COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING
·
2024
·
IF 1.6
Yuanxia Yang,Miaoting Yang,Lijuan Wang,Liufang He,Tao Shi
Background: The establishment and validation of methods for testing biological samples are crucial steps in pharmacokinetic studies. Currently, several methodological reports have been published on the detection of rapamycin plasma concentrations. Objective: The objective of this study was to explore an effective method for detecting rapamycin in rat whole blood biological samples. Method: In this study, we designed a rapid, sensitive, and specific liquid chromatograph-mass spectrometer/mass spectrometer (LC-MS/MS) methodology for detecting rapamycin in rat whole blood biological samples. We comprehensively validated the specificity, linear range, lower limit of quantification (LLOQ), precision, accuracy, recovery, and stability of this method. Results: The findings of this study confirmed the successful implementation of LC-MS/MS for the detection of rapamycin, demonstrating its sensitivity, specificity, and reliability in quantitative analysis. This method ensures the accuracy and reliability of subsequent study data through our validated LC-MS/MS approach. Conclusion: The results demonstrated the successful implementation of an LC-MS/MS method for sensitive, specific, and reliable quantitative analysis of rapamycin in rat whole blood samples. This method ensures the accuracy and reliability of subsequent study data. Significance: The importance of this study lies in the successful establishment of a rapid, sensitive, and specific LC-MS/MS method for detecting rapamycin concentration in rat whole blood, ensuring the accuracy and reliability of subsequent research data. This provides a crucial tool and foundation for further understanding the metabolism and pharmacological effects of rapamycin in vivo, aiding in the advancement of drug research and clinical applications in related fields.
研究领域:
药代动力学
·
药物分析
·
生物样本检测
✓ 已验证
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Haijing Tian,Ruo Wu,Qi Lv,Jie Shi,Xiaoqin Guo,Duo Li,Chunli Liu,Qi Li,Weijia Zheng,Haojun Fan,Xuexin Duan,Qiannan Xue
Blood potassium and creatinine levels are critical indicators for managing chronic kidney disease (CKD). In response to the need for home monitoring and emergency care, point-of-care diagnostic technologies based on microsystems have emerged, nevertheless they remain limited in portability and speed for direct in whole blood samples measurement. To enable direct blood detection of potassium and creatinine for effective CKD management, this study presents a novel on-chip self-driven dual-channel optical microsensor designed for real-time monitoring of potassium and creatinine levels in whole blood. Utilizing 3D printing technology to design and fabricate microstructures, and integrating microchannels with a filtration layer, this microsensor requires only 10 μL of blood for single-pass sample filtration and dual-parameter detection. A novel passive blood sample pumping method is proposed, utilizing internal and external pressure differentials along with internal hydrophilic properties to facilitate sample flow into the detection chambers. Light-emitting diodes (LEDs) and photodiodes, positioned on either side of the detection chamber, are employed to measure the concentrations of blood indicators. The detection results can be instantly handheld read and uploaded to the cloud via intelligent terminal, enabling seamless follow-up diagnostics and treatment. Through multiple rounds of testing on animal serum samples, whole blood samples, and real porcine blood samples, this microsensor demonstrated excellent linearity and accuracy. This innovative microsensor, designed for direct testing from a minimal volume of whole blood, holds significant potential for improving CKD management, offering a portable, reliable alternative for preoperative diagnostics and home-based chronic disease monitoring.
研究领域:
医学工程
·
生物医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemElectroChem
·
2017
·Vol. 4
·期 3
·472-475
·
IF 4.136
Fanyu Meng,Weiguo Liang,Haixuan Sun,Ligang Wu,Xiao Gong,Peng Miao
Graphical Peptide–heparin composites : A simple electrochemical approach for the determination of heparin level in human whole-blood is developed based on specific peptide recognition (see picture). The first peptide-based electrochemical biosensor for the measurement of the anti-coagulant heparin using electrochemical impedance spectroscopy is reported. The peptide immobilized on the electrode interface specially captures heparin with high affinity. The composites formed on the electrode can thus effectively repel electrochemical species, leading to an increase in interfacial charge transfer resistance. After analyzing the electrochemical signals, a linear detection range from 0.05 to 10.0 μg/mL is obtained. The detection limit is calculated to be 0.01 μg/mL, which is extremely low with such a simple sensing mechanism. Moreover, the proposed method has potential application for sensitive and separation-free detection of heparin in whole-blood samples.
