Frontiers in Physiology
·
2020
·
IF 3.367
Li Fan,Li Ke,Wu Li-Juan,Fan Yong-Liang,Liu Tong-Xian
Oviposition is an important reproductive behavior that is triggered by mating in insects, and biogenic amines might be involved in its regulation. The effects of biogenic amines on oviposition have only been studied in a few insect species, and the findings to date have not been conclusive. In addition, there are few studies on the effects of biogenic amines on oviposition of the diamondback moth, Plutella xylostella L. Here, we tested how mating and biogenic amines regulate oviposition of P. xylostella by injecting amines and amine receptor antagonists into virgin and mated females and counting the number of eggs laid afterward. Biogenic amines of octopamine and tyramine could induce virgin adults of P. xylostella to lay eggs, while dopamine and serotonin had no such effect on oviposition. Furthermore, the octopamine antagonists mianserin, epinastine, and phentolamine inhibited oviposition by mated females. The tyramine antagonist yohimbine, dopamine antagonist SCH23390, and serotonin antagonist ketanserin did not block oviposition by mated females, and octopamine and tyramine-inducing oviposition by virgin females could be inhibited by the octopamine antagonists mianserin and epinastine instead of the tyramine antagonist yohimbine. We conclude that octopamine and its receptors are involved in mating-triggered oviposition in P. xylostella, while tyramine acts as a subsidiary. Further, the inducing effect of tyramine on oviposition is achieved via octopamine receptors instead of tyramine receptors. This experiment is helpful to further understand the role of biogenic amines in mating regulation and to provide a new strategy for controlling P. xylostella.
研究领域:
昆虫学
·
神经生物学
✓ 已验证
DOI
PubMed
高影响因子
FOOD HYDROCOLLOIDS
·
2020
·
IF 7.053
Hui Xue,Meng Xu,Mingfu Liao,Wenxiang Luo,Guowen Zhang,Yonggang Tu,Yan Zhao
Tea and illicium verum are the main raw materials of marinated egg, and play an important role in the braising process. In this study, effects of tea and illicium verum braise on the physicochemical characteristics, microstructure, and molecular structure of the heat-induced egg white protein gel were investigated. Results indicated that, with an increased in tea and illicium verum concentration, all of the moisture content, T 2 and pH of tea treated boiled egg (TTBG) and illicium verum treated boiled egg (ITBG) showed a decreased trend. The water holding capacity of TTBG showed an increased first and then remained unchanged, while that of ITBG showed a rapid increased first and then decreased continually. The free sulfhydryl group and the hardness of both TTBG and ITBG showed an increased rapidly, and especially, the hardness of ITBG reached 2161.83 g at last. The surface hydrophobicity of TTBG showed an overall increased trend, while that of ITBG showed a decreased trend with a slight red-shift. Environmental scanning electron microscopy showed that TTBG gradually formed small compact aggregates, while ITBG gradually formed hypertrophy porous aggregates. Fourier transform infrared spectrometry displayed that the intramolecular β-sheets of both TTBG and ITBG showed a decreased trend, while the intermolecular β-sheets, α-helices, β-turn and intermolecular antiparallel β-sheets showed an increased trend. In general, both tea and illicium verum could improve the gel strength of boiled egg, but in a different way, and the effect of illicium verum braise on the strength of enhancing gel was better than that of tea.
研究领域:
食品科学
·
蛋白质化学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2021
·
IF 4.556
Wei Jia,Yinghuan Yang,Shuxing Liu,Lin Shi
Irradiation technology has been shown to be a possible preservation technology used to prolong the shelf life and provide microbial decontamination of spices. In this study, the molecular mechanisms of the irradiation-induced accumulation of polyphenols in star anise were explored using metabolomics combined with ultra-high-performance liquid chromatography quadrupole-Orbitrap (UHPLC-Q-Orbitrap) high-resolution mass spectrometry. Fifty-six star anise samples from different areas processed with 2–10 kGy doses of irradiation were compared. The effect of post irradiation storage times ranging from 0 to 70 days was also evaluated. A total of 161 metabolites with variable importance on projection greater than 1 were characterized, and significant differences in flavonoid content in star anise were observed after irradiation. Absolute quantification was used for the quantification of distinctive compounds, and the limit of quantitation of the components was 1.90–6.53 μg/kg. The flavonoid content was stable on the 28th day of storage. The average content of flavonoids increased by 2–9 times due to the breaking of glycoside bonds in star anise by irradiation. C 15 H 11 O 7 + m/z 303.13045 and C 3 H 3 O 2 + m/z 71.01276 were defined as the characteristic fragments of flavonoids according to the mechanism of fragmentation. Checking the extraction ion chromatogram (EIC) characteristic fragments allows straightforward screening of untargeted flavonoids.
