Food Analytical Methods
·
2026
·Vol. 19
·期 10
·303
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IF 3
Tu Xiaodong,Yao Mingcai,Dong Liang
A chloramphenicol (CAP) electrochemical sensor based on zinc tungstate and maghemite nanocomposites (ZnWO 4 /γ-Fe 2 O 3 ) is proposed. First, ZnWO 4 and γ-Fe 2 O 3 nanomaterials were synthesized using a hydrothermal synthesis method, then compositely modified onto glassy carbon electrodes to construct electrochemical sensors for measuring CAP. Under optimal conditions, the ZnWO 4 /γ-Fe 2 O 3 -modified electrode exhibits a stronger reduction peak current for CAP detection than the single-component electrode and demonstrates better electrocatalytic activity. The results demonstrate that ZnWO 4 /γ-Fe 2 O 3 /GCE exhibits a linear detection range of 8–1000 μM and a detection limit of 1.04 μM, demonstrating good selectivity in the presence of different interferers, as well as outstanding stability and reproducibility. ZnWO 4 /γ-Fe 2 O 3 /GCE was used to detect of CAP in milk samples, yielding satisfactory results.
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PHYSIOLOGY & BEHAVIOR
·
2022
·
IF 3.742
Yan Sun,Haiyan Zhu,Rongrong Cheng,Zhengquan Tang,Min Zhang
Akkermansia muciniphila is present in the mucus layer of its host gut, and its outer membrane protein Amuc_1100 has a significant ameliorative effect on metabolic disorders and emotional memory aspects of enteritis, obesity, depression, and anxiety in the host. Antibiotics affect gut microbial composition, leading to imbalance and behavioral changes in the gut-brain axis, while probiotics have a protective effect against behavioral changes caused by gut flora disorders. In the present study, a depressed mouse model using a broad-spectrum cocktail mixture resulted in increased anxiety and depression-like behavior, decreased serum and hippocampal levels of 5-hydroxytryptamine (5-HT), and increased serum corticosterone (cort) levels. After application of A. muciniphila and Amuc_1100, anxiety and depression-like behavior in antibiotic-treated mice were significantly alleviated. In addition, the brain derived neurotrophic factor / Tropomyosin receptor kinase B (BDNF/TrkB) signaling pathway was altered, glial fibrillary acidic protein (GFAP) expression increased, and c-Fos protein expression decreased in the hippocampus of antibiotic-treated mice. After treatment with A. muciniphila and Amuc_1100, BDNF and TrkB levels were restored in the hippocampus and cortex. These results suggest that A. muciniphila and Amuc_1100 may alleviate antibiotic-induced anxiety and depression by affecting the BDNF/TrkB signaling pathway.
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Computational Intelligence and Neuroscience
·
2022
·
IF 3.12
Guiqiu Li,Ying Wei,Yan Guo,Hui Gong,Jie Lian,Guangjian Xu,Bing Bai,Zhijian Yu,Qiwen Deng
This study aimed to evaluate the activity, resistance, clonality of MIC distribution, and the correlation between virulence and resistance genes and biofilm formation of omadacycline (OMC) in clinics for Streptococcus agalactiae isolates from China. 162 isolates were collected retrospectively in China. The S. agalactiae were collected from the body’s cervical secretions, wound secretions, ear swabs, secretions, semen, venous blood, cerebrospinal fluid, pee, etc. The MIC of OMC against S. agalactiae was determined by broth microdilution. The inhibition zone diameters of OMC and other common antibiotics were measured using filter paper. D-test was performed to determine the phenotype of cross resistance between erythromycin and clindamycin. In Multilocus sequence typing (MLST), some commonly-detected resistance genes and virulence gene of these S. agalactiae isolates were investigated using polymerase chain reaction (PCR). Biofilms were detected by crystal violet staining. Our data demonstrated the correalation of the biofilm formation and OMA antimicrobial susceptibility of S.agalactiae clinical isolates with the carrier of virulence gene scpB. Conclusively, OMC exhibits the robust antimcirobial activity against clinical S. agalactiae isolates from China compared with DOX or MIN, and the carrier of the virulence gene scpB might correlate with the biofilm formation in OMC-resistant S. agalactiae.
