MICROCHIMICA ACTA
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2025
·Vol. 193
·期 1
·28
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IF 5.3
Li Yixian,Yang Xiaohui,Cheng Shanshan,Mu Rongrong,Li Huiqiang,Yu Yang
Autoantibodies play a critical role in predicting diseases and monitoring therapeutic outcomes. Limitations of current autoantibody detection techniques include prolonged detection times and operational complexity. This study aims to develop a bidirectional microfluidic platform for the detection of autoantibodies. A “double inlet” and “waste outlet” structure was developed on the bidirectional lateral-flow microfluidic chip (BiLFMC). Using anti-citrullinated peptide antibodies (ACPA) as a model, this study validated the feasibility and performance advantages of the BiLFMC in the diagnosis of autoimmune disease. The BiLFMC was optimized by selecting the optimal distances between the sample inlet and waste outlet, as well as the ideal diameter for the waste outlet. The most suitable absorbent material was chosen for the waste absorbent pad. The detection and anti-interference performance of the unidirectional mode, unidirectional sequential mode, and bidirectional mode were compared, demonstrating that the bidirectional microfluidic chip significantly enhanced anti-interference capabilities. For ACPA detection, a serum sample required only a 1:100 dilution and 10 μL volume, with results available in 8 min. The coefficient of variation (CV) for repeatability ranged from 7.04% to 13.55%, while the CV for intermediate precision ranged from 11.06% to 14.49%. The limit of quantitation was 3.951 RU/mL, and the upper limit of the linear range was 500 RU/mL. When compared to ELISA results, the consistency coefficient was 0.8302. Furthermore, the BiLFMC was able to accurately distinguish samples exceeding the ELISA upper limit (> 196 RU/mL). A bidirectional microfluidic chip has been developed, incorporating indirect detection methods to enhance the anti-interference capability of the microfluidic platform. Graphical abstract
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高影响因子
Frontiers in Microbiology
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2021
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IF 5.64
Wang Wenbin,Liu Jianxin,Guo Shanshan,Liu Lei,Yuan Qianyun,Guo Lei,Pan Saikun
The discovery of outer membrane proteins (OMPs) with desirable specificity and surface availability is a fundamental challenge to develop accurate immunodiagnostic assay and multivalent vaccine of pathogenic Vibrio species in food and aquaculture. Herein 101 OMPs were systemically screened from 4,831 non-redundant proteins of Vibrio parahaemolyticus by bioinformatical predication of signaling peptides, transmembrane (TM) α-helix, and subcellular location. The sequence homology analysis with 32 species of Vibrio spp. and all the non-Vibrio strains revealed that 15 OMPs were conserved in at least 23 Vibrio species, including BamA (VP2310), GspD (VP0133), Tolc (VP0425), OmpK (VP2362), OmpW (VPA0096), LptD (VP0339), Pal (VP1061), flagellar L-ring protein (VP0782), flagellar protein MotY (VP2111), hypothetical protein (VP1713), fimbrial assembly protein (VP2746), VacJ lipoprotein (VP2214), agglutination protein (VP1634), and lipoprotein (VP1267), Chitobiase (VP0755); high adhesion probability of flgH, LptD, OmpK, and OmpW indicated they were potential multivalent Vibrio vaccine candidates. V. parahaemolyticus OMPs were found to share high homology with at least one or two Vibrio species, 19 OMPs including OmpA like protein (VPA073), CsuD (VPA1504), and MtrC (VP1220) were found relatively specific to V. parahaemolyticus. The surface proteomic study by enzymatical shaving the cells showed the capsular polysaccharides most likely limited the protease action, while the glycosidases improved the availability of OMPs to trypsin. The OmpA (VPA1186, VPA0248, VP0764), Omp (VPA0166), OmpU (VP2467), BamA (VP2310), TolC (VP0425), GspD (VP0133), OmpK (VP2362), lpp (VPA1469), Pal (VP1061), agglutination protein (VP1634), and putative iron (III) compound receptor (VPA1435) have better availability on the cell surface.
