CHEMICAL ENGINEERING JOURNAL
·
2023
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IF 15.1
Ruoyu Deng,Dongxu Yang,Mengli Chen,Qiang He,Qingjuan He,Yi Chen
Nonthermal plasma has attracted extensive attention because of its ability to remove emerging organic micropollutants. However, its high amount of energy consumption severely limits its economic feasibility. In this study, sulfadiazine degradation was achieved in an optimized dielectric barrier discharge (DBD)-Contact system. The sulfadiazine removal in the DBD-Contact/peroxymonosulfate (PMS) system reached 91.8% within 15 min. Compared to a conventional DBD system, the energy efficiency (G 50 ) of the DBD-Contact/PMS system was 4.37 times greater, which meant a saving of 77.17% in electricity consumption. The reactive species generated by discharge plasma were identified as mostly excited nitrogen species in the gas phase, and after transferred to the water phase, OH and SO 4 − played an important role in the sulfadiazine removal. The hydroxylation of the S-N bond and the nucleophilic reaction of the pyrimidine ring were proposed as mechanisms for the sulfadiazine degradation, and the developmental and acute toxicity was significantly reduced after treatment. Furthermore, the DBD-Contact/PMS system showed good applicability in a wide pH range and complex practical water samples. This study finds that the DBD-Contact/PMS system can degrade antibiotics with high efficiency and low energy consumption and provides new insights for the practical application of DBD technology in water treatment.
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JOURNAL OF ETHNOPHARMACOLOGY
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2023
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IF 5.4
Qin Tang,Zhengcai Ma,Xiang Tang,Yan Liu,Huimin Wu,Yu Peng,Baihua Jiao,Rui Wang,Xiaoli Ye,Hang Ma,Xuegang Li
Ethnopharmacological relevance Helicobacter pylori (H. pylori) is a major pathogen colonized in the human stomach and is implicated in gastritis, peptic ulcer, and gastric carcinoma. Antibiotics are useful for eradicating H. pylori but failed for drug resistance, making it urgent to develop effective and safe drugs. Rhizoma Coptidis was reported as one of the most effective Chinese medicines to treat H. pylori -related gastrointestinal diseases, while the precise antimicrobial mechanism remains unclear. Thus, it is of great significance to study the antimicrobial ingredients and corresponding mechanisms of Rhizoma Coptidis. Aim of the study To search for the most effective alkaloid against H. pylori in Rhizoma Coptidis and illustrate the probable mechanisms . Materials and methods Five main alkaloids in Rhizoma Coptidis were isolated. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were tested to determine the most effective one. Bacterial growth experiments, Annexin V-FITC/PI staining, TUNEL staining, and transmission electron microscopy (TEM) were performed to further study the anti- H. pylori activity of coptisine (Cop) . The in vivo effect of Cop on H. pylori eradication rate and H. pylori -induced inflammation was investigated in mice . Transcriptomics was used to understand the underlying mechanism of eradicating H. pylori and reducing host inflammation. Western blot, RT-PCR, and ELISA experiments were utilized and confirmed that cagA was one of the targets of Cop. Results According to the MIC and MBC, Cop was the most effective alkaloid against H. pylori , especially with no drug resistance developed . In vitro experiments showed that Cop inhibited H. pylori by inducing DNA fragmentation, phosphatidylserine exposure, and membrane damage. Cop (150 mg/kg/day) effectively eradicated H. pylori in mice and reduced the levels of IL-2 and IL-6 to relieve gastric inflammation. Transcriptomic analysis revealed that virulence factor cagA was one of the hub genes associated with the inflammation-improving effect of Cop. That is, Cop could decrease the expression of CagA and subsequently reduce the translocation of CagA to gastric epithelial cells, thereby improving the morphology of hummingbird-like phenotype induced by CagA and alleviating inflammation . Conclusions Cop is the most effective alkaloid in Rhizoma Coptidis and might act through multiple mechanisms for H. pylori eradication along with reducing the expression of CagA to alleviate inflammation.
