JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Ying Peng,Zhi-Gang Wang,Bao-Ping Qi,Cui Liu,Bo Tang,Zhi-Ling Zhang,Shu-Lin Liu,Dai-Wen Pang
Carbon nanodots (C-dots) with good biocompatibility have been extensively utilized as co-reactants for electrochemiluminescence (ECL) of the tris(2,2′-bipyridine)ruthenium(II) (Ru(bpy) 3 2+ ) system. However, the ECL intensity of this system is still relatively low and the mechanism of C-dots as co-reactants remains unclear, which greatly limits its further application in bio-analysis. In this work, we revealed that the carboxyl groups on C-dots are co-reactant sites for Ru(bpy) 3 2+ ECL by systematically investigating the contribution of carboxyl, hydroxyl and carbonyl groups on the surface of C-dots to the ECL intensity. Further treatment with hydrogen peroxide to increase the carboxyl-group content on C-dots resulted in a 10-fold increase in ECL intensity over the original Ru(bpy) 3 2+ /C-dots system. This work provides new insights for the rational design of ECL systems with C-dots as co-reactants and offers new chances for further applications of C-dots in the field of ECL.
研究领域:
分析化学
·
生物医学工程
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DOI
高影响因子
ANALYST
·
2011
·Vol. 137
·期 3
·588-594
·
IF 3.913
Xiaoquan Lu,Dong liu,Jie Du,Haifeng Wang,Zhonghua Xue,Xiuhui Liu,Xibin Zhou
The novel phenomenon of cathodic electrochemiluminescence on a gold electrode in tris(bipyridine) ruthenium(II) (Ru(bpy)32+) solution is described for the first time. A cathodic electrochemiluminescence (ECL) was found to mainly occur at 0.4–0.8 V with continuous potential scanning from 0.2–1.4 V and the ECL peak was observed around 0.68 V, which was quite different from generally reported Ru(bpy)32+ ECL. Our group speculated that Ru(bpy)32+ possibly reacts with the gold electrode in the acidic phosphate buffer solution (PBS) to generate luminescence. The possible ECL mechanism was discussed according to the presented results. Moreover, it is revealed that the Au as co-reactant in the Ru-system contributed dominantly to the whole ECL . Therefore, the reaction between Ru(bpy)32+ and the newly formed Au implied that the inert metal Au could become a promising material for ECL investigations.
研究领域:
分析化学
·
电化学
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DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2017
·
IF 4.798
Yinggui Zhu,Min Zhao,Xiaojuan Hu,Xiaofang Wang,Ling Wang
In this paper, we report a novel rare earth metal complex with the weak ligand of aromatic sulphonic acid (pyridine-3-sulfonic acid, 3-pSO 3 H), and characterized by FT-IR, UV–vis, energy-dispersive X-ray spectroscopy (EDX), electrochemiluminescence spectra, etc. Then an excellent electrochemiluminescence (ECL) signal was observed with K 2 S 2 O 8 as the coreactant in NaAc-HAc buffer solution. For another thing, the electrochemical properties of the compound have been thoroughly investigated in acetonitrile solution, the possible ECL reaction mechanism was proposed as well. Furthermore, a simple and straightforward ECL platform was reported for sensitive and selective detection of Cd 2+ due to the effective quenching after addition of Cd 2+ . Other heavy/transition metal ions do not interfere with the sensing. The limit of detection is determined as 0.13 nM, the results suggested that as-prepared complex could be a promising material for developing ECL senors to detect the Cd 2+ rapidly indwell in environmental and practical samples.
研究领域:
分析化学
·
环境科学
·
材料科学
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DOI
高影响因子
MICROCHIMICA ACTA
·
2020
·Vol. 187
·期 7
·1-9
·
IF 6.232
Wang Caixia,Li Mengsi,Wang Peijin,Liu Defang
An electrochemiluminescence (ECL) analytical platform is constructed based on boron nitride quantum dots (BNQDs) as a novel coreactant of luminol for quantitative assay of concanavalin A (Con A). Different from previous research that mainly focuses on its superior optical properties, BNQDs are used for the first time as a coreactant for boosting ECL intensity of luminol, which has a 10-fold enhancement compared with individual poly(luminol/aniline) nanorods loaded on reduced graphene oxide (PLA-rGO) using GCE. On the basis that BNQDs contain an abundance of active amino, a possible mechanism of amino oxidation facilitating ECL emission is proposed. Firstly, luminol as light spices are oxidized to luminol •− and BNQDs generate an abundance of BNQDs-NH +• via electrochemical oxidization, producing reductive intermediates BNQDs-N • in alkaline conditions. Finally, BNQDs-N • react with luminol •− to obtain the excited species AP 2−* , returning to ground state and emitting light. Due to the hindrance effect of Con A, the ECL intensity decreases gradually as various concentrations of Con A are modifying the electrode surface. Therefore, a sensitive ECL biosensor for detecting Con A is constructed exhibiting a wide linear range of 1.0 pg·mL −1 to 1.0 μg·mL −1 and a low detection limit of 0.15 pg·mL −1 .
