Food Chemistry-X
·
2025
·
IF 8.2
Yu-Jing Huang,Jing Feng,Yong Deng,Jie Li,Hong-Yan Liu,Qing Liang,Yi-Chen Hu,Jian-Yong Zhang,Liang Zou,Ding-Tao Wu
Pectic polysaccharides are the mainly bioactive components in Lithocarpus litseifolius (sweet tea) leaves. Nevertheless, owing to their diverse and complex chemical structures, the detailed structure-function relationships (SFR) of pectic polysaccharides from sweet tea (STP) are still unclear. Herein, the influence of STP's esterified degree on its diverse biological functions was uncovered. The results showed that the de-esterified STPs with a middle-esterified degree (33.53 %) and a low-esterified degree (7.66 %) were successfully prepared when compared with the original high-esterified STP (47.01 %), and their primary structural features were almost stable after the controllable de-esterification treatment. Furthermore, the findings inferred that STP's antioxidant, anti-diabetic, and immunostimulatory functions showed inverse correlations with the esterified degree. Moreover, the de-esterified STP with a lower-esterified degree could be more easily utilized by intestinal microorganisms to positively regulate the gut microbial composition. Overall, these findings can provide valuable insights for elucidating STP's precise SFR.
研究领域:
植物化学
·
功能食品
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Jing Feng,Xiao Luo,Bo Li,Xiao-Yan Yang,Lin Zhang,Jing-Wei Huang,Yi-Chen Hu,Liang Zou,Ding-Tao Wu
Pectic polysaccharides are well-recognized as the mainly functional components in jujube fruits, which have garnered considerable attentions to be exploited into functional food products. Nevertheless, the knowledge about the influence of the producing area on their fine structural features and biological functions is still scarce, which may restrict their rational use in the functional food sector. Therefore, the structural and biological properties of pectic polysaccharides from jujube fruits (JP) collected from different producing areas were compared. The results showed that JP's physicochemical properties (e.g., total uronic acids, total bound polyphenols, molecular mass distributions, and ratios of constituent monosaccharides) varied significantly depending on the producing area. However, the primary chemical structures of JPs obtained from various producing areas were largely similar, predominantly composing of homogalacturonan and rhamnogalacturonan-I regions. Furthermore, JP's antioxidant and immunostimulatory effects also varied remarkedly depending on the producing area, primarily attributed to variations in their physicochemical properties. Particularly, JP's antioxidant activity demonstrated a significantly positive correlation with the levels of total uronic acids and total bound polyphenols, and similarly, its immunostimulatory activity was highly dependent on the level of total uronic acids. Moreover, mechanistic investigations revealed that JP could enhance immune responses via activating the Toll-like receptor 4 mediated nuclear factor-κB signaling pathway in RAW 264.7 macrophages. Collectively, the current findings could provide valuable insights for the rational application of jujube fruits and JP in the functional food sector.
研究领域:
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2022
·
IF 6.449
Dongxue Cao,Enwei Wei,Zeyu Wang,Zhengyu Hu,Ling Qi,Hongli Zhou,Jingli Zhao
This paper aimed to elaborate the chemical characteristics of ginseng polysaccharide via chemical methods and instrumental analyses; additionally, the anti-glioma activity and immunostimulatory capacity were also evaluated via biological experiments. Ginseng polysaccharide by microwave-assisted extraction (MEGP) method was purified by spongiform diethylaminoethyl cellulose (DEAE) and further separated by DEAE-52 column chromatography to obtain four MEGP fractions (MEGP-0, MEGP-1, MEGP-2, MEGP-3). Utilizing Zeta potential analyzer and X-ray photoelectron spectroscopy (XPS), MEGPs were, for the first time, confirmed the valence state of C and O with the binding mode of elements, and all MEGP fractions contained triple-helix structure. MEGP-2 and MEGP-3 significantly reduced the cell growth rate of TJ905 and U87 , showing an inhibitory effect on glioma. Among four fractions, MEGP-2 markedly elevated the immunostimulatory capacity via the activation of MAPK signaling route, with an increasing the expression levels of p-p38, p-ERK and p-JNK. In summary, this paper laid a solid foundation for the development of ginseng, as a promising drug or tonic, in the fields of anti-glioma and immunoregulation.
