PHYTOMEDICINE
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2022
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IF 6.656
Zhaoming Zhu,Quanfu Zeng,Zhuxian Wang,Yaqi Xue,Tingting Chen,Yi Hu,Yuan Wang,Yufan Wu,Qun Shen,Cuiping Jiang,Chunyan Shen,Li Liu,Hongxia Zhu,Qiang Liu
Background Acne has become one of the most prevalent skin disorders , affecting mostly young people's physical and mental health globally. Cryptotanshinone (CPT) is a potential drug for acne, but its mechanism of acne treatment has not been thoroughly studied on the microbiota . Till date, only a few studies are directed to the impact of acne therapy on skin microbiota and lipid metabolites. Purpose The action mechanism of CPT treatment of acne was investigated by the strategy of microbiome integration with lipidomics . Methods The 16Sr DNA sequencing was used to detect skin microbiota composition, and absolute quantitative lipidomics was utilized to identify lipid metabolites profiles levels. Four key proteins of the glycolysis pathway were detected with the immunochemistry method. Antibacterial analysis was used to evaluate CPT treatment of acne. Results CPT significantly inhibited Staphylococcus epidermidis and Staphylococcus aureus . Combination of the skin microbiome and lipidomics analysis, 29 types of differentially expressed flora (DEFs) and 782 differentially expressed lipid metabolites (DELMs) were significantly altered, especially Staphylococcus, Corynebacterium , Ralstonia , Enhydrobacter, Burkholderia , and Streptococcus . Cer was mainly regulated by Staphylococcus and Corynebacterium , whereas TG and DG were mainly regulated by Ralstonia, Enhydrobacter, Burkholderia , and Streptococcus . The glycolysis pathway was significantly regulated by Staphylococcus on CPT treatment of acne. The energy metabolism, lipid metabolism , immune system, glycan biosynthesis , and metabolism could be reversed by CPT. Conclusion CPT might help acne rats rebuild their skin microbiota and alter lipid metabolism signatures. Furthermore, since skin microbes and skin lipid metabolites have a close correlation and are both regulated by CPT, the findings potentially provide a research foundation for the discovery of biomarkers of skin microbiome imbalance and targeted treatment of acne development mechanisms.
研究领域:
皮肤科
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微生物学
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药理学
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代谢组学
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产品关联
DOI
PubMed
高影响因子
Frontiers in Immunology
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2023
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IF 7.3
Han Xiangning,Lin Hong,Chen Xiangfeng,Wang Luefeng,Zhang Ziang,Wei Xiaojing,Sun Xun,Xie Hanyi,Pavase Tushar Ramesh,Cao Limin,Sui Jianxin
For a long time, people have suffered from uncertainty, complexity, and a low success rate in generating and screening antibodies against small molecules, which have become the core bottlenecks of immunochemistry. Here, the influence of antigen preparation on antibody generation was investigated at both molecular and submolecular levels. Neoepitopes (amide-containing neoepitopes) formed in the preparation of complete antigens are one of the most important factors limiting the efficiency of generating hapten-specific antibodies, which was verified by different haptens, carrier proteins, and conjugation conditions. Amide-containing neoepitopes present electron-dense structural components on the surface of prepared complete antigens and, therefore, induce the generation of the corresponding antibody with much higher efficiency than target hapten. Crosslinkers should be carefully selected and not overdosed. According to these results, some misconceptions in the conventional anti-hapten antibody production were clarified and corrected. By controlling the content of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) during the synthesis of immunogen to limit the formation of amide-containing neoepitopes, the efficiency of hapten-specific antibody generation could be significantly improved, which verified the correctness of the conclusion and provided an efficient strategy for antibody preparation. The result of the work is of scientific significance in the preparation of high-quality antibodies against small molecules.
研究领域:
免疫学
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生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
AQUACULTURE
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2022
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IF 5.135
Lukuan Li,Yu Wang,Zhen Zhang,Chunfang Wang
The novel proteins, i.e., Clostridium autoethanogenum (CAP), Tenebrio molitor (TM), Chlorella vulgaris (ChM), and cottonseed protein concentrate (CSM), are promising food for the future aquaculture due to the limited fishmeal (FM) resources. However, in-depth investigations on the intestinal barriers of fish when fed novel proteins are sparse, and the association between intestinal flora and physiological biomarkers is still unknown. Five isonitrogenous and isolipidic diets comprising various proteins were developed, with fishmeal serving as the control and novel proteins as the experimental diets. Largemouth bass ( Micropterus salmoides ) with an initial body weight of 211.9 ± 1.2 g were used as experimental model and fed with these five diets for eight weeks. The findings from the current study indicated that the intestinal histological structure as well as immunochemistry barrier performance of the ChM, CAP, and TM groups were superior to those of the CSM and FM groups, despite the fact that the villi height of FM was the greatest of all groups. Similarly, the intestinal inflammatory response and the permeability were elevated in CSM while decreased in ChM, CAP, and TM group. Concerning the intestinal short fatty acids (SCFAs), the levels in the CAP as well as ChM group were greater than the other proteins. From the data analysis of 16S rRNA gene sequencing, the abundance of opportunistic pathogens including (Ralstonia , Acinetobacter , Aeromonas , and Protebacteria) were higher in CSM group while attenuated in ChM, CAP and TM group. In addition, the abundance of nine types of intestinal-barriers-repairing probiotics were elevated in all novel proteins compared with the fishmeal group. These altered microbiota were highly correlated with intestinal inflammatory response, antioxidant status, immunochemistry barrier, physical barrier and SCFAs indexes by Mantel test and Pearson's correlation analysis. Collectively, the ranking of the beneficial functions among these five proteins was ChM > CAP>TM > FM > CSM according to Z -score and this study provides in-depth data manifesting the effects of novel proteins on the gut health status of large-size largemouth bass.
研究领域:
水产养殖
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营养学
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微生物学
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免疫学
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生物信息学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）