Foods
·
2022
·Vol. 12
·期 1
·52
·
IF 5.561
Jingjing Tai,Dan Qiao,Xue Huang,Huang Hu,Wanzheng Li,Xinle Liang,Fuming Zhang,Yanbin Lu,Hong Zhang
This study was aimed to investigate the structural and immunological properties of parvalbumin from mandarin fish during the Maillard reaction. The microwave-assisted the Maillard reaction was optimized by orthogonal designed experiments. The results showed that the type of sugar and heating time had a significant effect on the Maillard reaction (p< 0.05). The SDS-PAGE analysis displayed that the molecular weight of parvalbumin in mandarin fish changed after being glycated with the Maillard reaction. The glycated parvalbumin was analyzed by Nano-LC-MS/MS and eleven glycation sites as well as five glycation groups were identified. By using the indirect competitive ELISA method, it was found that microwave heating gave a higher desensitization ability of mandarin fish parvalbumin than induction cooker did. In vitro gastric digestion experiments showed that microwave-heated parvalbumin was proved to be digested more easily than that cooked by induction cookers. The microwave-assisted Maillard reaction modified the structure of parvalbumin and reduced the immunoreactivity of parvalbumin of mandarin fish.Keywords:parvalbumin;Maillard reaction;glycation site;structural and immunological properties;in vitro
研究领域:
食品科学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2020
·
IF 6.785
Ruru Chen,Mingzhou Hu,Hailing Zheng,Hui Yang,Lian Zhou,Yang Zhou,Zhiqin Peng,Zhiwen Hu,Bing Wang
The process and mechanism of silk degradation is still a bewildering mystery in the investigation and conservation of cultural relics, which rely on the development of accurate and tailored analysis technologies. Here, two advanced approaches, proteomics and immunology, were developed for determining the deterioration behavior of historic silk fabrics and artificially aged samples from the molecular to the holistic level. The surface morphology and secondary structure of silk were destroyed during degradation. Subsequently, the proteomics and immunology analysis demonstrated a new degradation model differing from previous reports. First, the amorphous region and the looser crystalline regions were destroyed together, and the macromolecular chains were broken randomly. Then, the tight β-sheet blocks in the crystalline region were exposed and deteriorated, which expedited the degradation of tight β-sheet blocks and relatively loose blocks in the crystalline domain as well as the amorphous domain, ultimately yielding small molecule polypeptides. However, the deterioration process of ancient fabrics could be accelerated by poor burial conditions, thus showing distinct destructive characteristics. Overall, the results gave us a more comprehensive and profound understanding of the degradation process of ancient silk.
研究领域:
文化遗产科学
·
蛋白质组学
·
免疫学
·
材料科学
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DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2019
·
IF 4.784
Xiaoyi Chen,Rui Tang,Tingting Liu,Wei Dai,Qian Liu,Guiping Gong,Shuang Song,Minghua Hu,Linjuan Huang,Zhongfu Wang
Schisandra sphenanthera and Schisandra chinensis are widely consumed either as food or for medicinal purposes. Nevertheless, no detailed comparative assessments of their physicochemical properties and biological activity have been reported. In this paper, using hot-water extraction, alcohol precipitation, and deproteinization, we obtained polysaccharidic extracts from Schisandra sphenanthera and Schisandra chinensis (denoted as SSP and SCP, respectively) and investigated their antioxidant and immunological activities. The extracts were different from each other with regard to sugar, protein, and uronic acid contents. Both extracts were mainly composed of arabinose, glucose, and galactose, but their contents varied greatly; SSP had more galacturonic acid. Compared with SCP, SSP had stronger free radical scavenging ability, protective effects on biomolecules, cellular antioxidant activity, owing to its higher protein (35.35 ± 1.73%) and uronic acid (12.81 ± 1.15%) contents. With respect to cell viability, neutral red phagocytosis, NO production, and acid phosphatase activity, SCP had stronger effects than SSP; this was largely due to its high levels of mannose, galactose, arabinose, and glucose. These results provide evidence to support the use Schisandra -derived polysaccharides for several purposes, including clinical, agricultural, and industrial applications.
