Nanoscience and Nanotechnology Letters
·
2017
·Vol. 9
·期 6
·941-944
·
IF 1.889
Nie Libo,Liu Fuhua,Yang Hongcheng
CdTe quantum dots (QDs) encoded polystyrene (PS) microbeads were synthesized in this article. Two or three kinds of QDs with different volume ratios were mixed. The PS microbeads were conjugated with the mixed QDs based on the coupling reaction of the carboxyl groups of QDs and the
amino groups on the surface of PS microbeads. The prepared QDs encoded PS beads shown specific fluorescence emission wavelengths and fluorescence intensities, which could be used as the support of specific bio-recognition in liquid biochips.
研究领域:
材料科学
·
生物技术
·
分析化学
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DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Xiaoshuang Zhao,Jieying Zhang,Yifan Chen,Ziting Chen,Zhe Liu,Yi Xu,Xingjun Zhu,Yang Li,Xianqiang Mi
With the rapid development of precise medicine, microbeads-based fluorescence bio-coding technology for multiplexed detection has attracted increasing attentions. However, traditional multiplexed bio-analysis could not meet the demand for multiplexed detection due to the limitations of labeling dyes and microscope channels. Here, a novel fluorescence encoding strategy was proposed by using multi-color quantum dots (QDs) acted as fluorescence tags and micron sized superparamagnetic particle acted as encoding carrier. By adjusting the color and number of labeled QDs, fluorescence encoded superparamagnetic particles (FESPs) with distinct fluorescence colors and intensities were constructed. The FESPs were prepared by metal coordination-based layer-by-layer assembly with excellent binding effect. By binding DNA capture oligonucleotides, FESP probes were constructed for multiplexed detection of miRNA-21, miRNA-122 and miRNA-130b with excellent specificity. Moreover, the miRNA targets could be easily separated within 5 s originated from the excellent superparamagnetism, enabling great simplified bio-analysis process. The results demonstrated here will provide opportunities to realize the full potentials of fluorescence bio-coding in biomedical detection and diagnosis.
研究领域:
生物医学工程
·
分子诊断学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Xiaolong Qiao,Linlin Li,Qiulian Liu,Yuanming Zhang,Guangting Han,Haoxi Ben,Haiguang Zhao,Wei Jiang
The traditional textile degumming process produces abundant wastewater, which contains a lot of monosaccharides and oligosaccharides. It is of great economic and environmental significance to utilize these carbohydrates in high value. In this study, high performance liquid chromatography (HPLC) was used to analyze the carbohydrate components in kenaf degumming wastewater, and then the production of C-dots using the wastewater was explored. The results showed that the types and content in the degumming wastewater were monosaccharides (glucose, xylose and arabinose) and oligosaccharides (dextran, xylan and araban). The carbohydrate (mainly glucan and xylan) content in wastewater accounted for 91.16 % of the total carbohydrates weight loss in kenaf degumming process. By using hydrolysis and hydrothermal reaction on kenaf degumming wastewater, blue-green carbon dots (C-dots) with good performance were prepared and successfully applied to anti-counterfeiting printing. In particular, the as-prepared C-dots prepared from kenaf degumming wastewater with urea added (WUC-dots) showed an excitation-dependent photoluminescence (PL) spectrum and quantum yield (QY) of 2.4 % in aqueous solution. The fluorescent code exhibited a clear outline, excitation-tunable color and good stability, showing a great potential for anti-counterfeiting system.
研究领域:
环境科学
·
材料科学
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2024
·
IF 18.5
Jing Guo,Ruihui Liu,Yuezi Lv,Hui Xie,Shaobing Zhou
Shape-morphing hydrogels can mimic the dynamism of living creatures and are popular for designing soft machines, such as actuators and robots. However, most existing shape-morphing hydrogels present a single morphing pathway between two shapes, sequential and multistage shape-morphing in real scenarios has rarely been captured. Although a strategy that assembles functional hydrogel components has been reported, it is likely to simultaneously increase the complexity and weaken the versatility of hydrogel machines. Here, a light-encoding strategy based on the dynamic coordination between the ferric iron and carboxyl group (Fe 3+ ─COO − ) to integrate the frame and actuating units into a single hydrogel without assembly is proposed. The spatiotemporal control of light irradiation is expected to make the light-encoding process sequential and reprogrammable. Results demonstrate that the light-encoded patterns determine the morphing route and further activate shape-morphing owing to swelling mismatch. The actuating units can be successively created by localized irradiation or elaborately turned by re-coordinating and light-encoding. This endows the encoded hydrogel with sequential and multistage shape-morphing behavior, similar to the living creatures. This strategy allows the design of assembly-free soft machines with sequential and multistage shape-morphing performance, which will benefit the design of more types of hydrogel machines.
