, namely sulfonamides, sulfonamide potentiators and tetracyclines, were determined by a bioluminescence inhibition test. A novel QSTR model was developed for predicting the chronic mixture toxicity using the acute data and docking-based descriptors. This model revealed a complex relationship between the acute and chronic toxicity, i.e. a linear correlation between the acute and chronic lg(−lgEC50)s, rather than the simple EC 50 s or −lgEC 50 s. In particular, the interaction energies (E bind ) of the chemicals with luciferase and LitR in the bacterial quorum sensing systems were introduced to represent their acute and chronic actions, respectively, regardless of their defined toxic mechanisms. Therefore, the present QSTR model can apply to the chemicals with distinct toxic mechanisms, as well as those with undefined mechanism. This study provides a novel idea for the acute to chronic toxicity extrapolation, which may benefit the environmental risk assessment on the pollutants.
研究领域:
环境科学
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毒理学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2023
·
IF 8.4
Rongbing Li,Jiangtao Ren,Jinxing Chen,Ling Yang,Zhixuan Yu,Yunhui Li,Dengbin Yu,Youxing Fang,Shaojun Dong
In this study, a high-efficiency colorimetric sensor for water toxicity assessment was successfully constructed by the visual analysis with naked eyes and the high-throughput measurement with a microplate reader. The bioluminescent Photobacterium phoshoreum T3 spp. (T3) and Prussian blue (PB) were used as model bacterium and indicator of the colorimetric sensor, respectively. In order to accurately detect acute toxicities of wastewater, the experimental conditions, concentrations of cells, K 3 [Fe(CN) 6 ] and NaCl , reaction temperature and time, were optimized to be OD 600 = 2, 8 mM, 1.2%, 26.5 °C and 25 min, respectively. Then several samples of simulated wastewater containing Hg 2+ , Cd 2+ , Zn 2+ , 3,5-dichlorophenol (DCP) and formaldehyde (HCHO) were tested. The composite biotoxicity of dry battery leachate and real water sample was also evaluated. The IC 50 values of Hg 2+ , Cd 2+ , Zn 2+ and DCP obtained were 3.60, 1.56, 0.96 and 11.77 mg L −1 , respectively. Moreover, a toxic unit (TU) model was applied to evaluate the binary complex toxicity of Hg 2+ + Cd 2+ , Hg 2+ + Zn 2+ and DCP + Zn 2+ , which showed antagonistic effects. The work demonstrates that the promising high-efficiency sensor can detect quickly and sensitively the single and joint toxicities of multi-pollutants in water, which possesses extensive potentials in field of toxicity detection.
研究领域:
环境科学
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生物技术
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产品关联
DOI
高影响因子
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
·
2018
·Vol. 25
·期 26
·26504-26511
·
IF 2.8
Wei Sheng,Wang Fenghe,Chen Yajun,Lan Tao,Zhang Shengtian
Antibiotics and phthalate esters are two kinds of emerging pollutants and are ubiquitous in the aquatic ecosystem. To date, few studies analyzed the combined toxicity of the mixtures of antibiotics and phthalate esters, and their joint toxicity effect mode remains unknown. Here, we investigated the single and joint toxicity of dibutyl phthalate (DBP) and five antibiotics, namely, oxytetracycline hydrochloride (OTC), chlortetracycline hydrochloride (CTC), sulfamethazine (SMZ), sulfamerazine (SMR), and sulfadiazine (SD), to luminescent bacteria of Vibrio fischeri . The median effect concentration (EC 50 ) values of the test chemicals were ranked as CTC (6.67 mg/L) > OTC (25.12 mg/L) > SD (67.61 mg/L) > SMR (141.51 mg/L) > DBP (148.38 mg/L) > SMZ (245.07 mg/L). The joint toxicities of the binary mixtures of antibiotics and DBP were evaluated by the concentration addition (CA) and independent action (IA) models. The joint toxicity effects of CTC-DBP, OTC-DBP, SMZ-DBP, SMR-DBP, and SD-DBP all appeared to be synergism. Our study revealed that sulfonamides combined with DBP could be as toxic as or even more toxic than tetracycline. Thus, the joint toxicity effect should be considered when assessing the ecological risks of binary or multicomponent pollutants.
