JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Zhan Li,Zhen Zhang,Yifan Gao,Kongyu Ge,Hongyu Yi,Hongjun Ji,Mingyu Li,Huanhuan Feng
Hypothesis The fabrication and optical performance of structural colors are influenced by microstructure geometry and surface interactions. Total internal reflection (TIR)-based structural colors, formed by microspherical crown structures on hydrophobic substrates, are expected to exhibit tunable optical properties such as single-sided visibility and angle dependence. By precisely controlling droplet volume and substrate wettability, it should be possible to achieve customizable anti-counterfeiting features and color blindness detection applications. Experiments Polyacrylic acid (PAA)-based inks were formulated with optimized molecular weight, solid content, and solvent composition to enable stable precision printing. Microspherical crown structures were printed on hydrophobic substrates under varying droplet volumes and contact angles. The printed patterns were characterized using scanning electron microscopy (SEM) and optical spectroscopy to analyze their morphology and color tunability. Environmental stability was tested under UV exposure, high temperatures, and freezing conditions. Findings The printed TIR structural colors displayed single-sided visibility, angle-dependent reflectance, and high color saturation. Controlled variations in microstructure size and contact angle enabled fine-tuning of the optical response. The technique successfully produced transparent anti-counterfeiting labels and a single-sample diagnostic tool for distinguishing different types of color blindness. The structural colors remained stable under environmental stress, demonstrating their potential for practical applications in security features and biomedical diagnostics.
研究领域:
材料科学
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光学
·
生物医学工程
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DOI
PubMed
高影响因子
Journal of Physical Chemistry C
·
2011
·
IF 4.524
Zhuo Ao,Guangming Liu,Guangzhao Zhang
We have investigated the ion specificity at low salt concentrations in view of surface force by use of total internal reflection microscopy (TIRM). The polystyrene (PS) particle−glass slide interaction potential energies in different salt solutions have been measured as a function of the concentration of each salt, and the separation distance (hm) corresponding to the minimum potential energy as well as the Debye length (κ−1) are obtained from the potential profiles. In the case of cation specificity, a V-shaped cation series is observed in terms of hm, which is attributed to the difference in the direct interactions between the cations and the sulfate groups on the particle surface on the basis of the law of matching water affinities. The cation specificity of κ−1 indicates that the effective double layer thickness increases with the effective size of hydrated counterions. In the case of anion specificity, the anion sequence in terms of hm also exhibits a V-shaped series due to the accumulation or exclusion of anions near the particle surface. Moreover, the anion specificity of κ−1 is more complex than the cation specificity because the distribution of anions in the electrical double layer is influenced by both the anion−surface and anion−cation interactions.
研究领域:
物理化学
·
界面科学
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Xinhong Ye,Ruoxin Wang,Hongqi Chen,Yufeng Wen,Lun Wang
With the rapid development of science and technology, single particle imaging technology has been greatly improved, and many more sensitive optical microscopes have been rapidly developed. Herein, we propose a single particle imaging method for quantitative image analysis and cell imaging of peroxynitrite based on upconversion luminescence total internal reflection imaging platform. Cyanine-dye-assembled composite upconversion nanoparticles (UCNPs) were prepared and used as luminescent probe. For the quantitative image analysis method, the numbers of photons are obtained by calculating the average brightness of 550 frames of sequence images based on the Brownian motion of the luminescent nanoparticles. Compared to the fluorescence spectrometer method, the quantitative image analysis method for sensing peroxynitrite has many advantages such as less sample consumption (≤10 μL), fast detection time (~0.1 s) and low detection limit (0.067 μM). Due to the unique advantages of UCNPs luminescent materials, such as low biotoxicity, near-infrared emission and high penetration depth, the luminescent materials are also applied to image and semiquantitatively detect of exogenous peroxynitrite in living cells based on the single particle imaging platform. This work not only greatly improves the efficiency in vitro study, but also provides a simple, sensitive and convenient semiquantitative detection method for single particle imaging of living cells.