Schematic representation of an electrochemiluminescence (ECL) biosensor based on boron nitride quantum dots (BNQDs) as an efficient coreactant of reduced graphene oxide functionalized poly(luminol/aniline) (PLA-rGO) for quantitative assay of concanavalin A (Con A).
研究领域:
分析化学
·
纳米技术
·
生物传感器
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DOI
PubMed
高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2022
·Vol. 414
·期 29
·8309-8315
·
IF 4.478
Zhang Huixin,Du Lin,Wei Zhihao,Wang Xuemei,Sojic Neso,Zhou Xin,Wang Zonghua
Electrochemiluminescence (ECL) of luminol is a well-established methodology in analytical chemistry and bioimaging. Developing novel strategies to enhance the ECL signal of this model emitter is a challenging but rewarding task. In this work, we introduced the high-intensity focused ultrasound (HIFU), as a pretreatment means and a non-invasive way to trigger and boost the ECL signal with a 40-fold significant enhancement in the luminol-O 2 system without the addition of exogenous co-reactants. The superoxide anion (O 2 −• ) generated in situ by HIFU was the key initiator for boosting the ECL emission as demonstrated in this study for the first time. This promising co-reactant-free strategy could find potential applications for ultrasensitive ECL detection in the analysis of complex biological entities. Graphical abstract
研究领域:
分析化学
·
生物成像
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超声技术
·
生物分析
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2021
·
IF 6.986
Xinyang Zhu,Huanhuan Xing,Yuan Xue,Jing Li,Erkang Wang,Shaojun Dong
A chemical fixation strategy originating from single-atom-anchoring with metal–organic frameworks as a carrying matrix was proposed for solid-state electrochemiluminescence (ECL). Herein, UiO-67(N) with the exposure of 2,2′-bipyridine (bpy) ligands could coordinate with Ru2+ to form a local structure of [Ru(bpy)3]2+ (Ru-UiO). The influence of the steric effect induced with different Ru sources was discussed. The as-obtained Ru-UiO exhibits high ECL intensity and outstanding stability in the presence of a coreactant at low concentrations. The proposed synthesis strategy may hold great potential for the synthesis of solid-state ECL materials and their further utilization in ECL analysis.
研究领域:
材料科学
·
分析化学
·
电化学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2019
·
IF 6.35
Muhammad Saqib,Shahida Bashir,Haijuan Li,Chuanping Li,Shanshan Wang,Yongdong Jin
Developing rapid, specific, and low-cost electrogenerated chemiluminescence (ECL) systems remains a compelling goal for many applications including environmental analysis, clinical diagnostics, and food safety. In this study, N-hydroxysuccinimide (NHS) esters, especially N-hydroxysulfosuccinimide (NHSS), was exploited for the first time as an efficient coreactant for anodic Ru(bpy)32+ ECL in neutral medium. Among the three tested NHS esters (NHS, NHSS, NHPI), the NHSS exhibited much higher ECL intensity and stability than the sulfonate-free esters, suggesting the essential role of both the N-hydroxy (NOH) and sulfonate (SO3H) moieties in the developed Ru(bpy)32+ ECL system. The ECL generation process is rather straightforward, not requiring time-consuming fabrication/modification steps of electrodes. The anodic Ru(bpy)32+–NHSS system achieved ECL detections with high selectivity and sensitivity toward l-proline (LOD = 50 nM) and Hg2+ (LOD = 10 nM), with a linear range of 0.5–200 μM and 0.1–25 μM, respectively. The method showed good recoveries (95.2–104.8%) for l-proline and Hg2+ detection in real samples. This study is a successful step toward the development of new coreactants and may open new avenues for the applications of N-hydroxy compounds in ECL sensing.
研究领域:
分析化学
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环境科学
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临床诊断
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食品安全
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产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Ruru Chai,Jing Zeng,Alai Hailaxi,Yueling Song,Zhengchong Shen,Guixin Li
This study presents a highly sensitive cysteine (Cys) sensing platform based on a solvent-responsive aggregation-induced electrochemiluminescence (AIECL) probe. By modulating the DMF/PBS solvent ratio, the aggregation state of tetraphenylethylene derivative (TPE-1-CHO) was optimized, resulting in a significant enhancement of its cathodic electrochemiluminescence (ECL) performance. Experimental results revealed that at an aqueous fraction ( fw ) of 50%, the aggregation degree of TPE-1-CHO and its ECL efficiency reached an optimal balance, yielding an ECL intensity substantially higher than that in pure DMF ( fw = 0%). Based on this phenomenon, an AIECL sensing platform was constructed using K 2 S 2 O 8 as a co-reactant, and the proposed platform can effectively discriminate structurally similar biomolecules based on their reducing functional group. Given its strong ECL suppression, Cys was chosen as the target analyte to assess the analytical characteristics of the presented AIECL sensing strategy. The developed sensor exhibits exceptional sensitivity, outstanding stability, and favorable selectivity. The method was effectively utilized for the quantitative detection of Cys in human serum samples. This study offers an innovative approach for the detection of reducing bioactive small molecules.
