Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 15
·2591-2597
·
IF 4.872
Yilin Li,Hong Dai,Qingrong Zhang,Shupei Zhang,Sihong Chen,Zhensheng Hong,Yanyu Lin
A versatile photoelectrochemical immunoassay protocol was designed for quantitative monitoring of tumor markers by utilizing the poly(dopamine)-sensitized titanium dioxide (TiO2) signal crystal with an ordered mesoporous carbon support. Poly(dopamine) was introduced to alter the optical properties of the TiO2 signal crystal, thereby improving the visible light absorption and photoelectrical responses. More importantly, a new enzyme-like biomimetic catalyst was exploited as the signal amplifier to catalyze the reaction of hydroquinone. The generated product was deposited on the electrode surface and served as an efficient sacrificial electron acceptor, which could receive the photo-generated electrons of the excited semiconductor to assist the cathode photocurrent enhancement. Herein, a competitive-type immunosensor was achieved through the biomimetic catalyst labeled prostate specific antigen competing with the target antigen of different concentrations to react with the specific antibody anchored on the poly(dopamine)-sensitized TiO2 signal crystal. Under optimal conditions, the photocurrent decreased with increasing target concentration in a dynamic working range from 1 × 10−6 ng mL−1 to 50 ng mL−1, which provided a new photoelectrochemical method for tumor markers analysis.
研究领域:
免疫学
·
肿瘤学
·
分子生物学
·
生物传感器
·
转化医学
✓ 已验证
DOI
高影响因子
Nanoscale
·
2018
·Vol. 11
·期 3
·962-967
·
IF 7.233
Zhaohong Su,Xiaolin Xu,Yongbing Cheng,Yueming Tan,Langtao Xiao,Daili Tang,Hongmei Jiang,Xiaoli Qin,Huixian Wang
A porous graphene (PG) bionanocomposite of PG, gold nanoparticles (AuNPs) and anti-indole-3-acetic acid (anti-IAA) antibody for sensitive and label-free amperometric immunoassay of IAA was reported. A PG film was produced by a pre-reduction/electrochemical reduction process on a glassy carbon electrode (GCE) and then a homogeneous AuNPs layer electrodeposition on the PG film. The anti-IAA antibody was immobilized onto the AuNPs through electrostatic adsorption and covalent conjugation. Scanning electron microscopy (SEM), transmission electron microscopy (TEM), Raman spectroscopy, energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV), elecro-chemical impedance spectroscopy (EIS), ultraviolet visible spectroscopy (UV-vis) and differential pulse voltammetry (DPV) were used to characterize the PG film and the stepwise modification of the immunosensor. The electrochemical immunosensor exhibited a wide linear range from 2 × 10−11 to 2 × 10−8 g mL−1 with a detection limit of 0.016 ng mL−1 (S/N = 3) and showed significant linearity R2 = 0.9970. In addition, the proposed immunosensor showed acceptable selectivity and has been applied to the determination of IAA in the extract samples of several plant seeds with acceptable relative derivation (%) ranging from −5.25% to 4.24% between the immunosensor and high performance liquid chromatography. The proposed chemical pre-reduction and electro-reduction guided protocol can be extended to the preparation of many other functionalized PG nanocomposite films for wide applications.
研究领域:
生物传感
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纳米生物技术
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分析化学
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植物生理学
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电化学
·
免疫分析
✓ 已验证
DOI
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2019
·
IF 2.398
Zexiang Wang,Song Hu,Ganggang Zhang,Jintao Liu,Jun Xia,Juan Peng,Weihua Lai
Sulfamethazine (SMZ), widely used for disease prevention of bees, is harmful to consumers when excess SMZ residues are present in honey. In this study, a novel competitive lateral flow immunoassay (LFIA) was established by using aggregation-induced emission fluorescent microsphere (AIEFM) as label for the detection of SMZ. Results indicated that the limit of detection (LOD) for the developed competitive AIEFM-LFIA in honey was 0.028 ng/mL, and the corresponding linear range was 0.05−5 ng/mL. Recovery experiments showed that the average recovery range was 75.6–95.1% with coefficient variations of 5.7–12.9% in honey. The developed competitive AIEFM-LFIA was compatible with HPLC-MS/MS in detecting SMZ in 15 real samples from honey. In general, the proposed AIEFM-LFIA is well suited for the competitive format and has great potential for the detection of other small targets with single antigenic site in practical application.
