SENSORS AND ACTUATORS B-CHEMICAL
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2025
·
IF 7.7
Pengfei Ge,Yidan Wang,Jinjie Hou,Tian Ding,Jun Zhang,Yunlei Xianyu
Detecting and eliminating bacteria efficiently is crucial to combat equipment contamination. Conventional methods are expensive, time-consuming, and incapable of simultaneous detection and killing, thereby resulting in rapid bacterial multiplication posing a major threat to public health. In this study, we present aptamer-modified gold nanoclusters (Apt-Au NCs) exhibiting excellent fluorescent and catalytic properties that combine both bacterial detection and antibacterial activity. Thiol-modified aptamer functionalized on Au NCs specifically binds to bacterial surfaces, enabling the simultaneous identification and eradication of bacteria. The specific binding of bacteria enhances the fluorescent signal of Apt-Au NCs, exhibiting a linear detection range from 10 to 10 5 CFU/mL with a LOD of 29.6 CFU/mL. The Apt-Au NCs exhibit peroxidase-like behavior, resulting in almost complete eradication of bacterial viability. Furthermore, when applied to hydrogel, Apt-Au NCs maintain their ability to detect and combat bacterial contamination, enabling fluorescence identification of bacterial presence and elimination. This work provides a promising platform for the simultaneous detection and eradication applicable to equipment-related surfaces, which holds great potential for ensuring public safety in food and medical industries.
研究领域:
纳米技术
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生物传感
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抗菌剂
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材料科学
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生物医学工程
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DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2024
·
IF 6.7
Weihong Yin,Kai Hu,Yunxing Yang,Jianjian Zhuang,Zheyu Zou,Yuanjie Suo,Liping Xia,Jiale Li,Yehong Gui,Haohua Mei,Juxin Yin,Tao Zhang,Ying Mu
Escherichia coli O157:H7 (E. coli O157:H7) is a prominent pathogenic bacterium that poses serious risks to food safety and public health. Rapid and accurate detection of live E. coli O157:H7 is of great importance in food quality monitoring and clinical diagnosis. Here, we report a propidium monoazide-assisted nonamplification digital CRISPR/Cas12a assay for sensitive and rapid detection of live E. coli O157:H7. The incorporation of propidium monoazide into the method enables the selective detection of live bacteria by eliminating 98% of interference from the dead bacterial nucleic acid. Implemented on microfluidic digital chips, this method can achieve absolute quantification of nonamplified nucleic acid. The entire detection process of live bacteria can be completed within 120 min without the need for establishing a standard curve, and the sensitivity of the method reaches 1.2 × 103 CFU/mL. The method was validated using various samples, yielding results consistent with the plate counting method (Pearson’s r = 0.9490). Consequently, this method holds significant potential for applications in fields requiring live bacterial detection.
研究领域:
生物技术
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分子生物学
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食品科学
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公共卫生
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产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Moying Yang,Yan Yao,Haotian Shen,Fei Teng,Yunuo Liu,Yuyang Ma,Bin Di,Mengxiang Su
Depression has gradually become a serious public health problem. Traditionally, data on depression are mainly derived from questionnaires or antidepressant prescriptions, and these methods may suffer from update delays and survey bias, resulting in a low actual prevalence of depression. To monitor the prevalence of depression in near real-time, this study developed an analytical method of UPLC-MS/MS by wastewater-based epidemiology to detect 10 typical antidepressants and metabolites in four provinces of China. The method has high sensitivity in that the lower limit of quantification was 2 ng/L. And the method provides recoveries and matrix effects ranging from 85.12 % to 102.98 % and 84.95 % to 112.75 %, respectively. The results showed that among the four provinces, the highest average detected concentration of antidepressants was in Jiangsu (91.82 ± 150.46 ng/L), followed by Guangxi (76.06 ± 130.44 ng/L), Anhui (61.53 ± 94.09 ng/L), and Hainan (27.87 ± 28.33 ng/L). Most wastewater treatment facilities had antidepressants such as venlafaxine, fluvoxamine, and agomelatine. The average back-calculated consumption of venlafaxine and fluvoxamine was 815.88 ± 2015.96 mg/day/1000 inh and 497.02 ± 405.21 mg/day/1000 inh, respectively. Based on this study, the estimated prevalence in the population over 15 in Jiangsu, Anhui, and Hainan was finally determined to be 4.90 %, 5.36 %, and 2.93 %, indicating regional diversity. This study offered a workable way to track the prevalence of depression using wastewater-based epidemiology, which aided public health agencies in comprehending the state of depression prevalence.