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DOI
PubMed
高影响因子
ACS Sensors
·
2022
·
IF 9.618
Li-Ping Zong,Xiaozhong Chen,Dunru Zhu,Xi-Jie Li,Feng Li,Serge Cosnier,Xue-Ji Zhang,Robert S. Marks,Dan Shan
A novel electrochemiluminescence (ECL) amplification strategy was established aiming to overcome the inherent shortcomings of the current oxygen (O2) coreactant ECL systems. Macrocyclic Schiff base Fe complexes were rationally designed as a novel integrated ECL emitter by iminium linkage between N-(4-aminobutyl)-N-ethylisoluminol (ABEI) and 1,10-phenanthroline-2,9-dicarbaldehyde (PDL) and postmetalation of the macrocyclic Schiff base. Covalently combining luminophore ABEI with a catalytic center endowed the novel ECL emitter with both remarkable redox electrocatalytic properties and significantly enhanced ECL efficiency. The high content of ferrous iron and the dominantly active low-spin Fe state greatly contributed to the inherent catalytic activity for O2 activation. The rational modification of luminophore optimized the spatial distribution and simultaneously shortened the species transport distance of coreactant radicals generated in situ from dissolved O2, resulting in significantly self-enhanced ECL efficiency. Neomycin, which posed a growing threat to aquatic biodiversity and environmental safety, as the model antibiotic was successfully detected with a detection limit of 0.21 pM (S/N = 3), clarifying a promising application prospect of this new luminophore-embedded ECL amplification strategy in biological analysis and environmental monitoring.
研究领域:
分析化学
·
环境科学
·
生物分析
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产品关联
DOI
PubMed
高影响因子
ADVANCED FUNCTIONAL MATERIALS
·
2025
·
IF 19
Qiaoting Yang,Junhao Deng,Dunru Zhu,Fei Yu,Yi-Xuan Li,Jérome Chauvin,Xue-Ji Zhang,Serge Cosnier,Dan Shan
Electrochemiluminescence (ECL) platforms are often constrained by aggregation-caused quenching (ACQ), inefficient charge transfer, and instability under high driving potentials, thereby limiting their sensitivity and reproducibility. Herein, an energy-level rule-guided strategy is reported to construct a distinctive lanthanide-organic framework (LnOF), [Tb 2 ( µ 2 -OH) 2 (TBAPy)], through the controlled self-assembly of Tb 3+ and 4,4′,4″,4‴-(pyrene-1,3,6,8-tetrayl)tetrabenzoic acid (H 4 TBAPy). This LnOF features the rod-shaped [Tb( µ 2 -OH) 2 (-CO 2 ) 2 ] chains, a large π -stacking distance (6.685 Å) between pyrene rings, and a 3D sea topology, effectively suppressing ACQ and antenna-type energy transfer from the TBAPy triplet state to Tb 3+ , thereby preserving pyrene-based fluorescence while inhibiting the luminescence of Tb 3+ . Importantly, the low-coordinated [TbO 6 ] octahedron exposes multiple open metal sites to coordinate with coreactant S 2 O 8 2− and facilitate its decomposition to SO 4 •− radicals during electrochemical reactions. This process markedly reduces interfacial resistance and enables robust low-bias ECL amplification. Furthermore, a MnO 2 interfacial gate enables ratiometric ECL OFF/ON switching upon glutathione reduction, delivering internally referenced signal outputs with an ultralow limit of detection of 6.8 × 10 −4 n m across a wide range (0.01–500 n m ). This kind of functional material design establishes a generalizable blueprint for LnOF-based ECL systems, advancing programmable and low-bias luminescent sensing with exceptional sensitivity and stability.
研究领域:
材料科学
·
分析化学
·
电化学
·
发光材料
·
传感器设计
·
纳米技术
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产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2019
·
IF 9.518
Caixia Wang,Liming Chen,Peijin Wang,Mengsi Li,Defang Liu
In this work, an ultrasensitive electrochemiluminescence (ECL) biosensor was constructed using poly- L -lysine (PLL) as a novel co-reactant of luminol and poly(luminol/aniline) nanorods loaded reduced graphene oxide ( [email protected] ) as nanoprobe, which enable highly sensitivity detection of glutathione (GSH). To the best of our knowledge, it is the first time that PLL was used for the co-reactant of luminol. Notably, about a 5-fold enhancement was obtained compared with the individual [email protected] using GCE . Due to the remarkable quenching effect between the excited state of PLL and the reduced form of GSH in the ECL system of luminol/PLL, the ECL sensing platform exhibited wide linear ranges of 1.0 × 10 −9 –1.0 × 10 −4 M and 1.0 × 10 −4 –1.0 × 10 −2 M and a low detection limit of 7.7 × 10 −10 M. Simultaneously, the biosensor was also successfully applied to detect GSH in human serum sample with high recoveries. Hence, this work would open a new platform for the wide application of PLL in immunoassay and various sensors.
研究领域:
分析化学
·
生物传感器
·
电化学
·
纳米技术
·
生物分析化学
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产品关联
DOI
PubMed
高影响因子
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