CHINESE JOURNAL OF POLYMER SCIENCE
·
2022
·Vol. 41
·期 1
·40-50
·
IF 3.815
Wu Xin-Yu,Yang Jun,Wu Fang-Hui,Cao Wang-Bei,Zhou Tong,Wang Zhao-Yi,Tu Chen-Xi,Gou Zhong-Ru,Zhang Lei,Gao Chang-You
The dense networks of conventional hydrogel hinder the efficient cell penetration and thus tissue regeneration. In this study, a macroporous cryogel with excellent mechanical properties was designed and fabricated through cryogelation and crosslinking of methacrylated gelatin (Gel-MA) and gelatin grafted with 2-ureido-4[1 H ]-6-methyl-pyrimidinone (Gel-UPY). The successful modification of the two functional units on the side chain of gelatin was confirmed by 1 H-NMR and infrared spectroscopy. The macroporous structure of cryogel was verified by scanning electron microscopy and confocal microscopy. The compressive modulus of cryogel (110 kPa) was 2 magnitudes higher than that of the conventional hydrogel of the same chemical compositions (8 kPa). Both the hydrogel and cryogel exhibited excellent stress-recovery ability. The cryogel could well maintain the viability of chondrocytes and facilitated their migration into the scaffold interior. After being implanted into osteochondral defects in rabbits for 12 w, the Gel-MA/Gel-UPY cryogel better facilitated the regeneration of upper cartilage and subchondral bone compared to the conventional hydrogel.
研究领域:
生物材料学
·
再生医学
·
骨科研究
✓ 已验证
产品关联
DOI
高影响因子
Heliyon
·
2024
·
IF 3.4
Yingjie Wu,Mingxiang Fan,Shixiang Tan,Qiucheng Guo,Hegui Xu
Objective To examine and talk about the mechanism of the Huoxue Jiegu compound capsule's effects on osteoblasts and the PI3K/Akt/mTOR signal pathway in rabbits suffering from tibial fractures. Method In vitro, CCK8 was used to assess the survival rates. Alizarinred staining was used to evaluate mineralized nodules. ALP staining was used to observe the osteoblasts. qRT-PCR was used to determine the mRNA expression of the bone formation-related factors BMP-2, bFGF, and TGF-β. In vivo, three groups of nine male rabbits each were randomly assigned to three groups: the Model group, the Huoxue Jiegu compound capsule group (HXJGC group), and the inhibitor group (HXJGC+3-MA), four weeks following the intervention. HE staining was employed to examine the rabbits' bone histology. immunohistochemistry was employed to examine the relative expression of the proteins VEGF and LC3-II. Western Blot was utilized to examine the relative expression of the proteins Beclin-1, LC3-II/Ⅰ, p62, p-PI3K, p-AKT, and p-mTOR. Results Compared to the control group, the medium- and high-dose groups exhibited considerably higher survival rates ( P < 0.05), as well as enhanced cell proliferation and differentiation ( P < 0.05) and more pronounced mineralized nodules. ( P < 0.05), but the low-dose groups showed no appreciable variation. In the low, medium, and high-dose groups, there was a substantial reduction in the expression of bFGF mRNA, whereas the levels of BMP-2 and TGF-β mRNA were considerably higher than in the control group ( P < 0.05). In vivo, after four weeks of treatment, the model control group and inhibito group had a large amount of fibrous hyperplasia accompanied by bleeding and a small amount of inflammatory cell infiltration. But in the HXJGC group, new cartilage appeared, and the surface of the cartilage was smooth and flat. Beclin-1 and LC3-II/I expression in the HXJGC+3 MA group was significantly lower than in the HXJGC and Model groups ( P < 0.05). The HXJGC group showed lower p62 expression than the HXJGC+3 MA and model groups ( P < 0.05). The HXJGC group exhibited significantly reduced levels of p-PI3K, p-AKT, and p-mTOR expression in comparison to HXJGC+3 MA groups ( P < 0.05). Conclusion Rabbits with tibial fractures can be treated with HXJGC, which can control the expression of the PI3K/Akt/mTOR signal pathway. It can promote the differentiation and maturation of osteoblasts at the fracture end of rabbits, accelerate the recovery of fractures, and achieve the purpose of treating the disease.