研究领域:
环境科学
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分析化学
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流行病学
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公共卫生
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药物监测
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DOI
高影响因子
ACS ES&T Water
·
2023
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IF 5.3
Xue-Ting Shao,Yue-Tong Zhao,Bing Jiang,Yan-Ying Li,Jian-Guo Lin,De-Gao Wang
Wastewater-based epidemiology has some advantages in assessing chronic diseases with high prevalence, but selecting appropriate biomarkers is difficult. The aim of this study was to screen biomarkers in wastewater to accurately assess the prevalence of hypertension (ten drugs), diabetes (eight drugs), and gout (four drugs). The concentrations of biomarkers in the influent from 37 wastewater treatment plants in 34 Chinese cities were assessed using LC/MS–MS combined with solid-phase extraction. Biomarkers with excretion rates above 5% and high stability (>80%) and detection frequency (>80%) were selected to evaluate the prevalence. Sartans, hydrochlorothiazide, sotalol, and nornifedipine have been identified as biomarkers for hypertension; metformin and glibenclamide for diabetes; and oxypurinol and febuxostat for gout. The prevalence rates of hypertension, diabetes, and gout in China assessed using these biomarkers were 26% (95CI %: 18.8–36.5), 9.58% (95CI %: 6.62–14.2), and 5.95% (95CI %: 4.22–6.74), respectively, which were consistent with government survey data. We found a co-occurrence of hypertension, diabetes, and gout prevalence, and they were associated with unhealthy lifestyle, economics, and medical level. The results suggested that wastewater-based epidemiology is an effective approach to accurately assess disease prevalence by selecting multiple biomarkers, providing valuable information for public health.
研究领域:
流行病学
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公共卫生
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环境科学
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产品关联
DOI
高影响因子
Analytical Methods
·
2022
·Vol. 14
·期 11
·1124-1133
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IF 3.532
Shan Liang,Li Ji,Yingying Zhong,Tiantian Wang,Huiyi Yang,Qing-Lan Li,Xiangguang Li,Suqing Zhao
Infections from invasive Listeria monocytogenes (L. monocytogenes) frequently occur in food and can cause high morbidity and death. Thus, the sensitive, specific, and rapid detection of L. monocytogenes is critical for ensuring food safety and public health. Herein, a fluorescence immunoassay for trace L. monocytogenes detection was designed based on guinea pig antibody-functionalized magnetic nanoparticles (Fe3O4 NPs/pAb1) and rabbit antibody-anchored CdZnTe quantum dots (CdZnTe QDs/pAb2). Because of the antibody-directed magnetic separation and long-wave fluorescent emission for CdZnTe QD indication, the constructed immunoassay strategy presented excellent anti-interference performance toward a biological matrix. The immunosensor exhibited a wide detection range of 1 to 109 CFU mL−1 for L. monocytogenes and a low limit of detection (LOD) of 1 CFU mL−1, achieving an exceptionally sensitive detection of trace L. monocytogenes. Meanwhile, the immunosensor showed good specificity and had a short time-consumption of 60 min to realize the accurate determination of trace Listeria monocytogenes in spiked tap water and pasteurized milk samples.
研究领域:
食品科学
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分析化学
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微生物学
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生物技术
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产品关联
DOI
PubMed
高影响因子
FOOD AND AGRICULTURAL IMMUNOLOGY
·
2018
·
IF 2.568
Wenliang Ge,Steven Suryoprabowo,Xiaoling Wu,Qiankun Zheng,Hua Kuang
The misuse of β-agonists constitutes a potential risk to public health and β-agonists has been forbidden in many countries. In this study, we describe an immunochromatographic test strip method for the rapid, sensitive, and inexpensive detection of mabuterol (MAB) and mapenterol (MAP) by cross-reactivity in pig urine. For the immunochromatographic test, the coating antigen which was obtained by conjugating the salbutamol to bovine serum albumin and a goat anti-mouse IgG antibody were drawn onto a nitrocellulose membrane as the control line and test line. The test was developed with a sensitive monoclonal antibody specific for MAB, which was generated by immunizing BALB/c mice with a well-characterized MAB- keyhole limpet haemocyanin conjugate, produced in our laboratory. Under optimized conditions, the cut-off limits of the semi-quantitative test strip for MBA and MPA were as low as 10 ng/mL in both 0.01 M PBS (pH 7.4) and pig urine. All the results were obtained within 5 min. Our data indicate that the immunochromatographic assay allows provide sensitive, rapid, and specific on-site screening for MAB and MAP.
