Aquaculture Reports
·
2024
·
IF 3.2
Menglin Shi,Liangjuan Sun,Liutong Chen,Kangyuan Qu,Beiping Tan,Shiwei Xie
This experiment investigated the impacts of hydroxyproline (Hyp) supplementation in low fish meal diets on growth, antioxidant capacity, immunity and intestinal health of Litopenaeus vannamei . Six diets were formulated for the experiment, with the high fish meal diet containing 25 % fish meal (HF) and the remaining diets incorporating a mixture of soybean protein concentrate (SPC) and Clostridium autoethanogenum protein (CAP) replacing 15 % fish meal. These diets were supplemented with varying levels of Hyp (0 %, 0.2 %, 0.4 %, 0.6 %, and 0.8 %, designated as LF, H1, H2, H3, and H4, respectively). A total of 960 healthy shrimp (0.32±0.00 g) were divided into 6 groups at random; each group had 4 duplicates, each containing 40 shrimp. The shrimp were fed four times a day for a period of 51 days. The results indicated that dietary Hyp supplementation had no significant effects on growth performance and whole body composition of L. vannamei ( P > 0.05). The antioxidant enzyme activities of hemolymph and hepatopancreas were significantly decreased after fish meal was replaced, and were significantly increased after dietary Hyp supplementation ( P < 0.05). The expression of antioxidant-related genes in intestinal and hepatopancreas was significantly increased after dietary Hyp supplementation ( P < 0.05). In the intestine, the gene expression of penaeidins 3 ( pen3 ), dorsal , anti-lipopolysaccharide factor ( alf ) and crustins ( cru ) in the H2 group is the highest, and the gene expression of B-cell lymphoma-2 ( bcl2 ) in the H3 group is the highest ( P < 0.05). The genes expression of tumor necrosis factor-alpha ( tnf-a ), Bcl-2-assoxicated x protein ( bax ) and cytochrome c ( cyc-t ) in LF group reached the maximum ( P < 0.05). Intestinal H&E stained sections showed that low-fish meal diet reduced the mucosal folds height of shrimp, and H2 and H3 groups significantly increased the mucosal fold height of shrimp ( P < 0.05). Therefore, adding Hyp to the diet can improve the antioxidant capacity, immunity and intestinal health of L. vannamei .
研究领域:
水产养殖学
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营养学
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免疫学
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DOI
高影响因子
ACS Applied Bio Materials
·
2018
·
IF 0
Hui-Jiuan Chen,Di-an Lin,Fanmao Liu,Lingfei Zhou,Di Liu,Zhihong Lin,Chengduan Yang,Quanchang Jin,Tian Hang,Gen He,Xi Xie
Bioactive functional probiotics play an important role in many health applications such as maintaining skin health and the immunity of the human host. Artificial supplementation of probiotics would enhance immune functions as well as regulate skin health. However, simple and effective methods to deliver probiotics into the dermis to regulate local dermal tissue are still lacking. Furthermore, microneedles have been used for transdermal drug delivery in a pain-free manner, yet there were few reported methods to deliver living microbes via microneedles. In this work, we developed a technique to deliver bioactive functional probiotics, using lactobacillus as the model probiotic, into local dermis by dissolvable microneedles. The transdermal delivery of probiotics might enhance local skin regulation and immunity, and dissolvable microneedles served as a safe and pain-free tool for dermal microbial delivery. Lactobacillus was encapsulated in dissolvable microneedles with high viability by a centrifugation casting method. The microneedles rapidly dissolved after skin penetration, releasing the lactobacillus into the subcutaneous space, without causing local tissue irritation. The lactobacillus was functionally bioactive following transdermal delivery, actively synthesizing lactic acid both ex vivo and in vivo. Our technique provided a safe, effective, and convenient approach for the transdermal delivery of probiotics into local skin, with the potential to improve skin health and immunity.