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Algal Research-Biomass Biofuels and Bioproducts
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2023
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IF 5.1
Chong Yu,Chi Li,Ying Zhang,Xiang Du,Jing-Han Wang,Zhan-You Chi,Qian Zhang
Attached cultivation of microalgae using mariculture effluent could simultaneously realize cost-effective biomass production and effluent polishing, and the obtained microalgal biofilm could be readily applied to aquatic animals that feed directly on biofilms. However, the widespread existence of antibiotics in mariculture effluent may have inhibitory effects on microalgae. Existing studies regarding the influences of antibiotics on microalgae were overwhelmingly mostly conducted in suspended freshwater cultures, and the explored antibiotic concentrations were several orders of magnitudes higher than realist effluent, rendering limited guidance for practical applications. This study, therefore, studied three commonly observed antibiotics in mariculture effluent, i.e. sulfamethoxazole (SMX), tetracycline (TL), and clarithromycin (CLA), and explored their effects on attached seawater Chlorella sp. growth, nutrients removal, and antioxidative properties at environment-relevant concentrations (0.1–200 μg/L). Hormesis was shared among the three antibiotics, and highest biofilm density (14.6 g/m 2 ) was observed under 0.1 μg/L SMX. SMX displayed strongest chlorophyll impairment, while SMX, TL, and CLA respectively favored lipid (up to 42.4 %), protein (up to 39.6 %), and polysaccharides (up to 65.4 %) accumulation. Highest correlation between biofilm density and extracellular polymeric substances (EPS) contents as well as highest total nitrogen (TN) removal were observed under TL exposure, while total phosphorus (TP) removal among all groups met the strictest local discharge standard for mariculture effluent (TP < 0.2 mg/L). Antioxidative responses revealed least stress towards attached seawater Chlorella sp. under TL treatment, while best acclimation under SMX exposure. Potential coping mechanisms of this microalgae under each antibiotic exposure were proposed as well. This study expands the knowledge of microalgae biofilm under environment-relevant concentrations of antibiotics.
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ANALYTICAL LETTERS
·
2023
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IF 2
Xiaoyu Zhang,Peiran Meng,Wei Wang,Xiwen Li,Zuan Yang,Shaokai Du,Yue Sun
Simultaneous determination of erythromycin (Ery) and hemoglobin (Hb) is of great significance for clinical diagnosis of many diseases. ZnO nanorods modified with Au nanoparticles were fabricated as an interface on the flexible carbon cloth (CC) and utilized to simultaneously determine Ery and Hb by a simple electrochemical method. The results demonstrated that Au/ZnO/CC displayed excellent analytical performance for Ery with a low detection limit of 3.38 × 10−7 mg/L and a linear range from 1 × 10−6 to 1 × 10−1 mg/L. At the same time, the sensor also showed a sensitive response to Hb with a low detection limit of 2.07 × 10−7 mg/L and a linear range from 1 × 10−6 to 1 × 10−1 mg/L. This work provides a viable approach for designing a flexible electrode and sensitive simultaneous determination of Ery and Hb.
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Matter
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2022
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IF 19.967
Yu Wang,Yunxiang He,Qin Wang,Xiaoling Wang,Blaise L. Tardy,Joseph J. Richardson,Orlando J. Rojas,Junling Guo
The wide-spread overuse and misuse of antibiotics has led to major risks to human health, which demands breakthrough technologies for elimination of antibiotics from water streams. Membrane-based water purification has drawn substantial interest for this purpose. However, high permeance and high antibiotic-removal efficiency remain extremely challenging. In this work, the use of polyphenol-based nanoengineering to functionalize conventional microporous membranes capable of ultrafast removal of ten different antibiotics in an in-line flowthrough purification system is explored. The high adsorption kinetics of these nanocoatings enable a record-high permeance (9,774 L m −2 h −1 bar −1 ) with exceptional removal rate and efficiency, at a relatively low energy cost (0.09 kWh m −3 ), even in a real-world wastewater treatment. Molecular dynamics simulations provide detailed insights into the role of polyphenol-based nanocoatings and their multiple molecular interactions with antibiotics. This work provides a promising and sustainable platform for engineering the next-generation adsorption-based membranes for clean water production.