研究领域:
生物化学
·
医学检测
·
电化学
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Hui Dong,Yanli Zhou,Yuanqiang Hao,Le Zhao,Shuo Sun,Yintang Zhang,Baoxian Ye,Maotian Xu
Oxidative stress plays an important role in the pathogenesis of many diseases, while the exact mechanism that hydrogen peroxide (H 2 O 2 ) as one of the most abundant reactive oxygen species (ROS) exerts its influence on oxidative stress remains unclear. We developed a novel turn-on ratiometric electrochemical sensor for the detection of H 2 O 2 in blood samples. The electrochemical probe 5-(1,2-dithiolan-3-yl)-N-(4-(4,4,5,5-tetramethyl-1,3,2-dioxaborolan-2-yl)phenyl)pent-anamide (BA) was designed and synthesized for the selective detection of H 2 O 2 via a one-step amide reaction. Meanwhile, Nile Blue A (NB) was optimized as an internal reference molecule, thus enabling accurate quantification of H 2 O 2 in a complex environment. BA and NB were then co-assembled onto a carbon fiber microelectrode (CFME) coated with Au cones. The oxidation peak current ratio between BA and NB demonstrated good linearity with the logarithm of the H 2 O 2 concentration values ranging from 0.5 μM to 400 μM with a low detection limit of 0.02 μM. The developed sensor showed remarkable selectivity against potential interferences in whole blood samples, especially for ascorbic acid , uric acid , and dopamine. In combination with the unique characteristics of CFME, such as a small size and good biocompatibility , the microsensor was used for rapid analysis of one drop of whole blood sample. This sensor not only creates a new platform for the detection of H 2 O 2 in whole blood samples, but also provides a new design strategy of other ROS analysis for early diagnosis of ROS-related diseases, drug discovery processes, and pathological mechanisms.
研究领域:
生物医学工程
·
分析化学
·
病理学
✓ 已验证
DOI
PubMed
高影响因子
TALANTA
·
2024
·
IF 5.6
Songjia Luo,Qingliu Wu,Lu Wang,Hao Qu,Lei Zheng
Electrochemical aptamer-based biosensors (EABs) have been developed for multiple important biomarkers for their convenient and real-time features. However, the application of EABs in complex biological fluids has been limited by the rapid loss of sensitivity and selectivity due to inactivation and biofouling of aptamer probes and electrodes. To address this issue, we report the preparation of a simple hydrogel-protected aptamer-based biosensor (HP-EAB) for direct detection of Doxorubicin (DOX) in whole blood. The aptamer provides excellent selectivity for the electrochemical sensor, allowing the prepared sensor to accurately detect DOX in a 50-fold diluted whole blood sample. The agarose hydrogel coating on the electrode surface allows the passage of small molecules while hindering the adsorption of biomolecules from the whole blood matrix to the electrode surface. The experimental results show that the prepared HP-EAB has high stability compared with the unprotected EAB, and the HP-EAB maintains excellent detection performance after 7 days of storage. The hydrogel coating can effectively reduce the non-specific response to the whole blood matrix and prolong the life-time of the sensor. When used to detect DOX in rabbit whole blood, the HP-EAB exhibited excellent detection performance with a detection limit of 25.9 nM (S/N = 3) and a detection range of 0.1 μM–50 μM. The developed HP-EAB provides an excellent platform for the rapid and accurate determination of important analytes in complex biological fluids.
研究领域:
生物医学工程
·
分析化学
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2018
·
IF 8.173
Haifeng Zhou,Guoxia Ran,Jean-Francois Masson,Chan Wang,Yuan Zhao,Qijun Song
Biosensors based on converting the concentration of analytes in complex samples into single electrochemical signals are attractive candidates as low cost, high-throughput, portable and renewable sensor platforms. Here, we describe a simple but practical analytical device for sensing an anticancer drug in whole blood, using the detection of methotrexate (MTX) as a model system. In this biosensor, a novel carbon-based composite, tungsten phosphide embedded nitrogen-doped carbon nanotubes (WP/N-CNT), was fixed to the electrode surface that supported redox cycling. The electronic transmission channel in nitrogen doped carbon nanotubes (N-CNT) and the synergistic effect of uniform distribution tungsten phosphide (WP) ensured that the electrode materials have outstanding electrical conductivity and catalytic performance. Meanwhile, the surface electronic structure also endows its surprisingly reproducible performance. To demonstrate portable operation for MTX sensing, screen printing electrodes (SPE) was modified with WP/N-CNT. The sensor exhibited low detection limits (45 nM), wide detection range (0.01–540 μM), good selectivity and long-term stability for the determination of MTX. In addition, the technique was successfully applied for the determination of MTX in whole blood.
