MOLECULES
·
2019
·Vol. 24
·期 7
·1365
·
IF 3.06
Qianyun Fu,Guoyun Li,Chen Wang,Ya Wang,Qinying Li,Jiejie Hao,Guangli Yu
Intestinal mucins constitute the major component of the mucus covering the epithelium of the gastrointestinal tract, thereby forming a barrier against microbial colonization. Rabbits are bred in large numbers worldwide, with little known about intestinalO-glycosylation despite this insight being crucial to the understanding of host-pathogen interactions. In the present study, a major mucin-type glycopeptide (RIF6) of hyla rabbit intestine was isolated and theO-glycans were extensively characterized based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) combined with bioinformatics approaches. Thirty-threeO-glycans were identified, and most of them were sulfated or sialylated glycans. It was worth noting that Neu5Gc-containing structures within sialylatedO-glycans accounted for 91%, which were extremely different from that of other species including humans, mice, chickens, etc. Sulfated glycans accounted for 58%, unique disufated and sulfated-sialylated glycans were also detected in rabbit intestinal mucin. These structural characterization reflected species diversity and may provide deeper insights into explaining the adaptability of hyla rabbit to the environment.Keywords:Hyla rabbit intestinal mucin;O-glycan;Neu5Gc;structural characterization
研究领域:
生物化学
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分子生物学
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产品关联
DOI
PubMed
高影响因子
Natural Product Communications
·
2022
·
IF 1.496
Yuyan Zhu,Lixia Zhang,Xiyuan Zhou
The prevalence of acne vulgaris is high, but the topical retinoids used as the foundation of treatment have teratogenic and photosensitivity properties. Previous studies have suggested that honokiol, a small-molecule compound extracted from Magnolia officinalis, could effectively inhibit Cutibacterium acnes (C acnes) and inflammation in vitro. However, the effect in vivo is unclear. The rabbit ear acne model that we created showed obvious comedones and hyperkeratosis. These lesions were repeatedly measured and recorded by dermatoscopy (ultraviolet light). Compared with the control group, topical 2.5% honokiol cream obviously improved the comedones and hyperkeratosis and effectively reduced sebum secretion, as shown by Oil Red O staining. The effects were equivalent to those of adapalene gel without local side effects. We added honokiol's other functions to acne treatment in addition to antiinflammation, but further studies are needed.
研究领域:
皮肤病学
·
分子生物学
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产品关联
DOI
高影响因子
INTERNATIONAL JOURNAL OF PHARMACEUTICS
·
2023
·
IF 5.8
Lu Jin,Xiumin Li,Xu Chen,Xinghao Chen,Ye Liu,Haonan Xu,Qiao Wang,Zhan Tang
Puerarin (PUE), an isoflavonoid isolated from Pueraria lobata (Willd) Ohwi root, is a β -adrenergic receptor inhibitor used in treating glaucoma. The concentration range of gellan gum was determined based on the formulation viscosity and gelling capacity. PVP-K30 and gellan gum were used as variables, with the viscosity of formulation: STF = 40: 21, the 4 h permeation rate of rabbit isolated sclera, and 2 h in vitro release rate as response values. The JMP software was used to optimize the results, presenting that gellan gum was the main factor influencing viscosity. The in vitro release and permeation rate were primarily influenced by PVP-K30. The optimal prescription was 0.45% gellan gum and 6.0% PVP-K30. The in vitro release and permeation characteristics of puerarin in situ gel (PUE-ISG) were investigated using PUE solution as a control. The dialysis bag method results indicated that the release of the solution group leveled off after 4 h, while the PUE-ISG group had been continuously releasing. However, the cumulative release rates of the two were no longer significantly different at 10 h. The cumulative permeation rates of the ISG and solution groups were not significantly different ( P > 0.05) in the rabbit isolated sclera. The apparent permeability Papp and steady-state flux Jss of PUE-ISG were 0.950 ± 0.059 cm/h and 9.504 ± 0.587 mg·(cm·h), respectively. A sensitive and stable HPLC-MS/MS analytical method for quantifying aqueous humor concentrations of PUE was validated. A microdialysis technique was successfully used in the aqueous humor pharmacokinetics study to sample aqueous humor from rabbit eye continuously. The results revealed that PUE-ISG significantly increased the drug concentration in the aqueous humor, with C max and AUC (0-t) 3.77 and 4.40 times higher than those of the solution group, respectively. T max was also significantly prolonged, indicating good prospects for clinical application. The developed PUE-ISG preparation has the characteristics of rapid drug release and sustained permeation, and increase the drug concentration in aqueous humor, with all inactive ingredients remaining within the maximum allowable limits recommended by the FDA guideline.