研究领域:
环境科学
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毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
International Journal of Environmental Research and Public Health
·
2018
·Vol. 15
·期 7
·1520
·
IF 2.145
Zhuqiu Sun,In-Sun Kang,Qianyuan Wu,Jinying Xi,Hongying Hu
Ultraviolet (UV) photodegradation is increasingly applied to control volatile organic compounds (VOCs) due to its degradation capabilities for recalcitrant compounds. However, sometimes the UV photodegradation products are also toxic and can affect human health. Here, 10 VOCs at 150~200 ppm in air were treated using a laboratory-scale UV reactor with 185/254 nm irradiation, and the biotoxicity of their off-gas was studied by investigating their off-gas absorption solutions. The CO2increase and VOC decrease were 39~128 ppm and 0~42 ppm, respectively, indicating that the VOCs and their products were mineralized in off-gas absorption solutions. The total organic carbon (TOC) of the absorption solutions are 4~20 mg∙L−1. Luminescent bacteria andDaphnia magnawere used to detect the acute toxicity, and anumuassay was used to determine the genotoxic potential. Trichloroethylene showed a highest toxicity to luminescent bacteria, while chlorobenzene had the lowest toxicity. Water-soluble UV photodegradation products for styrene are very toxic toDaphnia magna. In theumuassay, the genotoxicities of off-gas absorption solutions of trichloroethylene, methylbenzene, ethyl acetate, butyl alcohol, and styrene were 51.26, 77.80, 86.89, 97.20, and 273.62 mg (4-NQO)·L−1respectively. In addition, the analysis of the genotoxicity/TOC and intermediates products indicated that the off-gas absorption solutions of styrene, trichloroethylene, and butyl alcohol contain many highly toxic substances.Keywords:VOCs;UV photodegradation;biotoxicity;luminescent bacteria;Daphnia magna
研究领域:
环境科学
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毒理学
✓ 已验证
DOI
PubMed
高影响因子
CHEMOSPHERE
·
2015
·
IF 3.34
Shuang Ding,Junwei Wu,Meng Zhang,Huifeng Lu,Qaisar Mahmood,Ping Zheng
Acute toxicities of anaerobic ammonia oxidation (ANAMMOX) substrates and four antibiotics from pharmaceutical wastewaters on ANAMMOX process were reported. Individual and joint acute toxicity assays were performed using 50% inhibitory concentration (IC 50 ). Results showed that IC 50 values and their 95% confidence interval of ammonium chloride (A), sodium nitrite (B), penicillin G-Na (C), polymyxin B sulfate (D), chloramphenicol (E) and kanamycin sulfate (F) were 2708.9 (2247.9–3169.9), 1475.4 (1269.9–1680.9), 5114.4 (4946.4–5282.4), 10.2 (1.8–18.6), 409.9 (333.7–486.1) and 5254.1 (3934.4–6573.8) mg L −1 respectively, suggesting toxicities were in the order of D > E > B > A > C > F. Joint acute toxicities of bicomponent mixtures A and B, C and D, C and F, D and F were independent; D and E, E and F were additive while C and E were synergistic. Joint acute toxicities of multicomponent mixtures were synergistic or additive. Luminescent bacteria test is an easy and robust method for forecasting the feasibility of ANAMMOX process for pharmaceutical wastewater treatment .
研究领域:
环境科学
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微生物学
✓ 已验证
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2025
·
IF 8.2
Yue Zhang,Luqi Niu,Kai Jin,Yi Huang,Yuwei Zhong,Ting Pan,Jinliang Liu,Xiaohui Zhu,Yihan Wu
Photodynamic therapy (PDT) is a clinically effective antitumor treatment offering spatiotemporal precision, minimal invasiveness, and low systemic toxicity. However, its application is limited by the shallow penetration of external light sources, particularly for deep-seated tumors. Moreover, PDT alone often fails to fully inhibit tumor growth, prompting its combination with other modalities such as photothermal therapy (PTT), which induces tumor cell death through localized heating. In this study, we developed a nano-bacteria biohybrid, EcN-QS-iLux@Pd@MC540, by biomineralizing the engineered probiotic Escherichia coli Nissle 1917 (EcN-QS-iLux) with palladium nanoparticles and loading it with the photosensitizer MC540. This biohybrid performs self-driven PDT without external light and effective PTT under 808 nm light excitation. In vivo studies using a 4T1 tumor mouse model demonstrated complete tumor inhibition through the synergistic action of PDT and PTT, with no significant systemic toxicity. This platform showcases the use of nano-bioluminescent bacteria to integrate self-driven PDT with synergistic PTT, providing a targeted and minimally invasive strategy for cancer therapy.