研究领域:
兽医学
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分析化学
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公共卫生
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产品关联
DOI
高影响因子
ACS Applied Bio Materials
·
2024
·
IF 4.6
Kai Liu,Qi You,Rohil Jawed,Dong Han,Yufei Miao,Xiang Gu,Junming Dong,Christopher J. Butch,Yiqing Wang
Protective masks are critical to impeding microorganism transmission but can propagate infection via pathogen buildup and face touching. To reduce this liability, we integrated electrospun photocatalytic graphitic carbon nitride (g-C3N4) nanoflakes into standard surgical masks to confer a self-sanitization capacity. By optimizing the purine/melamine precursor ratio during synthesis, we reduced the g-C3N4 band gap from 2.92 to 2.05 eV, eliciting a 4× increase in sterilizing hydrogen peroxide production under visible light. This narrower band gap enables robust photocatalytic generation of reactive oxygen species from environmental and breath humidity to swiftly eliminate accumulated microbes. Under ambient sunlight, the g-C3N4 nanocomposite mask layer achieved a 97% reduction in the bacterial viability during typical use. Because the optimized band gap also allows photocatalytic activity under shadowless lamp illumination, the self-cleaning functionality could mitigate infection risk from residual pathogens in routine hospital settings. Both g-C3N4 and polycaprolactone demonstrate favorable biocompatibility and biodegradability, making this approach preferable over current commercially available metal-based options. Given the abundance and low cost of these components, this scalable approach could expand global access to reusable self-sanitizing protective masks, serving as a sustainable public health preparedness measure against future pandemics, especially in resource-limited settings.
研究领域:
材料科学
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纳米技术
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生物医学工程
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公共卫生
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产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Jun Yao,Sijia Zheng,Xinlei Wu,Yingjie Guo,Yan Li,Yifang Mi,Zhihai Cao,Qinmin Cui
The development of textiles with durable antifouling and antibacterial properties is essential to inhibit many healthcare-associated infections and fulfill the increasing demand for public health. However, it is a challengeable task to build high-performance antibacterial surfaces on fabrics without compromise of their intrinsic serviceability in an eco-friendly way. Herein, we reported the fabrication of durable antibacterial cotton fabrics through a one-bath pad−dry−cure technology by using eco-friendly quaternized short perfluoroalkyl polymer/Ag nanocomposite particles (qf-polymer/Ag NCPs) as the finishing agents. Each fiber of the finished cotton fabrics was covered by a low surface energy thin qf-polymer/Ag composite film (CF), allowing the finished fabrics to keep the intrinsic serviceability of fabrics for a comfortable wearing feeling. The qf-polymer/Ag CF-coated cotton fabrics demonstrated a superior long-term broad-spectrum antibacterial ability against normal and multiple drug resistant bacteria. An antibacterial mechanism of the qf-polymer/Ag CF-coated cotton fabrics based on the synergism of qf-polymers, Ag NPs, and textile structure was proposed. This work provides an inspiring insight into the design and fabrication of advanced durable antibacterial surfaces on fabrics.
研究领域:
纺织科学
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材料科学
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纳米技术
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生物医学工程
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环境科学
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产品关联
DOI
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Qian Wang,Jingfeng Gao,Hao Chen,Ying Liu,Xiaoyu Fu,Jiawen An,Hanyi Wang,Yuxuan Wang
Antibiotic resistance has turned into a focus of universal public health concern. Wastewater treatment plants are considered as reservoirs of antibiotic resistant bacteria (ARB) and antibiotic resistant genes (ARGs), which can be transmitted to the environment. In this research, an effective method for inactivating Escherichia coli DH5α ( E. coli DH5α) carrying resistance genes was developed utilizing dual oxidant system of peroxymonosulfate (PMS) and sodium percarbonate (SPC) in the presence of ferrous ions (Fe(II)). The results indicated that E. coli DH5α could be inactivated 6.36 log under the conditions of 0.50 mM PMS, 0.50 mM SPC and 0.50 mM Fe(II) after 30 min. Besides, the removal of different types of ARB and pollutants could also be achieved by Fe(II)/PMS/SPC system. The quenching experiments and EPR analysis demonstrated that reactive species (•OH, SO 4 •− , O 2 •− , 1 O 2 and CO 3 •− ) involved in the inactivation of E. coli DH5α. Bacterial damage mechanisms were systematically studied in terms of cell structure and morphology, enzyme activity, malondialdehyde and intracellular reactive oxygen species levels. The inactivation of E. coli DH5α in complex water matrix (including coexisting of anions and natural organic matter) and real wastewater was inhibited. The abundance of intracellular ARGs decreased by 1.15 log, whereas extracellular ARGs increased by 0.32 log. This research supplied a prospective approach for inhibiting the dissemination of antibiotic resistance.