研究领域:
生物传感
·
材料科学
·
医学检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2022
·Vol. 27
·期 9
·2739
·
IF 4.927
Kai Wang,Luoxing Yang,Huili Huang,Ning Lv,Jiyang Liu,Youshi Liu
Rapid and highly sensitive determination of clozapine (CLZ), a psychotropic drug for the treatment of refractory schizophrenia, in patients is of great significance to reduce the risk of disease recurrence. However, direct electroanalysis of CLZ in human whole blood remains a great challenge owing to the remarkable fouling that occurs in a complex matrix. In this work, a miniaturized, integrated, disposable electrochemical sensing platform based on the integration of nanochannel arrays on the surface of screen-printed carbon electrodes (SPCE) is demonstrated. The device achieves high determination sensitivity while also offering the electrode anti-fouling and anti-interference capabilities. To enhance the electrochemical performance of SPCE, simple electrochemical polarization including anodic oxidation and cathodic reduction is applied to pretreat SPCE. The electrochemically polarized SPCE (p-SPCE) exhibits an enhanced electrochemical peak signal toward CLZ compared with bare SPCE. An electrochemically assisted self-assembly method (EASA) is utilized to conveniently electrodeposit a vertically ordered mesoporous silica nanomembrane film (VMSF) on the p-SPCE, which could further enrich CLZ through electrostatic interactions. Owing to the dual signal amplification based on the p-SPCE and VMSF nanochannels, the developed VMSF/SPCE sensor enables determination of CLZ in the range from 50 nM to 20 μM with a low limit of detection (LOD) of 28 nM (S/N = 3). Combined with the excellent anti-fouling and anti-interference abilities of VMSF, direct and sensitive determination of CLZ in human blood is also achieved.
研究领域:
分析化学
·
生物医学工程
·
药物分析
✓ 已验证
DOI
高影响因子
Nanomaterials
·
2022
·Vol. 12
·期 7
·1157
·
IF 5.719
Kai Ma,Luoxing Yang,Jun Liu,Jiyang Liu
Screen-printed carbon electrodes (SPCEs) bear great potential in the detection of biomarker in clinical samples with low sample consumption. However, modification of electrode surfaces to improve the anti-interference ability and sensitivity is highly desirable for direct electroanalysis of whole blood samples. Here, a reliable and miniaturized electrochemical sensor is demonstrated based on SPCE equipped with vertically-ordered mesoporous silica-nanochannel film (VMSF). To achieve stable binding of VMSF and improve the electrocatalytic performance, electrochemically reduced graphene oxide (ErGO) is applied as a conductive adhesion layer, that is in situ reduced from GO nanosheets during fast growth (less than 10 s) of amino groups modified VMSF (NH2-VMSF) using electrochemically assisted self-assembly (EASA). In comparison with bare SPCE, NH2-VMSF/ErGO/SPCE exhibits decreased oxidation potential of uric acid (UA) by 147 mV owing to significant electrocatalytic ability of ErGO. The dual signal amplification based on electrocatalysis of ErGO and enrichment of nanochannels leads to enhanced peak current by 3.9 times. Thus, the developed NH2-VMSF/ErGO/SPCE sensor enables sensitive detection of UA in the range from 0.5 μM to 180 μM with a low limit of detection (LOD, 129 nM, S/N = 3). Owing to good anti-fouling ability and high selectivity of the sensor, direct and rapid detection of UA in human whole blood is realized with very low sample consumption (50 μL).
研究领域:
生物医学工程
·
分析化学
·
纳米技术
✓ 已验证
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Tingting Xu,Bo Chi,Jian Gao,Meilin Chu,Wenlu Fan,Meihui Yi,Hong Xu,Chun Mao
A simple and accurate immune sensor for quantitative detection of α-Fetoprotein (AFP) was developed based on the immobilization of antigen on the surface of Hep-PGA-PPy nanoparticles modified glassy carbon electrodes (GCE). The obtained Hep-PGA-PPy nanoparticles were characterized by fourier transform infrared (FT-IR) spectra and transmission electron microscopy (TEM). And the blood compatibility of Hep-PGA-PPy nanoparticles was investigated by in vitro coagulation tests, hemolysis assay and whole blood adhesion tests . Combining the conductive property of polypyrrole (PPy) and the biocompatibility of heparin (Hep), the Hep-PGA-PPy nanoparticles could improve not only the anti-biofouling effect the electrode, but also improved the electrochemical properties of the immune sensor. Under optimal conditions, the proposed immune sensor could detect AFP in a linear range from 0.1 to 100 ng mL −1 with a detection limit of 0.099 ng mL −1 at the signal-to-noise ratio of 3, and it also possessed good reproducibility and storage stability. Furthermore, the detection of AFP in five human blood samples also showed satisfactory accuracy with low relative errors. Thus, the developed immune sensor which showed acceptable reproducibility, selectivity , stability and accuracy could be potentially used for the detection of whole blood samples directly.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2016
