Frontiers in Cellular and Infection Microbiology
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2025
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IF 4.8
Xiaolei Wu,Jiaxin Du,Xiao Zhou,Xianqi Peng,Chenghao Jia,Baikui Wang,Beibei Wu,Yan Li,Min Yue
Background: Monophasic Salmonella Typhimurium sequence type 34 (mSTM ST34) has emerged as a significant global health threat, but our understanding of its genomic epidemiology and potential public health implications in international and regional contexts remains limited. This study aims to fill this crucial gap by assessing the genomic epidemiology of multidrug resistance (MDR) mSTM ST34, as well as its clinical characteristics and virulence.Methods: To achieve the objectives of this study, we conducted a comprehensive genomic analysis of mSTM ST34 isolates. We obtained a global dataset comprising 13,844 strains from public databases, along with 339 strains from a regional surveillance collection in Zhejiang Province, China. This dataset aims to provide in-depth insights into antimicrobial resistance, mobile genetic elements, and pathogenicity. Additionally, we meticulously assessed the association between phenotypic profiles and clinical presentations.Results: Our findings revealed that the prevalence of mSTM ST34 has surpassed that of the previously dominant ST19. In addition, we observed an increase in the detection of the IncQ1 plasmid, which is responsible for disseminating MDR. The prevalence of mSTM ST34 carriage was exceptionally high among children (≤12 years old) and elderly individuals (≥65 years old), with 92.6% of the isolates exhibiting MDR, including resistance to frontline antimicrobials such as third-generation cephalosporins and ciprofloxacin. Additionally, the human mSTM ST34 strain demonstrates a remarkable capacity for biofilm formation, which increases its virulence in animal models and complicates therapeutic interventions.Conclusions: mSTM ST34 has surpassed the previously dominant ST19, and its ability to transmit across multi-species increases its potential for further human transmission. This study addresses critical gaps in our understanding of mSTM ST34 prevalence, highlighting the importance of whole genome sequencing in surveilling zoonotic pathogens.
研究领域:
微生物学
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流行病学
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公共卫生
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产品关联
DOI
PubMed
高影响因子
Frontiers in Public Health
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2023
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IF 5.2
Huaqi Li,Bei Liu
Background The group-type health damage caused by eco-environmental damage has been stated in the Environmental Protection Law and other laws in China. The first-ever Chinese Civil Code, which took effect in 2021, has explicitly defined eco-environmental damage relief and imposed affirmative duties on those who polluted the environment or destroyed the ecology. This study aims to describe the status quo of public health protection in eco-environmental damage relief and explore its progress and limits in protecting public health. Methods By reviewing China’s legislation on relief of eco-environmental damage and observing the implementation of these laws in judicial practice. All judicial cases of eco-environmental damage published by Chinese courts from January 2021 to May 2023 were selected and examined. From the perspective of the comparison of laws, the measurement of interests and the execution of cases, we discussed the issues of China’s legislative and judicial responses to public health. Results The relief system of eco-environmental damage in China has been formed initially, but there are still some deficiencies: In the application of law for relief of public health, there are many choices of means, resulting in the choice conflict of law application; the public health damage in the eco-environmental damage case has been ignored by courts, and it has not been a dominant consideration element; the objects of the execution of the cases are directed to the pure eco-environmental restoration, and the restoration plan does not cover public health protection measures, which does not have a preventive effect on public health. The root cause of problems is that the relief of eco-environmental damage in China follows the logic of traditional private law. Conclusion The issues mentioned above merit consideration in China’s future law revisions and judicial practice. Based on the dual nature of public and private law in environmental health, it is necessary to adjust the provisions of responsibility for eco-environmental restoration from the framework of public law, including the scope and mechanism, and then further suggestion includes the legal subject, the benefit element and the use of funds.