研究领域:
环境科学
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微生物学
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水处理
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公共卫生
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产品关联
DOI
高影响因子
TALANTA
·
2025
·
IF 6.1
Huifang Wu,Qiqi Wang,Lingfeng Zhu,Haoliang Zhang,Lizhong Liu
Anthrax, a fatal infectious disease caused by Bacillus anthracis ( B. anthracis ), poses a significant threat to public health. Herein, a novel type of carbon dots rich in π → π∗ transitions ( R -CDs) was developed to fabricate an R -CDs/Eu 3+ hybrid for the rapid on-site detection of the B. anthracis biomarker dipicolinic acid (DPA). The R -CDs/Eu 3+ hybrid exhibits two distinct fluorescence responses to DPA: (1) quenching of the R -CDs fluorescence due to the internal filter effect, and (2) enhancement of Eu 3+ fluorescence resulting from the antenna effect and the protective role of R -CDs, enabling ratiometric detection of DPA over a range of 0.1–100 μM with excellent selectivity. Furthermore, a portable, cost-effective device based on the hybrid was developed, enabling in-filed detection of DPA in lake water and real-time monitoring of DPA release from bacterial spores, which holds great promise for monitoring pathogenic biomarkers, with significant implications water safety and human health.
研究领域:
生物化学
·
分析化学
·
公共卫生
✓ 已验证
产品关联
DOI
PubMed
高影响因子
LANGMUIR
·
2025
·
IF 3.9
Huali Zuo,Yongjun Ye,Shiyu Mou,Xuanli Yao
Radon (Rn) is a radioactive inert gas, a major source of natural radiation exposure to humans, and a leading cause of lung cancer in nonsmokers. In this study, a method combining micropores and metal sites was employed. Bamboo activated carbon, modified with alkali treatment to create different pore structures, was selected as the carrier, onto which silver and nickel metals were loaded. The goal was to optimize the loading amount and improve the Rn adsorption performance of the bamboo activated carbon, developing a highly efficient Rn adsorbent. Nickel nitrate impregnation treatment created micropores in the carrier, while silver nitrate obstructed the micropore structure. The optimal impregnation concentration varied depending on the pore structure of the carrier. After nickel was loaded onto the unmodified BC, the Rn adsorption coefficient increased. For the alkali-modified BCK0.75, which has the highest proportion of key pore volume for Rn adsorption, silver loading led to a decrease in the Rn adsorption coefficient. However, for the alkali-modified BCK1.5, which has pore sizes larger than the key size for Rn adsorption, the Rn adsorption coefficients increased after loading with nickel and silver, reaching 11.36 ± 0.21 and 9.25 ± 0.12 L/g, respectively representing improvements of 75.58 and 42.97%. Additionally, the proportion of micropore volume at 0.77 nm in Ni0.3@BCK1.5 was higher than that in BCK0.75. After five cycles of heating regeneration under nitrogen, Ni0.3@BCK1.5 and Ag0.6@BCK1.5 maintained 93.88 and 92.48% of their original Rn adsorption coefficients, respectively. Ni0.3@BCK1.5 not only contains metal sites but also retains the key pore size for Rn adsorption, which has good application value in Rn removal.
研究领域:
环境科学
·
材料工程
·
核技术
·
化学工程
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公共卫生
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产品关联
DOI
PubMed
高影响因子
WATER RESEARCH
·
2022
·
IF 13.4
Di Zhang,Li Chen,Shengkun Dong,Jiayi Luo,Zuxin Xu,Wenhai Chu
In China, urea degradants are often used in combination with chlorine in swimming pool water to remove urea. Here we report the first study about the impacts of urea degradants on urea degradation, disinfection byproduct (DBP) formation and estimated DBP-associated cytotoxicity and genotoxicity. Firstly, four groups of typical DBPs from six real indoor swimming pools were analyzed. The concentration of DBPs in swimming pool waters was about 1-2 orders of magnitude higher than that source tap water. Notably, a rapid increase in DBPs was observed after urea degradant treatment. Simulated processes of urea removal using three commonly used urea degradants were therefore conducted in laboratory. Neither urea degradant nor chlorine alone removed urea effectively within 48 h. When applied in combination, urea degradant with sufficient chlorine rapidly removed urea by 100% within 3 h, and anti-chlorourea oligomer in urea degradants was the main contributor. Meanwhile, a remarkable increase in DBPs, especially brominated DBPs, was observed due to bromide introduction by urea degradants. For this reason, bromine incorporation factor (BIF) of DBPs dramatically increased. For instance, the BIF of dihaloacetic acids increased by 2665%-4025% after applying three urea degradants. As the highly toxic brominated DBPs were generated, attention should be paid into the potential DBP-related health risks from the use of urea degradants together with chlorine.
研究领域:
环境科学
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公共卫生
·
毒理学
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DOI
PubMed
高影响因子
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