研究领域:
药理学
·
生物化学
·
免疫学
✓ 已验证
DOI
PubMed
高影响因子
Cell Reports Physical Science
·
2023
·
IF 8.9
Qinan Wang,Sixuan Duan,Jiahao Qin,Yi Sun,Shihang Wei,Pengfei Song,Wen Liu,Jiangmin Gu,Li Yang,Xin Tu,Hao Gao,Chun Zhao
Dynamic deep learning is considered to simulate the nonlinear memory process of the human brain during long-term potentiation and long-term depression. Here, we propose a photoelectrically modulated synaptic transistor based on MXenes that adjusts the nonlinearity and asymmetry by mixing controllable pulses. According to the advantage of residual deep learning, the rule of dynamic learning is thus elaborately developed to improve the accuracy of a highly homologous database (colorimetric enzyme-linked immunosorbent assay [c-ELISA]) from 80.9% to 87.2% and realize the fast convergence. Besides, mixed stimulation also remarkably shortens the iterative update time to 11.6 s as a result of the photoelectric effect accelerating the relaxation of ion migration. Finally, we extend the dynamic learning strategy to long short-term memory (LSTM) and standard datasets (Cifar10 and Cifar100), which well proves the strong robustness of dynamic learning. This work paves the way toward potential synaptic bionic retina for computer-aided detection in immunology.
研究领域:
神经网络
·
生物医学工程
·
材料科学
✓ 已验证
DOI
高影响因子
Journal of Inflammation Research
·
2025
·
IF 4.1
Shiwen Zhang,Changqiang Wang,Jing Jiang,Linlu Li,Jiaqi Luo,Jingying Zhu,Renxu Chen
Background Immune escape of tumor cells is a common problem with tumor photothermal therapy utilizing gold nanorods (Au NRs). Whether CpG ODN, an immune adjuvant, can synergize with Au NRs to activate the immune response and its potential mechanism is not clear.Methods The effect of Au NRs combined with CpG ODN (Au NRs-C) on the activity of various immune-related cells, such as double-positive T cells, macrophages, NK cells, Th17, and Treg. The expression levels of various immune and inflammation-related factors, such as IL-1R1, IL-6, IL-17, and TNF-α were characterized. Transcriptome sequencing analysis was used to explore the potential immunomodulatory mechanisms of Au NRs-C. Whether immune activation was enhanced by antibody-functionalized Au NR upon binding to CpG ODN was assessed.Results Flow cytometry and ELISA analyses indicate that both Au NRs and CpG ODN increase pro-inflammatory cytokine levels and immune activation. However, Au NRs-C demonstrated superior immune activation potential. Furthermore, Au NRs stimulate the expression of Treg, while Au NRs-C significantly inhibit this effect. This suggests that the conjugation of CpG ODN with Au NRs not only greatly enhances the immune activation but also compensates for some of their deficiencies in eliciting immune responses. Transcriptome sequencing uncovered DEGs mainly localized to immune and pro-inflammatory cytokine pathways. PPI analysis identified six hub genes: FOXM1, HMOX1, UBE2C, E2F1, PECAM1, and FCGR3A. Moreover, CpG conjugation with antibody functionalization- Au NRs enhances immune stimulation.Conclusion Au NRs-C promotes immune activation by eliciting changes in the activity of immune-associated cells and expression of inflammatory factors through multiple pathways, such as MHC antigen presentation and Toll-like receptor-mediated immune processes.