研究领域:
医学
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生物工程
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癌症治疗
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产品关联
DOI
PubMed
高影响因子
CHEMICAL RESEARCH IN CHINESE UNIVERSITIES
·
2024
·Vol. 40
·期 2
·287-292
·
IF 3.1
Li Yaohua,Wang Wei
Intracellular pH (pH i ) is a fundamental indicator of cellular physiological state, regulating cellular state and function, and has important research values. Although various probes for measuring intracellular pH were available, it is challenging to reflect pH i in real-time and reversible manners. Herein, we developed a whole-cell bioluminescent (BL) probe based on wild type BL bacteria, photobacterium phosphoreum ( P. phosphoreum ), to determine and image pH i . The dependence of BL intensity of P. phosphoreum on pH values of culture solutions was established. It was found that BL intensity could respond to the change of pH values rapidly and reversibly. We further revealed that P. phosphoreum maintained pH homeostasis in the extracellular pH (pH e ) within the range of 5.0–7.0, while intracellular pH homeostasis was destroyed at the alkaline pH e . This method opens up the enormous potential of BL bacteria as an alternative to fluorescence for monitoring and imaging pH i .
研究领域:
生物化学
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细胞生物学
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生物物理学
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微生物学
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生物医学工程
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DOI
高影响因子
CHEMOSPHERE
·
2019
·
IF 5.108
Xiaoxian Zhang,Zhifen Lin
Guidelines of ecological risk assessment (ERA) used worldwide, based on S-shaped threshold dose-response curve, fail to consider hormesis, a biphasic dose-response model represented as a J-shaped or an inverted U-shaped curve, that occurs in real-life environment. Now that humans are routinely exposed to chemicals below the threshold where hormetic stimulation prevails, it is noteworthy that over-strictness about chemical control also means a waste of limited resources. So hormesis leads to the gap between guidelines with S-shaped model and reality with hormesis model concerning ERA. In this study, hormetic effects of sulfachloropyridazine (SCP) on the bioluminescence of Aliivibrio fischeri ( A. f ) under 41 conditions to simulate the real environment were investigated and compared with ERA practice by some parameters, such as no observed effect concentration (NOEC), hormetic-stimulatory range (HSR) and goal concentration (GC). Not only is the reproducibility of hormesis in real-life contexts confirmed, binomial distribution (p = 0.644 > 0.05) of the relative position of GC and HSR is also found, revealing a 50% probability for GC to falls in HSR, which proves the over-strictness of ERA both qualitatively and quantitatively. This study provides a novel view for ERA that hormetic principles should dominate, and conditions where S-shaped dose-response model works should be singled out on a specific basis to bridge the hormesis-induced gap.
研究领域:
环境科学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NanoImpact
·
2025
·
IF 5.5
Xinyi Liao,Zhihao Xue,Yunbu Dai,Jie Hou,Daohui Lin,Luqing Zhang
Micro/nanoplastics (MNPs) commonly coexist with contaminants such as heavy metals and antibiotics in aquatic environments, potentially inducing complex joint toxicity. However, conventional approaches for MNP-pollutant combined toxicity assessment are typically challenged by inefficiencies, high costs, and labor-intensive procedures. In this study, a high-throughput platform using microplate-based inhibition assays with a luminescent bacterium ( Vibrio qinghaiensis sp. - Q67), was applied to systematically evaluate the combined toxicity of four polystyrene nanoplastics (PS-NPs), varying in size and surface functionalization, with seven heavy metals and five antibiotics. Results showed that metal cations (Cu 2+ , Zn 2+ , Pb 2+ , Cd 2+ , and Cr 3+ ) and chlortetracycline combined with PS-NPs primarily induced antagonistic or additive toxicity, whereas anions ( Cr 2 O 7 2 − and As O 2 − ) and antibiotics (tetracycline, oxytetracycline, norfloxacin, and ciprofloxacin) exhibited synergistic or additive toxicity. Smaller PS-NPs (50 nm) amplified the toxicity interactions compared to 100 nm PS-NPs. Carboxyl-modified PS-NPs exhibited the most pronounced antagonistic effects with cations, while amino-modified PS-NPs favored amplified synergistic effects with Cr 2 O 7 2 − . The combined effects were intensified at higher PS-NP concentrations and at moderate co-contaminant concentrations (i.e., 1/5 or 1 × the median effect concentration). Antibiotics with higher water solubility exhibited more pronounced synergistic effects with PS-NPs, and the antagonism intensities for metal cations followed an order of Pb 2+ > Cu 2+ > Cr 3+ > Zn 2+ > Cd 2+ . This high-throughput strategy efficiently assessed multifactorial impacts on combined toxicity, providing systematic insights into the toxic interaction patterns between MNPs and pollutants.