研究领域:
植物学
·
食品科学
·
分子生物学
✓ 已验证
DOI
高影响因子
Journal of Food Measurement and Characterization
·
2024
·
IF 2.9
Huang Zhiwu,Peng Qiang,Luo Xiaomin,Bi Yongguang,Kong Fansheng,Wang Zhong,Tan Shaofan,Zhang Junyong
Protein-stabilized star anise essential oil nano-emulsion (SAEO-NE) was successfully prepared using an ultrasound-assisted method to address the intrinsic defects and enhance the bioavailability of star anise essential oil (SAEO). In this study, the polydispersity index (PDI), particle size, and zeta potential (ZP) of SAEO-NE were used as performance indicators, and the optimal preparation conditions were determined to be an emulsifier dosage of 5%, an ultrasonic power of 270 W, and an ultrasonic time of 15 min. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) analyses revealed that the resulting nanoparticle exhibited a uniform spherical shape, with a particle size of 246.5 ± 1.35 nm and a ZP of -38.97 ± 0.45 mV. Rheological analyses further confirmed the favorable rheological characteristics of the nano-emulsion. Moreover, SAEO-NE demonstrated robust adaptability across varying salinities (100, 200, 300, and 400 mM) and pH levels (1, 3, 5, 7, 9). After storage at 4 °C for 5 weeks, the nano-emulsion maintained particle sizes below 300 nm, indicating excellent storage stability. In addition, the in vitro antioxidant evaluation showed that SAEO exhibited enhanced antioxidant activity after nano-emulsification.
研究领域:
食品科学
·
纳米技术
·
生物学
✓ 已验证
产品关联
DOI
高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Zhunjing Wang,Yongjian Xie,Maidinai Sabier,Tao Zhang,Jianyu Deng,Xuming Song,Zhihong Liao,Qingguang Li,Shengxiang Yang,Yang Cao,Xingquan Liu,Guoxin Zhou
The safe storage and protection of grain includes the exploitation of plant-based fumigants as a green control technology. This study first determined the fumigant toxicity of 12 traditional Chinese medicinal plants against adults of the rusty grain beetle, Cryptolestes ferrugineus (Stephens), one of the most ubiquitous and pestilent grain storage pests worldwide. Exposure to volatiles released from Illicium verum Hook. f. powder was found to be by far the most lethal. Illicium verum power and its volatiles eluent were then confirmed to be highly effective in killing both larvae and adults of C. ferrugineus . Star anise volatiles also showed good biological activity against Lasioderma serricorne (Fabricius) and Sitophilus zeamais Motschulsky, but not Tribolium castaneum Herbst. No significant differences were found in mortality of the adults of the rusty grain beetle when exposed at different temperatures ranging from 15−35°C. Gas chromatography-mass spectrometry (GC–MS) analysis showed that the main components of the eluent of star anise powder were trans -anethole (62.60%), estragole (23.70%), linalool (3.66%) and d -limonene (2.15%). In the fumigation test using synthetic standards of these four chemicals, only trans -anethole showed effective biological activity with exposure to 30 mL/L trans -anethole for 24 h resulting in 100% mortality of rusty grain beetle adults. Estragole did significantly enhance fumigant efficiency of trans -anethole. We further found that trans -anethole affects the beetles acetylcholinesterase activity, which was inhibited and decreased by 64.48% within 24 h. The results therefore show that trans -anethole, the major component of star anise volatiles contributes to its fumigant toxicity against C. ferrugineus by, at least partly, affecting its insect nervous system. Hence, star anise or its biologically active substances show very good potential to be used as a botanical insecticide to control C. ferrugineus .
研究领域:
昆虫学
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害虫防治
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植物化学
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2023
·
IF 8.8
Ying Xu,Jun Qi,Manman Yu,Ruishu Zhang,Hengxun Lin,Huimin Yan,Chao Li,Jingmin Jia,Yong Hu
This study aimed to determine the efficacy of star anise dietary fiber (SADF) in alleviating the oxidative damage of myofibrillar protein (MP) from the perspective of volatile components. SADF and SADF without essential oils (EOs) (NSADF) were added to oxidized MP. The addition of NSADF and SADF improved the water-holding capacity (WHC) and gel strength, with the 0.4% addition showing the highest values. Moreover, the WHC of MP from the SADF-treated group was significantly higher than that from the NSADF-treated group at the same dosage, suggesting that EOs in SADF improved the WHC through antioxidation. EOs in SADF prevented the attack of hydroxyl radicals on MP, increasing the β-sheet level and decreasing the random coil level, which was supported by the results of FT-IR, carbonyl content, and sulfhydryl content. Limonene and anisaldehyde present in EOs played an antioxidant role, and anisaldehyde could scavenge free radicals through demethoxylation.
