CHEMISTRY-A EUROPEAN JOURNAL
·
2024
·
IF 3.9
Jialian Ding,Bin Su
Graphical One plus one is greater than two: A remarkable electrochemiluminescence (ECL) enhancement of Ru(bpy) 3 2+ /TPrA is observed by introducing another coreactant, TEOA, with a 10.8-fold increase of ECL intensity and 1.7-fold extension of ECL layer. The enhancement was interpreted by a chemical oxidation route, in which TEOA + ⋅ acts as an enhancer to produce more TPrA + ⋅ and meanwhile allow the remote formation of TPrA + ⋅. This dual-coreactants strategy works not only in solution with freely diffusive Ru(bpy) 3 2+ but also on Ru(bpy) 3 2+ -functionalized microbeads, showing promise in ECL-based bioassay and microscopy. The electrochemiluminescence (ECL) of ruthenium(II) tris(2,2′-bipyridyl) (Ru(bpy) 3 2+ ) with tri- n -propylamine (TPrA) as the good coreactant can be unexpectedly enhanced by a weak coreactant, such as triethanolamine (TEOA). First, the intensity of ECL emitted by Ru(bpy) 3 2+ /TPrA can be remarkably amplified by 10.8-fold after adding some amount of TEOA. Moreover, the ECL layer thickness, measured by self-interference spectroscopy, is also doubled. The enhancement far exceeding the superposition of respective contribution of TPrA and TEOA was elucidated by a “chemical oxidation mechanism”, in which TEOA + ⋅ acts as a chemical enhancer to oxidize TPrA in solution and to accelerate significantly the ECL reaction kinetics. This mechanism was proved by single-photon counting experiment and finite element simulations. In addition, the dual-coreactants strategy works well not only in solution with freely diffusive Ru(bpy) 3 2+ , but also on Ru(bpy) 3 2+ -functionalized microbeads, suggesting that ethanolamines could act as cheap, easily available and low-background enhancers for ECL-based bioanalysis and microscopy.
研究领域:
分析化学
·
生物分析
·
显微技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Sheng-Han Tang,Ying Zhang,Ling Qin,Wei-Guo Yang,Wen-Bin Liang,Jun Yang,Yan Li,Shan-Shan Hu
Despite the significant advantages of electrochemiluminescence (ECL) technology in ultrasensitive analysis of various molecules, the traditional three-electrode ECL system utilizes solution phase as the reaction medium often requires laborious preparations of the solution, leading to a relative complex and potential contamination. In this study, an organic ECL hydrogel based on poly(vinyl alcohol)/sodium alginate/glycerol (PVA/SA/Gly) hydrogel with luminophore tris (2,2′-bipyridine) ruthenium (Ⅱ) hexafluorophosphate ( R u ( b p y ) 3 2 + ) and co-reactant N, N’-bis (salicylidene)-1, 2-propanediamine (PropH) was explored for the construction of a novel solid-phase portable ECL sensing platform to detect heavy metal ions. To be specific, the unique Schiff base cavity structure of PropH specifically coordinated with Co 2+ , altering that its own nitrogen atoms’ electron cloud distributes to hinder co-reaction and reduce the hydrogel’s ECL emission. Notably, this novel ECL system utilized an organic ECL hydrogel as the reaction vessel, streamlining detection procedures and reducing detection time. Furthermore, the organic ECL hydrogel exhibited excellent stability and prolonged storage capability while maintaining strong ECL emission even after extended periods at room temperature. The proposed solid-phase portable ECL sensing platform demonstrates an excellent linear range from 1 nM to 10 mM and low detection limit of 0.31 nM for Co 2+ , providing new strategies for practical application capabilities in the field of food safety detection and environmental monitoring.
研究领域:
分析化学
·
环境科学
·
食品安全
✓ 已验证
产品关联
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2024
·
IF 10.7
Xu Liang,Chang Liu,Yuecong Hu,Yutong Hua,Junjie Liu,Jintao Deng,Zhiwei Zhu,Yuanhua Shao
Multi-signal-based self-calibrating biosensors have become a research focus due to their superior accuracy and sensitivity in recent years. Herein, the potential-resolved differential ECL immunoassay based on dual co-reactants regulation was developed. Meso-tetra(4-carboxyphenyl)porphyrin (TCPP) functionalized zirconium dioxide (ZrO 2 ) composites (TCPP-ZrO 2 ) was first synthesized using TCPP as the luminophore and ZrO 2 as the enhancer and stabilizer. The organic-inorganic hybrid structure of TCPP-ZrO 2 limits the intramolecular motion of TCPP and accelerates the production of reactive radicals of co-reactants, thus exhibiting excellent ECL intensity and stability. Then, the potential-resolved ECL properties of TCPP-ZrO 2 regulated only by two co-reactants were explored. On this basis, a differential ECL immunosensor was constructed for the sensitive and accurate determination of heart-fatty acid binding protein (hFABP) using 2-(dibutylamino)ethanol modified gold nanoparticles (DBAE@Au) as labels (ECL-1, +1.3 V) and ECL-2 (−1.3 V) triggered by potassium persulfate (K 2 S 2 O 8 ) as the internal reference. In the presence of hFABP, DBAE@Au was captured by the sensing interface to generate ECL-1 and consume K 2 S 2 O 8 , leading to a significant decrease of ECL-2. According to the intensity difference between ECL-2 and ECL-1, the detection of hFABP was achieved with a low detection limit and a wide linear range. The proposed differential ECL immunosensor has been applied in testing human serum samples with satisfactory results, demonstrating its promising applications for clinical diagnosis.