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Foods
·
2021
·Vol. 10
·期 8
·1838
·
IF 4.35
Yu Wang,Jin-Yi Yang,Ying He,Lu Li,Jian-Xin Huang,Yuan-Xin Tian,Hong Wang,Zhen-Lin Xu,Yu-Dong Shen
Tylosin and tilmicosin (T&T) residues in livestock products have received extensive attention from consumers. Time-resolved fluorescence immunochromatographic assay (TRFICA), as a fast, efficient and sensitive immunoassay method, has played an increasingly important role in the food safety field. Therefore, herein a quantitative and visual TRFICA was established for simultaneously detecting T&T in milk in a group-screening manner. Under the optimal conditions, the standard curve range of developed TRFICA based on the T&T was 1.87~7.47 ng/mL, and the half-maximal inhibition concentrations (IC50) were 4.06 ng/mL and 3.74 ng/mL, respectively. The limits of detection (LOD) of the TRFICA method were from 1.72 ng/mL to 1.39 ng/mL, and the visual cut-off values were 31.25 ng/mL and 62.50 ng/mL for T&T in milk, respectively. Moreover, the stability experiments showed that the strips could be stored at 4 °C for more than 6 months, the total detection time was less than 13 min, and the cross-reactivities (CRs) with related compounds were less than 0.1%, which concluded that the developed TRFICA method could be used in real milk sample detection.Keywords:time-resolved fluorescence immunochromatographic assay;tylosin;tilmicosin;milk
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FOOD CHEMISTRY
·
2020
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IF 6.306
Zeqing Dong,Jian Lu,Yilin Wu,Minjia Meng,Chao Yu,Chang Sun,Muning Chen,Zulin Da,Yongsheng Yan
As a veterinary antibiotic, lincomycin (LIN) residues in milk are raising concerns of public on account of potential harm to human health. Efficient strategy is eagerly desired for detection of LIN from milk samples. Hence, lincomycin molecularly imprinted membranes (LINMIMs) were developed for selective separation of LIN as an efficient pretreatment of milk samples. The synergistic effect of polyethylenimine and dopamine provided effective antifouling performance by improving the hydrophilicity. Based on click chemistry, specific recognition sites were facilely formed on membranes using 4-vinylpyridine as functional monomers. The satisfactory rebinding capacity (151.62 mg g −1 ), permselectivity (4.43), together with the linear dependence ( R 2 = 0.9902) of concentrations in eluents and original samples. Moreover, the method was utilized to determine LIN from milk, with good recovery and relative standard deviation. Achievements in this work will actively promote the development of efficient detection technology.
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Matter
·
2022
·
IF 19.967
Yu Wang,Yunxiang He,Qin Wang,Xiaoling Wang,Blaise L. Tardy,Joseph J. Richardson,Orlando J. Rojas,Junling Guo
The wide-spread overuse and misuse of antibiotics has led to major risks to human health, which demands breakthrough technologies for elimination of antibiotics from water streams. Membrane-based water purification has drawn substantial interest for this purpose. However, high permeance and high antibiotic-removal efficiency remain extremely challenging. In this work, the use of polyphenol-based nanoengineering to functionalize conventional microporous membranes capable of ultrafast removal of ten different antibiotics in an in-line flowthrough purification system is explored. The high adsorption kinetics of these nanocoatings enable a record-high permeance (9,774 L m −2 h −1 bar −1 ) with exceptional removal rate and efficiency, at a relatively low energy cost (0.09 kWh m −3 ), even in a real-world wastewater treatment. Molecular dynamics simulations provide detailed insights into the role of polyphenol-based nanocoatings and their multiple molecular interactions with antibiotics. This work provides a promising and sustainable platform for engineering the next-generation adsorption-based membranes for clean water production.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Zhenping Liu,Jun Zhou,Xianfeng Wang,Jinsong Zhao,Peng Zhao,Yi Ma,Suyi Zhang,Danqun Huo,Changjun Hou,Kang Ren
In view of the urgent need for fluoroquinolones contamination detection in the fields of food safety, a novel aptasensor based on the fluorescence quenching property of graphene oxide (GO) and the fluorescence characteristic of cadmium selenide quantum dots (CdSe QDs) was developed for fluoroquinolones highly sensitive detection in this work. The CdSe QDs with carboxyl-rich surface were synthesized successfully and fluoresced at 525 nm under the optimal excitation light of 366 nm. Based on the hydrophobic and π-π stacking between GO and aptamer, aptamer labeled by CdSe QDs fluorescence (CdSe QDs-apt) were adsorbed by GO and the fluorescence of CdSe QDs was quenched. After the aptamer combined specifically with fluoroquinolones, greater specific force lead to the desorption of CdSe QDs-apt from GO and fluorescence recovery. Represented by Ciprofloxacin (CIP), a member of fluoroquinolones, the fluorescence emission increased with the increasing of CIP concentrations from 8 nM to 500 nM, and the detection limit was 0.42 nM. The spiked recoveries in real samples of honey and milk were 91.5–96.9 % and 90.3–95.2 %, respectively, indicating that the aptasensor was reliable. Moreover, the fluorescence responses of multiple members of fluoroquinolones were found to be consistent, denoting that the fluorescence aptasensor can be used to detect the total amount of multiple members of fluoroquinolones. These results showed that the aptasensor can be used as a promising platform for fluoroquinolones detection.