研究领域:
免疫学
·
纳米医学
·
癌症生物学
·
生物信息学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yue Tao,Huaxin Li,Jiaheng Xie,Rui Ding,Zhiwei Zhuang,Xu Yang,Yang Zhu,Jie Shan,Xu-lin Chen
Burn-induced hypertrophic scarring is driven by persistent inflammation and fibroblast activation, yet current biomaterial strategies rarely address both components with mechanistic precision. Here, we report a microneedle-integrated coordination nanoplatform (GA-Sorafenib@MN) that simultaneously disrupts immune-mediated chemotaxis and fibrotic signaling to promote scarless regeneration of burn wounds. The design was guided by single-cell transcriptomic analysis of keloid tissue, which identified TGF-b1 as a fibroblast-specific fibrotic driver, and Sorafenib as the top therapeutic candidate via high-throughput virtual screening. To enhance solubility and anti-inflammatory performance, Sorafenib was coordinated with gallium (III), forming GA–Sorafenib nanoparticles subsequently embedded into photo-crosslinked HAMA microneedles. Mechanistically, GA-Sorafenib@MN suppresses CXCL2/CXCR2-driven macrophage chemotaxis and TGF-b/Smad3-mediated fibroblast activation, while inhibiting NLRP3 inflammasome and TLR4/NF-kB signaling. This reprograms the immune–fibrotic axis at the wound site, mitigates collagen I/III deposition, and restores ECM integrity. Our strategy establishes a transcriptomics-guided material design paradigm, enabling spatiotemporal modulation of structural immunity and offering a highly translatable solution for scarless healing of non-infectious burn wounds.
研究领域:
再生医学
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免疫学
·
纳米医学
·
生物材料
·
皮肤病学
·
转化研究
·
系统生物学
✓ 已验证
产品关联
DOI
高影响因子
Advanced Science
·
2025
·
IF 14.1
Hongyan Yu,Jingxu Zhang,Qingping Liu,Duxing Li,Ruonan Pan,Gan Miao,Yidi Chen,Zhe Kou,Guangbo Qu,Rong Zhang,Xiaoting Jin,Yuxin Zheng
Volatile organic compounds (VOCs) emitted from interior decorations are suggested to pose substantial respiratory hazards as they disturb the immune response in the lung. Despite this, the characteristics and mechanisms underlying immune cell responses to real-world VOC mixtures, rather than previously individual or dominant VOC species, remain poorly understood. Employing a whole-body inhalation exposure model, we reveal that interior decorative VOCs alter pulmonary immune cell profiles with a persistent increase in alveolar T cells. This sustained elevation results from intensified lymphoid-biased hematopoiesis in the bone marrow (BM) rather than in situ lung, which is followed by thymic maturation and subsequent recruitment to lung tissues. The ex vivo biosensor and antibody neutralization assays clarify that VOCs-induced lymphoid-biased hematopoiesis is primarily driven by osteogenic differentiation within the BM niche, a process further regulated by interleukin-6 (IL-6) and interleukin-17A (IL-17A). Moreover, the cohort study associates VOCs-expanded lymphocytes with increased risks of obstructive lung diseases, where exposed individuals show elevated IL-6 and IL-17A levels, correlating with VOC concentrations and lymphocyte proportions. These findings highlight compelling indicators (i.e., T cells) and potential interventions (i.e., IL-6 and IL-17A) for evaluating and mitigating VOC-associated respiratory risks.
研究领域:
免疫学
·
环境健康
·
呼吸医学
·
毒理学
·
造血生物学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ARCHIV DER PHARMAZIE
·
2025
·Vol. 358
·期 11
·e70121
·
IF 3.6
Ling Jiang,Jiangming Zhong,Huixiong Chen,Yi Li,Qi Zhou,Miaohong Yang,Li Lin,Peng Shu,Zhiyun Du
Retinoic acid receptor-related orphan receptor gamma-t (RORγt) is one of the nuclear receptor transcription factors that plays a key role in the differentiation of pro-inflammatory IL-17 + T helper cells. Recently, RORγt has attracted increasing attention as a potential drug target for the treatment of several human inflammatory diseases, including psoriasis and Crohn's disease. In this study, we revealed that ergosterol (ERG) was a novel RORγt inverse agonist, which decreased RORγt transcriptional activity, to regulate collagen fiber deposition and to inhibit pro-inflammatory cytokines, including TNF-α, IL-1β, IL-6, IL-17, IL-22, and IL-23 in the skin lesions of IMQ-induced mice with psoriasis. Furthermore, ERG could repair the epidermal barrier function by upregulating tight junction proteins, such as claudin, occludin, and ZO-1, thereby alleviating psoriasis symptoms. These findings suggested that ERG might be used as a promising novel RORγt inverse agonist in the management of psoriasis.