研究领域:
生物分析化学
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医学影像学
·
纳米生物学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2018
·
IF 6.042
Pengcheng Hu,Bolin Li,Chen Bai,Xu Li,Xiaolin Lu
With the rapid development of materials science, on-demand techniques are highly needed with the capability to characterize materials in the micrometer and nanometer scales. In this work, we show that, by employing a prism geometry, total internal reflection sum frequency generation (SFG) vibrational spectroscopy allows for characterizing mesoscopic materials with irregular or complex surfaces. Four representative examples were presented. First, we reveal that mechanical grinding can subtly alter the surface molecular structures of original materials. Second, spin coating can substantially change the surface molecular structures of particle samples. Third, surface restructuring of carboxylated multiwalled carbon nanotubes can happen in response to the surrounding environment. Fourth, surface adsorption and desorption dynamics of toluene on activated charcoal can be traced. Such experiments demonstrate that there are still a broad range of research fields ahead SFG can be directed to, where materials in mesoscopic scales with irregular or complex surfaces can be studied.
研究领域:
材料科学
·
表面化学
·
光谱学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Hao Zhu,Zheng Cai,Zixuan Chen,Yun Chen,Jun-Jie Zhu
Since the formation of a DNA duplex plays a vital role in gene expression and regulation across all kinds of organisms, quantifying the interaction during DNA hybridization is essential for understanding various biological processes in living systems on the molecular scale of nucleic acids. Here, we developed a label-free method to measure the binding kinetics and affinity of DNA hybridization with an electro-optical modulation on the individual gold nanorods based on a total internal reflection dark-field imaging microscopy. Under the electrochemical modulation, a Fourier transform filter was utilized to extract the optical scattering of the nanorods, which varies with the DNA binding due to their impedance change. We validated the imaging principle and established an analytical model to monitor the optical response during the DNA hybridization. Using the presented platform, we measured the binding kinetics and affinities of different DNA pairs and demonstrated its capability to distinguish the DNA hybridization with only single-base mismatch, which may provide guidance to explore the etiology and pathogenesis of diseases associated with single point gene mutations. Furthermore, the method allows for simultaneous imaging of multiple nanoparticles, thus enabling a high-throughput detection and opening possibilities for the study of the interfacial heterogeneity.
研究领域:
生物物理学
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分子生物学
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纳米技术
·
遗传学
✓ 已验证
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2017
·
IF 5.401
Wen-Shuai Jiang,Wei Xin,Shuang Xun,Shao-Nan Chen,Xiao-Guang Gao,Zhi-Bo Liu,Jian-Guo Tian
A sensitive and selective optical biosensor based on reduced graphene oxide (RGO), which uses the polarization-dependent absorption effect of graphene under total internal reflection, is reported for determination of rabbit IgG. RGO sheets with the thickness of about 8.1 nm are fabricated by high temperature reduction and used as a sensing film, because of its strong polarization dependent absorption. This RGO-based optical sensor shows a satisfactory response to rabbit IgG with a minimum concentration of 0.0625 μg/ml. As a contrast, commercial SPR apparatus is used to investigate rabbit IgG with a minimum concentration of 0.3125 μg/ml. Moreover, with antigen–antibody binding, this sensor can also achieve label-free and real-time detection. Taking into account these factors, the RGO-based optical sensor may be a potential candidate for biosensing.