研究领域:
医学
·
生物学
✓ 已验证
DOI
PubMed
高影响因子
TOXICOLOGY AND APPLIED PHARMACOLOGY
·
2017
·
IF 3.791
Gang-Feng Ge,Wei-Wen Shi,Chen-Huan Yu,Xiao-Yin Jin,Huan-Huan Zhang,Wen-You Zhang,Lu-Chen Wang,Bing Yu
Chemotherapy is one of the major strategies for cancer treatment. Several antineoplastic drugs including vinorelbine (VRB) are commonly intravenously infused and liable to cause serious phlebitis. The therapeutic drugs for preventing this complication are limited. In this study, the mechanism of baicalein (BCN) was investigated on VRB-induced phlebitis in vivo and vascular endothelial cell injury in vitro . Treatment with BCN obviously attenuated vascular endothelial cell loss, edema, inflammatory cell infiltration and blood clots, and reduced the serum levels of TNF-α, IL-1β, IL-6 and ICAM-1 in the rabbit model of phlebitis induced by intravenous injection of VRB compared with vehicle. Further tests in vitro demonstrated that BCN lessened VRB-induced endothelial cell apoptosis, decreased intracellular ROS levels, suppressed phosphorylation of p38 and eventually inhibited activation of NF-κB signaling pathway. And these effects could be reversed by p38 agonist P79350. These results suggested that BCN exerted the protective effects against VRB-induced endothelial disruption in the rabbit model of phlebitis via inhibition of intracellular ROS generation and inactivation of p38/NF-κB pathway, leading to the decreased production of pro-inflammatory cytokines. Thus, BCN could be used as a potential agent for the treatment of phlebitis.
研究领域:
肿瘤学
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药理学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Chemistry
·
2024
·
IF 3.8
Sun Jingxuan,He Lina,An Qi,Ye Xu,Ma Jinjie,Yan Jing,Xie Xiaoqi,Sun Xiangyu,Niu Yumei,Cao Wenxin
Introduction: Facial nerve injury significantly impacts both the physical and psychological] wellbeing of patients. Despite advancements, there are still limitations associated with autografts transplantation. Consequently, there is an urgent need for effective artificial grafts to address these limitations and repair injuries. Recent years have witnessed the recognition of the beneficial effects of chitosan (CS) and graphene in the realm of nerve repair. Dental pulp stem cells (DPSCs) hold great promise due to their high proliferative and multi-directional differentiation capabilities.Methods: In this study, Graphene/CS (G/CST) composite tubes were synthesized and their physical, chemical and biological properties were evaluated, then DPSCs were employed as seed cells and G/CST as a scaffold to investigate their combined effect on promoting facial nerve injury repair.Results and Disscussion: The experimental results indicate that G/CST possesses favorable physical and chemical properties, along with good cyto-compatibility. making it suitable for repairing facial nerve transection injuries. Furthermore, the synergistic application of G/CST and DPSCs significantly enhanced the repair process for a 10 mm facial nerve defect in rabbits, highlighting the efficacy of graphene as a reinforcement material and DPSCs as a functional material in facial nerve injury repair. This approach offers an effective treatment strategy and introduces a novel concept for clinically managing facial nerve injuries.
研究领域:
生物医学工程
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组织工程
·
干细胞研究
·
神经科学
✓ 已验证
DOI
PubMed
高影响因子
American Journal of Translational Research
·
2021
·
IF 4.06
Lexiang Li,Yi Chen,Qiwei Fu,Haishan Wu,Yiqin Zhou,Jiahua Shao,Jun Wu,Yaguang Han,Qirong Qian
Background: Tissue engineering is widely applied to treat osteochondral damage in osteoarthritis (OA). However, the superposition of seed cells, material scaffolds, inducing factors, and microenvironmental factors limit their practical application. We intended to develop a novel tissue engineering method for improving the repairment of osteochondral damage and to discuss its effect on repairing osteochondral defects. Methods: The combined decellularization methods of physics, chemistry and enzymes were used to decellularize rabbit rib cartilage and articular cartilage, and rabbit decellularizated osteochondral composite scaffolds were prepared. The structure and organization of the scaffolds were analyzed. We extracted and identified infrapatellar fat pad stem cells (IPFP-SCs) from healthy rabbits and OA rabbit, which were different in viability, migration, osteogenic and chondrogenic differentiation. Finally, a variety of decellularizated bone cartilage composite scaffolds were loaded with rabbit IPFP-SC for in vitro and in vivo studies. Results: The decellularization effect was strong, and the organic ingredients were lost. The layered scaffold showed lower density, greater porosity, larger pore size and water absorption than the whole scaffold, but the mechanical properties of the two scaffolds were low. IPFP-SCs were successfully extracted, and the migration and cartilage ability of IPFP-SCs in OA group were weak. The decellularized scaffold showed a high biocompatibility. The structure and composition of osteochondral promoted osteogenic differentiation and chondrogenic differentiation of IPFP-SCs. Moreover, the decellularized extracellular matrix loaded with IPFP-SC had the strongest repairing effect. Conclusion: The decellularized extracellular matrix loaded with IPFP-SC showed a better repair effect on rabbit osteochondral defects.
