IEEE SENSORS JOURNAL
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2024
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IF 4.3
Tengfei Yang,Pengxiang Chang,Ailing Zhang,Fei Liu,Honggang Pan,Junfeng Wang,Guangxiao Cao,Mingxuan Zheng,Zhao Zhang
A PDA/AuNPs/PDANPs sandwich structure modified optical fiber surface plasmon resonance (SPR) biosensor for specific detecting of rabbit IgG were proposed in this paper. On the proposed sensor, polydopamine (PDA), gold nanoparticles (AuNPs) and polydopamine nanoparticles (PDANPs) were sequentially deposited to form a multilayer hybrid structure. The refractive index (RI) sensitivity of the sensor has been significantly enhanced through the strong coupling effects of SPR and localized surface plasmon resonance. The sensor achieves an RI sensitivity of3009.58 nm/RIU, representing a 3-fold increase compared to the original SPR sensor. In addition, due to the high surface activity and large specific surface area of PDANPs, the biosensitivity of the sensor has been enhanced. The sensor demonstrated specific detection of Rabbit IgG with a biosensitivity of 0.9157 nm/(μg/mL) and limit of detection (LOD) of 22 ng/mL. These findings indicate that the prepared sensor can specifically detect Rabbit IgG with high precision and a low LOD. Therefore, this PDA/AuNPs/PDANPs sandwich structure modified biosensor has great potential in clinical detection and other bio-detection fields.
研究领域:
生物传感
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纳米技术
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医学检测
✓ 已验证
产品关联
DOI
高影响因子
Biosensors-Basel
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2024
·Vol. 14
·期 7
·322
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IF 4.9
Feng Wang,Lukai Ma,Qin Wang,Bruce D. Hammock,Gengsheng Xiao,Ruijing Liu
Patulin, an emerging mycotoxin with high toxicity, poses great risks to public health. Considering the poor antibody production in patulin immunization, this study focuses on the four-dimensional data-independent acquisition (4D-DIA) quantitative proteomics to reveal the immune response of patulin in rabbits. The rabbit immunization was performed with the complete developed antigens of patulin, followed by the identification of the immune serum. A total of 554 differential proteins, including 292 up-regulated proteins and 262 down-regulated proteins, were screened; the differential proteins were annotated; and functional enrichment analysis was performed. The differential proteins were associated with the pathways of metabolism, gene information processing, environmental information processing, cellular processes, and organismal systems. The functional enrichment analysis indicated that the immunization procedures mostly resulted in the regulation of biochemical metabolic and signal transduction pathways, including the biosynthesis of amino acid (glycine, serine, and threonine), ascorbate, and aldarate metabolism; fatty acid degradation; and antigen processing and presentation. The 14 key proteins with high connectivity included G1U9T1, B6V9S9, G1SCN8, G1TMS5, G1U9U0, A0A0G2JH20, G1SR03, A0A5F9DAT4, G1SSA2, G1SZ14, G1T670, P30947, P29694, and A0A5F9C804, which were obtained by the analysis of protein–protein interaction networks. This study could provide potential directions for protein interaction and antibody production for food hazards in animal immunization.
研究领域:
毒理学
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免疫学
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蛋白质组学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）