研究领域:
环境科学
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公共卫生学
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法学
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DOI
PubMed
高影响因子
LIFE SCIENCES
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2022
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IF 6.78
Xinyi Zhu,Henghui Li,Liuzhu Zhou,Huijun Jiang,Minghui Ji,Jin Chen
Aims Although synthetic ZnO nanoparticles (Nano-ZnO) as an alternative of ZnO compounds have been extensively used such as in livestock production, the increased consuming of Nano-ZnO has raised considerable concerns in environmental pollution and public health. Because of the low digestion of Nano-ZnO, the systematic studies on their interactions with gut microbiota remain to be clarified. Materials and methods Nano-ZnOs were prepared by co-precipitation (ZnO-cp) and high temperature thermal decomposition (ZnO-td) as well as the commercial type (ZnO-s). Transmission electron microscopy (TEM) was used to monitor the morphology of Nano-ZnO. CCK-8 assay was used for cytotoxicity evaluation. Total antioxidant capacity assay, total superoxide dismutase assay, and lipid peroxidation assay were used to evaluate oxidative states of rats. 16S rRNA was used to study the impact of Nano-ZnO on the rat gut microbiome. Key findings Both ZnO-cp and ZnO-td exhibited low cytotoxicity while ZnO-s and ZnO-td exhibited prominent antibacterial activities. After a 28-day oral feeding with 1000 mg/kg Zn at dietary dosage, ZnO-s showed slight effect on causing oxidative stress in comparison with that of ZnO-cp and ZnO-td. Results of 16S rRNA sequencing analysis indicated that ZnO-td as a promising short-term nano-supplement can increase probiotics abundances like strains belonged to the genus Lactobacillus and provide the antipathogenic effect. Significance The results of the gut microbiome alteration by synthetic Nano-ZnO not only provide solution to exposure monitoring of environmental hazard, but rationalize their large-scale manufacture as alternative additive in the food chain.
研究领域:
纳米技术
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微生物学
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毒理学
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环境科学
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公共卫生
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产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2024
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IF 8.5
Guojian Wu,Jiaqi Luo,Chenxing Du,Zhi Zheng,Yang Zhang,Pengjie Luo,Yongning Wu,Yizhong Shen
The development of facile, low-cost reliable, and precise onsite assays for the bioactive component hypoxanthine (Hx) in meat products is significant for safeguarding food safety and public health. Herein, we proposed a smartphone-assissted aggregation-induced emission (AIE) fluorogen tetraphenylethene (TPE)-incorporated amorphous Fe-doped phosphotungstates (Fe-Phos@TPE) nanozyme-based ratiometric fluorescence-colorimetric dual-mode biosensor for achieving the onsite visual detection of Hx. When the Hx existed, xanthine oxidase (XOD) catalyzed Hx into H 2 O 2 to be further catalyzed into •OH by the prominent peroxidase activity of Fe-Phos@TPE at pH = 6.5, resulting in the oxidization of nonfluorescent o -phenylenediamine (OPD, naked-eye colorless) to be yellow fluorescent emissive 2,3-diaminophenazine (DAP, naked-eye dark yellow) at 550 nm as well as the intrinsic blue fluorescence of Fe-Phos@TPE at 440 nm to be decreased via inner-filter effect (IFE) action, thereby realizing a multi-enzyme cascade catalytic reaction at near-neutral pH to overcome the traditional acidity dependence-induced time-consuming and low sensitivity troublesome.
研究领域:
分析化学
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食品科学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2024
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IF 4.9
Weili Hao,Fanghua Yin,Ke Wang,Ling Ma,Kaoqi Lian,Hongmei Shi
Currently, antibiotic residues in the environment pose a serious public health risk; therefore, new sensitive approaches for detecting the presence of antibiotics are urgently needed. Ionic-type covalent organic frameworks (TAPB-(TFIT) + Cl − -COFs) were designed and prepared in this work, and these imine-linked COFs were characterized by several techniques, proving of porous structure and rich of nitrogen. The materials were applied to the extraction of β-lactams from aqueous samples, and the important parameters of the adsorption–desorption conditions were optimized. A dispersive solid-phase extraction method coupled with ultrahigh-performance liquid chromatography-tandem mass spectrometry (TAPB-(TFIT) + Cl − -COFs-d-SPE-UPLC-MS/MS) was proposed and utilized to determine the levels of 11 antibiotics in the β-lactams in the extracted samples. Good linearity (1.0–200.0 μg·L −1 , r > 0.9999) was achieved together with limits of detection and quantification of 0.01–0.92 μg·L −1 and 0.05–2.78 μg·L −1 , respectively; recoveries ranged from 75.57 to 114.20 %, conferring relative standard deviations < 8.99 % and negligible matrix effects (84.88–109.40 %). The synthesized TAPB-(TFIT) + Cl − -COFs were demonstrated to have good reusability and high stability. Furthermore, the proposed method was clearly shown to be sensitive and separable for the reliable simultaneous detection of 11 β-lactam residues in aquatic environments.