研究领域:
药剂学
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药物代谢动力学
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眼科
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产品关联
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
·
2020
·Vol. 56
·期 6
·3004-3015
·
IF 2.773
Zefu Wang,Zhifei He,Dong Zhang,Xiaosi Chen,Hongjun Li
Summary Frozen storage is chosen to be the preferred method in meat preservation. However, multiple freeze-thaw (F-T) cycles will occur during the transfers or distribution processes under poor cold-chain conditions. This study investigated the effects of multiple freeze-thaw (F-T) cycles on water mobility, microstructure damage, protein oxidation and lipid oxidation in rabbit meat during seven F-T cycles through 56 days. Scanning electron microscopy results indicated that the increase of F-T cycles induced the rupture of endomysia and the increase of gaps between fibres. Low-field nuclear magnetic resonance (LF-NMR) results showed that the peak area of T 21 and T 22 decreased with increasing number of repeated F-T cycles, indicating that immobile water shifted to free water, and free water mobility increased. During repeated freeze-thaw process, the peroxide value, thiobarbituric acid-reactive substances, non-haem iron content and carbonyl groups of rabbit meat gradually increased, whereas haem content, haem iron content and sulfhydryl content of rabbit meat gradually decreased. In addition, the results of Fourier transform infrared, sodium dodecyl sulphate polyacrylamide gel electrophoresis, and intrinsic fluorescence spectroscopy indirectly proved that multiple F-T cycles could cause α-helix structure disruption, protein aggregation and degradation, and tryptophan oxidation of rabbit meat myofibrillar protein (MP). Overall, multiple repeated F-T cycles changed moisture migration, damaged microstructure and promoted lipid and protein oxidation of rabbit meat.
研究领域:
食品科学
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生物化学
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DOI
高影响因子
Water
·
2023
·Vol. 15
·期 11
·2015
·
IF 3.4
Feng Ouyang,Yujie Liu,Jiao Chen,Chenghan Tang,Aojie Wang,Yixin Lu,Yiping Yuan
Using rabbit manure as raw material, three distinct types of rabbit manure biochar (RBC400, RBC500, and RBC600) were prepared via pyrolysis at 400 °C, 500 °C, and 600 °C, respectively. The effects of pyrolysis temperature on the physicochemical properties of biochar were examined by scanning electron microscopy, Brunauer–Emmett–Teller analysis, Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and X-ray diffraction techniques. Rabbit manure biochar was used to activate permonosulfate (PMS) to degrade rhodamine B (RhB). The findings revealed that RBC600 prepared at 600 °C showed the strongest catalytic activity due to its abundant pores and pore structure, high graphitization, and high carbonization degree. Under optimal reaction conditions (0.4 g/L PMS and 0.6 g/L RBC600), the degradation rate of 50 mg/L RhB reached 93.38% within 60 min. RBC600 can be effectively recovered, and effective catalytic activity can be sustained after five cycles. The quenching and EPR experiments showed that both free-radical (SO4−and ·OH) and non-free-radical (1O2) pathways contributed to the degradation of RhB, in which1O2performed a dominant role. In conclusion, the new PMS activator prepared in this study not only realizes the “waste to waste” use of manure waste but also provides technical support for the efficient resource-based treatment of organic wastewater.Keywords:rabbit manure;biochar;permonosulfate;advanced oxidation;rhodamine B