研究领域:
组织工程
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骨科
·
干细胞研究
·
再生医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Qiangjun Kang,Guohui Liu,Changan Liu,Weina Ren,Yuqing Wang
Bone defects are common consequences of bone trauma. The application of biomaterials as tissue scaffolds combined with oxygenating agents, as bone regeneration promoters, is an interesting strategy to improve bone repair. The present study aimed to investigate the potential of a temperature-sensitive hydrogel containing 1-bromoheptafluorooctane in repairing rabbit bone defects and its possible mechanism of action. Bone defects were surgically created in the femoral condylar of New Zealand White rabbits. The animals were divided into three groups: A) treated with the temperature-sensitive hydrogel containing 1-bromoheptafluorooctane with oxygen release; B) treated with the temperature-sensitive hydrogel containing 1-bromoheptafluorooctane without oxygen release; and C) received no treatments. At four and 12 weeks post-operation, the specimens from all groups were collected. The new bone formation was studied by morphological observation, X-ray, Micro-CT, Masson’s trichrome, and CD31 immunohistochemical staining. At 12 weeks post-operation, the bone defects were completely healed in groups A and B, while a major bone defect was still present in group C. Micro-CT showed that TMD, BVF, Tb.Th and Tb.N in group A were significantly higher compared to the other groups at each time point post-operation. The SMI value of group A showed a significant difference from the other groups at 12 weeks post-operation. The number of micro-vessels in group A was significantly higher compared to the other groups at four and 12 weeks post-operation. The oxygenating temperature-sensitive hydrogel containing 1-bromoheptafluorooctane could effectively promote new bone formation while increasing tissue vascularization, highlighting a good clinical application prospect.
研究领域:
生物医学工程
·
组织工程
·
骨科
·
生物材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION
·
2023
·
IF 2.7
Gongyan Liu,Haitao Sun,Ce Liu,Liya Bai,Liping Yang,Wenxue Jiang,Shuxia Gao
This study was conducted to investigate the effects of different dietary fibre sources on growth performance, gastrointestinal tract development, caecal fermentation and bacterial composition in the caecal contents of rabbits. A total of 120 35-day-old weaned Minxinan black rabbits were divided into three groups and fed a diet composed of peanut straw powder (Group A), alfalfa powder (Group B) and soybean straw powder (Group C) as the main fibre source. The final body weight and average daily gain in Group B were higher than those in Group C, and the average daily feed intake and feed conversion ratio in Group A were lower than those in Group C ( p < 0.05). The relative weights of the stomach, small intestine and caecum of rabbits in Group C were higher than those in Groups of B and A, and the relative weights of the caecal contents in Group C were lower than those in Groups A or B ( p < 0.05). The pH value and propionic acid, butyric acid and valeric acid concentrations in the caecum of Group C were lower than those in the caecum of Groups A or B, and the concentration of acetic acid in the caecum was lower ( p < 0.05). The dominant microbes in the caecal contents of Minxinan black rabbits were Firmicutes, Bacteroidetes and Proteobacteria at the phylum level, and the number of species, Chao1 index and ACE index measured was different between the B–C and A–C groups ( p < 0.05). Different dietary fibre sources could affect the growth performance, gastrointestinal tract development and intestinal microbiota of rabbits, and the nutritional value of alfalfa powder was better than that of peanut straw and soybean straw.