·
IF 4.803
Shaoming Fang,Xiaodong Dong,Shunli Liu,Donglai Penng,Linghao He,Minghua Wang,Guodong Fu,Xiaozhong Feng,Zhihong Zhang
In this paper, we report a novel label-free electrochemical aptasensor for detecting proteins in whole blood based on a three-component nanocomposite , in which ferriferrous oxide and three-dimensional graphene nanocomposite were modified with the plasma-polymerized 4-vinyl pyridine (Fe 3 O 4 @3D-rGO@PP4VP). In this novel sensing strategy, large amounts of amino groups in PP4VP facilitated the immobilization of aptamer strands via the strong electrostatic interaction between positively charged ammonium groups of the nanocomposites and negatively charged phosphate groups of aptamers. In the presence of thrombin, LYS (LYS), and platelet-derived growth factor-BB (PDGF-BB), the adsorbed aptamer strands on the developed nanocomposite surface caught the targeted proteins at the electrode interface. The aptamer preferred to be a barrier for electrons and inhibited electron transfer, leading to the decreased peak current of cyclic voltammetry measurements and the increased electron transfer resistance of electrochemical impedance spectroscopy . The determination of the thrombin, PDGF-BB, and LYS concentrations with this novel strategy showed low detection limits of 4.5, 29.4, and 14 pg·mL −1 , and the analytical ranges extend from 0.01 to 50, 0.1 to 100, and 0.1 to 200 ng·mL −1 , respectively. The resultant aptasensor exhibited high selectivity, acceptable reproducibility, and stability toward thrombin. The aptasensor could be used to detect thrombin in whole blood samples, thereby suggesting its possible application in clinical settings.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Tai-Cheng Lu,Yi-Ting Lin,Wen-Biao Xiao,Qing-Zhen Qiu,Hui-Yun Tian,Yun Lei,Ai-Lin Liu
Developing the portable CRP detection technologies that are suitable for point-of-care (POC) and primary care management is of utmost importance, and advancing the electrochemical immunosensors hold promise for POC implementation. Nevertheless, non-specific adsorption of numerous interfering proteins in complex biological media contaminates immunosensors, thereby restricting the reliability in detection efficacy. In this study, a three-dimensional flower-leaf shape amyloid bovine serum albumin/gold nanoparticles/polyaniline (AL-BSA/AuNPs/PANI) coating on the surface of the electrode was developed, which demonstrated strong anti-adsorption properties against bovine serum albumin, plasma, and cells. The immunosensor exhibited a good linear relationship to CRP response, featuring a detection limit of 0.09 μg/mL, consistent with clinical reference range. In addition, the CRP immunosensor demonstrated excellent specificity in other inflammation-related proteins and commendable anti-interference performance for CRP detection in plasma and whole blood tests. Importantly, by combining the development of a USB flash disk-type portable electrochemical workstation with a reagent-free mode, the developed CRP electrochemical immunosensor delivered ideal results in clinical samples. The anti-fouling performance, sensitivity and specificity of the immunosensor, as well as its flexible test modes in clinical samples, provide important scientific basis for developing POC detection technologies of vital biomarkers in complex biological media.
研究领域:
医学诊断
·
生物材料
·
电化学传感
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2014
·
IF 4.517
Chong Sun,Qiaorong Han,Daoying Wang,Weimin Xu,Weijuan Wang,Wenbo Zhao,Min Zhou
In this paper, we have synthesized hyperbranched polyester microspheres with carboxylic acid functional groups (HBPE-CA) and developed a label-free electrochemical aptamer biosensor using thrombin-binding aptamer (TBA) as receptor for the measurement of thrombin in whole blood. The indium tin oxide (ITO) electrode surface modified with HBPE-CA microspheres was grafted with TBA, which has excellent binding affinity and selectivity for thrombin. Binding of the thrombin at the modified ITO electrode surface greatly restrained access of electrons for a redox probe of [Fe(CN) 6 ] 3−/4− . Moreover, the aptamer biosensor could be used for detection of thrombin in whole blood, a wide detection range (10 fM–100 nM) and a detection limit on the order of 0.90 fM were demonstrated. Control experiments were also carried out by using bull serum albumin (BSA) and lysozyme in the absence of thrombin. The good stability and repeatability of this aptamer biosensor were also proved. We expect that this demonstration will lead to the development of highly sensitive label-free sensors based on aptamer with lower cost than current technology. The integration of the technologies, which include anticoagulant, sensor and nanoscience, will bring significant input to high-performance biosensors relevant to diagnostics and therapy of interest for human health.
研究领域:
生物传感技术
·
纳米科学
·
医学诊断
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）