WATER RESEARCH
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2025
·
IF 12.4
Yan Cai,Xiaoci Li,Peizeng Yang,Jing Chen,Junhe Lu,Jean-Marc Chovelon,Yuefei Ji
As one of benzophenone-derived drugs, benzbromarone (BBM) has been widely used to reduce blood uric acid, treat gout, and gouty arthritis. Understanding the transformation and fate of BBM in natural and engineered systems is critical for its ecological risk assessment. In this study, we systematically investigated the photochemical behavior of BBM in aqueous solutions under laboratory UV 254 irradiation. UV-vis spectra show that an aqueous solution of BBM is capable of absorbing UV photons at 200 - 400 nm. Spectroscopic titration indicates that BBM with a p K a value of 4.83 ± 0.17 is present mainly as the phenolate form under circumneutral conditions. BBM undergoes rapid direct photolysis when exposed to UV 254 irradiation and the quantum yields were determined to be 0.0105 and 0.0196 mol E −1 for phenol and phenolate forms, respectively. The heavy atom effect of bromine and spin-orbit coupling effect of aromatic ketone make dibromophenol and carbonyl moieties the critical chromophores accounting for the high photoreactivity of BBM. Laser flash photolysis and electron paramagnetic resonance studies suggest that the photolysis of BBM is initiated by ultrafast photodebromination and Norrish I cleavage. The high yield of bromide determined by ion chromatograph highlights the importance of photodebromination. Due to the light screening effect of wastewater components, the photolysis of BBM in hospital wastewater is inhibited. Photo-induced modification of the dibromophenol moiety of BBM likely generates photoproducts showing toxicity to luminescent bacteria. Overall, our results reveal that photochemical reaction under UV irradiation plays an important role in the attenuation of BBM in engineered water.
研究领域:
环境科学
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药理学
·
毒理学
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DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2019
·Vol. 29
·期 28
·1901058
·
IF 15.621
Zenghe Liu,Luzhi Zhang,Qingbao Guan,Yifan Guo,Jiaming Lou,Dong Lei,Shuliang Wang,Shuo Chen,Lijie Sun,Huixia Xuan,Eric Meade Jeffries,Chuanglong He,Feng-Ling Qing,Zhengwei You
Natural tissues possess superior material properties such as self-healing, mechanical robustness, and mechanical gradients that allow organisms to adapt and survive in dangerous environments. Although highly desired, imparting synthetic materials with these biomimetic protective features remains a challenge. Here, the versatile dimethylglyoxime–urethane (DOU) moiety is used to create a multifunctional polyurethane (DOU-PU). The reactivities of DOU including reversible dissociation, metal coordination, photolysis enabled self-healing, high strength and toughness, mechanical gradient formation, and spatially controlled functionalization. By incorporating DOU, a multifunctional protective film is produced with superior resistance to mechanical damage, rapid room temperature self-healing, and anti-counterfeiting features. This super biomimetic film is expected to be very useful for the protection of various types of valuable objects such as electronics, diplomas, currency, and automobiles.
研究领域:
材料科学
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生物医学工程
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DOI
高影响因子
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
·
2021
·
IF 4.291
Miao Yao,Shaohui Liu,Chen Huang,Jun Nie,Yong He
Five ester derivatives from hydroxyalkyl of 2-hydroxy-2-methyl-1-phenyl propanone, one of the most successfully applied α-hydroxyalkyl acetophenone photoinitiators, are synthesized. From the spectroscopy results and theory calculation, it is proved that the much lower photocleavage rates of the directly linked unsaturated ester derivatives are due to that the strong electron withdrawing effect of carbonyl increase the bond strength between the benzoyl and quaternary carbon and the effective energy transfer from benzoyl to double bond because of the existence of p-σ-π hyperconjugation, which consequently decreased their initiating ability. Excitingly, simple introduction of two-methylene spacer between the carbonyl and alkoxyl can effectively block the hyperconjugation and significantly increase the photocleavage rate up to the same level of their mother commercial photoinitiator, which can open a gate for design of new high efficiency and polymerizable type I photoinitiators.