研究领域:
药理学
·
肿瘤学
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
Frontiers in Bioengineering and Biotechnology
·
2024
·
IF 4.3
He Wei,Zhang Xinya,Zou Yangxiaoyu,Li Ji,Chang Le,He Yu-Cai,Jin Qiuheng,Ye Jianren
Introduction: Circular RNAs (circRNAs) are endogenous noncoding RNAs (ncRNAs) with transcriptional lengths ranging from hundreds to thousands. circRNAs have attracted attention owing to their stable structure and ability to treat complicated diseases. Our objective was to create a one-step reaction for circRNA synthesis using wild-type T7 RNA polymerase as the catalyst. However, T7 RNA polymerase is thermally unstable, and we streamlined circRNA synthesis via consensus and folding free energy calculations for hotspot selection. Because of the thermal instability, the permuted intron and exon (PIE) method for circRNA synthesis is conducted via tandem catalysis with a transcription reaction at a low temperature and linear RNA precursor cyclization at a high temperature.Methods: To streamline the process, a multisite mutant T7 RNA polymerase (S430P, N433T, S633P, F849I, F880Y, and G788A) with significantly improved thermostability was constructed, and G788A was used.Results: The resulting mutant exhibited stable activity at 45°C for over an hour, enabling the implementation of a one-pot transcription and cyclization reaction. The simplified circRNA production process demonstrated an efficiency comparable to that of the conventional two-step reaction, with a cyclization rate exceeding 95% and reduced production of immunostimulatory dsRNA byproducts.
研究领域:
分子生物学
·
生物化学
·
合成生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Chemistry-X
·
2025
·
IF 8.2
Junhui Li,Shan Li,Liyan Cheng,Xiaoliang Zheng,Linglan Tu,Jinjun Li,Shiguo Chen
Hawthorn has been traditionally used in Chinese food and medicine, with pectic polysaccharides exhibiting multiple health benefits. This study focused on a homogalacturonan-enriched pectic polysaccharide (HPS) from the new Crataegus pinnatifida cultivar Jinruyi. HPS had a molecular weight of 267 kDa and was composed of rhamnose (Rha), galacturonic acid (GalA), glucose (Glc), galactose (Gal), xylose (Xyl), and arabinose (Ara), with GalA comprising 80.05 %. It was partially depolymerized during simulated digestion and highly fermentable during in vitro human fecal fermentation. Like other polysaccharides, HPS promoted short-chain fatty acid production, but, unlike neutral sugar-rich polysaccharides that mainly increase propionate and butyrate, HPS significantly enhanced acetate levels. HPS also increased beneficial bacteria such as Faecalibacterium . Additionally, HPS fermentation enhanced nitrogen monoxide (NO), reactive oxygen species (ROS) generation, phagocytic activity and cytokine generation in RAW 264.7 cells. These results suggest HPS may improve gut health by modulating gut microbiota and producing beneficial fermentation products.
研究领域:
食品科学
·
天然产物化学
·
微生物组学
·
免疫学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
·
2020
·
IF 5.085
Kong Xianghui,Duan Weiwen,Li Dingjin,Tang Xiaoxian,Duan Zhenhua
Recently, the immuno-enhancing potential of polysaccharide from Auricularia auricula (AAP) has been an area of research interest. However, the immune-stimulatory activity and mechanisms of AAP in immunosuppressive mice treated with cyclophosphamide (CTX) are still poorly understood. This study aimed to evaluate the immuno-enhancing effects of AAP and mine its possible mechanisms. Firstly, polysaccharides were isolated from A. auricula and purified. Secondly, the immune-stimulatory activities of the first AAP fraction (AAP1) were evaluated in the CTX-treated mice. Results showed that AAP1 significantly enhanced immune organ indexes, remarkably stimulated IFN-γ, IL-2, IL-4, IL-10, and TNF-α levels in the serum, and dramatically up-regulated the mRNA levels of Claudin-1, Occludin and ZO-1. Compared to the CTX group, AAP1 administration restored the gut microbiota composition similar to that of the control group by decreasing the ratio of Firmicutes/Bacteroidetes and increasing the relative abundances of short-chain fatty acid-producing microbiota. This study provides useful information for its further application as an immune-stimulator in foods and drugs.