研究领域:
微生物学
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分子生物学
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免疫学
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生物信息学
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食品科学
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水产养殖
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PubMed
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2025
·
IF 11.3
Wanchao Zuo,Zhipeng Ding,Haodong Zhao,Xiangming Meng,Jiaren Song,Qing Yang,Siqi Zeng,Tianye Fang,Jianjun Dai,Feng Xue,Yanmin Ju
Early detection of pathogenic bacterial infections is crucial for mitigating risks, yet conventional assays face limited adoption due to their dependence on laborious protocols, specialized instruments/personnel, and slow turnaround times. While colorimetric lateral flow immunoassays (CLFIA) offer an efficient and convenient alternative, their sensitivity remains insufficient for detecting early-stage bacterial contamination primarily constrained by inadequate colorimetric signal intensity. Here, we integrate defect-rich intermetallic Pt 3 Sn nanocrystals ( D -Pt 3 Sn NCs) as colorimetric signal amplifiers to develop an enzyme-accelerated signal enhancement (EASE)-based CLFIA for the detection of early-stage bacterial contamination. D -Pt 3 Sn NCs exhibit enhanced peroxidase-like activity than Pt 3 Sn NCs without surface depressions, and superior tolerance and stability under extreme environments relative to natural horseradish peroxidase. Upon integration with the EASE-based CLFIA platform, D -Pt 3 Sn NCs enable sensitive immunochromatographic analysis of Escherichia coli O157:H7 ( E. coli O157:H7) with a visual detection sensitivity of 10 CFU/mL. Besides, proof-of-concept assays for detecting E. coli O157:H7 in cabbage and beef samples validate the anti-matrix interference capability and practicality of this platform. Overall, defect-rich intermetallic nanocrystals hold promise as versatile catalytic colorimetric signal amplifiers, enabling highly-sensitive immunochromatographic detection of trace-level analytes in complex matrices.
研究领域:
纳米技术
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生物传感器
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分析化学
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食品安全
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免疫诊断
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PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
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2025
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IF 3.8
Li Cuili,Xiang Maojie,Liu Jie,Zhang Shuting,Li Haojie,Fu Guanglei
Pressure-based detection has drawn increasing interest for developing immunoassays but is still challenged in point-of-care testing (POCT). In this work, a pressure-based immunoassay “lollipop” was developed using syringe-autoinjected visual distance readout. The “lollipop” consisted of a disposable syringe “stick” as the immuno-recognition host and a chip “head” as the signal readout element. Using urinary microalbuminuria (mALB) as a model target, we constructed the immunoassay system on the interior cylinder surface of the syringe. In the presence of H 2 O 2 , the peroxidase-mimicking activity of platinum nanoparticles (Pt NPs) immuno-captured in the cylinder drove the automatic injection of dye liquids from the syringe into a spiral capillary catheter in the chip. The concentration of mALB in the “lollipop stick” was thereby visually displayed as the syringe-autoinjected distance signals in the “lollipop head”. The assay exhibited a limit of detection (LOD) of 5 ng/mL for mALB quantification and linear response in a range from 0.006 μg/mL to 6 μg/mL with reliable accuracy in clinical urine sample analysis. Taking advantage of the versatility of syringes, the immunoassay “lollipop” works without the aid of any laboratory liquid handlers (e.g., pipettes), specific detectors (e.g., manometers) and complex chip operations (e.g., reagent loading), showing great promise in POCT.
研究领域:
生物分析化学
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即时检测
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免疫诊断
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纳米技术
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微流控技术
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DOI
PubMed
高影响因子
TALANTA
·
2016
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IF 4.035
Kun Xiao,Kan Wang,Weijian Qin,Yafei Hou,Wenting Lu,Hao Xu,Yan Wo,Daxiang Cui
Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity , high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378 ng/mL and 0.0426 ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis.
研究领域:
肿瘤学
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免疫诊断
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纳米生物技术
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即时检测
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临床化学
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DOI
高影响因子
危险品化学品经营许可证（带存储）