研究领域:
生物医学工程
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2015
·
IF 4.513
Zhenyu Zhou,Weiliang Guo,Linru Xu,Qian Yang,Bin Su
In this paper, we report significantly enhanced electrochemiluminescence (ECL) of tris(2,2′-bipyridyl)ruthenium(II) ( Ru ( bpy ) 3 2 + ) at indium tin oxide (ITO) electrodes modified with a thin film consisting of vertically aligned silica mesochannels (SMCs). The perpendicular SMCs allow for the effective mass transport of Ru ( bpy ) 3 2 + and its co-reactants from the solution to the underlying ITO electrode surface. Moreover, due to the ultrasmall diameter (namely 2–3 nm) and the negatively charged surface, SMCs exerted a strong electrostatic attraction to the positively charged Ru ( bpy ) 3 2 + to accelerate its mass transport, resulting in a remarkably increased ECL signal. As a model system, when using tri- n -propylamine (TPrA) as the co-reactant, the intensity of ECL generated at the SMCs-modified ITO electrode was stronger than that at a bare ITO electrode by more than two orders of magnitude (i.e., ∼107-fold increase). In this case, the amount of Ru ( bpy ) 3 2 + required for ECL-based analysis can be considerably reduced. A very low concentration of Ru ( bpy ) 3 2 + , namely 9 μM, was demonstrated to be enough for achieving the sensitive detection of TPrA, nicotine and atropine. Both photomultiplier (PMT) and charge coupled device (CCD) were used to measure the luminescence from the ECL reaction. The obtained detection limits were 0.17 nM for TPrA on the basis of the ECL intensity measurement, 72 nM for nicotine and 38 nM for atropine based on the gray value analysis of captured ECL images.
研究领域:
分析化学
·
电化学
·
生物传感器
·
纳米材料
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分子探针
·
药物检测
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Xuwen Gao,Xiaoxuan Ren,Yaojia Ai,Mengwei Li,Bin Zhang,Guizheng Zou
Coreactant-free electrochemiluminescence (ECL) is promising for removing the exogenous effects of coreactant and simplify the operation procedures and setups of commercialized ECL bioassays. Herein, an electrosterically involved strategy for achieving a low-triggering-potential (+0.21 V vs Ag/AgCl) and coreactant-free ECL from dual-stabilizer-capped CdTe nanocrystals (NCs) is proposed with mercaptopropionic acid (MPA) and hexametaphosphate (HMP) as the capping agents of luminophores. Upon employing the CdTe NCs as the ECL tag for the immunoassay, all the tags in the bioconjugates of the CdTe NCs and the secondary antibody (Ab2|CdTe) as well as in the final achieved sandwich-type immunocomplexes can exhibit efficient coreactant-free ECL with an electrosterically involved triggering potential nature. The bioconjugates of Ab2|CdTe with Ab2 no more than 30 kDa, such as the thyroid stimulating hormone (30 kDa) and the recombinant pro-gastrin releasing peptide (ProGRP, 14 kDa), merely exhibit coreactant-free ECL around +0.24 V, while bioconjugates of Ab2|CdTe with an Ab2 beyond 30 kDa only give off coreactant-free ECL around +0.82 V. Due to the further enhanced electrosteric effect in sandwich-type immunocomplexes, only the ECL immunosensor with ProGRP as the target can give off coreactant-free ECL around +0.24 V. The electrosterically involved and coreactant-free ECL of CdTe NCs is consequently utilized to sensitively and selectively determine the molecular protein ProGRP, which demonstrates a wide linearity range from 0.1 to 2000 pg/mL and a low limit of detection at 0.05 pg/mL (S/N = 3). This low-triggering-potential and coreactant-free combined ECL platform indicates that engineering the surface of CdTe NCs with a protein can improve the performance of ECL tags in a protein-weight-involved electrosterical way.