研究领域:
生物医学工程
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微生物学
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药剂学
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DOI
PubMed
Aquaculture Reports
·
2023
·
IF 3.7
Yi Du,Long Cheng,Jianhua Zhao,Clement R. de Cruz,Hong Xu,Liansheng Wang,Qiyou Xu
This study investigated the effects of Clostridium butyricum (CB) and sodium butyrate (SB) on the growth performance, intestinal immune, and gut microbiota of mirror carp ( Cyprinus carpio ) fed with a high level of soybean meal. Three hundred sixty healthy carp fingerlings (∼3.74 g) were divided into eight dietary treatments with four replicates and ten fish per replicate. Which include basal diet (Control group, C0); C0 supplemented with three levels of CB, 3 × 10 7 cfu/kg (C1), 3 × 10 8 cfu/kg (C2), 3 × 10 9 cfu/kg (C3); three levels of SB, 500 mg/kg (S1), 1000 mg/kg (S2), 2 000 mg/kg (S3); and 3 × 10 8 cfu/kg CB+ 1 000 mg/kg SB (CS), respectively. In 8-week trial, the survival rate of all groups was 100%. Compared with C0, the Weight Gain, Feed Conversion Ratio, Protein Efficiency Ratio were not significantly different in all groups ( P > 0.05). Crude protein and ether extract contents were increased significantly ( P < 0.05) when supplemented with different levels of CB and SB, and ash content increased significantly in the CB groups ( P < 0.05). Serum globulin increased and glucose decreased significantly ( P < 0.05) in the CB and SB groups. The intestinal T-SOD and glutathione activity in the foregut, midgut, and hindgut were increased ( P < 0.05), and villus height of foregut and midgut were higher in the CB and SB ( P < 0.05). Protease activities in the foregut and midgut were significantly increased ( P < 0.05) in the C2, C3, S2, and S3. The lipase activity of the C2 and C3 in foregut, midgut, and hindgut; S2, S3, and CS in the foregut; S3 in midgut were significantly increased ( P < 0.05). The gut microbiota was modulated at the phylum and genera level by CB and SB administration. In summary, our results indicated that CB and SB could promote the whole body CP and EE content, intestine immunity, digestive enzyme activity, and intestinal morphology, and modulated gut flora in carp.
研究领域:
水产养殖
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营养学
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微生物学
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渔业科学
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产品关联
DOI
高影响因子
JOURNAL OF APPLIED PHYCOLOGY
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2024
·Vol. 36
·期 4
·1781-1792
·
IF 2.8
Zhu Bingkui,Sun Fengjie,Gao Zhengquan,Yao Ting,Han Subing,Zhang Hao,Ma Yuyang,Wu Yuyong,Du Ran,Meng Chunxiao,Cui Yulin
As harmless alternative of antibiotics, two antimicrobial peptides (AMPs), including anti-lipopolysaccharide factor isoform 3 (ALFPm3) and cathelicidin (CATHPb1), were co-expressed in the common fish food Tetraselmis subcordiformis. Lysates from T. subcordiformis transformant were able to inhibit the bacterial growth of Staphylococcus aureus, Vibrio parahaemolyticus, and Vibrio splendidus. To test the effects of AMPs in vivo, turbot ( Scophthalmus maximus ) underwent three dietary treatments, including commercial feeds (CON group), commercial feeds mixed with wild-type T. subcordiformis (WT group), and commercial feeds mixed with a mutant strain of T. subcordiformis (CSD group). Compared to CON group , both CSD and WT groups showed an increase in both the relative abundance and diversity of beneficial bacteria. The content of malondialdehyde was reduced and the enzymatic activities of lysozyme were increased in plasma. Comparisons of the biochemical factors in CSD group with those in CON group revealed that the relative abundance of Vibrio was decreased from 92.7% to 75.1%, and the number of bacteria containing the potentially pathogenic phenotypes was reduced. In the CSD group, the enzymatic activities of catalase and the expression levels of IgM, NKEF, IFNγ, MHC I, and IL-8R were higher compared to the WT group, whereas the levels of both TNFα and IL-1β were reduced. These results indicated that as an oral delivery vehicle for the AMPs, T. subcordiformis transformant improved the immunity, inflammatory response, and gut microbiome community of turbot.