研究领域:
材料科学
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生物传感器
·
光学
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2022
·
IF 9.221
Chongqi Zhou,Yang Xu,Xiaonan Zhang,Zhangyan Li,Tian Guan,Yonghong He,Yanhong Ji
A modified weak measurement scheme, rotatory biased weak measurement, is proposed to significantly improve the sensitivity and resolution of the total internal reflection sensor. This method introduces an additional phase in the post-selected procedure and generates an extinction point in the spectrum distribution. The biased post-selection makes smaller coupling strength available, which leads to an enhancement of phase sensitivity and refractive index sensitivity. In rotatory biased weak measurement, an enhanced refractive index sensitivity of 13605 nm/RIU is achieved compared to 1644 nm/RIU in standard weak measurement. The performance of total internal reflection sensors with different sensitivity is analyzed, and it is shown the optimal refractive index resolution of sensors increases with sensitivity. An optimal refractive index resolution of 4 × 1 0 − 7 RIU on a total reflection structure is demonstrated, which is far beyond the state-of-the-art resolution of 3 × 1 0 − 6 RIU of total internal reflection sensors. The rabbit anti-mouse IgG and mouse IgG binding reaction experiments demonstrate that our system has a high response to the concentration of IgG in a wide range and the limit of detection is 15 ng/mL. This work provides a total internal reflection sensor with the advantages of simple structure, low cost, and high resolution at the same time.
研究领域:
光学
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传感器
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生物物理学
✓ 已验证
产品关联
DOI
高影响因子
APPLIED OPTICS
·
2022
·Vol. 61
·期 34
·10065-10071
·
IF 1.905
Jing-Ru Zhao,Jing-Ru Zhao,Zhen-Jie Wu,Gui-Qiang Wang,Chun-Nan Wang,Bo-Fu Deng,Shu-Qing Sun
A total internal reflection system based on the weak value amplification principle is set up for the precise measurement of the thickness of an ultra-thin film. In this system, the film thickness is derived from the change of the double-peak pointer caused by the effective refractive index of the film, which is correlated to its thickness. The sensitivity and resolution of this system reached 2727.21 nm/RIU and ${7.2} \times {{10}^{- 6}}\;{\rm RIU}$, respectively, determined by using a sodium chloride solution with a refractive index of 1.331911. The growth process of TA/Fe(III) assembled films with thicknesses in the few nanometers range is monitored using the as-set-up system, and the experimental results are consistent with a theoretical calculation based on the Maxwell Garnett effective medium. Additionally, we theoretically calculated the detection limit for the thickness measurement of the film as 22 pm. We clearly provide a potential method for the precise measurement of the thickness of an ultra-thin film.
研究领域:
光学
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材料科学
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物理学
✓ 已验证
DOI
PubMed
高影响因子
MICROCHIMICA ACTA
·
2024
·Vol. 191
·期 8
·1-12
·
IF 5.3
He Qian,Chen Qun,Lian Lijin,Qu Jiuxin,Yuan Xi,Wang Chuhui,Xu Lidan,Wei Jiazhang,Zeng Shaoling,Yu Dongmei,Dong Yuhan,Zhang Yongbing,Deng Lin,Du Ke,Zhang Canyang,Pandey Vijay,Gul Ijaz,Qin Peiwu
The CRISPR/Cas13 nucleases have been widely documented for nucleic acid detection. Understanding the intricacies of CRISPR/Cas13’s reaction components is pivotal for harnessing its full potential for biosensing applications. Herein, we report on the influence of CRISPR/Cas13a reaction components on its trans-cleavage activity and the development of an on-chip total internal reflection fluorescence microscopy (TIRFM)-powered RNA sensing system. We used SARS-CoV-2 synthetic RNA and pseudovirus as a model system. Our results show that optimizing Mg 2+ concentration, reporter length, and crRNA combination significantly improves the detection sensitivity. Under optimized conditions, we detected 100 fM unamplified SARS-CoV-2 synthetic RNA using a microtiter plate reader. To further improve sensitivity and provide a new amplification-free RNA sensing toolbox, we developed a TIRFM-based amplification-free RNA sensing system. We were able to detect RNA down to 100 aM. Furthermore, the TIRM-based detection system developed in this study is 1000-fold more sensitive than the off-coverslip assay. The possible clinical applicability of the system was demonstrated by detecting SARS-CoV-2 pseudovirus RNA. Our proposed sensing system has the potential to detect any target RNA with slight modifications to the existing setup, providing a universal RNA detection platform. Graphical Abstract