研究领域:
分析化学
·
生物化学
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纳米技术
·
生物医学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Qianxi Duan,Jingke Pan,Yunxiu Jia,Yizhuo Fu,Beibei Wang,Chao Lu
Understanding the disassembly process is significant for constructing well-defined and multifunctional supramolecular structures. Nowadays, fluorescent microscopy is used as a common technique to observe a real-time disassembly process. However, the addition of the fluorescent probes into nonfluorescent systems might influence the disassembly due to the weak supramolecular interactions. Therefore, it is urgent to explore a novel strategy to monitor supramolecular disassembly in the absence of fluorophores. In this work, we developed a simple and rapid strategy to understand the supramolecular disassembly process based on confined microenvironment-regulated electrochemiluminescence (ECL). It is disclosed that the addition of competitive guests could disassemble the supramolecular architectures and destroy the confined spaces, decreasing the ECL intensity as a result of the reduction of the chemical transformation efficiency of the coreactant. This developed nanoconfinement-tuned ECL strategy offers a simple method to monitor supramolecular disassembly in real time without the addition of any fluorescent dye. It is anticipated that the proposed strategy could be beneficial for a deep understanding of supramolecular disassembly, promoting the design and preparation of new-generation supramolecular assemblies with advanced functions.
研究领域:
超分子化学
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分析化学
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纳米技术
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电化学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
TALANTA
·
2025
·
IF 6.1
Xuemei Pi,Rong Wang,Yiyu Guo,Xianwen Kan
l -Cysteine (L-Cys), one kind of aminothiol, ubiquitously exists in proteins and plays important roles in the food industry and biological processes. Herein, an electrochemiluminescence (ECL) sensor was constructed for the sensitive detection of L-Cys. As an ECL emitter, europium-porphyrin coordination polymer (Eu-PCP) was synthesized and subsequently modified with copper benzene-1,4-dicarboxylate (CuBDC) as a coreaction accelerator, which achieved high self-enhanced ECL responses. CuBDC on Eu-PCP was decomposed by the introduced L-Cys due to the coordination between Cu 2+ and L-Cys, resulting in the decrease of ECL signal. Besides, L-Cys could consume the co-reactant of K 2 S 2 O 8 to reduce the production of free radical SO 4 •– , further decrease ECL signal. Thus, this L-Cys coordination-driven decomposition of coreaction accelerator and consumption of co-reactant strategy was employed to doubly suppress the ECL signal for detecting L-Cys without additional recognition and amplification elements. The constructed ECL sensor exhibited a linear range of 1.0 × 10 −10 –5.0 × 10 −5 mol/L with a limit of detection of 2.0 × 10 −11 mol/L. This work has opened up new prospects for developing novel ECL sensing strategies in food safety monitoring and disease diagnosis.
研究领域:
分析化学
·
生物医学
·
食品安全检测
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2024
·
IF 8
Luting Yan,Yuhao Zhang,Qi Guo,Lei Tian,Falan Li,Qingqing Yang,Yanyan Zhang
Most electrochemiluminescent (ECL) biosensors currently necessitate coreactants that affect sensor detection accuracy and increase sensor construction complexity. This study detailed the creation of an ECL biosensor without coreactant, utilizing Methionine-capped Au nanoclusters (Met-AuNCs) as luminophore and a Pb 2+ -dependent DNAzyme-mediated DNA walker signal amplification strategy to detect Vibrio parahaemolyticus (Vp) in scallops. Double-stranded DNA (dsDNA) was produced through hybridization of the Vp aptamer with DNA walker. The electrode surface was loaded with cDNA labeled with Met-AuNCs. Upon the presence of Vp, it competitively bound to the aptamer, resulting in dsDNA dissociation and exposing the DNAzyme sequence in the DNA walker. The introduction of Pb 2+ initiated the activation and circulation of the DNAzyme-mediated DNA walker on the electrode surface, causing the cDNA to be sheared. As a result, the cDNA-Met-AuNCs on the surface of the electrode decreased, resulting in a reduction of ECL signal intensity. The ECL biosensor detected Vp at a range of 1.00×10 1 -1.00×10 7 CFU/mL, under optimal conditions, with the limit of detection (LOD) being 1.23 CFU/mL. In this work, the biosensor demonstrated excellent stability and specificity and had proven successful in detecting actual samples of aquatic products. This study offers a novel ECL method without a coreactant for the detection of foodborne pathogens in aquatic products.