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ANALYTICA CHIMICA ACTA
·
2023
·
IF 6.2
Jutao Hu,Xiaoyu Su,Lei Yuan,Kaiyi Zheng,Xiaobo Zou,Zongbao Sun,Xuechao Xu,Wen Zhang
Accurate and sensitive detection of chloramphenicol (CAP) in natural samples is essential for ensuring human health. Herein, an enzyme-regulated fluorescence sensor using Fe 3 O 4 @COF/Fe 3+ probe, is developed for CAP determination. Fe 3 O 4 @COF, synthesized via hydrothermal method , exhibits dual functions as a magnetic carrier and signal probe. Bovine serum albumin conjugated-chloramphenicol, adsorbed on the surface of Fe 3 O 4 @COF, competes with CAP for antibody binding. The antibody interacts with alkaline phosphatase via the biotin-streptavidin system. Meanwhile, ascorbic acid , produced from the enzyme-catalyzed reaction dominated by alkaline phosphatase, effectively restores the fluorescence of Fe 3 O 4 @COF that is quenched by Fe 3+ . After experimental verification and gradual optimization, a logarithmic linear relationship between CAP concentration and fluorescence intensity is established in the range of 2 × 10 −4 ∼10 μg mL −1 , with a good limit of detection (9.2 × 10 −5 μg mL −1 ). Proposed method exhibits excellent stability (15 days) and reusability (8 cycles), providing a sensitive and reliable method for accurate CAP detection. The readouts show good agreement with HPLC and recoveries during laboratory and natural CAP analysis.
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ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
·
2023
·
IF 4.9
Xiaojun Hu,Renhan Dong,Sheng Huang,Yisheng Zeng,Wei Zhan,Xiaofang Gao,Dong Tian,Jian Peng,Jiewei Xu,Ting Wang,Yaying Zhang,Xiaohui Wang,Xiaoxia Zhang,Jin Liu,Bing Guang,Tai Yang
Clostridioides difficile infection (CDI) causes severe diarrhea and colitis, leading to significant morbidity, mortality, and high medical costs worldwide. Oral vancomycin, a first-line treatment for CDI, is associated with a high risk of recurrence, necessitating novel therapies for primary and recurrent CDI. A novel small-molecule compound, CDBN-YGXZ, was synthesized by modifying the benzene ring of nitazoxanide with lauric acid. The mechanism of action of CDBN-YGXZ was validated using a pyruvate:ferredoxin/flavodoxin oxidoreductase (PFOR) inhibition assay. The efficacy of CDBN-YGXZ was evaluated using the MIC test and CDI infection model in mice and hamsters. Furthermore, metagenomics was used to reveal the underlying reasons for the effective reduction or prevention of CDI after CDBN-YGXZ treatment. The inhibitory activity against PFOR induced by CDBN-YGXZ. MIC tests showed that the in vitro activity of CDBN-YGXZ against C. difficile ranging from 0.1 to 1.5 μg/mL. In the mouse and hamster CDI models, CDBN-YGXZ provided protection during both treatment and relapse, while vancomycin treatment resulted in severe relapse and significant clinical scores. Compared with global effects on the indigenous gut microbiota induced by vancomycin, CDBN-YGXZ treatment had a mild influence on gut microbes, thus resulting in the disappearance or reduction of CDI recurrence. CDBN-YGXZ displayed potent activity against C. difficile in vitro and in vivo, reducing or preventing relapse in infected animals, which could merit further development as a potential drug candidate for treating CDI.