研究领域:
材料科学
·
生物工程
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产品关联
DOI
高影响因子
NANOTECHNOLOGY
·
2019
·Vol. 31
·期 6
·065101
·
IF 3.399
Qing-Yi Zhuang,Xiao-Hui Wang,Zhao-Xin Geng,Hong-Shang Peng
In this study, fluorescence-encoded magnetic biocompatible nanoparticles (NPs) were constructed from CdSe@ZnS quantum dots (QDs) and Fe3O4 nanoparticles with a one-step reprecipitation-encapsulation method. The resultant hybrid NPs exhibit small size (∼130 nm in diameter), highly bright QDs, two-color emissions (green and red) under single-wavelength excitation, easy separation with a magnet and efficient cellular internalization. Energy transfer between the incorporated QDs was studied to better tailor the encoded fluorescence, and 11 barcodes were obtained by adjusting the ratio of green and red QDs. We used four sets of the barcodes to tag specific cancer cells (HepG2) as a proof-of-concept, and distinguished each set according to respective overlayed fluorescence images using laser confocal microscopy. Moreover, the incorporated Fe3O4 NPs endowed as-constructed optical barcode superparamagnetic property by T2-enhanced magnetic resonance effect with an r2 value of 145.25 s−1 mM−1 at 3 T. These results suggest that the multifunctional NPs are very promising for discriminating different cells and dual-modality imaging.
研究领域:
生物医学工程
·
纳米医学
·
分子影像学
✓ 已验证
DOI
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2024
·Vol. 48
·期 43
·18429-18436
·
IF 2.7
Yan Zhuang,Kai Gong,Jianhui Sun,Zishi Jiang,Yiqian Li,Peng Li
Traditional silica-based optical fiber strain sensors suffer from a limited range of strain detection, mismatched mechanical performance, and complicated sensing structures that hinder their practical applications involving complex strain patterns and large strain measurement. To solve these issues, the development of novel flexible optical fiber strain sensors with distributed and multimodal strain sensing performance is critical but challenging. In this work, a flexible hydrogel optical fiber with a step-index core/cladding structure was successfully designed and fabricated using a simple thermal polymerization method involving calcium alginate and polyacrylamide. The resulting flexible fiber exhibited a large stretch deformation capacity and good optical transmission property as well as an effective and reversible strain response. Significantly, a flexible hydrogel functional fiber was further constructed by doping quantum dots with different fluorescent emission wavelengths in different positions. By exploiting a combination of emission wavelength encoding and transmission loss, the assembled flexible hydrogel functional optical fibers could distinguish and recognize the locations, magnitudes, and modes (stretch or bend) of mechanical deformation through a single wavelength of visible light (365 nm) excitation. An ultra-large dynamic range (0–120%) of axial strain and bending deformation (10°–80°) could be effectively detected. This work offers a versatile solution to develop promising stretchable and distributed fiber strain sensors.
研究领域:
材料科学
·
光学工程
·
生物医学工程
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产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Cheng-Yu Li,Di Cao,Chu-Bo Qi,Ya-Feng Kang,Chong-Yang Song,Dang-Dang Xu,Bei Zheng,Dai-Wen Pang,Hong-Wu Tang
Establishment of a stable analytical methodology with high-quality results is an urgent need for screening cancer biomarkers in early diagnosis of cancer. In this study, we incorporate holographic optical tweezers with upconversion luminescence encoding to design an imageable suspension array and apply it to conduct the detection of two liver cancer related biomarkers, carcinoembryonic antigen and alpha fetal protein. This bead-based assay is actualized by forming a bead array with holographic optical tweezers and synchronously exciting the upconversion luminescence of corresponding trapped complex beads fabricated with a simple one-step sandwich immunological recognition. Owing to the fact that these flowing beads are stably trapped in the focal plane of the objective lens which tightly converges the array of the laser beams by splitting a 980 nm beam using a diffraction optical element, a fairly stable excitation condition is achieved to provide reliable assay results. By further taking advantage of the eminent encoding capability of upconversion nanoparticles and the extremely low background signals of anti-Stokes luminescence, the two targets are well-identified and simultaneously detected with quite sound sensitivity and specificity. Moreover, the potential on-demand clinical application is presented by employing this approach to respond the targets toward complex matrices such as serum and tissue samples, offering a new alternative for cancer diagnosis technology.
研究领域:
生物医学工程
·
癌症诊断技术
·
光学成像
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产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry C
·
2015
·Vol. 3
·期 24
·6314-6321
·
IF 4.696
Huan Chen,Yanbo Lang,Yongling Zhang,Dan Zhao,Guanshi Qin,Changfeng Wu,Kezhi Zheng,Weiping Qin
The demands for multiplexed biological detection have driven the development of fluorescence encoding nanoprobes. We have synthesized water-soluble dual mode emission core–shell rare earth nanoprobes (∼30 nm) for fluorescence encoding. The nanoprobes were composed of ytterbium (Yb), erbium (Er) and/or thulium (Tm) ions co-doped heterogeneous NaYF4/NaLuF4 nanocrystals as cores and amorphous SiO2 embedded with europium (Eu) or terbium (Tb) complexes as shells. Excited by both infrared light and ultraviolet light, the nanoprobes exhibited dual characteristic emissions, which enable a novel spectral encoding strategy. The core nanocrystals exhibited tunable up-conversion emissions through various lanthanide ions doping. Combining these multiple upconversion emissions of core nanocrystals with downconversion emissions of shell containing rare earth complexes, a large amount of distinct fluorescence codes can be generated.