研究领域:
海洋生物学
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免疫学
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微生物学
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水产养殖
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产品关联
DOI
高影响因子
Aquaculture Reports
·
2023
·
IF 3.7
Menglin Shi,Xinzhou Yao,Kangyuan Qu,Yucheng Liu,Beiping Tan,Shiwei Xie
This experiment investigated the impact of taurine supplementation in low fishmeal diets on growth, antioxidant capacity, immunity and intestinal health of Litopenaeus vannamei . In this experiment, five diets were formulated, of which the high fishmeal diet contained 25% fishmeal (HF) and the remaining four diets were a mixture of soybean protein concentrate and Clostridium autoethanogenum protein (CAP) replacing 15% fishmeal and supplemented with 0%, 0.2%, 0.4% and 0.6% taurine (LF, T1, T2, T3). A total of 800 healthy shrimp (0.32 ± 0.00 g) were randomly allocated into 5 groups, with each group consisting of 4 replicates and 40 shrimp per replicate. The shrimp were fed four times a day for 51 days. The results showed that there was no significant difference in weight gain rate and survival ( P ＞0.05). Dietary taurine supplementation significantly improved the antioxidant capacity of shrimp. Compared with LF group, the mRNA expression of alf (anti-lipopolysaccharide factor) and cru (crustins) in HF group were significantly up-regulated ( P ＜0.05). Compared with the HF and LF groups, the mRNA expression of pen3 (penaeidins 3) in T1, T2 and T3 groups was significantly up-regulated, and the mRNA expression of dorsal in T2 group was significantly up-regulated ( P ＜0.05). The mRNA expression of bcl-2 (B-cell lymphoma-2) in T2 group was significantly up-regulated than that in other groups ( P ＜0.05). The mRNA expression of cyt-c (cytochrome c) in LF group was significantly up-regulated than that in other groups ( P ＜0.05). Compared with LF group, the mRNA expression of tnf-a (tumor necrosis factor-alpha) in HF, T2 and T3 groups was significantly down-regulated ( P ＜0.05). The mRNA expression of caspase8 in HF and T2 group was significantly down-regulated than that in other groups ( P ＜0.05). Compared with LF group, the mRNA expression of caspase3 in T1 groups was significantly down-regulated ( P ＜0.05). Intestinal H&E stained sections showed that dietary taurine supplementation improved intestinal mucosal folds height, width and muscle layer thickness. In general, dietary taurine supplementation can improve the antioxidant capacity, immunity and intestinal health of Litopenaeus vannamei , and the optimal supplemental level is 0.4%.
研究领域:
水产养殖
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营养学
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免疫学
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海洋生物学
✓ 已验证
DOI
高影响因子
Toxins
·
2023
·Vol. 15
·期 8
·478
·
IF 4.2
Mubashar Hassan,Yanan Wang,Shahid Ali Rajput,Aftab Shaukat,Ping Yang,Muhammad Zahid Farooq,Qianhui Cheng,Mehboob Ali,Xiaomei Mi,Yu An,Desheng Qi
Deoxynivalenol (DON, Vomitoxin) is a threatening mycotoxin that mainly produces oxidative stress and leads to hepatotoxicity in poultry. Antioxidant dietary supplements dramatically boost immunity, safeguarding animals from DON poisoning. Luteolin (LUT) is an active plant-derived compound that poses influential antioxidants. This study explored the effectiveness of LUT in combination with activated charcoal (AC) in detoxifying DON in broilers. The 180 one-day broiler chickens were allocated into five different groups having six replicates in each group, provided with ad libitum feed during the trial period (28 days) as follows: in the control group, basal diet (feed with no supplementation of LUT, AC or DON); in group 2, a basal diet added with 10 mg/kg DON from contaminated culture (DON); in group 3, a basal diet augmented by 350 mg/kg LUT and DON 10 mg/kg (DON + LUT); in group 4, a basal diet supplemented by DON 10 mg/kg + AC 200 mg/kg (DON + AC); and in group 5, a basal diet supplemented by 10 mg/kg DON + 350 mg/kg LUT + 200 mg/kg AC (DON + LUT + AC). Concerning the control group, the DON-treated broilers demonstrated a significant decrease in growth performance (p< 0.05) and serum immunoglobulin (p< 0.05) contents, negatively changing the serum biochemical contents and enzymatic activities and an increase in histopathological liver lesions. Furthermore, DON substantially increased (p< 0.05) malondialdehyde (MDA) concentration and decreased total superoxide dismutase (T-SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) levels in the serum and liver. The intake of AC and LUT to the DON-contaminated diet decreased DON residue in the liver and potentially reduced the adverse effects of DON. Considering the results, supplementation of LUT with mycotoxin adsorbent has protective effects against mycotoxicosis caused by DON. It could be helpful for the development of novel treatments to combat liver diseases in poultry birds. Our findings may provide important information for applying LUT and AC in poultry production.Keywords:deoxynivalenol;luteolin;activated charcoal;growth performance;antioxidant capacity;immunity;broilersKey Contributions:Supplementation of luteolin and activated charcoal for four weeks improve the immune system and antioxidant capacity and protects liver injury in broiler chickens against DON-induced toxicity.