研究领域:
有机化学
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材料科学
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光化学
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DOI
高影响因子
International Journal of Nanomedicine
·
2019
·
IF 4.471
Junhui Shi,Zhengyan Zhao,Zongjun Liu,Ruozheng Wu,You Wang
Background: The design of novel nanoparticles with higher therapeutic efficacy and lower side effects, is still difficult but encouraging in cancer therapy. Specifically, for upconversion nanoparticles (UCNP)-based drug release, a high intensity of NIR light (1.4~5.0 W/cm2) above the maximum permissible exposure (0.33 W/cm2 for 980 nm) is commonly used and severely limits its practical application.Methods: The highly emissive UCNP is first synthesized and then coated with mesoporous silica (MS) shell (UCMS). Next, the surface of UCMS is modified with the thioether (-S-BP) linker, leading to UCMS-S-BP nanoparticles. Finally, after the drug doxorubicin (Dox) is loaded into the pore channels of UCMS, the pore openings are blocked by the β-cyclodextrin (β-CD) gatekeeper through the association with the -S-BP linker (UCMS(Dox)-S-BP@β-CD).Results: Upon 980 nm NIR light irradiation with an ultralow intensity of 0.30 W/cm2, it is found that the loaded Dox can be released through the cleavage of thioether linkers triggering dissociation of β-CD gatekeepers. The in vitro results exhibited significantly therapeutic efficacy with 85.2% of HeLa cells killed in this study.Conclusions: An ultralow-intensity NIR light triggered on-demand drug release system has been developed by employing highly emissive UCNP and photocleavable linker with low bond dissociation energy to avoid the potential photodamage on healthy neighbor cells.
研究领域:
纳米医学
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肿瘤学
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光化学
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DOI
PubMed
高影响因子
LANGMUIR
·
2015
·
IF 4.457
Ruidong Cheng,Min Tian,Sujuan Sun,Chenghui Liu,Youpeng Wang,Zhaotie Liu,Zhongwen Liu,Jinqiang Jiang
The amphiphilic random copolymer of P(NVP-co-NHPSS) with photocleavable N–O sulfonate side groups has been prepared to investigate the light-triggered disruption of copolymer micelles. Methods of absorption and emission spectra, solution transmittance, dynamic light scattering (DLS), and transmission electron microscopy (TEM) were applied. It was found that P(NVP-co-NHPSS) could form polymeric nanoaggregates in aqueous solution. And the photocleavage of the N–O bond within copolymer micelles upon 365 nm UV light could be conveniently controlled by changing the irradiation intensity, leading to the disruption of copolymer micelles and the photocontrolled release of Nile red encapsulation. And by encapsulating NaLuF4:Gd/Yb/Tm UCNPs inside copolymer micelles, the response of the photocleavable N–O bond to the 980 nm laser was much weaker than the response to 365 nm light; however, the photocontrolled release of Nile red could still be effectively triggered by the NIR light of the 980 nm laser.
研究领域:
材料科学
·
化学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF MEDICINAL CHEMISTRY
·
2022
·
IF 8.039
Sijin Liu,Xi Zhao,Sufang Shui,Biao Wang,Yingxian Cui,Suwei Dong,Tairan Yuwen,Guoquan Liu
Targeted degradation of proteins, especially those regarded as undruggable or difficult to drug, attracts wide attention to develop novel therapeutic strategies. Glutathione peroxidase 4 (GPX4), the key enzyme regulating ferroptosis, is currently a target with just covalent inhibitors. Here, we developed a targeted photolysis approach and achieved efficient degradation of GPX4. The photodegradation-targeting chimeras (PDTACs) were synthesized by conjugating a clinically approved photosensitizer (verteporfin) to noninhibitory GPX4-targeting peptides. These chimeras selectively degraded the target protein in both cell lysates and living cells upon red-light irradiation. The targeted photolysis of GPX4 resulted in dominant ferroptotic cell death in malignant cancer cells. Moreover, the dying cells resulting from the PDTACs exhibited potent immunogenicity in vitro and efficiently elicited antitumor immunity in vivo. Our approach therefore provides a novel method to induce GPX4 dysfunction based on noncovalent binding and specifically trigger immunogenic ferroptosis, which may boost the application of ferroptosis in cancer immunotherapy.
研究领域:
癌症生物学
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免疫治疗
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药理学
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细胞生物学
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DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2021
·Vol. 33
·期 4
·1100-1108
·
IF 3.665
Kaiqi Li,Xiliang Liu,Long Chen,Zuochun Xiong,Chengdong Xiong,Dongliang Chen
Amino-functionalized six-membered cyclic carbonate 5-((2-nitrobenzyl) amino)-1,3-dioxan-2-one (NBAC) was synthesized with 2-nitrobenzyl group as photolabile protecting group. The following copolymerization was carried out by using l -lactide as comonomer and Sn (Oct) 2 as catalyst. The amino-functionalized polymer P(LA- co -AC) was obtained by ultraviolet irradiation of P(LA- co -NBAC) to remove the protecting group. The structures of products were confirmed by 1 H nuclear magnetic resonance (NMR) and 13 C NMR analysis. The pendant amino groups not only resulted in an enhancement of the hydrophilicity, but also played a role of antibacterial effect due to the positive charge imparted to the material.