研究领域:
环境科学
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材料科学
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产品关联
DOI
高影响因子
MOLECULAR PHARMACEUTICS
·
2024
·
IF 4.5
Teng Chen,Zuhua Wang,Xingchu Gong,Jiaojiao Zhang,Ning Zhang,Jing Yang,Yue Zhu,Ying Zhou
Hypertrophic scars (HS) still remain an urgent challenge in the medical community. Traditional Chinese medicine (TCM) has unique advantages in the treatment of HS. However, due to the natural barrier of the skin, it is difficult for the natural active components of TCM to more effectively penetrate the skin and exert therapeutic effects. Therefore, the development of an efficient drug delivery system to facilitate enhanced transdermal absorption of TCM becomes imperative for its clinical application. In this study, we designed a compound Salvia miltiorrhiza–Blumea balsamifera nanoemulsion gel (CSB-NEG) and investigated its therapeutic effects on rabbit HS models. The prescription of CSB-NEG was optimized by single-factor, pseudoternary phase diagram, and central composite design experiments. The results showed that the average particle size and PDI of the optimized CSB-NE were 46.0 ± 0.2 nm and 0.222 ± 0.004, respectively, and the encapsulation efficiency of total phenolic acid was 93.37 ± 2.56%. CSB-NEG demonstrated excellent stability and skin permeation in vitro and displayed a significantly enhanced ability to inhibit scar formation compared to the CSB physical mixture in vivo. After 3 weeks of CSB-NEG treatment, the scar appeared to be flat, pink, and flexible. Furthermore, this treatment also resulted in a decrease in the levels of the collagen I/III ratio and TGF-β1 and Smad2 proteins while simultaneously promoting the growth and remodeling of microvessels. These findings suggest that CSB-NEG has the potential to effectively address the barrier properties of the skin and provide therapeutic benefits for HS, offering a new perspective for the prevention and treatment of HS.
研究领域:
药剂学
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皮肤科学
·
传统中药研究
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF ULTRASOUND IN MEDICINE
·
2024
·
IF 2.1
Xinyu Zhong,Xinghao Zhang,Yuting Cao,Ping Zhou
Objectives This study aimed to explore the interactions between microbubbles and microwave ablation (MWA). Methods The study employed custom-made phantoms (in vitro) and white New Zealand rabbits (in vivo). MWA was performed with or without microbubbles in the phantoms (2 × 10 5 particles mL −1 ) and rabbit livers (intravenous injection of 0.05 mL/kg SonoVue). During the MWA, K-type thermocouple probes were used to monitor the MWA-induced temperature increase. Contrast-enhanced ultrasound imaging (CEUS) was used to monitor and analyze the microbubbles signal intensity. After MWA, the ablation-zone volumes were evaluated and compared between the groups with and without microbubbles. Results In both the phantom models and rabbits, microbubbles showed no significant influence on MWA, including the ablation range and MWA-induced temperature increase. In phantoms and rabbit livers filled with microbubbles, MWA caused the formation of a gradually expanding microbubble-defect region over the ablation time. An increase in the temperature caused microbubble destruction. Conclusions Microbubbles had no significant influence on MWA. However, MWA induced the destruction of microbubbles in a temperature-dependent manner. Thus, the poor thermotolerance of microbubbles is a non-negligible barrier when using CEUS to monitor the ablation range during MWA in real-time.