研究领域:
动物营养
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微生物学
·
胃肠生理学
✓ 已验证
DOI
PubMed
高影响因子
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
·
2025
·
IF 6.1
Xuejun Yuan,Wenjie Chen,Rui Li,Huijun Guo,Chunyang Wang
Deoxynivalenol (DON) is one of the most prevalent mycotoxins and can induce weight loss and vomiting in animals. This study aimed to elucidate the molecular mechanisms underlying DON-induced anorexia in rabbits through the microbial-gut-brain axis. A total of forty rabbits were randomly divided into a DON group and a control group, receiving 1.5 mg/kg.BW of DON and an equivalent volume of saline daily via intragastric administration, respectively. The pre-treatment period lasted for 7 days, followed by a formal experimental period of 24 days. The findings revealed that DON exposure significantly reduced daily food intake and weight gain, leading to intestinal apoptosis and barrier injuries. 16S rRNA sequencing data indicated that DON exposure decreased the diversity and richness of cecal microflora, suppressed probiotic populations, and disrupted host lipid metabolism. RNA-Seq and iTRAQ data were used to cross-analyze the shared differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) in the hypothalamus and jejunum, along with their shared enrichment pathways, indicating that TNF-α, NF-κB, and NPY may play pivotal roles in DON-induced anorexia. qRT-PCR and Western blotting results confirmed significant increases in mRNA and protein expression levels of TNF-α, NF-κB and 5-HT2A in both the hypothalamus and jejunum. In conclusion, DON ingestion in rabbits can disrupt the intestinal barrier, up-regulate the expression of IFN-γ, TNF-α, NF-κB, and 5-HT2A in the intestine, and trigger an immuno-inflammatory response in the hypothalamus via the microbial-gut-brain axis, ultimately resulting in appetite suppression and weight loss.
研究领域:
毒理学
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分子生物学
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微生物学
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动物科学
·
营养学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 8
·1423-1436
·
IF 4.872
Xinkun Shen,Pingping Ma,Yan Hu,Gaoqiang Xu,Kui Xu,Weizhen Chen,Qichun Ran,Liangliang Dai,Yonglin Yu,Caiyun Mu,Kaiyong Cai
Early mechanical fixation between an implant and native bone is critically important for successful orthopedic implantation, especially for hosts suffering osteoporosis with reduced bone mass. To endow a titanium-based implant with a desirable local anti-osteoporosis property for enhancing its early osseointegration, alendronate-loaded hydroxyapatite-TiO2 nanotube (TNT-HA-Aln) substrates were fabricated and systematically characterized in this study. The results of Aln/Ca2+ release and Ca2+ concentration in an osteoclast medium verified that the release of Aln was significantly accelerated along with the acidity rise caused by osteoclast differentiation. Other in vitro tests, such as CCK-8, alkaline phosphatase (ALP), mineralization, gene expression (Runx2, Osterix, ALP, Col I, OPN, OC, OPG and RANKL), protein production (OPG and RANKL) and tartrate-resistant acid phosphatase (TRAP), proved that TNT-HA-Aln substrates have great potential for improving osteoblast proliferation/differentiation and inhibiting osteoclast differentiation. Moreover, in vivo tests, such as the push-out test, micro-CT and H&E staining proved that TNT-HA-Aln implants could efficiently improve local osseointegration after implantation for 3 months. The study provides an alternative to exploiting drug–device combinations to enhance early osseointegration in osteoporosis.