研究领域:
免疫学
·
皮肤病学
·
分子药理学
·
炎症性疾病
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2025
·
IF 3.8
Gao Hongfan,Zhang Shujing,Li Xin
Biotinylated antibodies are widely used in ELISA. However, their preparation typically requires labor-intensive purification steps to remove the excess biotinylating reagent. Here, we present a novel one-pot, purification-free strategy for preparing biotinylated antibodies, enabling a robust sandwich ELISA platform for the detection of the cardiac troponin I-C complex, a key biomarker for acute myocardial infarction. By introducing glycine (Gly) as a quenching agent to inactivate the residual biotinylating reagent (NHS-biotin esters) during the labeling process, our method eliminates the need for post-labeling purification while effectively reducing nonspecific background. Systematic optimization of labeling conditions yielded an assay with a detection limit of 1.08 pM and a broad linear quantification range from 3.6 pM to 1 nM ( R 2 = 0.99). The platform demonstrated high specificity, with recovery rates ranging from 91.5 to 101.8% and intra-assay variation below 2.9%. Furthermore, successful validation in a serum amyloid A detection model confirmed the generalizability of the Gly-mediated background suppression strategy. This cost-effective, streamlined ELISA workflow offers a powerful tool for rapid and reliable clinical diagnostics, with potential for broad application in biomarker detection. Graphical abstract This schematic contrasts the conventional and novel one-pot strategies for antibody biotinylation in ELISA. The traditional method requires a cumbersome purification step to remove excess biotin-NHS reagent after labeling. In contrast, our one-pot approach introduces a key innovation: an in-solution quenching reaction. The addition of a small molecule (e.g., glycine) efficiently inactivates residual biotin esters, allowing the biotinylated antibody to be used directly in subsequent steps, thereby streamlining the workflow.
研究领域:
免疫学
·
临床诊断
·
生物偶联化学
·
生物标志物检测
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2025
·Vol. 15
·期 11
·747
·
IF 5.6
Qianyu Yang,Zhiwei Liu,Xinrui Xu,Zihao Zhao,Ze Fan,Bin Du,Jianjie Xu,Jiwei Xu,Jiang Wang,Bing Liu,Xihui Mu,Zhaoyang Tong
Over the past few decades, Fc fragment-conjugated proteins, such as Protein A, have been extensively utilized across a range of applications, including antibody purification, site-specific immobilization of antibodies, and the development of biosensing platforms. In this study, building upon our group prior research, we designed and screened an affinity DNA functional ligand (A-DNAFL) and experimentally validated its binding affinity (KD= 6.59 × 10−8) toward mouse IgG antibodies, whose binding performance was comparable to that of protein A. Systematic evaluations were performed to assess the binding efficiency under varying pH levels and ionic strength conditions. Optimal antibody immobilization was achieved in PBST-B buffer under physiological pH 7.2–7.4 and containing approximately 154 mM Na+and 4 mM K+. Two competitive binding assays confirmed that the A-DNAFL binds to the Fc fragment of murine IgG antibody. Furthermore, molecular docking simulations were employed to investigate the interaction mode, revealing key residues involved in binding as well as the contributions of hydrogen bonding and hydrophobic interactions to complex stabilization. Leveraging these insights, A-DNAFL was utilized as a tool for oriented immobilization of antibodies on the sensing interface, enabling the construction of an immunosensor for ricin detection. Following optimization of immobilization parameters, the biosensor exhibited a detection limit of 30.5 ng/mL with the linear regression equation is lg(Response) = 0.329 lg(Cricin) − 2.027 (N= 9,R= 0.938,p< 0.001)—representing a 64-fold improvement compared to conventional protein A-based methods. The system demonstrated robust resistance to nonspecific interference. Sensing interface reusability was also evaluated, showing only 8.55% signal reduction after two regeneration cycles, indicating that glycine effectively elutes bound antibodies while preserving sensor activity. In summary, the A-DNAFL presented in this study represents a novel antibody-directed immobilization material that serves as a promising alternative to protein A. It offers several advantages, including high modifiability, low production cost, and a relatively small molecular weight. These features collectively contribute to its broad application potential in biosensing, antibody purification, and other areas of life science research.