研究领域:
医学影像学
·
肿瘤学
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DOI
PubMed
高影响因子
Food Bioscience
·
2024
·
IF 4.8
Jing Zhang,Jingyi Yang,Xudong Han,Yuyao Ren,Yan Liu,Bohao Zhao,Xinsheng Wu,Yang Chen
Rabbit meat is recognized for its nutritional benefits and affordability. However, research into the primary factors influencing its flavor, especially among various rabbit breeds, remains insufficient. This study conducted a comprehensive comparison of meat quality, metabolomic, and transcriptomic profiles between the longissimus dorsi muscles of Fujian yellow rabbits (FJY) and New Zealand white rabbits (NZW), which exhibit significant variations in body size and growth rate. A total of 68 samples, with 34 from each breed, were analyzed. Results indicated that FJY rabbits had lower body weights but higher dressing percentages than NZW rabbits. FJY meat was higher brightness (L*) and yellowness (b*), lower redness (a*), along with significantly reduced cooking losses. In terms of proximate composition, FJY meat exhibited higher protein and collagen levels but significantly less fat and moisture than NZW meat. Additionally, FJY meat contained higher levels of myristic acid, palmitoleic acid, cis-11-eicosapentaenoic acid, α-linolenic acid, and a higher MUFA/FA ratio. Metabolomic analysis using HPLC-MS/MS identified 231 differential metabolites, including amino acids and their derivatives, organic acids, and heterocyclic compounds. Transcriptomic analysis via RNA-seq revealed 211 differentially expressed genes potentially involved in meat quality regulation. Integrated analyses identified 70 KEGG pathways associated with pivotal genes, such as CPT2, PAK1, SLC7A7, and TAS1R2 , which exhibited correlations with differential metabolites like cholesterol, benzoic acid, putrescine, and N-acetylcysteine, suggesting a synergistic effect on meat quality. Overall, these findings provide valuable insights into the distinct meat quality characteristics that underpin the improved quality and flavor of rabbit meat.
研究领域:
动物科学
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分子生物学
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食品科学
·
营养学
✓ 已验证
DOI
高影响因子
Neurocritical Care
·
2021
·Vol. 35
·期 2
·367-378
·
IF 3.21
Li Yinghui,Ren Siying,Wang Likun,Mao Yuanhong,Wu Guofeng,Li Qi,Tang Zhouping
Background Blend sign on initial computed tomography (CT) is associated with poor outcome in patients with intracerebral hemorrhage (ICH). However, the mechanisms underlying the blend sign formation are poorly understood. The present study aimed to explore the possible mechanism of the CT blend sign in patients with ICH. Methods Seventy healthy rabbits were selected to prepare an ICH model. The animals were assigned to a whole blood group + whole blood group (ww group, 50 rabbits), a whole blood + plasma group (wp group, 10 rabbits) or a whole blood + serum group (ws group, 10 rabbits). The animals of the ww group were allocated to five subgroups based on the interval between the first infusion of blood and the second one. The subgroups included ww 1 h group (with an interval of 1 h), ww 2 h group, ww 3 h group, ww 4 h group and ww 5 h group. The rabbits from each group received first infusion of 0.3 mL of whole blood into the basal ganglia area to form a hematoma. Then, they received a second infusion of the same amount of whole blood, plasma or serum into the brain to form another hematoma adjacent to the first one. Results A hematoma with two densities on brain CT could be formed in each group after a second infusion of blood into the brain. A significant difference in CT attenuation values was observed between the hyperattenuation and the hypoattenuation in all the groups. However, only the morphological features of the hematoma in the ww group was in accordance with the CT blend sign observed in humans. The CT attenuation values in the hypodensity area of the ww 4 h group or the ww 5 h group were decreased compared with the ww 1 h group to the ww 3 h group. Conclusions The CT blend sign observed in humans might be composed of two parts of blood with different ages. The hypodense area might be blood with older age and the hyperdense area might be new bleeding.