研究领域:
免疫学
·
微生物学
·
营养科学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
Polymer Chemistry
·
2016
·Vol. 7
·期 27
·4531-4541
·
IF 5.687
Chunbao Du,Nan Zhang,Shichao Ding,Xumian Gao,Ping Guan,Xiaoling Hu
The surface functionalities of nanomaterials provide an invaluable resource to biomaterials science in the form of biomolecular recognition, sensing, self-assembly and drug delivery. This paper presents a one-step procedure for the facile preparation of highly cross-linked raspberry-like nano/microspheres by the dispersion polymerization of polyethylene glycol dimethacrylate and N-vinylimidazole. Ionic liquid-functionalized reactions were then conducted on the surface of the raspberry-like nano/microspheres to investigate the effects of the functional groups on the peptide adsorption in aqueous media. Various characterization methods were employed to characterize the as-prepared nano/microspheres and to track the experiment process. The results reveal that the nano/microspheres were raspberry-like, monodisperse and stable. It was also found that the adsorption of immunostimulating hexapeptide from human (IHH) was correlated with the surface properties of the ionic liquid-functionalized nano/microspheres, which demonstrated their potential use for the immobilization and release of IHH in practical application.
研究领域:
生物材料科学
·
药物递送
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Yang Yi,Xiao-Yun Huang,Zhao-Tian Zhong,Fei Huang,Shu-Yi Li,Li-Mei Wang,Ting Min,Hong-Xun Wang
To investigate the structure, in vitro digestibility and activity of polysaccharides from lotus root, their main fractions named LRPs were isolated and purified by gel filtration chromatography. Structural analyses indicated that: LRPs were α‑(1 → 6)‑ d ‑heteroglucans mainly composed of Glc-(1→, →6)-Glc-(1→, →6)-Gal-(1→, →4,6)-Gal-(1→ and →3,6)-Glc-(1→ at a molar ratio of 1.00: 4.33: 0.83: 0.13: 1.14; the total molar percentage of other monosaccharides in LRPs, including Man, Rha, GalA and Ara, was 8.10%; the molecular weights of LRPs was in the range of 1.33 kDa to 5.30 kDa. According to the change of molecular weight and the productions of reducing sugar and free monosaccharide, the simulated experiments of salivary, gastric and intestinal digestion confirmed that LRPs were almost undigestible. Moreover, LRPs showed the scavenging ability against DPPH and hydroxyl radicals, the growth inhibition ability against SGC7901 and HepG2 cancer cells in vitro , and the immunostimulating effect on the NO and TNF-α productions of macrophages in vitro . LRPs nearly remain their initial structure and activities in upper gastrointestinal tract and have health-improving potentials.
研究领域:
食品科学
·
营养学
·
生物化学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2015
·
IF 2.912
Qingxia Yuan,Longyan Zhao,Qianqian Cha,Yi Sun,Hong Ye,Xiaoxiong Zeng
Sinonovacula constricta has been widely used as a health food and medicine in China, Japan, and Korea. In the present study, a water-soluble polysaccharide fraction (SCP-1) was prepared from S. constricta by enzyme-assisted extraction and purification of chromatography with DEAE-52 cellulose anion-exchange column and Sephadex G-100 size exclusion column. On the basis of the analytical results of high-performance liquid chromatography, Fourier transform-infrared spectroscopy, methylation analysis, and NMR spectroscopy, SCP-1 was found to have an average molecular weight of 15.63 kDa and a linear backbone of (1→4)-linked α-d-Glcp residue with one branch, α-d-Glcp, attached to the main chain by a (1→6) glycosidic bond at every five α-d-Glcp units. Furthermore, it was found that SCP-1 could significantly increase the viability of macrophages, enhance the capability of macrophage phagocytosis, increase the activity of acid phosphatase, and promote the production of nitric oxide, mouse tumor necrosis factor (TNF)-α, mouse interferon (IFN)-γ, and mouse interleukin (IL)-1β. The results suggest that SCP-1 possesses potent immunomodulating effect and may be explored as a potential biological response modifier.
研究领域:
海洋生物学
·
免疫学
·
药理学
✓ 已验证
DOI
PubMed
高影响因子
SYNTHESIS-STUTTGART
·
2021
·Vol. 53
·期 14
·2435-2448
·
IF 3.157
Xin Zhou,Qing Long,Dongwei Li,Jingru Gao,Qikai Sun,Shaozi Sun,Yong Su,Peng Wang,Wenjie Peng,Ming Li
Structurally defined and pure oligosaccharides corresponding to β-glucans have attracted great attention because of their potential properties as immunostimulating agents and as antigens of vaccine candidates. We herein describe a convergent synthesis of ready-to-conjugate tridecasaccharides composed of a β-1,3-glucan nonasaccharide backbone and a β-1,6-glucan tetrasaccharide branch. The assembly was achieved by employing trichloroacetimidate glycosylations and features the gram-scale preparation of the nonasaccharide backbone and installation of the tetrasaccharide branch involving orthoester rearrangement to the glycoside.