研究领域:
生物化学
·
纳米技术
·
食品安全
·
分析化学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Zirui Wang,Jilong Zheng,Min Li,Shujia Wang,Zhida Gao,Yan-Yan Song,Chenxi Zhao
Rapid and accurate detection of trace microbial pathogens is essential for human health, environmental monitoring, and food safety. Here, we report the development of a solid-state electrochemiluminescence (ECL) bacterial sensor based on a β-glycosidase (β-Gal)-triggered coreactant generation strategy. Leveraging a three-dimensional TiO2 nanotube array integrated with peroxidase-like MIL-53(Fe), glucose oxidase-like Au nanoparticles, and luminol, this sensor efficiently generates coreactants on-site, enabling sensitive glucose detection. As glucose is the primary product of β-Gal-catalyzed lactose hydrolysis, its concentration directly correlates with β-Gal enzymatic activity, thus allowing for the quantification of β-Gal via ECL signal intensity. Using enterohemorrhagic Escherichia coli (EHEC) as a model of food-borne pathogens, the sensor demonstrates rapid detection (30 min) with an ultralow LOD (0.8 CFU mL–1), representing the most sensitive label-free bacterial ECL sensor reported to date. Furthermore, the ECL electrode was applied for the detection of EHEC on raw salmon slices, underscoring its potential for food safety monitoring.
研究领域:
分析化学
·
生物传感器
·
食品安全
·
纳米技术
·
微生物学
·
电化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemSusChem
·
2021
·Vol. 15
·期 3
·e202102538
·
IF 8.928
Yuen Wai Lui,Bun Chan,Matthew Y. Lui
Graphical Circular Economy : A sustainable methylation process is developed using only inexpensive methanol and bio-based chemicals with a very easy workup and purification procedure. Dimethyl sulfide, a major byproduct of the Kraft pulping process, was used as an inexpensive and sustainable catalyst/co-reagent (methyl donor) for various methylations with dimethyl carbonate (as both reagent and solvent), which afforded excellent yields of O -methylated phenols and benzoic acids, and mono- C -methylated arylacetonitriles. Furthermore, these products could be isolated using a remarkably straightforward workup and purification procedure, realized by dimethyl sulfide‘s neutral and distillable nature and the absence of residual salts. The likely mechanisms of these methylations were elucidated using experimental and theoretical methods, which revealed that the key step involves the generation of a highly reactive trimethylsulfonium methylcarbonate intermediate. The phenol methylation process represents a rare example of a Williamson-type reaction that occurs without the addition of a Brønsted base.
研究领域:
有机化学
·
绿色化学
·
可持续发展
·
催化
·
化学工程
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2020
·
IF 3.807
Fang Hou,Xue-Bo Hu,Shu-Hui Ma,Jun-Tao Cao,Yan-Ming Liu
A sandwich-type ECL platform was fabricated for sensitive detection of prostate specific antigen (PSA) by using AuNPs decorated MoS 2 (MoS 2 -AuNPs) as electrode material and SiO 2 nanoparticles labeled with secondary antibody (Ab 2 ) and glucose oxidase (Ab 2 -SiO 2 -GOD) as signal probes. Owing to the excellent conductivity and large surface area of MoS 2 -AuNPs, the fast electron transfer process and the immobilization of numerous biomolecules can be achieved. The GOD labeled signal probes can realize the in situ generation of H 2 O 2 as coreactant to amplify the ECL intensity of luminol. The developed immunosensor displayed a wide linear range of 0.5 pg mL − 1 –10.0 ng mL − 1 and low detection limit of 0.20 pg mL − 1 for PSA detection. The immunosensor also has been successfully applied for the analysis of PSA in human serum samples. Furthermore, the proposed strategy shows great application prospects for determination of other biomarkers in biological samples.
研究领域:
分析化学
·
生物传感器
·
生物医学工程
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Han Zhang,Qiyi Lu,Fumei Zuo,Ruo Yuan,Shihong Chen
A novel “signal-on” biosensor for the detection of concanavalin A (Con A) was designed utilizing an enzymatic reaction to in situ generate coreactant of poly(9,9-di- n -octylfluorenyl-2,7-diyl) (PFO). Firstly, PFO polymers were wrapped with graphene oxide nanosheets to obtain GO@PFO nanocomposite, which was modified onto the surface of the electrode as a matrix for immobilizing phenoxy-derivatized dextran (Dexp) through π-π stacking interaction. Then Con A interacted with Dexp through the specific carbohydrate-protein interaction. Finally, glucose oxidase (GOx) and lactate oxidase (LOx) decorated gold nanoparticles@C 60 -Polyamidoamine (Au@C 60 -PAMAM) was bound onto Con A via a biospecific interaction between Con A and the internal residues of GOx, thus achieving a sandwich structure. In this construction strategy, LOx catalyzed the oxidation of lactate to in situ generate H 2 O 2 , which could act as the coreactant of PFO to enhance the ECL intensity. Due to the excellent ECL property of PFO–H 2 O 2 system, our prepared biosensor showed a wide linear range from 0.001 ng/mL to 10 ng/mL, and a low detection limit down to 0.0003 ng/mL. The integration of excellent ECL property of PFO–H 2 O 2 system and excellent catalytic activity of Au@C 60 -PAMAM would open a new and promising platform for fabricating ECL biosensors and biocatalysts.
研究领域:
分析化学
·
生物传感器
·
电化学
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
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