研究领域:
医学影像学
·
神经病学
✓ 已验证
DOI
高影响因子
Scientific Reports
·
2024
·Vol. 14
·期 1
·1-9
·
IF 3.8
Shi Qianwen,Wang Lele,Li Wenyue,Feng Yongming,Wei Zhiwen,Yun Keming
Nonoxidative ethanol metabolites have been reported as ethanol biomarkers in clinical and forensic cases. However, their forensic toxicokinetics are still unclear. The study aimed to simultaneously research the postmortem distribution and dynamic distribution of ethanol and its nonoxidative metabolites in 62 poisoned rabbits. Firstly, 32 rabbits were randomly divided into three groups and sacrificed after three doses ethanol, and their organs were collected to study the postmortem distribution of ethanol and its nonoxidative metabolites. The results showed that the distribution trends of ethanol and its nonoxidative metabolites were basically same between different groups, and ethanol could be better detected by collecting body fluids, while the nonoxidative metabolites could be better detected by collecting the heart, liver, kidney and testis. Secondly, 30 rabbits after 1/2LD 50 ethanol intragastric administration were sacrificed at 10 time points, and their organs were collected to study the dynamic distribution of ethanol and its nonoxidative metabolites. The results showed the concentrations of ethanol and its nonoxidative metabolites increased with time and then decreased. All analytes could be detected in all samples within 8 h, with the exception of ethyl oleate, which kept high level in muscle and vitreous humor, and could be detected within 4 h.
研究领域:
法医学
·
毒理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
RENEWABLE ENERGY
·
2024
·
IF 9
Xiangjun Guo,Yun Huang,Yunheng Tang,Ao Xia,Xianqing Zhu,Xun Zhu,Qiang Liao
Mixed bacterial syngas fermentation is a promising carbon-neutral method for producing acetic acid and ethanol. However, the bacterial community and metabolic pathways were different in the growth and ethanol production stages, whereas the activity of the bacterial community greatly depended on the pH of the culture. In this study, the effect of pH on syngas fermentation performance and microbial community composition under continuous gas supply was investigated. The mixed bacteria grew fastest at a pH of 7, while a constant pH of 6 led to the highest ethanol concentration of 5.15 g L −1 , which was 160 % higher than that at pH 7. Based on this, a two-step pH regulation method was proposed in this study to improve the ethanol production. Initially, the culture pH was maintained at 7 to create optimal growth conditions. Once bacterial density stabilized, it was then changed to a pH 6 to promote ethanol production. By doing this, compared with the control group, the maximum ethanol concentration (5.85 g L −1 ) increased by 148.9 %, the ethanol production rate (2.41 g L −1 d −1 ) increased by more than 8 times. This two-step pH regulation method ensured a high bacterial density and effectively stimulated ethanol production during syngas fermentation.
研究领域:
微生物学
·
生物技术
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DOI
高影响因子
BIOLOGICAL TRACE ELEMENT RESEARCH
·
2021
·Vol. 199
·期 12
·4614-4623
·
IF 3.738
Li Fan,Liu Lei,Chen Xiaoyang,Zhang Bin,Li Fuchang
Copper is often used as a growth promoter, at the same time copper is one of the most important essential trace elements for fur animals, especially Rex rabbits. However, too much copper added to the diet may harm animal health, and copper excreted in feces can pollute the environment. In this study, 3-month-old Rex rabbits were randomly divided into four groups and fed a basal diet containing 0, 30, 60, or 120 mg/kg Cu for 5 weeks. The diet supplemented with 30 mg/kg Cu significantly increased ( P < 0.05) the average daily feed intake (ADFI) and the average daily gain (ADG) and also the activity of serum Cu–Zn (zinc) superoxide dismutase and the digestibility of ether extract. Supplemental Cu up to 120 mg/kg did not significantly adversely affect the Zn metabolism of growing Rex rabbits. Overall, the data in this study indicate that 30 mg/kg is the optimal level of Cu supplementation in the diet of growing Rex rabbits. The results will provide a reference to improve the breeding of Rex rabbits and possibly other animals. In follow-up studies, the amount of copper in the diet should be reduced as much as possible from the baseline of 30 mg/kg copper.
研究领域:
动物营养学
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环境科学
·
兽医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）