研究领域:
环境科学
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分析化学
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药物分析
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产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
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2025
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IF 7.2
Chunjuan Yang,Li Zhang,Mengyuan Li,Xiaoming Lyu,Chaochao Ma,Shuang Jiang,Yue Zhao,Zhibin Wang,Ying Zhang,Yinghe Ji,Xuanyang Ding,Xinhui Huang,Chunli Gan,Xiaotong Wang,Yunpeng Wang,Yang Li,Yanhui Gao
Monitoring anionic pollutants in aquatic environments and biological fluids is essential for safeguarding environmental safety and human health. However, the detection of anions via surface-enhanced Raman scattering (SERS) remains challenging due to their negative charge and weak interaction with traditional substrates. Herein, we present a universal SERS platform based on calcium-induced aggregation of silver nanoparticles (Ag@BOCNPs) that enables sensitive, reproducible, and label-free detection of eight anions—including fluoride, sulfate, phosphate, and nitrate—within complex aqueous and biological matrices. The platform exhibits exceptional performance for eight ions detection, achieving ultralow limits of detection (LODs, S/N = 3) of 0.001 mg/L and limits of quantification (LOQs, S/N = 10) of 0.003 mg/L. The method demonstrates high stability and excellent anti-interference capability, allowing direct fluoride detection in human urine without pretreatment. By integrating SERS spectral data with machine learning algorithms (KNN, RF, SVM, etc.), accurate classification of anion species and quantification of fluoride exposure levels were achieved. This integrated analytical strategy not only addresses a key limitation in anion detection, but also offers a powerful tool for environmental monitoring and human exposure assessment, particularly in regions affected by water contamination and fluorosis risk.
研究领域:
环境科学
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分析化学
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纳米技术
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光谱学
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机器学习
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公共卫生
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毒理学
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产品关联
DOI
高影响因子
Nature Communications
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2023
·Vol. 14
·期 1
·1-14
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IF 16.6
Gui Yueyue,Zeng Yujing,Chen Binrui,Yang Yueping,Ma Jiehua,Li Chao
Bacterial infection is a major threat to global public health, which urgently requires useful tools to rapidly analyze pathogens in the early stages of infection. Herein, we develop a smart macrophage (Mø)-based bacteria detector, which can recognize, capture, enrich and detect different bacteria and their secreted exotoxins. We transform the fragile native Møs into robust gelated cell particles (GMøs) using photo-activated crosslinking chemistry, which retains membrane integrity and recognition capacity for different microbes. Meanwhile, these GMøs equipped with magnetic nanoparticles and DNA sensing elements can not only respond to an external magnet for facile bacteria collection, but allow the detection of multiple types of bacteria in a single assay. Additionally, we design a propidium iodide-based staining assay to rapidly detect pathogen-associated exotoxins at ultralow concentrations. Overall, these nanoengineered cell particles have broad applicability in the analysis of bacteria, and could potentially be used for the management and diagnosis of infectious diseases.