研究领域:
环境毒理学
·
纳米技术
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水生生态学
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微生物学
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生态毒理学
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分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOCONJUGATE CHEMISTRY
·
2024
·
IF 4
Yanian Xiong,Yingtung Lo,Huizhu Song,Jianzhong Lu
The penetration ability of visible light (<2 mm) and near-infrared (NIR) light (∼1 cm) remarkably impairs the therapeutic efficacy and clinical applications of photodynamic therapy (PDT). To address the limitation of light penetration depth, a novel self-luminescent bacterium, teLuc.FP-EcN, has been engineered through transfection of a fusion expression plasmid containing the luciferase gene teLuc and bright red fluorescent protein mScarlet-I into Escherichia coli Nissle 1917 (EcN). The engineered teLuc.FP-EcN can specifically target and colonize tumors without significant toxicity to the host. Acting as a continuous internal light source, teLuc.FP-EcN can activate the photosensitizer chlorin e6 (Ce6) to generate reactive oxygen species (ROS) and then effectively destroy tumor tissue from the inside. As a result, a significant reduction in tumor proliferation and extension of the overall survival in mouse tumor models has been observed. Furthermore, teLuc.FP-EcN-boosted PDT amplified its therapeutic effect by activating antitumor immune response, including the conversion of M2 macrophages into pro-inflammatory M1 macrophages, as well as an increase in the proportion of CD3+ T cells and a decrease in T-cell exhaustion. In conclusion, teLuc.FP-EcN can be used as an implantable light source for tumor phototherapy, which simultaneously possesses ROS generation and immune regulation.
研究领域:
生物技术
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癌症研究
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治疗学
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免疫学
·
分子生物学
✓ 已验证
DOI
PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2024
·
IF 8.2
Jiacheng Sun,Bo Yan,Hongxing Chen,Shuchen Tu,Junhao Zhang,Tao Chen,Qinzi Huang,Yuting Zhang,Lingtian Xie
Currently, risk assessment and pollution management in mines primarily focus on toxic metals, with the flotation agents being overlooked. However, the combined effects of metals and flotation agents in mines remain largely unknown. Therefore, this study aimed to evaluate the combined effects of Cd and two organic flotation agents (ethyl xanthate (EX) and diethyldithiocarbamate (DDTC)), and the associated mechanisms. The results showed that Cd + EX and Cd + DDTC exhibited synergistic toxicity. The EC 50 values for luminescent bacteria were 1.6 mg/L and 1.0 mg/L at toxicity unit ratios of 0.3 and 1, respectively. The synergistic effects were closely related with the formation of Cd(EX) 2 and Cd(DDTC) 2 micro/nano particles, with nano-particles exhibiting higher toxicity. We observed severe cell membrane damage and cell shrinkage of the luminescent bacteria, which were probably caused by secondary harm to cells through the released CS 2 during their decomposition inside cells. In addition, these particles induced toxicity by altering cellular levels of biochemical markers and the transcriptional levels of transport proteins and lipoproteins, leading to cell membrane impairment and DNA damage. This study has demonstrated that particulates formed by Cd and flotation agents contribute to the majority of the toxicity of the binary mixture. This study helps to better understand the complex ecological risk of inorganic metals and organic flotation agents in realistic mining environments.
研究领域:
环境科学
·
毒理学
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矿物加工
·
生态毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
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