研究领域:
食品科学
·
营养学
·
蛋白质化学
·
抗氧化研究
✓ 已验证
产品关联
DOI
PubMed
高影响因子
NATURAL PRODUCT RESEARCH
·
2022
·
IF 2.488
Jun-Ran Shao,Sen-Ju Ma,Ting Li,Xiao-Qing He,Zhen-Zhong Wang,Wei Xiao,Xin-Sheng Yao,Hai-Bo Li,Yang Yu
One new phenolic glycoside (1) and one new benzofuran derivative (2) were isolated from the leaves of Illicium dunnianum. The structures of these compounds were established by using comprehensive spectroscopic data analysis, including the 1D and 2D NMR, IR, HR-ESI-MS, electronic circular dichroism and comparison with literature data. All isolates were evaluated for the inhibition against the production of NO by LPS-stimulated RAW 264.7 macrophages.
研究领域:
药用植物学
·
药物化学
·
免疫学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Metabolites
·
2024
·Vol. 14
·期 10
·517
·
IF 3.4
Mingzhen Qin,Lu Chen,Xiaoli Hou,Wuwei Wu,Yu Liu,Yu Pan,Mengli Zhang,Zhien Tan,Danna Huang
Background: Rheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease. The fruits ofIllicium verum, which is a medicinal and edible resource, have been shown to have anti-inflammatory properties. Methods: In this study, we investigated the effects ofI. verumextracts (IVEs) on human RA fibroblasts-like synoviocytes (RA-FLS) by using a sensitive and selective ultra-high-performance liquid chromatography with high-definition mass spectrometry (UPLC-HDMS) method. We subsequently analyzed the metabolites produced after the incubation of cultured RA-FLS with IVEs. Results: IVEs inhibited the proliferation and suppressed the migration of RA-FLS, and reduced the levels of inflammatory factors including TNF-α and IL-6. Twenty differential metabolites responsible for the effects of IVEs were screened and annotated based on the UPLC-HDMS data by using a cell metabolomics approach. Discussion: Our findings suggest that treating RA-FLS with IVEs can regulate lipid and amino acid metabolism, indicating that this extract has the potential to modify the metabolic pathways that cause inflammation in RA. Conclusions: This might lead to novel therapeutic strategies for managing patients with RA.
研究领域:
药理学
·
免疫学
·
代谢组学
·
风湿病学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Arabian Journal of Chemistry
·
2023
·
IF 6
Tianming Zhao,Guang Fan,You Tai,Xinhe Shu,Fu Tian,Shuliang Zou,Qin Wu
Illicium lanceolatum is a medicinal and aromatic plant widely distributed in the south of China. The reports on chemical composition and biological activities of its essential oils (EOs) were very limited. In this study, Illicium lanceolatum EOs were extracted by hydro distillation, and analyzed by GC–MS and GC-FID. DPPH radical scavenging assay, ABTS cation radical scavenging assay and ferric reducing/antioxidant power (FRAP) assay were used for antioxidant activity evaluation. Minimum inhibitory concentrations (MICs) and minimum microbiocidal concentrations (MMCs) against 9 microorganisms were determined. The inhibitory effects on tyrosinase, α -glucosidase and cholinesterases were evaluated and cytotoxic activities were evaluated using MTT assay. The results revealed 110 identified compounds, with asaricin, eucalyptol, linalool and caryophyllene oxide as major compounds. Eucalyptol was the most abundant compound in the stem, leaf and fruit EOs while asaricin accounted for 50.52 ± 0.33 % in the root EO. Very weak radical scavenging capacities were noticed for all EOs, but the root EO showed moderate antioxidant activity (176.33 ± 4.52 mg TE/g of EO) in the FRAP assay, which could be attributed to asaricin. The root EO displayed better antimicrobial activities than other three EOs, with MIC values as 3.13 mg/mL against three bacteria including Staphylococcus aureus BNCC 186335, Bacillus cereus BNCC 103930 and Listeria monocytogenes BNCC 336877. Camphor and borneol were found to be important antimicrobial compounds. No inhibitory effect on α -glucosidase was found. The leaf EO displayed better acetylcholinesterase inhibitory activity (17.79 ± 0.32 mg GE/g of EO) while the root EO showed better tyrosinase (30.34 ± 0.40 mg KAE/g of EO) and butyrylcholinesterase (43.25 ± 1.50 mg GE/g of EO) inhibitory activities. Molecular docking between active compounds and enzymes revealed the main interactions as hydrophobic interaction, hydrogen bond and π -stacking. All EOs displayed weak cytotoxicity to HK-2 cells of normal kidney at six tested concentrations. The leaf EO showed strong anticancer activities to HepG2 cells at the concentration of 500 μg/mL. I. lanceolatum EOs showed promising prospects with possible applications in pharmaceutical and cosmetic industries.
研究领域:
药理学
·
生物化学
·
微生物学
·
植物科学
·
药物化学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）