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ANALYTICA CHIMICA ACTA
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2022
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IF 6.911
Jian Zhang,Zhijun Bao,Jingjing Qian,Haibo Zhou,Kui Zhang
In this work, copper-doped zinc sulfide quantum dots (ZnS:Cu QDs) were developed as ratiometric fluorescent probes to detect tetracycline (TC) in milk. With the increasing of TC concentration, the fluorescence at 578 nm of ZnS:Cu QDs was quenched, while a new fluorescence at 520 nm was significantly enhanced due to the formation of a green-emitting complex of Zn 2+ and TC on the surface of probes. The fluorescence intensity ratio (I 520 /I 578 ) have a good linear relationship (R 2 = 0.98) with the concentration of TC in the range of 0.2–2.0 μM. The detection limit was calculated to 149 nM, which is lower than the allowable level of TC (about 180 μM) in milk set by the US Food and Drug Administration (FDA). Finally, the fluorescent test papers have been also prepared using ZnS:Cu QDs as ink by painting on a piece of filter paper. With the addition of TC, the orange test paper produced green spots that became more and more obvious with a dose-discerning ability as low as 2 μM. This work demonstrated a great potential of ratiometric fluorescent probe for the easy and visual detection of TC in complicated real samples.
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EUROPEAN JOURNAL OF PHARMACOLOGY
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2022
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IF 5.195
Wan-Feng Xu,Yun Wang,Hai Huang,Jia-Wei Wu,Yuan Che,Chu-Jie Ding,Quan Zhang,Wei-Ling Cao,Li-Juan Cao
Gastritis is a common inflammation of stomach with multiple pathogenesis. This study was designed to investigate the protective effects of oral octreotide (OCT) against ethanol-induced acute gastric injury and H. pylori -induced chronic gastritis via promoting gastric mucosa restoration, reducing gastric acid secretion and inflammation. Male C57BL/6J mice were randomly divided and treated with three doses of OCT (0.5, 2.5, 10 mg/kg) alone or combined respectively with 10 mg/kg omeprazole (OME), 0.2 g/L metronidazole (MTZ)/0.1 g/L clarithromycin (CLR) in drinking water. Oxidative stress analysis, bacterial load analysis, qPCR, gastric histopathology examinations were performed in our study. Ethanol-induced acute gastric ulcer was restored by OCT alone at doses of 2.5 mg/kg, or combined with OME as indicated by markedly reducing Gastrin , Il-6 and Il1b expression through induction of Muc5ac and Occludin , significantly improving hyperacidity and gastric bleeding . As well, OCT combined with MTZ/CLR restored the integrity of gastric mucosa damaged by H. pylori via elevating the expression of Muc5ac and somatostatin receptor 2 , decreasing inflammation and increasing the number of chorionic or glands. Besides, OCT is more suitable for long-term medication in the treatment of chronic gastritis than OME. In conclusion, our results proved that the newly developed oral OCT-based therapies were more effective to reverse gastric mucosa damage and inflammation in ethanol and H. pylori infection-induced gastric injury, it is of great significance for supplementing new clinical regimens for the treatment of acute and chronic gastritis.