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INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
·
2024
·
IF 5
Jun Huang,Gang Yang,Kewei Chen,Muying Du,Zsolt Zalán,Ferenc Hegyi,Jianquan Kan
Sterigmatocystin (STC) is an emerging mycotoxin that poses a significant threat to the food security of cereal crops. To mitigate STC contamination in maize, this study employed selected lactic acid bacteria as biocontrol agents against Aspergillus versicolor , evaluating their biocontrol potential and analyzing the underlying mechanisms. Lactiplantibacillus plantarum HJ10, isolated from pickle, exhibited substantial in vitro antifungal activity and passed safety assessments, including antibiotic resistance and hemolysis tests. In vivo experiments demonstrated that L. plantarum HJ10 significantly reduced the contents of A. versicolor and STC in maize (both >84 %). The impact of heat, enzymes, alkali, and other treatments on the antifungal activity of cell-free supernatant (CFS) was investigated. Integrated ultra-high-performance liquid chromatography (UPLC) and gas chromatography–mass spectrometry (GC–MS) analysis revealed that lactic acid, acetic acid, and formic acid are the key substances responsible for the in vitro antifungal activity of L . plantarum HJ10. These metabolites induced mold apoptosis by disrupting cell wall structure, increasing cell membrane fluidity, reducing enzyme activities, and disrupting energy metabolism. However, in vivo antagonism by L . plantarum HJ10 primarily occurs through organic acid production and competition for growth space and nutrients. This study highlights the potential of L . plantarum HJ10 in reducing A. versicolor and STC contamination in maize.
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WATER RESEARCH
·
2024
·
IF 11.4
Xiaodan Zheng,Menglin Wang,Shiqi Zhang,Zhima Yangcuo,Lifang He,Lingfang Xie,Yurou Ye,Guifen Xu,Zhonghui Chen,Qihong Cai
Antibiotics are a new type of environmental pollutants. Due to its wide application in many fields, antibiotic residues are ubiquitous in the wastewater environments. Given their potential threat on water ecosystem functioning and public health, the detection of antibiotic residues in wastewater environments has become very necessary. Based on the complexation of Al 3+ with flumequine (FLU), ciprofloxacin (CIP) and doxycycline hydrochloride (DOX), their molecular conjugated area were increased and fluorescence intensity were enhanced, combined with synchronous fluorescence spectrometry (SFS) had good selectivity and high sensitivity, a novel method of Al 3+ sensitized synchronous fluorescence spectrometry for the determination of FLU , CIP and DOX residues in wastewater was established. When the wavelength difference (Δλ) was selected 115.0 nm, synchronous fluorescence spectra of the three antibiotics could be well separated and the interference of wastewater matrix were eliminated primely. The new SFS made good use of spectral separation instead of conventional chemical separation, and the actual wastewater sample could be directly determined after simple filtration. The experiment results showed that the concentrations of FLU, CIP and DOX in the range of 0.5000–800.0 ng·mL -1 , 0.5000–640.0 ng·mL -1 and 10.00–3500 ng·mL -1 had a good linear relationship with fluorescence intensity. The detection limits of three antibiotics were 0.02054 ng·mL -1 , 0.03956 ng·mL -1 and 0.8524 ng·mL -1 , respectively. Recovery rates of three antibiotics in wastewater samples were 90.72%-98.23%, 88.68%-95.08% and 85.94%-96.70%. The new SFS established in this experiment had the advantages of simple, rapid, sensitive, accurate and good selectivity. Simultaneous and rapid detection of FLU, CIP and DOX residues in wastewater was successfully realized. It had good application prospects in real-time water quality monitoring.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yaowei Zhu,Ruijie Tang,Jun Zhao,Changlong Jiang,Lingfei Li,Bianhua Liu
Tetracyclines antibiotics (TCs) are widely used as broad-spectrum antibiotics, but their overuse leads to environmental pollution and threatens human health. Existing detection techniques (mass spectrometry, etc.) are complicated and cumbersome, and do not allow for rapid detection. Consequently, it is imperative to develop a sensing technology for rapid on-site detection of full-spectrum TCs residues. The UV–visible absorption peaks of TCs overlap with the blue fluorescence excitation wavelength range, which can “turn-off” the blue fluorescence based on the internal filtering effect. Additionally, the β-diketone bond of TCs can bind to Eu 3+ , sensitizing Eu 3+ to produce red fluorescence. Thus, we constructed a selective sensing chemo-fluorescent sensor using blue nitrogen-doped carbon dots (BCDs) and europium metal–organic framework (Eu-MOF). As the concentration of TCs increased from 0 to 2.6 μM, the sensor exhibited a continuous change in fluorescence color from blue to purple to red. The limit of detection is 1.37 nM with a response time of less than 1 s. Furthermore, this study presents a portable capillary chemo-fluorescent sensor detection kit, which consists of a capillary chemo-fluorescent sensor comprising BCDs/Eu-MOF encapsulated in a self-designed non-fluorescent glass capillary, as well as a color recognizer with a kit produced using 3D printing technology. This fluorescence sensing technology with excellent sensitivity and selectivity provides technical support for the rapid detection of TCs and the reduction of pollutant hazards to sustainable human development.
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