SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
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IF 4.6
Yuxin Tian,Yuwei Du,Zhang Chen,Li Li,Ruhong Yan,Xiaoqiang Li,Juan Yue
FRET, known as Förster Resonance Energy Transfer, serves as a versatile technique enabling precise quantitative assessment of protein concentrations with minimal disruption to biological processes. In this study, we have developed two types of fluorescent carbon quantum dots, B-CDs and G-CDs, serving as donor and acceptor probes, respectively, for FRET-based procalcitonin (PCT) detection. The B-CDs exhibit strong blue fluorescence (excitation/emission peaks at 360/442 nm) as donors, while the G-CDs display faint green fluorescence (excitation/emission peaks at 449/520 nm) as acceptors. These CDs are conjugated with PCT antibodies to form immune probes. Upon antigen binding, the antibody quickly binds and reacts with the antigen. Concurrently, the fluorescence energy from the donor is transferred to the acceptor, leading to a decrease in the blue fluorescence intensity emitted by B-CDs. The ratio of the fluorescence intensity between the green probe (G-CDs-Ab2) and the blue probe (B-CDs-Ab1) exhibited a positive correlation with the concentration of PCT antigen in the low concentration range of 0.5 to 5 ng/mL. Consistent results were observed in both phosphate-buffered saline (PBS) and diluted human serum. In the concentration range of 0.5–5 ng/mL in PBS buffer, the linear equation is expressed as F A /F D = −0.0045 × c [PCT] ^2 + 0.0021 × c [PCT] + 0.3232, with a limit of detection (LOD) of 0.1 ng/mL based on 3σ K −1 . For concentrations spanning from 5-80 ng/mL, the equation is F A /F D = 0.0021 × c [PCT] + 0.4212. These findings suggest that carbon dot-labeled immune probes could offer a novel approach for sensitive detection of disease biomarkers.
研究领域:
生物化学
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医学检验
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纳米技术
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DOI
PubMed
高影响因子
Advanced Science
·
2023
·Vol. 10
·期 15
·2207437
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IF 15.1
Jianying Jing,Kun Liu,Junfeng Jiang,Tianhua Xu,Lu Xiao,Xiaohan Zhan,Tiegen Liu
The detection of trace biomarkers is an important supplementary approach for early screening and diagnoses of tumors. An optical fiber near-field enhanced plasmonic resonance immunoprobe is developed for the detection of the hepatocellular carcinoma biomarker, i.e., the alpha-fetoprotein. Generic principles based on dispersion models and finite element analysis (FEA) models are developed to realize the optimized configuration of spectral characteristics of the immunoprobe. Dispersion models provide theoretical guidance for the design of the multilayer sensing structure from the perspective of the ray optics theory. FEA models provide theoretical guidance for the selection of coating materials from the perspective of the self-defined dielectric constant ratio, i.e., the ratio of the real part to the imaginary part. The optimized configuration of the antibody coupling further improves the biosensing performance of the immunoprobe. The limit of detection (LOD) can reach down to 0.01 ng mL −1 , which is one order of magnitude lower than those relevant reported works. Such a low LOD can more effectively avoid the accuracy degradation of detection results due to measurement errors. Human serum samples have also been detected, with the good precision achieved. This work shows promising prospects in applications of label-free, low-cost, rapid, and convenient early screening of tumors.
研究领域:
生物医学工程
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光学
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癌症诊断
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DOI
PubMed
高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2014
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IF 2.829
Yajing Lei,Xiaoyi Li,Muhammad Sajid Hamid Akash,Lifang Zhou,Xiaojing Tang,Weixing Shi,Zhiming Liu,Shuqing Chen
Salbutamol (SAL) is known as quick-acting β-2 receptor agonist and its use in humans for pulmonary diseases and/or in animal feed is limited because of associated potential hazardous effects on health. Several techniques are available for the detection of SAL, but are expensive and time consuming. Here for the first time, a novel pre-assembled DNA immunoprobe-based immuno-PCR assay was developed to investigate the levels of SAL in human urine samples and compared the proposed immuno-PCR assay for the detection of SAL with that of direct competitive ELISA. Under the optimized conditions, the assay showed a linear range over seven orders of magnitude, whereas the limit of detection of SAL in buffer was found to be 21 fg/mL. Current immuno-PCR assay exhibited a 300-fold better detection limit for SAL as compared to one achieved with direct competitive ELISA using the same antibody. In conclusion, it has been found that the developed method is highly sensitive and simple method that can significantly enhance the detection sensitivity for small molecules. Moreover it can be modified and used for detecting other small molecules and/or chemicals that exist in the environment and are responsible for the environmental pollution.