研究领域:
生物检测
·
纳米材料科学
·
光学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED SURFACE SCIENCE
·
2020
·
IF 6.182
Lijuan Sun,Yichen Zhang,Zhinan Fan,Chi Zhang,Li-Juan Fan
Fluorescence-encoded microspheres are largely demanded in high-throughput assays. A strategy for preparing encoded microspheres based on fluorescence resonance energy transfer (FRET) was advanced. Two fluorophores, poly(phenylenevinylene) nanoparticles (PPV NPs) as the donor and rhodamine 6G (R6G) as the acceptor were employed to construct the energy-transfer pair, and introduced onto the substrate polymeric microspheres. The fluorescence intensity ratio between PPV NPs and R6G molecules were tuned based on energy transfer mechanism. Nine codes (wavelength-intensity combinations) were obtained at the same excitation by keeping the concentration of PPV NPs constant and varying the concentration of R6G in the dipping solutions. Then, a melamine–formaldehyde (MF) resin layer was coated onto the encoded spheres to prevent the leakage of fluorophores. The encoded spheres were found to have good fluorescence stability against various solvents and even “dissociation agent“, and no significant variation of the codes were observed. Further modification of this MF layer with silanization reaction provided the amino reactive sites. In all, this study provides a general strategy for the preparation of fluorescence encoded microspheres based on energy transfer as well as a stable protective layer with reactive sites.
研究领域:
生物化学
·
材料科学
·
分析化学
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产品关联
DOI
高影响因子
Journal of Materials Chemistry C
·
2015
·Vol. 3
·期 31
·8262-8271
·
IF 4.696
Yan Li,Youshen Wu,Cheng Luo,Bo Wang,Daocheng Wu
Rewritable magnetic fluorescence-encoded microspheres were prepared by coating magnetic microspheres with a fluorescence-encoded melamine-formaldehyde (MF) shell. Fluorescence encoding was realized by varying the dye types and concentrations incorporated into the MF shell. Ten sets of magnetic fluorescence-encoded microspheres including four sets doped with single dye and six sets doped with two dyes were obtained and successfully identified through flow cytometric analysis. High encoding capacity could be achieved by increasing the dye number and intensity levels. More importantly, the original fluorescence-encoded MF shell could be removed via calcination. The inner magnetic microspheres remained unaffected and could be re-coated with a new fluorescence-encoded MF shell easily. The fluorescent MF coating (encoding writing) and calcination (encoding erasing) composed a cycle. Results demonstrated that the magnetic microspheres retained their original characteristics after writing–erasing cycles and the writing–erasing cycle could be repeated numerous times. These kinds of rewritable magnetic fluorescence-encoded microspheres enabled recycling and regeneration. Combining magnetic separation and re-encoding, which can significantly simplify the analysis processes and decrease the analysis cost, these microspheres are expected to be widely used in suspension arrays for high-throughput analyses.
研究领域:
材料科学
·
化学
·
生物传感
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2025
·
IF 14.1
Jie Zhao,Xinyi Liu,Dan Li,Xuefei Jing,Weijie Wu,Liangrui He,Yizhang Tang,Zhen Liu,Yulin Zeng,Haitian Sha,Huibin Qiu,Xujiang Yu,Wanwan Li
Constructing colloidal particles into designated hierarchies with integrated functionalities offers insights into bottom-up material fabrication. However, the arbitrary and controllable assembly of individual nanoscale and microscale particles remains highly challenging due to their diverse structural properties. Here, a general paradigm for the robust co-assembly of plasmonic noble metal nanoparticles and multifunctional polymer microspheres through thermodynamically driven heterocoagulation and coordination interactions is developed. Monodisperse poly(styrene-co-maleic anhydride) microspheres (PSMA MSs) are facilely synthesized using a one-step emulsification method with optional simultaneous encapsulation of magnetic and fluorescent nanoparticles. Upon surfactant removal, the purified PSMA MSs become metastable and trigger ultrafast co-assembly of silver nanocubes (AgNCs) onto MSs within 5 min with the maximum coverage rate reaching nearly 50%. The densely immobilized AgNCs outside magnetic MSs exhibit excellent catalytic efficiency (>89% conversion across 15 cycles) and enable enzyme integration for cascade reactions. Moreover, the largest reported plasmonic-fluorescent encoded array (124 codes) is realized, achieving a significantly improved detection limit of 10 copies/reaction for respiratory viruses. This work addresses the long-standing challenge of achieving uniform distribution and dense immobilization of plasmonic noble metal nanoparticles and offers a general approach to fabricating designated hierarchies based on noble metal nanoparticles, with integrated functionalities for high-performance practical applications.
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产品关联
DOI
PubMed
高影响因子
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