研究领域:
有机化学
·
生物化学
·
疫苗研究
✓ 已验证
DOI
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Lin Xi Huang,Fang Ting Gu,Yan Yu Zhu,Zi Chen Zhao,Jun Hui Li,Jian Yong Wu
Polysaccharides from Lentinula edodes mushrooms (LePS) are well-known for their immunomodulatory activities, which may have a functional connection with probiotic fermentation in the gut microbiota. This study was to evaluate the bifidogenic properties and impact of Bifidobacterial fermentation on the LePS molecules and immunoactivities. Two LePS fractions, LePS-40 (3.97 × 10 7 Da) and LePS-80 (1.43 × 10 5 Da) with differences in molecular weight (MW) and chemical composition were isolated from the mushroom hot-water extract and fermented with a Bifidobacterium breve strain. The higher-MW fraction LePS-40 was more significantly consumed and utilized by the bacteria for growth and acetic acid production during the fermentation, due probably to its higher total carbohydrate content and glucose-abundant composition. The Bifidobacterial fermentation caused a notable MW reduction of both LePS fractions and, more interestingly, also led to a higher immunostimulatory activity in RAW 264.7 macrophage cells. Further assessment of the separated fractions by ultrafiltration of the fermentation liquid (digesta) suggested that the immunoactivity was mainly attributed to the partially degraded LePS instead of the small-molecule metabolite products derived from the Bifidobacterial fermentation. In summary, monosaccharide composition was a more significant determinant than MW on the bifidogenic properties of LePS and the immunomodulatory activities after fermentation.
研究领域:
微生物学
·
免疫学
·
食品科学
·
真菌学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Guijie Chen,Yixun Bai,Ziqi Zeng,Yujia Peng,Wangting Zhou,Wenbiao Shen,Xiaoxiong Zeng,Zhonghua Liu
Fuzhuan brick tea (FBT), one of the unique dark teas, has various health-promoting functions. In the present study, one polysaccharide fraction, namely FBTPS-2-1, was extracted and purified from FBT, and its structure and potential immunostimulatory activity were investigated. The results showed that FBTPS-2-1,one of typical heteropolysaccharides, was mainly composed of Gal, Ara, and Glc with little molar content of Man, Rha, GalA, and GlcA in molar ratio of 46.59:22.13:13.57:8.20:6.02:2.12:1.38 and molecular weight of 748 kDa. The backbone of FBTPS-2-1 contained →4)-β-d-Galp-(1→4)-β-d-Galp-(1→, →4)-β-d-Galp-(1→4)-α-d-Glcp-(1→, →4)-α-d-Glcp-(1→4)-α-d-Glcp-(1→, →4)-α-d-Glcp-(1→4)-β-d-Galp-(1→, →3)-β-d-Galp-(1→4)-β-d-Galp-(1→, →3,6)-β-d-Galp-(1→3)-β-d-Galp-(1→ and →3,6)-β-d-Galp-(1→3,6)-β-d-Galp-(1→. The linkages of branches in FBTPS-2-1 were mainly composed of α-l-Araf-(1→3,6)-β-d-Galp-(1→, →5)-α-l-Araf-(1→3,6)-β-d-Galp-(1→, →6)-β-d-Galp-(1→3,6)-β-d-Galp-(1→, α-l-Araf-(1→3,5)-α-l-Araf-(1→, →3,5)-α-l-Araf-(1→5)-α-l-Araf-(1→, α-d-Galp-(1→3,5)-α-l-Araf-(1→ and →5)-α-l-Araf-(1→6)-β-d-Galp-(1→. Furthermore, FBTPS-2-1 could increase the phagocytosis of macrophages and promote the secretion of NO and a variety of inflammatory cytokines, including TNF-α, IL-1β, and IL-6, indicating noticeable immune enhancement activity. Thus, FBTPS-2-1 could serve as a potentially functional food to improve human health by modulating the host immunoreaction.
研究领域:
食品科学
·
免疫学
·
药化化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）