研究领域:
生物传感
·
免疫学
·
分子识别
·
分析化学
·
核酸工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2025
·
IF 5.4
Yaqi Liu,Junhui Wang,Leyi Wang,Jialing Cao,Shiqi Lu,Pengfei Li,Yujie Wang,Jingmin Wang,Kangliang Sheng,Yongzhong Wang
Lactiplantibacillus plantarum (L. plantarum) offers probiotic benefits like inhibiting harmful bacteria, balancing gut microbiota, regulating immunity, and providing anti-inflammatory and antioxidant effects. This study explored its potential in food preservation and inflammation treatment, using homemade kimchi (Anhui, China) to isolate the bacteria. Twelve L. plantarum strains were identified through 16S rDNA sequencing, labeled HMPM2111 to HMPM2122. L. plantarum HMPM2111 was chosen for its excellent growth, acid production, tolerance, and antibacterial properties. Its anti-inflammatory, antioxidant, and antibacterial effects, particularly against Citrobacter rodentium-induced infectious colitis and extending juice storage, were further evaluated. The HMPM2111 strain shows a 78.4% survival rate in simulated gastric juice. It effectively inhibits Enterobacter cloacae and Staphylococcus aureus, with rates of 87.5% and 78.6%, and also inhibits Micrococcus luteus, Pseudomonas aeruginosa, Salmonella typhimurium, and Escherichia coli by over 50%, where Escherichia coli and Staphylococcus aureus are often considered as the causes of juice spoilage. It has strong anti-inflammatory and antioxidant properties, reducing colitis severity in a murine colitis model. These results shows that L. plantarum HMPM2111 has strong antibacterial and anti-inflammatory properties, along with good growth traits, making it a promising option for antibacterial preservation in fermented foods and reducing inflammatory biological functions.
研究领域:
食品微生物学
·
益生菌与功能食品
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免疫学
·
微生物发酵
·
炎症研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Bioscience
·
2025
·
IF 5.9
Wang Guo,Bo Li,Mao-Qing Wang,Yang Cao,Qin Yuan,Wen Liu,Yi-Chen Hu,Liang Zou,Shengpeng Wang,Ding-Tao Wu
Quinoa microgreen, as a nutrient-dense and health-promoting vegetable, contains abundant rhamnogalacturonan-I enriched pectic polysaccharides. However, their protective effects against ulcerative colitis (UC) and the influence of structural features like degree of esterification (DE) are poorly understood. Consequently, to overcome this gap in understanding, we systematically assessed the anti-UC activity of quinoa microgreen pectic polysaccharides (QMP) exhibiting distinct DE levels: QMP-HDE (28.49 %), QMP-MDE (16.8 %), and QMP-LDE (4.4 %). Results demonstrated that all these pectic polysaccharides significantly mitigated dextran sulfate sodium-induced UC through upregulating tight junction proteins, suppressing intestinal pro-inflammatory cytokines, promoting short-chain fatty acid production, and resorting gut microbiota homeostasis. Notably, low-DE QMP-LDE demonstrated superior efficacy to high-DE QMP-HDE in restoring intestinal barrier integrity (enhanced upregulation of tight junction proteins and reduced serum lipopolysaccharide) and in elevating anti-inflammatory cytokine interleukin-10. Although all pectic polysaccharides suppressed pathogenic genera ( Escherichia-Shigella, Klebsiella, and Helicobacter ), they differentially modulated beneficial bacteria ( Lactobacillus and Muribaculaceae). QMP-HDE selectively enhanced Lactobacillus abundance, whereas QMP-LDE specifically enriched Muribaculaceae. These differential effects among QMP-HDE and QMP-LDE are closely linked to their DE levels. Collectively, our findings shed light on the structure-anti-UC activity relationship of QMP pectic polysaccharides, supporting their potential application as functional foods to prevent intestinal inflammatory disorders.
研究领域:
食品科学与技术
·
营养生物化学
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免疫学
·
胃肠病学
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微生物组研究
·
多糖化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）