研究领域:
食品安全
·
分析化学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
·
2011
·Vol. 26
·期 6
·1191-1197
·
IF 4.372
Jing-Min Liu,Xiu-Ping Yan
An ultrasensitive method for simultaneous determination of cytochrome c (cyt-c) and insulin was developed by combining aptamer-based bioassay and immunoassay, multielement-tagging and inductively coupled plasma mass spectrometry (ICP-MS). Aptamer-modified gold nanoparticles (apt-AuNPs) and antibody-modified silver nanoparticles (ab-AgNPs) were employed as specific element tags for cyt-c and insulin, respectively. The prepared surface-functionalized magnetic microparticles (MMPs) were used for efficient and fast magnetic separation. The bioassay conditions were carefully optimized, including the amount of MMPs, the concentration of AuNPs and AgNPs, and the reaction time. Under optimal conditions, the developed method gave a linear range of 0.1–20 nM for cyt-c and 0.2–40 nM for insulin, a detection limit (3s) of 1.5 fmol (30 pM in 50 μL) for cyt-c and 5.5 fmol (110 pM in 50 μL) for insulin. The precision (relative standard deviation) for six replicate determinations of cyt-c (0.6 nM) and insulin (2.0 nM) was 6.6%, and 6.0%, respectively. The present method exhibits good specificity with recoveries from 87% to 98% for spiked cyt-c and insulin in human serum samples. The methodology demonstrated here provides a new possibility for bioassays and clinical diagnoses, which has potential for simultaneous determination of two or more low-abundance biomarkers of interest via multi-element tags.
研究领域:
生物分析化学
·
分子诊断
·
纳米生物技术
·
临床检验医学
·
蛋白质组学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Huiqiang Wang,Zhanfang Ma
A new strategy for signal amplification was introduced in a label-free amperometric immunoassay platform. Improvements in the signal occurred following the oxidation of glucose by glucose oxidase and catalysis by polythionine of the produced H 2 O 2 from the previous oxidation reaction. In addition, a multifunctional conductive hydrogel, polypyrrole-polythionine-gold with glucose oxidase , was synthesised with a one-pot method using pyrrole and thionine as monomers, ammonium persulfate and HAuCl 4 as co-oxidant agents, and glucose oxidase as the doping agent. Combined with the hydrogel, a label-free amperometric immunoassay for the tumour marker neuron-specific enolase (NSE) was obtained. If operated at 0 V (vs. Ag/AgCl), the sensor exhibited a wide liner range from 100 ng mL −1 to 1 pg mL −1 and ultralow detection limit of 0.65 pg mL −1 (S/N = 3). The presented method was further used for the analysis of human serum samples and showed good consistency with detection results from an electrochemiluminescence immunoassay.
研究领域:
肿瘤学
·
免疫学
·
生物传感器
·
分子诊断
·
转化医学
·
临床检验医学
·
纳米生物技术
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Peng Wen,Yuan-Yuan Yang,Wen-Jia Yin,Jian-Cheng Hu,Yu-Dong Shen,Yu Wang,Zhen-Lin Xu,Zhi-Li Xiao,Hong-Tao Lei,Jin-Yi Yang,Hong Wang,Bruce D. Hammock
19-nortestosterone (19-NT), being regarded as the residual marker of banned synthetic steroid hormone nandrolone phenylpropionate , could cause adverse effects when consumed by human. To ensure food safety and minimize 19-NT toxicity, herein, an innovative electrochemical immunosensor for detection of 19-NT was constructed based on the modified polyvinyl alcohol/polyacrylic acid (PVA/PAA) nanofiber mat (NFM). The detection was achieved via a direct competition for binding to horse radish peroxidase-tagged anti-19-NT nanobody between free 19-NT and the 19-NT-ovalbumin covalently immobilized onto the NFM, and the amperometric response of immunosensor was analyzed using cyclic voltammetry . Under the optimal preparation conditions, the PVA/PAA NFM with appropriate hydrophilicity and ultra-high surface was obtained and characterized, and its electrochemical performance exhibited a 20-times increased response compared to the PVA/PAA casting membrane. The established immunosensor possessed high sensitivity towards 19-NT with a detection limit of 2.14 pg/mL, a 46-fold times lower compared to the traditional ic-ELISA of pure anti-19-NT nanobody. Recovery rates from the spiked animal urine samples ranged from 97.09 % to 117.32 %. Moreover, the sensor showed good reproducibility and stability over 6 weeks, suggesting that the nanofiber-based biosensor is a promising alternative for the fast and sensitive determination of 19-NT.
