Small
·
2018
·Vol. 14
·期 39
·1802107
·
IF 9.598
Youchuang Chao,Sze Yi Mak,Shakurur Rahman,Shipei Zhu,Ho Cheung Shum
Droplets containing ternary mixtures can spontaneously phase-separate into high-order structures upon a change in composition, which provides an alternative strategy to form multiphase droplets. However, existing strategies always involve nonaqueous solvents that limit the potential applications of the resulting multiple droplets, such as encapsulation of biomolecules. Here, a robust approach to achieve high-order emulsion drops with an all-aqueous nature from two aqueous phases by osmosis-induced phase separation on a microfluidic platform is presented. This technique is enabled by the existence of an interface of the two aqueous phases and phase separation caused by an osmolality difference between the two phases. The complexity of emulsion drops induced by phase separation could be controlled by varying the initial concentration of solutes and is systematically illustrated in a state diagram. In particular, this technique is utilized to successfully achieve high-order all-aqueous droplets in a different aqueous two-phase system. The proposed method is simple since it only requires two initial aqueous solutions for generating multilayered, organic-solvent-free all-aqueous emulsion drops, and thus these multiphase emulsion drops can be further tailored to serve as highly biocompatible material templates.
研究领域:
生物技术
·
材料科学
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产品关联
DOI
PubMed
高影响因子
FIBERS AND POLYMERS
·
2021
·Vol. 22
·期 11
·2972-2978
·
IF 2.153
You Haining,Zhang Qiang,Yan Shuqin,You Renchuan
Chondroitin sulfate (CS) is a naturally derived bioactive macromolecule and has been used to improve the performance of silk fibroin (SF) biomaterials for soft tissue regeneration. However, it is still a challenge to use a chemical-free method to prepare water-insoluble SF/CS scaffolds. Here, a novel all-aqueous process was developed to prepare SF/CS scaffolds with controlled properties. Freezing-annealing treatment was used to induce silk I crystallization of SF to entrap CS macromolecules. It was found that the addition of CS did not prevent silk I crystallization of SF, even with a ratio as high as 10 % addition ratio. With increasing CS content, the water solubility of the scaffolds increased due to insufficient silk I crystals. The physicochemical properties of the scaffolds were evaluated, and the results showed that the water binding capacity and mechanical properties of the SF scaffolds can be effectively regulated by adding only less than 5 % CS content. This study provides a green strategy, including all-aqueous, low temperature, and without the use of toxic chemicals or solvents, to prepare bioactive SF/CS scaffolds for use in tissue engineering.
研究领域:
生物材料学
·
再生医学
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产品关联
DOI
高影响因子
MICROCHEMICAL JOURNAL
·
2021
·
IF 4.821
Tong Chen,Liang Xu,Guangsan Song,Yuan Li,Hong Xu,Hongbin Zhou,Zhen Xiao,Ping Li
Au nanoparticles (Au NPs)-decorated SO 3 H-cofunctionalized porous silica particles was prepared as a novel mixed-mode stationary phase and used in Per aqueous liquid chromatography (PALC) mode. The obtained stationary phase was characterized by elemental analysis, FT-IR spectra, and transmission electron microscopy. To evaluate the retention behavior, the effect of a variety of chromatographic factors on the retention of different types of solutes was investigated. The obtained column exhibited a hydrophobic interaction/cation-exchange mixed-mode retention mechanism, and a typical PALC characteristic was observed in water-rich mobile phases. When the particle size of Au NPs was 10–20 nm, the analytes had a sharp peak shape and the column pressure was also moderate. Compared with the hydrophilic interaction liquid chromatography (HILIC) and C 18 column, some hydrophilic compounds such as six monosaccharides were baseline separated with the enhanced resolution of the obtained column under the PALC mode. Moreover, the efficiency of the Au-S/SO 3 H-SiO 2 -2 column was significantly higher than that of the HILIC column. PALC mode was a green annlysis method and suitable for the replacement of hydrophilic interaction liquid chromatography.
研究领域:
分析化学
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2024
·
IF 9.4
Tao Zhou,Zhou Liu,Xudong Ma,Chaofeng Cen,Zhangwei Huang,Yi Lu,Tiantian Kong,Cheng Qi
The essence of compartmentalization in cells is the inspiration behind the engineering of synthetic counterparts, which has emerged as a significant engineering theme. Here, we report the formation of ultra-stable water-in-water (W/W) emulsion droplets. These W/W droplets demonstrate previously unattained stability across a broad pH spectrum and exhibit resilience at temperatures up to 80℃, overcoming the challenge of insufficient robustness in dispersed droplets of aqueous two-phase systems (ATPS). The exceptional robustness is attributed to the strong anchoring of micelle-like casein colloidal particles at the PEO/DEX interface, which maintains stability under varying environmental conditions. The increased surface hydrophobicity of these particles at high temperatures contributes to the formation of thermally-stable droplets, enduring temperatures as high as 80℃. Furthermore, our study illustrates the adaptable affinity of micelle-like casein colloidal particles towards the PEO/DEX-rich phase, enabling the formation of stable DEX-in-PEO emulsions at lower pH levels, and PEO-in-DEX emulsions as the pH rises above the isoelectric point. The robust nature of these W/W emulsions unlocks new possibilities for exploring various biochemical reactions within synthetic subcellular modules and lays a solid foundation for the development of novel biomimetic materials.
研究领域:
材料科学
·
生物技术
·
化学工程
·
胶体科学
·
生物材料
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）