研究领域:
生物技术
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纳米技术
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医学诊断
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传染病
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产品关联
DOI
PubMed
高影响因子
Small
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2024
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IF 13
Yiming Dong,Niu Feng,Puyue Liu,Qiaoling Wei,Xuewen Peng,Feng Jiang,Yiping Chen
The improper use and overuse of antibiotics have led to significant burdens and detrimental effects on the environment, food supply, and human health. Herein, a magnetic solid-phase extraction program and an optical immunosensor based on bimetallic Ce/Zr-UiO 66 for the detection of antibiotics are developed. A magnetic Fe 3 O 4 @SiO 2 @Ce/Zr-UiO 66 metal-organic framework (MOF) is prepared to extract and enrich chloramphenicol from fish, wastewater, and urine samples, and a horseradish peroxidase (HRP)-Ce/Zr-UiO 66@bovine serum protein-chloramphenicol probe is used for the sensitive detection of chloramphenicol based on the dual-effect catalysis of Ce and HRP. In this manner, the application of Ce/Zr-UiO 66 in integrating sample pretreatment and antibiotic detection is systematically investigated and the associated mechanisms are explored. It is concluded that Ce/Zr-UiO 66 is a versatile dual-track material exhibiting high enrichment efficiency (6.37 mg g −1 ) and high sensitivity (limit of detection of 51.3 pg mL −1 ) for chloramphenicol detection and serving as a multifunctional MOF for safeguarding public health and hygiene.
研究领域:
环境科学
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分析化学
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公共健康
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产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL RESEARCH
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2024
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IF 7.7
Bingqing Yang,Jingyi Sun,Shuyao Zhu,Zhiqiang Wang,Yuan Liu
Antimicrobial resistance poses the most formidable challenge to public health, with plasmid-mediated horizontal gene transfer playing a pivotal role in its global spread. Bisphenol compounds (BPs), a group of environmental contaminants with endocrine-disrupting properties, are extensively used in various plastic products and can be transmitted to food. However, the impact of BPs on the plasmid-mediated horizontal transfer of antibiotic resistance genes (ARGs) has not yet been elucidated. Herein, we demonstrate that BPs could promote the conjugative transfer frequency of RP4-7 and clinically multidrug-resistant plasmids. Furthermore, the promoting effect of BPs on the plasmid transfer was also confirmed in a murine model. Microbial diversity analysis of transconjugants indicated an increase in α diversity in the BPAF-treated group, along with the declined richness of some beneficial bacteria and elevated richness of Faecalibaculum rodentium , which might serve as an intermediate repository for resistance plasmids. The underlying mechanisms driving the enhanced conjugative transfer upon BPAF treatment include exacerbated oxidative stress, disrupted membrane homeostasis, augmented energy metabolism, and the increased expression of conjugation-related genes. Collectively, our findings highlight the potential risk associated with the exacerbated dissemination of AMR both in vitro and in vivo caused by BPs exposure.
研究领域:
环境科学
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微生物学
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公共健康
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产品关联
DOI
PubMed
高影响因子
Engineering
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2022
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IF 12.834
Yingbo Shen,Fupin Hu,Yongqiang Wang,Dandan Yin,Lu Yang,Yiqiang Chen,Chunyan Xu,Jiyun Li,Junyao Jiang,Xueyang Wang,Yulin Fu,Dongyan Shao,Dejun Liu,Tengfei Ma,Chang Cai,Zhangqi Shen,Shaolin Wang,Juan Li,Rong Zhang,Yuebin Ke,Congming Wu,Jianzhong Shen,Timothy R. Walsh,Yang Wang
Although carbapenem use is prohibited in animals in China, carbapenem-resistant Escherichia coli (CREC), especially New Delhi metallo-β-lactamase (NDM)-producing strains, are widely prevalent in food-producing animals. At present, the impact of livestock-associated CREC strains on human populations at the national level is unknown. Here, we conduct a retrospective cross-sectional study to investigate the prevalence of CREC from clinical settings across 22 Chinese provinces or municipalities and analyze anthropogenic factors associated with their presence. We also ascertain the bla NDM and bla KPC abundance among pig and chicken farms and present a detailed genomic framework for CREC of animal and human origin. Overall, 631/29 799 (2.1%) clinical Escherichia coli ( E. coli ) isolates were identified as CREC. Multivariable analysis revealed that being male, an age below 1, an age between 13 and 18, provinces with greater chicken production, and provinces with higher pig production were associated with higher odds of CREC infection. In general, 73.8% ( n = 45/61) of pig farms and 62.2% ( n = 28/45) of chicken farms had a bla NDM abundance of 1 × 10 −5 to 1 × 10 −3 and 1 × 10 −3 to 1 × 10 −2 , respectively. Among all the Chinese NDM-positive E. coli ( n = 463) available at the National Center for Biotechnology Information (NCBI), the genomic analysis revealed that bla NDM-5 and IncX3 were the predominant carbapenemase gene-plasmid combination, while a highly homogeneous relationship between NDM-positive isolates from humans and animals was demonstrated at the plasmid and core genome levels. All the findings suggest frequent CREC transmission between humans and animals, indicating that further discussions on the use of antibiotics in animals and humans are needed, both in China and across the globe.
