JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY
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2018
·Vol. 96
·期 1
·15-23
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IF 1.601
Saisai Li,Yan Li,Nengbing Long,Ruifeng Zhang
Phospholipase D (PLD) was effectively immobilized on a nanocomposite support composed of ZnO nanowires and SiO 2 through in-situ co-cross-linking with poly-L-lysine (PLL). An anionic and long-chained bisepoxide cross-linker was electrostatically adsorbed on the surface of ZnO nanowires prior to co-cross-linking. Under the optimal pH, 6.5, the loading amount of PLD reached an average of 114 mg per g support for PLL concentrations in the range of 0–0.6 mg mL −1 . Co-cross-linking of PLD with PLL enhanced the activity of PLD remarkably. The highest specific activity of co-cross-linked PLD reached 25.8 U per mg protein , which was 1.62 times as high as that of the cross-linked PLD (15.9 U per mg protein ). (One unit of PLD activity [U] refers to the amount of PLD, which liberates 1 μmol of p-nitrophenol per min via hydrolysis of phosphatidyl-p-nitrophenol at pH 8.0 and 30 °C). Under the optimized conditions, the yield of phosphatidylglycerol (PtdGro) reached 98.6% within 80 min at 45 °C and pH 6.5. The co-cross-linked PLD not only exhibited good resistance to pH and heat inactivation but also improved storage stability and reusability. The yield of PtdGro remained ≥85.4% after 13 repeat uses.
研究领域:
生物化学
·
酶工程
·
材料科学
✓ 已验证
DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2018
·
IF 3.927
Xiao He,Ayang Zhou,Chang Shi,Jinli Zhang,Wei Li
A co-crosslinked poly(ether imide) (PEI) material with excellent organic solvent resistance was synthesized using mixed amines of aliphatic ethylenediamine (EDA) and aromatic p-xylylenediamine (XDA), on which the organic solvent nanofiltration (NF) membrane was prepared via the interfacial polymerization between the monomer 1,2,4,5-benzene tetracarbonyl chloride (BTC) and the amine terminals on the co-crosslinked PEI. Structural characterizations indicate that the EDA-XDA co-crosslinking is an effective pathway to modulate the species and the amount of amine terminals on the surfaces, which can in turn affect the toplayer thickness and the surface roughness of the synthesized NF membrane. The results illustrate that the NF membrane prepared on the EDA-XDA (1:1) co-crosslinked PEI (c-P/1E1X) possesses superior separation performance and stability for N,N-dimethylformamide (DMF) solution. Further, the superior solvent resistant NF membrane gly-NF-c-P/1E1X-XDA was synthesized through an additional quick patching-like IP using XDA, with the aid of ethylene glycol filling inside the intermediate pores. The membrane gly-NF-c-P/1E1X-XDA shows the DMF permeance of 6.1 L m −2 h −1 MPa −1 and the Rose Bengal (RB) rejection about 89%, and for a 5 g/L ceftazidime antibiotic DMF solution, the permeance is 4.6 L m −2 h −1 MPa −1 and the ceftazidime rejection is 81%, which is promising to be applied for the recovery of heat sensitivity ceftazidime in DMF solution.
研究领域:
材料科学
·
化学工程
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
BIO-MEDICAL MATERIALS AND ENGINEERING
·
2015
·
IF 1.091
Wang Xiaotong,Ma Bing,Chang Jiang
BACKGROUND: Vascular extracellular matrices (vECMs) have shown potential for small-diameter blood vessel tissue engineering applications. However, problems such as chemical instability and easy calcification are still remained. Chemical crosslinking using crosslinkers such as glutaraldehyde (GA) can improve mechanical properties and proteolysis resistance of vECMs, but leads to calcification and cytotoxicity. Procyanidins (PC) can crosslink ECMs with anti-calcification property and cytocompatibility, but the mechanical properties and chemical stability are unsatisfactory. OBJECTIVE: A novel co-crosslinking technique using PC and GA was developed, which combines the advantages of both PC and GA for enhancing mechanical properties and stability of vECMs with reduced calcification and cytotoxicity. METHODS: Fresh carotid were decellularized and then crosslinked by PC and subsequent GA for 6 h respectively. The mechanical properties, dynamic release of PC, enzymatic degradation, calcification and cytotoxicity of crosslinked samples were evaluated. RESULTS: The co-crosslinked vECMs showed enhanced tensile strength, chemical and biological stability, comparable anti-calcification property as compared to pure PC-crosslinked samples. Cytotoxicity assay showed that the co-crosslinked vECMs were cytocompatible for supporting the adhesion and proliferation of HUVECs. CONCLUSIONS: Co-crosslinking with PC and GA might be a useful method for preparation of vECM scaffolds with potential applications in small-diameter blood vessel tissue engineering.
研究领域:
组织工程
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生物材料科学
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生物医学工程
✓ 已验证
DOI
PubMed
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Xing Su,Hao Wang,Zhuoling Tian,Xiaocen Duan,Zhihua Chai,Yuting Feng,Yanxia Wang,Yi Fan,Jianyong Huang
Antifreezing gels are promising in diverse engineering applications such as structural soft matters, sensors, and wearable devices. However, the capability of fast self-healing and reversible adhesiveness still remain a huge challenge for gels at extreme temperatures. Here, we proposed a solvent-involved cross-linking system composed of polyacrylic acid, polyvinyl alcohol, borax, ethylene glycol, and water, capable of antifreezing below −90 °C. It was not only antifreezing, anticrystalline, and abundant in dynamic bonds but also highly transparent, stretchable (over 800%), and conductive over the scope of temperature from −60 to 60 °C. Moreover, this gel could self-heal within 1 min and repeatedly adhere to multiple substrates including glass, metal, and rubber with an adhesive strength greater than 18 kPa. These key functions of the gel could be mostly preserved after 5 days of storage at 70% relative humidity. It is anticipated that our research opens a new scope for high-performance extreme environment-tolerant adhesives or wearable devices.
研究领域:
材料科学
·
化学工程
·
生物医学工程
✓ 已验证
DOI
高影响因子
危险品化学品经营许可证（带存储）