研究领域:
材料科学
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生物医学工程
·
有机化学
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Songkuan Zhuang,Xiaojun Huang,Muyan Diao,Ruiling Dong,Yueyu Dong,Jun Liu,Yizhen Liu
Current one-pot CRISPR diagnostics necessitate meticulous control of nucleic acid hybridization parameters or extensive modification of CRISPR components to achieve complete enzymatic silencing, a fundamental bottleneck limiting their robustness and generalizability. Here, we resolve this challenge through dynamic crRNA reconfiguration, a paradigm-shifting strategy that exploits the intrinsic structural duality of CRISPR RNA. We present CONVERT (CRISPR One-Pot Nucleic acid detection Via Engineered crRNA Reconfiguration Technology), a universal platform where nontarget intact crRNA acts as a universal suppressor, achieving complete Cas12a inhibition during RPA (recombinase polymerase amplification) by irreversible enzyme sequestration. Target detection is initiated through programmable conversion to split crRNA activators, bypassing sequence-specific design constraints entirely. This crRNA conformational switching mechanism is implemented via photocleavable linker cleavage and subsequent assembly with presupplied truncated crRNA (tcrRNA), activating trans-cleavage signal amplification. Three transformative advances emerge: First, the endogenous crRNA engineering eliminates dependency on exogenous nucleic acid blockers or chemical modifications, reducing optimization costs and time. Second, near-total enzymatic suppression ensures zero cross-talk between amplification and detection phases. Third, the unified workflow enables contamination-resistant operation through spatiotemporal control, requiring no physical partitioning. Validated for Enterocytozoon bieneusi detection, the system demonstrates attomolar sensitivity of 1 aM, 100% diagnostic concordance with qPCR, and absolute specificity against related pathogens. By effectively decoupling CRISPR regulation from sequence-context constraints through rational crRNA structural plasticity, this work establishes a generalized framework for next-generation molecular diagnostics.
研究领域:
分子生物学
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生物工程
·
诊断技术
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合成生物学
·
核酸扩增
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF INORGANIC BIOCHEMISTRY
·
2023
·
IF 3.9
Xiaolong Peng,Qiang Tang,Huiyun Zhu,Lijuan Bai,Hua Zhao,Yongjie Chen
Three ruthenium arene complexes, namely {[(η 6 - p -cymene)Ru(Cl)] 2 (dpb)}(PF 6 ) 2 ( 1 ), [(η 6 - p -cymene)Ru( dpb )Cl](PF 6 ) ( 2 ) and [(η 6 - p -cymene) Ru( dpb )py](PF 6 ) ( 3 ) (dpb = 2,3-bis(2-pyridyl)benzo-quinoxaline, py = pyridine), were synthesized and their antitumor properties were introduced. Complexes 1-3 were characterized by 1 H NMR, MS, and elemental analysis . As a binuclear ruthenium structure, the absorption of metal ligand electron transfer (MLCT) of 1 extended to 700 nm. Complex 1 was significantly hydrolyzed under dark conditions. The cytotoxicity in vitro study showed that complexes 1 and 2 are more toxic to human lung cancer cells (A549) and human cervial cancer cells (Hela) than cisplatin . Moreover, there was almost no cross-resistance between complex 1-2 and cisplatin. Under the irradiation at 478 nm, complexes 1-3 all produced singlet oxygen ( 1 O 2 ), and the 1 O 2 quantum yield of complex 1 in PBS is the highest among complexes 1-3 . Complex 1 also produced 1 O 2 under 600 nm light irradiation . DNA gel electrophoresis showed that 1 caused the photocleavage of plasmid DNA. The hydrolysis rate of complex 1 was accelerated under light (λ > 600 nm). And the phototoxicity of complex 1 to Hela cells under light (λ > 600 nm) was much greater than its dark toxicity, which may be due to its generation of 1 O 2 and the promotion of its hydrolysis under long-wave light irradiation.
研究领域:
化学
·
药理学
·
肿瘤学
·
光化学
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产品关联
DOI
PubMed
高影响因子
Journal of Radiation Research and Applied Sciences
·
2020
·
IF 1.804
Xue-Wen Liu,Ning-Yi Liu,Yuan-Qing Deng,Shan Wang,Ting Liu,Yu-Cai Tang,Yuan-Dao Chen,Ji-Lin Lu
ABSTRACT In recent years, ruthenium (II) complexes have attracted attention due to high DNA topoisomerase I inhibitory activities and good anticancer activities. Herein, an asymmetric ligand 2-(5-(1, 10-phenanthroline))-1 H-4,5-diphenyl-imidazole (pdpi) and its ruthenium complexes were synthesized. DNA-binding mode of two complexes was determined as intercalation by using UV–vis spectra, emission spectra, viscosity, and molecular docking experiments. DNA photocleavage experimental results show that two ruthenium complexes effectively cleave plasmid DNA by producing singlet oxygen. Furthermore, they both displayed good topo I inhibition activities. The cytotoxic activities results show that complex 2 displays better antitumor activities against Eca-109 cells and A549 cells (IC 50 = 25.97 ± 3.33 μM and 28.83 ± 2.49 μM, respectively) compared to complex 1 .
研究领域:
化学
·
药理学
·
癌症研究
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DOI
高影响因子
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