CAS / MDL:
研究领域:
生物传感
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分子生物学
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纳米技术
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病毒学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Frontiers in Plant Science
·
2017
·
IF 4.291
Song Song,Chang Jian,Ma Chongjun,Tan Yan-Wen
Plant-hormone-initiated signaling pathways are extremely vital for plant growth, differentiation, development, and adaptation to environmental stresses. Hormonal perception by receptors induces downstream signal transduction mechanisms that lead to plant responses. However, conventional techniques—such as genetics, biochemistry, and physiology methods—that are applied to elucidate these signaling pathways can only provide qualitative or ensemble-averaged quantitative results, and the intrinsic molecular mechanisms remain unclear. The present study developed novel methodologies based on in vitro single-molecule fluorescence assays to elucidate the complete and detailed mechanisms of plant hormone signal transduction pathways. The proposed methods are based on multicolor total internal reflection fluorescence microscopy and a flow cell model for gas environment control. The methods validate the effectiveness of single-molecule approaches for the extraction of abundant information, including oligomerization, specific gas dependence, and the interaction kinetics of different components.
研究领域:
植物生物学
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分子生物学
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生物物理学
·
细胞信号传导
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF FLUORESCENCE
·
2015
·Vol. 25
·期 5
·1267-1277
·
IF 1.927
Huang Weidong,Wang Yue,Wang Zhimin
Single-molecule detection (SMD) and single-molecule fluorescence resonance energy transfer (smFRET) were conducted using Cy3- and Cy5-labeled single-strand DNAs (ssDNAs) either immobilized on substrates or encapsulated in microdroplets. High-quality fluorescent images were obtained using a total internal reflection fluorescence microscope (TIRFM). In the substrate system, deposition of a low concentration of fluorescence molecules on substrates through electrostatic adsorption showed that most of the fluorescence spots were single molecules, and the mean value of signal to noise ratio (S/N) reached 6.9 ± 0.34. smFRET analysis was conducted through immobilization of donor- and acceptor-labeled oligonucleotides on substrates. In the droplet system, fluorophor-labeled oligonucleotides were injected into T-type microfluidics. Single and double fluorophor-labeled DNA molecules encapsulated in droplets were detected, the FRET efficiency and inter-dye distance of a single donor-acceptor pair were measured accurately. smFRET was conducted detailedly in the tortuous channel for the first time.
研究领域:
生物化学
·
分子生物学
·
生物物理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Yating Zhou,Xianzhu You,Wenying Liu,Wei Yang,Xuru Jin,Xiaopeng Pei,Sheng Xiang,Hua Zhou,Zhiyong Liao,Ying Tan
Structural colors generated via total internal reflection (TIR) using nanostructure-free micro-concave shapes have garnered increasing attention. However, the application of large micro-concave structures for structural coloration remains limited. Herein, a flexibly tunable structural color film fabricated by casting polydimethylsiloxane (PDMS) on an array of large poly(glycidyl methacrylate) (PGMA) bowl-shaped particles is reported. The resultant film exhibits tunable red to green structural colors with changing observation angles. Moreover, the color can be further tailored by altering the shape of the film itself. The incorporation of the PDMS layer not only facilitates a shift in the locus of TIR from the bottom surface to the top concave surface of the particles, thereby enabling the generation of structural color, but also confers enhanced flexibility to the film. Further decoration with silver nanoparticles imparts antimicrobial properties, yielding a novel antimicrobial coating material with structural colors. The simple and cost-effective strategy for the production of structural color films provides potential applications in antimicrobial coatings, enabling accessible and customizable structural coloration using big-size micro-concave particles.
研究领域:
材料科学
·
光学
·
纳米技术
·
涂层与薄膜
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）