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Journal of Inflammation Research
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2022
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IF 4.631
Ling Li,Yaoyao Du,Yang Wang,Ning He,Bing Wang,Tong Zhang
Background Gastric ulcer (GU) is the most common multifactor gastrointestinal disorder affecting millions of people worldwide. There is evidence that gut microbiota is closely related to the development of GU. Atractylone (ATR) has been reported to possess potential biological activities, but research on ATR alleviating GU injury is unprecedented.Methods Helicobacter pylori (H. pylori)-induced GU model in zebrafish and ethanol-induced acute GU model in rat were established to evaluate the anti-inflammatory and ulcer inhibitory effects of ATR. Then, 16S rRNA sequencing and metabolomics analysis were performed to investigate the effect of ATR on the microbiota and metabolites in rat feces and their correlation.Results Therapeutically, ATR inhibited H. pylori-induced gastric mucosal injury in zebrafish. In the ulceration model of rat, ATR mitigated the gastric lesions damage caused by ethanol, decreased the ulcer area, and reduced the production of inflammatory factors. Additionally, ATR alleviated the gastric oxidative stress injury by increasing the activity of superoxide dismutase (SOD) and decreasing the level of malondialdehyde (MDA). Furthermore, ATR played a positive role in relieving ulcer through reshaping gut microbiota composition including Parabacteroides and Bacteroides and regulating the levels of metabolites including amino acids, short-chain fatty acids (SCFAs), and bile acids.Conclusion Our work sheded light on the mechanism of ATR treating GU from the perspective of the gut microbiota and explored the correlation between gut microbiota, metabolites, and host phenotype.
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DALTON TRANSACTIONS
·
2023
·Vol. 52
·期 19
·6551-6558
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IF 4
Wenjuan Dong,Lu Wang,Rongrong Zhang,Chaochao Wen,Ren Su,Xiaojuan Gong,Wenting Liang
Carbon dots are popular luminescent materials because of their excellent fluorescence properties, but the low quantum yield limits their application. Heteroatom doping is a more convenient and popular approach to increase the quantum yield of carbon dots. Here, novel N,S,P heteroatom co-doped carbon dots (N,S,P-CDs) were synthesized by a simple one-step hydrothermal method using m-phenylenediamine, L-cysteine and phosphoric acid as raw materials. The as-prepared N,S,P-CDs showed excellent photoluminescence properties with a fluorescence quantum yield of up to 41%, which greatly encourages their application in fluorescence sensing. The N,S,P-CDs exhibited good fluorescence stability under salt solution, xenon lamp irradiation and ultraviolet lamp irradiation except for a high sensitivity to extreme acidity. The fluorescence intensity of the N,S,P-CDs can be decreased by as much as 85% when the pH of the solution changes from 2.50 to 4.75, that is, a small fluctuation in pH can cause an intense response of the fluorescence of the N,S,P-CDs. Therefore, an excellent fluorescence sensing platform for accurately monitoring the pH of extreme acidity has been constructed. In addition, the N,S,P-CDs can be applied for quantitative detection of folic acid based on the strong quenching effect of folic acid on the fluorescence of the N,S,P-CDs. Good linearity was obtained in the concentration range of 4.85–82.45 μM, with a detection limit of 0.148 μM. The constructed sensing platform was used for the determination of folic acid in actual samples of orange juice, oatmeal and tablets with satisfactory results.
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ACS Applied Nano Materials
·
2021
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IF 5.097
Li Zhang,Huaizhong Xing,Wei Liu,Zihan Wang,Yumin Hao,Huiping Wang,Wenjuan Dong,Yang Liu,Shaomin Shuang,Chuan Dong,Xiaojuan Gong
A ratiometric optical dual-mode probe was first designed for the detection of doxorubicin (DOX) based on 11-mercaptoundecanoic acid-functionalized carbon dots (MUA-CDs). The carbon dots (CDs) were synthesized via hydrothermal carbonization of m-phenylenediamine and p-aminobenzoic acid, and then it was reacted with 11-mercaptoundecanoic acid by the amide reaction to obtain the MUA-CDs. The as-synthesized MUA-CDs have a high fluorescence quantum yield (59.4%) with bright green emission. Based on a specific electrostatic interaction and the fluorescence resonance energy transfer (FRET) between MUA-CDs and DOX, a ratiometric optical dual-mode probe was constructed for the detection of DOX with good selectivity, high sensitivity, and excellent anti-interference performance. When DOX was mixed the MUA-CDs, the fluorescence of the MUA-CDs at 513 nm decreased, while the fluorescence at 590 nm increased. With this information, a ratiometric fluorescent method was established to determine DOX with a linear range and limit of detection (LOD) of 0.25–19.96 and 0.66 μM and 50.33–80.88 and 0.23 μM, respectively. Meanwhile, the color of the MUA-CDs varied from colorless to dark yellow in the presence of DOX, and therefore, a ratiometric colorimetric method was applied to determine DOX in a range of 2.50–29.80 μM with a LOD of 0.75 μM. Finally, the as-constructed probe was successfully used to determine DOX in serum, urine, and live cells with satisfactory results. This work will contribute to the specific design of functional CDs and broaden the application of functional CDs in analytical detection and biological imaging.