研究领域:
分析化学
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分子生物学
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环境科学
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DOI
PubMed
高影响因子
Journal of Materials Chemistry C
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2019
·Vol. 8
·期 6
·2142-2154
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IF 6.641
Zhiyuan Yang,Hongmei Liu,Yiran Tian,Ying Chen,Zhiqiang Niu,Canliang Zhou,Fuyan Wang,Chenjie Gu,Shiwei Tang,Tao Jiang,Jun Zhou
In the field of surface-enhanced Raman scattering (SERS)-based immunoassays utilizing a sandwich structure, one of the main barriers to overcome is the development of an immunoprobe and substrate with ultrahigh sensitivity and relatively good reproducibility. In this work, a novel gold (Au)-coated “stellate” mesoporous SiO2 (SMSiO2) hybrid structure was proposed and applied as an immunoprobe. It was noticed that the SMSiO2 matrix with an appropriate coating amount of Au nanoparticles (NPs) demonstrated a much higher SERS intensity than solid SiO2 (SSiO2)@Au NPs with an enhancement ratio of 2.6. Electromagnetic “hot spots” existed around the sharp edges and ultrasmall nanogaps of the multibranched SiO2 matrix were verified to be the main reason responsible for the conspicuous enhancement given by the simulation results. Meanwhile, a hydrophilic–hydrophobic SERS-active immunosubstrate was prepared by linking antibodies onto the hydrophilic Ag-deposited sandpaper assembled with hydrophobic-treated filter paper. Such a novel substrate maintained excellent SERS homogeneity with quite a low relative standard deviation (RSD) of 12.85% arising from the elimination of the coffee-ring effect. The constructed sandwich immunoassay structure using the developed immunoprobe and the substrate was successfully employed in a specific assay for the ferritin (FER) antigen and exhibited an extensive dynamic linear range from 1 × 10−5 to 3 × 10−13 g mL−1 with an ultralow limit of detection (LOD) of 3.16 × 10−14 g mL−1. The simplicity and versatility of this strategy demonstrated its superior sensitivity for tumor markers and it can be expected to have potential practical application in the clinical diagnoses of cancer.
研究领域:
生物医学工程
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分析化学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
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IF 5.667
Weixiang Li,Di Shu,Hongliang Han,Zhanfang Ma
Nanogel as a multifunctional carrier was used to fabricate immunoprobe. The obtained immunoprobe was applied in a competitive amperometric immunosensor and exhibited an excellent analytical performance for the detection of tumor marker. The nanogel was easily fabricated by irradiation of the mixture of glucose, sodium citrate and sodium alginate by microwave, which exhibits excellent adsorbent ability for multivalence metal ions. The immunoprobe was constructed by functionalizing such uniform nanogels with multivalence metal ions (Cu 2+ as model) as redox species and gold nanoparticles for immobilizing antibody. The signal of Cu 2+ from the nanogel can be directly measured without acid dissolution and pre-concentration, which substantially simplified the detection steps. Besides, the response current can be amplified by glucose because Cu 2+ is an electro-catalyst for the oxidation of glucose. Polyaniline hydrogel with a three dimensional network structure and π–π conjugated structure was used to fabricate immunosensing substrate, which extremely improved the specific surface area and conductivity of the modified electrode. Under optimal conditions, the current changes of square wave voltammetry were used to detect neuron specific enolase (as a model analyte) with a broad linear ranging from 0.01 to 1000 ng mL −1 and a ultralow detection limit of 4.6 pg mL -1 (S/N = 3). This work provides a new strategy for designing multifunctional immunoprobes.
研究领域:
生物医学工程
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分析化学
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肿瘤学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2021
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IF 7.46
Ziluo Su,Hongmei Liu,Ying Chen,Chenjie Gu,Guodong Wei,Tao Jiang
Aiming to augment the reliability and efficiency of surface enhanced Raman scattering (SERS)-active immunoprobe, sophisticated strategies that are able to avoid the dissociation of Raman molecules and noble metal nanoparticles (NPs) in practice have drawn a considerable attention. This study confirmed that Ag@polystyrene-block-poly(acrylic acid)@Ag core-shell nanostructures possessed superior stability compared with bare Ag NPs in phosphate buffer saline solution and human serum. Combined with a unique paper-based immunosubstrate with a satisfying enhancement factor of 9.71 × 10 5 , a robust sandwich immunostructure was constructed and conducted to monitor carbohydrate antigen 19-9 (CA19-9), presenting a low limit of detection of about 10 −4 U/mL. With these exceptional features, such a proposed protocol was finally utilized to detect CA19-9 in several clinical human sera, which shown much higher sensitivity than the traditional chemiluminescence analysis.