研究领域:
微生物学
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公共健康
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基因组学
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DOI
高影响因子
JOURNAL OF HAZARDOUS MATERIALS
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2023
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IF 13.6
Zhi Mei,Yuhao Fu,Fang Wang,Leilei Xiang,Fang Hu,Jean Damascene Harindintwali,Mingyi Wang,Marko Virta,Syed A. Hashsham,Xin Jiang,James M. Tiedje
Antibiotic resistance genes (ARGs) and human pathogenic bacteria (HPB) in leafy vegetable is a matter of concern as they can be transferred from soil, atmosphere, and foliar sprays, and poses a potential risk to public health. While traditional disinfection technologies are effective in reducing the presence of ARGs and HPB in soil. A new technology, foliar spraying with magnetic biochar/quaternary ammonium salt (MBQ), was demonstrated and applied to the leaf surface. High-throughput quantitative PCR targeting 96 valid ARGs and 16 S rRNA sequencing were used to assess its efficacy in reducing ARGs and HPB. The results showed that spraying MBQ reduced 97.0 ± 0.81% of “high-risk ARGs”, associated with seven classes of antibiotic resistance in pakchoi leaves within two weeks. Water washing could further reduce “high-risk ARGs” from pakchoi leaves by 19.8%− 24.6%. The relative abundance of HPB closely related to numerous ARGs was reduced by 15.2 ± 0.23% with MBQ application. Overall, this study identified the potential risk of ARGs from leafy vegetables and clarified the significant implications of MBQ application for human health as it offers a promising strategy for reducing ARGs and HPB in leafy vegetables.
研究领域:
环境科学
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微生物学
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农业
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公共健康
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DOI
PubMed
高影响因子
Sensors & Diagnostics
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2022
·Vol. 2
·期 1
·168-175
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IF 0
Lingfeng Xu,Yuefeng Wang,Chuan Jiang,Yanrong Huang,Genhe He,Limin Liu
Liquid safety has become one of the urgent affairs in global public health studies, and a noninvasive and effective viscosity inspection method is expected to ease the burden of continuously increasing health problems. In this study, one kind of activatable molecular sensor (DTPMP) based on a salicylaldehyde derivate has been synthesized via the Schiff base condensation reaction. This sensor is comprised of a donor–acceptor conjugated structure. We proposed the viscosity of the liquid microenvironment as a marker, and a rotatable conjugate structure was utilized as the recognition site. The molecular sensor was synthesized in a one-step facile way, and DTPMP displayed a longer emission wavelength and larger Stokes shift (142.5 nm in water, 116.8 nm in glycerol), which endows the sensor with the capacity of avoiding auto-fluorescence and achieving high signal-to-noise ratio imaging. The rotation of aromatic rings in DTPMP is restricted in a high-viscosity microenvironment, with the gradually increasing fluorescence emission signal at 532 nm. Moreover, the DTPMP sensor exhibits high adaptability, selectivity, sensitivity, and good photostability in various commercial liquids. Significantly, this sensor has successfully been applied not only in the determination of the thickening effects of food gum but also in the detection of viscosity enhancement during the liquid food spoilage process, which may be helpful in screening the metamorphic stage. We expected that this unique strategy will reinvigorate the continued perfection of the fluorescence technique toward liquid safety investigation.
研究领域:
分析化学
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生物医学工程
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公共健康
✓ 已验证
DOI
危险品化学品经营许可证（带存储）