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ACS Nano
·
2023
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IF 17.1
Huahai Yu,Ying Piao,Yifan Zhang,Jiajia Xiang,Shiqun Shao,Jianbin Tang,Zhuxian Zhou,Youqing Shen
Effective accumulation and penetration of antibiotics in the biofilm are critical issues for bacterial infection treatment. Red blood cells (RBCs) have been widely utilized to hitchhike nanocarriers for drug delivery. It is vital and challenging to find a nanocarrier with an appropriate affinity toward RBCs and bacteria for selective hitchhiking and release that determines the drug delivery efficiency and specificity. Herein, we report a zwitterionic polymer poly(2-(N-oxide-N,N-diethylamino)ethyl methacrylate) (OPDEA)-based micelle, which can hitchhike on RBCs in blood and preferentially release in the infection site. We found that OPDEA could bind to the RBCs cell membrane via phospholipid-related affinity and transfer to Gram-positive bacteria due to nearly an order of magnitude stronger interaction with the bacteria cell wall. The zwitterionic surface and cell-wall affinity of OPDEA-based micelles also promote their penetration in biofilm. The clarithromycin-loaded OPDEA micelles show efficient drug delivery into the infection site, resulting in excellent therapeutic performance in both peritonitis and pneumonia models by intravenous or spray administration. This simple RBC-selective hitchhiking and releasing antibiotic delivery system provides a promising strategy for the design of antibacterial nanomedicines.
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ENVIRONMENTAL RESEARCH
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2021
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IF 6.498
Shuting Zeng,Jing Sun,Ziwei Chen,Qiuxiang Xu,Wei Wei,Dongbo Wang,Bing-Jie Ni
Clarithromycin retained in waste activated sludge (WAS) inevitably enters the anaerobic digestion system. So far, the complex impacts and fate of clarithromycin in continuous operated WAS anaerobic digestion system are still unclear. In this study, two semi-continuous long-term reactors were set up to investigate the effect of clarithromycin on biogas production and antibiotic resistance genes (ARGs) during WAS anaerobic digestion, and a batch test was carried out to explore the potential metabolic mechanism. Experimental results showed that clarithromycin at lower concentrations (i.e., 0.1 and 1.0 mg/L) did not affect biogas production, whereas the decrease in biogas production was observed when the concentration of clarithromycin was further increased to 10 mg/L. Correspondingly, the relative abundance of functional bacteria in WAS anaerobic digestion (i.e., Anaerolineaceae and Microtrichales ) was reduced with long-term clarithromycin exposure. The investigation of ARGs suggested that the effect of methylation belonging to the target site modification played a critical role for the anaerobic microorganisms in the expression of antibiotic resistance, and ermF , played dominated ARGs, presented the most remarkable proliferation. In comparison, the role of efflux pump was weakened with a significant decrease of two detected efflux genes. During WAS anaerobic digestion, clarithromycin could be partially degraded into metabolites with lower antimicrobial activity including oleandomycin and 5-O-desosaminyl-6-O-methylerythronolide and other metabolites without antimicrobial activity.
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危险品化学品经营许可证（带存储）