研究领域:
分析化学
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生物医学工程
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诊断学
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DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
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2023
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IF 12.6
Xi Mei,Gan Zhang,Tingting Liu,Hong Hu,Xiaocui Lai,Wenyao Chen,Yumeng Wang,Juan Peng,Weihua Lai
Immunochromatographic assay (ICA) has attracted widespread attention owing to its advantages of economy, simplicity, and rapidity. However, the synthesis of immunoprobes is still limited by complicated design ideas and multistep operations from preparing nanoparticles to conjugating monoclonal antibodies (mAb) onto nanoparticles. Inspired by the biomineralization of zeolitic imidazolate framework-8 (ZIF-8), we proposed a strategy for the rapid synthesis of an integrated immunoprobe (ZIF-8@QDs-mAb), achieving a one-step integration with strong fluorescent signal output capability and specific recognition ability. In addition, different fluorescent colors of ZIF-8@QDs-mAb were generated by doping red and green quantum dots (QDs) in various ratios. With a smart detection platform, the developed ZIF-8@QDs-mAb-based multiplex ICA (ZIF-8@QDs-mAb-mICA) achieved the on-site quantitative detection of enrofloxacin , sulfamethazine , and kanamycin in milk within 15 min, with the limit of detection (LOD) of 0.052, 0.186 and 0.216 ng mL −1 , which were 5.69, 2.20 and 4.40 times higher than that of gold nanoparticles-based mICA, respectively. The quantitative detection of alpha-fetoprotein and human chorionic gonadotropin was also achieved with LOD of 0.516 ng mL −1 and 0.225 mIU mL −1 , respectively, which verified the universality of the strategy. This work provides a novel idea for the design of an efficient integrated immunoprobe and has broad application prospects in ICA.
研究领域:
分析化学
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纳米技术
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生物医学工程
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食品安全
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产品关联
DOI
PubMed
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2017
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IF 2.536
Qiaoqiao Shi,Jie Huang,Yaning Sun,Mengqi Yin,Mei Hu,Xiaofei Hu,Zhijun Zhang,Gaiping Zhang
An ultrasensitive method for the detection of antibiotics in milk is developed based on inexpensive, simple, rapid and portable lateral flow immunoassay (LFI) strip, in combination with high sensitivity surface-enhanced Raman spectroscopy (SERS). In our strategy, an immunoprobe was prepared from colloidal gold (AuNPs) conjugated with both a monoclonal antibody against neomycin (NEO-mAb) and a Raman probe molecule 4-aminothiophenol (PATP). The competitive interaction with immunoprobe between free NEO and the coated antigen (NEO-OVA) resulted in the change of the amount of the immobilized immunoprobe on the paper substrate. The LFI procedure was completed within 15 min. The Raman intensity of PATP on the test line of the LFI strip was measured for the quantitative determination of NEO. The IC 50 and the limit of detection (LOD) of this assay are 0.04 ng/mL and 0.216 pg/mL of NEO, respectively. There is no cross-reactivity (CR) of the assay with other compounds, showing high specificity of the assay. The recoveries for milk samples with added NEO are in the range of 89.7%–105.6% with the relative standard deviations (RSD) of 2.4%–5.3% ( n = 3). The result reveals that this method possesses high specificity, sensitivity, reproducibility and stability, and can be used to detect a variety of antibiotic residues in milk samples.
研究领域:
分析化学
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食品安全
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生物技术
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DOI
PubMed
高影响因子
Scientific Reports
·
2016
·Vol. 6
·期 1
·1-6
·
IF 5.228
Tang Zhongxue,Ma Zhanfang
In this work, we presented a ratiometric electrochemical immunosensor based on redox substrate and immunoprobe. Carboxymethyl cellulose-Au-Pb 2+ (CMC-Au-Pb 2+ ) and carbon-Au-Cu 2+ (C-Au-Cu 2+ ) nanocomposites were firstly synthesized and implemented as redox substrate and immunoprobe with strong current signals at −0.45 V and 0.15 V, respectively. Human immunoglobulin G (IgG) was used as a model analyte to examine the analytical performance of the proposed method. The current signals of CMC-Au-Pb 2+ (I substrate ) and C-Au-Cu 2+ (I probe ) were monitored. The effect of redox substrate and immunoprobe behaved as a better linear relationship between I probe /I substrate and Lg C IgG (ng mL −1 ). By measuring the signal ratio I probe /I substrate , the sandwich immunosensor for IgG exhibited a wide linear range from 1 fg mL −1 to 100 ng mL −1 , which was two orders of magnitude higher than other previous works. The limit of detection reached 0.26 fg mL −1 . Furthermore, for human serum samples, the results from this method were consistent with those of the enzyme linked immunosorbent assay (ELISA), demonstrating that the proposed immunoassay was of great potential in clinical diagnosis.
研究领域:
分析化学
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生物传感器
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电化学
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免疫学
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产品关联
DOI
PubMed
高影响因子
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