研究领域:
家禽科学
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毒理学
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营养学
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免疫学
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抗氧化研究
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产品关联
DOI
PubMed
高影响因子
FISH & SHELLFISH IMMUNOLOGY
·
2022
·
IF 4.622
Yipeng Gu,Kai Chen,Bingwen Xi,Jun Xie,Xuwen Bing
Paeonol, a naturally occurring polyphenol isolated from medical plant , has been known to exhibit anti-oxidative and anti-inflammatory effects. In order to evaluate the effect of paeonol on Carassius auratus gibelio infected by pathogenic bacteria Aeromonas hydriphila . 750 fish were randomly divided into 5 groups, which separately treated with 0.85% sterile saline (blank), A. hydriphila (negative control), A. hydriphila with paeonol (4 mg/kg, 64 mg/kg), and A. hydriphila with enrofloxacin (12 mg/kg, positive control). Fish were anaesthetized with MS-222 (100 mg/L), and samples were collected at 6 and 72 h after A. hydriphila challenge. The results showed that compared with the negative group, the survival in paeonol groups marked increased by 14.75% and 18.94%. The plasma immunoglobulin M (IgM) was notably increased, and low density lipoprotein (LDL) was significantly decreased in paeonol groups at 6 h ( P < 0.05). The antioxidative enzymes catalase (CAT), total antioxidant capacity (T-AOC) and glutathione peroxidase (GSH-Px) were significantly increased in paeonol groups at 6 h, while malondialdehyde (MDA) and myeloperoxidase (MPO) contents were lower ( P < 0.05). The inflammatory related genes MyD88 and TLR-5 were significantly downregulated, and the TLR-3 was significantly increased in paeonol groups at 72 h ( P < 0.05). In addition, histopathological analyses showed that the lesion in liver, spleen and caudal kidney were considerably attenuated in paeonol groups. In conclusion, paeonol could increase the survival rate, mitigate oxidative damage, inflammation, tissue lesions, and improve the immunity of gibel carp challenged with A. hydrophila .
研究领域:
水产养殖
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免疫学
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微生物学
·
药理学
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产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2020
·
IF 2.87
Fan Luo,Wenxiong Wang,Meiquan Chen,Zhanjia Zheng,Dandan Zeng,Murtaza Hasan,Zhihuan Fu,Xugang Shu
Immunogenic, methionine copper-induced response had proven to be precedent in providing resistance against certain diseases in fish. This study allocates the fitness strategy for Oreochromis niloticus by introducing and incorporating the well-designed, stabilized, and biocompatible N-carbamoyl-methionine copper (NCM-Cu) as a Cu potent source in diet that enhances the bioavailability and fitness. The synchronized NCM-Cu complex was characterized by directing ultraviolet and visible spectrophotometry (UV–vis), Fourier-transform infrared (FTIR), X-ray diffractometry (XRD), thermogravimetric analysis (TGA), and single-crystal X-ray diffraction. Results revealed blue columnar crystalline, NCM-Cu complex with an empirical formula as C12H30CuN4O10S2. Anonymously, the overall growth performance of the fish remained unaltered with NCM-Cu adjunct feed. NCM-Cu significantly raised the Cu accumulation in the fish muscles, liver, gill, and intestine in contrast to the basic Cu-rich feed. The serum antioxidant enzyme activity elevated up to (ceruloplasmin: 19.38 U/L) and the lowest liver malondialdehyde (MDA) content (8.81 nmol/mg prot.) and triglyceride content (0.39 nmol/g prot.) were observed in the NCM-Cu group as compared to the basic Cu and CuSO4 groups, suggesting that NCM-Cu promoted antioxidative responses and alleviated lipid peroxidation of O. niloticus. Overweening, the synthesized complex, NCM-Cu significantly regulated the expression levels of lysozyme, immunoglobulin M, complement 4, and complement 3 up to 10.93 U/mL, 0.72, 0.77, and 1.18 mg/mL in serum, respectively. Thus, such endorsed results reveal the preeminence of NCM-Cu-supplemented diet for the fitness in O. niloticus.