研究领域:
分析化学
·
食品安全
·
生物传感器
·
免疫学
·
环境与健康
·
药物残留检测
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2016
·
IF 4.712
Liyuan Wang,Jiao Shan,Feng Feng,Zhanfang Ma
A novel electrochemical redox-active nanocomposite was synthesized by a one-pot method using N,N′-diphenyl-p-phenylediamine as monomer , and HAuCl 4 and K 2 PtCl 4 as co-oxidizing agents. The as-prepared poly(N,N′-diphenyl-p-phenylediamine)-Au/Pt exhibited admirable electrochemical redox activity at 0.15 V, excellent H 2 O 2 electrocatalytic ability and favorable electron transfer ability. Based on these, the evaluation of the composite as sensing substrate for label-free electrochemical immunosensing to the sensitive detection of carbohydrate antigen 199 was described. This technique proved to be a prospective detection tool with a wide liner range from 0.001 U mL −1 to 40 U mL −1 , and a low detection limit of 2.3 × 10 −4 U mL −1 (S/N = 3). In addition, this method was used for the analysis of human serum sample, and good agreement was obtained between the values and those of enzyme-linked immunosorbent assay, implying the potential application in clinical research. Importantly, the strategy of the present substrate could be extended to other polymer-based nanocomposites such as polypyrrole derivatives or polythiophene derivatives, and this could be of great significance for the electrochemical immunoassay .
研究领域:
电分析化学
·
生物传感器与生物电子学
·
纳米复合材料
·
免疫分析
·
临床诊断
✓ 已验证
DOI
高影响因子
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
·
2016
·Vol. 44
·期 8
·1286-1290
·
IF 0.566
Li-Hua DENG,Jin-Bo DAI,Zhen-Lin XU,Jin-Yi YANG,Hong WANG,Zhi-Li XIAO,Hong-Tao LEI,Yuan-Ming SUN,Yu-Dong SHEN
To detect furaltadone metabolite 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in fish sample, a Eu 3+ labeling time-resolved fluoroimmunoassay (TRFIA) was developed. The experimental conditions were optimized as follows: the AMOZA-OVA concentration was 0.25 μg mL −1 ; the antibody was diluted by 50000 folds, and the competitive reaction time was 50 min. Under the optimal conditions, the method showed a detection limit of 0.01 ng mL −1 , an IC 50 of 0.26 ng mL −1 and a linear range (IC 20 −IC 80 ) of 0.025−2.83 ng mL −1 . The spiked recoveries of AMOZ in fish at three spiked levels ranged from 78.0% to 86.0%, and the relative standard deviations were less than 15%. Good correlation between ic-TRFIA and high performance liquid chromatography-tandem mass spectrometry was obtained for spiked food samples. The proposed ic-TRFIA method was suitable for the determination of AMOZ residue in food samples.
研究领域:
食品安全
·
分析化学
·
免疫分析
·
兽药残留
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2017
·Vol. 7
·期 1
·1-10
·
IF 4.259
Lu Wenting,Wang Kan,Xiao Kun,Qin Weijian,Hou Yafei,Xu Hao,Yan Xinyu,Chen Yanrong,Cui Daxiang,He Jinghua
A novel immunomagnetic nanobeads -based lateral flow test strip was developed for the simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA), which are sensitive and specific in the clinical diagnosis of small cell lung cancer. Using this nanoscale method, high saturation magnetization, carboxyl-modified magnetic nanobeads were successfully synthesized. To obtain the immunomagnetic probes, a covalent bioconjugation of the magnetic nanobeads with the antibody of NSE and CEA was carried out. The detection area contained test line 1 and test line 2 which captured the immune complexes sensitively and formed sandwich complexes. In this assay, cross-reactivity results were negative and both NSE and CEA were detected simultaneously with no obvious influence on each other. The magnetic signal intensity of the nitrocellulose membrane was measured by a magnetic assay reader. For quantitative analysis, the calculated limit of detection was 0.094 ng/mL for NSE and 0.045 ng/mL for CEA. One hundred thirty clinical samples were used to validate the test strip which exhibited high sensitivity and specificity. This dual lateral flow test strip not only provided an easy, rapid, simultaneous quantitative detection strategy for NSE and CEA, but may also be valuable in automated and portable diagnostic applications.
研究领域:
生物医学工程
·
临床诊断
·
纳米生物技术
·
肿瘤学
·
免疫分析
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）