研究领域:
生物医学工程
·
骨科
·
材料科学
·
药剂学
✓ 已验证
DOI
PubMed
高影响因子
Tissue Engineering and Regenerative Medicine
·
2018
·Vol. 16
·期 1
·39-50
·
IF 1.216
Zhong Yi,Jiang Ai,Sun Fei,Xiao Yuanfan,Gu Ying,Wu Lei,Zhang Yujie,Shi Hongcan
Background: Different decellularization methods can affect the integrity and the biomechanical and biocompatible properties of the tracheal matrix. Natural cross-linking with genipin can be applied to improve those properties. The goals of this study were to evaluate the effects of different decellularization methods on the properties of genipin-cross-linked decellularized tracheal matrices in rabbits. Methods: The tracheas of New Zealand rabbits were decellularized by the Triton-X 100-processed method (TPM) and the detergent-enzymatic method (DEM) and were then cross-linked with genipin. Mechanical tests, haematoxylin–eosin staining, Masson trichrome staining, Safranin O staining, DAPI staining, scanning electronic microscopy (SEM), and biocompatibility tests were used to evaluate the treatment. The bioengineered trachea and control trachea were then implanted into allogeneic rabbits for 30 days. The structural and functional analyses were performed after transplantation. Results: The biomechanical tests demonstrated that the biomechanical properties of the decellularized tracheas decreased and that genipin improved them ( p < 0.05). The histological staining results revealed that most of the mucosal epithelial cells were removed and that the decellularized trachea had lower immunogenicity than the control group. The analysis of SEM revealed that the decellularized trachea retained the micro- and ultra-structural architectures of the trachea and that the matrices cross-linked with genipin were denser. The biocompatibility evaluation and in vivo implantation experiments showed that the decellularized trachea treated with the DEM had better biocompatibility than that treated with the TPM and that immunogenicity in the cross-linked tissues was lower than that in the uncross-linked tissues ( p < 0.05). Conclusions: Compared with the trachea treated with the TPM, the rabbit trachea processed by the DEM had better biocompatibility and lower immunogenicity, and its structural and mechanical characteristics were effectively improved after the genipin treatment, which is suitable for engineering replacement tracheal tissue.
研究领域:
组织工程
·
生物医学工程
·
生物力学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RSC Advances
·
2020
·Vol. 10
·期 26
·15346-15353
·
IF 3.119
Huaqiang Mo,Chenxing Fu,Zhiye Wu,Peng Liu,Zhibo Wen,Qingqing Hong,Yanbin Cai,Gongxin Li
Vulnerable plaques of atherosclerosis (AS) are the main culprit lesion for the serious risk of acute cardiovascular disease (CVD). Therefore, developing new non-invasive methods to detect vulnerable plaques and to evaluate their stability effectively is of great value in the early diagnosis of CVD. IL-6 plays a vital role in the development and rupture of AS. In this study, IL-6-targeted superparamagnetic iron oxide nanoparticles (Anti-IL-6-USPIO) are synthesized by a chemical condensation reaction. An AS model was established by damaging rabbit abdominal aortic intima with Foley's tube in combination with a high cholesterol diet. The results confirm that Anti-IL-6-USPIO have excellent IL-6-targeting ability and usefulness in detecting vulnerable plaques in vitro and in vivo, which may provide a novel, non-invasive strategy for evaluating acute cardiovascular risk or exploiting anti-atherosclerotic drugs.
研究领域:
医学影像学
·
纳米技术
·
心脏病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2015
·Vol. 40
·期 2
·1256-1262
·
IF 3.086
Xiangyu Zhang,Kaiyue Zhao,Jun Wang,Shujie Bai,Shuqing Jiao,Jie Zhang,Lian Yu
Targeting ultrasound contrast agents (UCAs) offer further opportunity to enhance the capabilities of diagnostic ultrasound imaging and controlled drug delivery to pathological tissue. In this study, simvastatin (Sim) loaded polymeric microbubbles (MBs) as UCAs were developed and targeted toward inflamed vascular endothelium to aid in the diagnosis and treatment of atherosclerosis. The polymeric shelled MBs were used to enable localized targeting of the inflamed vascular endothelium by modifying the surface of these MBs with specific antibodies. The morphology, size distribution, chemical composition and antibody conjugation efficiency of the MBs were evaluated. The gas-filled drug delivery platform described promises faster drug release, when subjected to ultrasound, compared to the drug release without ultrasound. In vivo ultrasound contrast imaging confirmed that these polymeric MBs could provide a stable acoustic enhancement and effectively identify atherosclerotic areas of plaque within the ventral aorta of rabbits. The results demonstrated that the elaboration of MBs has the potential to exhibit excellent ultrasound contrast-enhancing capabilities and provide a sufficient amount of drug to target disease sites.
研究领域:
生物医学工程
·
药学
·
超声成像
·
药物递送系统
·
心血管疾病
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）