研究领域:
水产养殖
·
鱼类营养学
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生物化学
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免疫学
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产品关联
DOI
PubMed
高影响因子
Heliyon
·
2023
·
IF 4
Ran Tu,Cheng Zhou,Wenfeng Huang,Zhengping Feng,Qiufang Zhao,Xiaofei Shi,Langjun Cui,Keke Chen
Rationale and objectives Fuzi, the dried root of Aconitum carmichaelii Debx, is one of the widely used traditional Chinese medicines. Fuzi polysaccharides are considered the most bioactive compounds with immunomodulatory functions, however, the mechanisms have not been evaluated. This study aims to systematically investigate the effects of Fuzi polysaccharides on the gut microbiota and immune function using a mouse model immunosuppressed with cyclophosphamide. Methods The short-chain fatty acid levels in cecal contents were measured by gas chromatography-mass spectrometry. The gut microbiota 16S rRNA gene were sequenced by next generation sequencing. The mRNA expression levels of NF-κB, IL-6, TNF-α, iNOS and COX-2 were measured using quantitative real-time polymerase chain reaction. The protein expression of occludin and zonula occludens-1 were analyzed by Western blot. The white blood cells were counted using automated hematology analyzer, and CD4 + FOXP3 + /CD4 + ratio was measured by flow cytometry. Results and Conclusions Fuzi polysaccharides had the function of elevating the concentration of acetic acid, propionic acid, isobutyric acid, and n-butyric acid in the cecum. Meanwhile, Fuzi polysaccharides could decrease the relative abundance of Helicobacter , Anaerotruncus , Faecalibacterium , Lachnospira , Erysipelotrichaceae _UCG-003, Mucispirillum , and Mycoplasma , and increase the relative abundance of Rhodospirillales , Ruminococcaceae _UCG-013, Mollicutes _RF39, Ruminococcus _1, Christensenellaceae _R-7_group, and Muribaculaceae in the gut. Furthermore, Fuzi polysaccharides exhibited the function of increasing spleen and thymus indices and number of white blood cells and lymphocytes. Fuzi polysaccharides could reverse the decreased mRNA expression of NF-кB, IL-6, and iNOS, differentiation of CD4 + FOXP3 + regulatory T cells as well as protein expression of occludin and zonula occludens-1 induced by cyclophosphamide. In addition, the mRNA and protein expression of cytokines were significantly correlated with the abundance of gut microbiota under Fuzi polysaccharides treatment. Collectively, the above results demonstrated that Fuzi polysaccharides could regulate inflammatory cytokines and gut microbiota composition of immunosuppressive mice to improve immunity, thereby shedding light on revealing the molecular mechanism of polysaccharides of traditional Chinese medicines in the future.
研究领域:
免疫学
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微生物学
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药理学
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中药学
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DOI
PubMed
高影响因子
Biomaterials Science
·
2020
·Vol. 9
·期 3
·973-984
·
IF 6.183
Ya Wen,Yiqiong Liu,Fangfang Guo,Yi Han,Qiansai Qiu,Yan Li,Haiqing Dong,Tianbin Ren,Yongyong Li
It has been suggested that immunogenic cell death (ICD) has therapeutic potential; however, its anticancer immunity is considerably hampered by the in situ immunosuppressive microenvironment within the tumor area, such as the dysfunction of antigen-presenting cells. Herein, we present an in vitro ICD-inducing modality to circumvent such impairment of immune activation. To this end, a “hot”, i.e., immunogenic, whole tumor cell vaccine is generated in vitro and subcutaneously vaccinated in the normal tissue, departing from the site of the in situ immunosuppressive tumor area, to fully leverage the ICD-inducing antitumor immunity. In particular, the immunogenic dying tumor cells, caged by cellular disulfide–thiol exchange, are mediated by photoactivation. After subcutaneous vaccination, the photoactivated caged live cell vaccine (CLCV) exerts multi-durable immunostimulatory property, which, when adjuvanted by CpG, efficiently promotes dendritic cell (DC) activation and elicits robust CD8+ T-cell responses in vivo. Importantly, the generated T-cell responses are shown to protect 75% mice preimmunized with CLCV against tumor initiation and significantly retards tumor growth in the therapeutic setting. The strategy presented here may help to enrich the current vaccine design for cancer immunotherapy.
研究领域:
癌症免疫治疗
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免疫学
·
肿瘤学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）