ANALYST
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2022
·Vol. 147
·期 24
·5687-5693
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IF 5.227
Yuyuan Zhang,Jing Lei,Wei Liu,Yan Jin,Baoxin Li
Various CRISPR/Cas12a-based biosensing systems have been developed in the past few years, and most of these systems used Taqman probes to report signals through fluorescence resonance energy transfer (FRET). In this study, we explored chemiluminescence resonance energy transfer (CRET) as the readout mode for CRISPR/Cas12a-based biosensing. The chemiluminescence (CL) reaction of bis(2,4,6-trichlorophenyl) oxalate (TCPO) and H2O2 was used to excite the fluorophore dye of the Taqman probe. Different from FRET, CRET does not need external excitation light, which can effectively avoid autofluorescence and photobleaching. The detection limit of this CRET readout mode was estimated to be 10 pM for target DNA, which was about 8 times lower than that of the widely used FRET readout mode. These results suggest that CRET can serve as a rapid, sensitive and simple readout mode of CRISPR/Cas12-based biosensing, and can further enrich the toolbox of CRISPR/Cas12-based biosensing.
研究领域:
分子生物学
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生物化学
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诊断学
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DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
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2020
·
IF 3.594
Wanying Fu,Hongying Wang,Yanwei Chen,Jinglei Ding,Guiye Shan
In this work, a gold nanocluster with lysine (Lys) as a ligand was fabricated, and a novel nanocomposite probe (AuNCs@Lys-MnO 2 ) was prepared based on fluorescence resonance energy transfer (FRET). AuNCs@Lys-MnO 2 was manufactured for sensitive and rapid sensing of glutathione (GSH) in human serum. Gold nanoclusters (AuNCs@Lys) and manganese dioxide nanosheets (MnO 2 nanosheets) are combined by electrostatic interaction. MnO 2 nanosheets can quickly quench the fluorescence of AuNCs@Lys through FRET. GSH has strong reducing ability, which can reduce MnO 2 and degrade AuNCs@Lys-MnO 2 FRET system. The presence of GSH subsequently releases AuNCs@Lys, which causes partial recovery of fluorescent signal. The nanocomposite probe is successfully applied to detect GSH in human serum. Detection range is near the normal value in human serum, and there is no need to dilute the serum during the detection process. This is a fast, simple, and cost-effective strategy. The method can detect GSH in the concentration range of 0.5 to 1000 μM, and has a very high sensitivity, the detection limit is as low as 5 nM.
研究领域:
分析化学
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纳米技术
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生物传感器
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生物医学工程
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DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2018
·
IF 5.667
Cheng-Yu Li,Ya-Feng Kang,Bei Zheng,Chun-Miao Xu,Chong-Yang Song,Dai-Wen Pang,Hong-Wu Tang
We report a new upconversion nanoparticles (UCNPs) based luminescent resonance energy transfer (LRET) analytical platform by making use of optical tweezers technology. The LRET model is designed by simultaneously conjugating Yb 3+ and Er 3+ co-doped UCNPs (as the donors) and tetramethyl rhodamine (TAMRA) molecules (as the acceptors) on microspheres to fabricate complex microspheres. Upon a single complex microsphere entering the three-dimensional potential well formed with a tightly focused 980 nm Guassian-shaped laser beam, it is optically trapped and concurrently the upconversion emission is excited, whereby the donor signals are transferred to the acceptors. As a proof-of-concept investigation, microRNA-21 sequences are selected as the targets, by which the distance between the two perfectly matched luminophors is controlled to several nanometers via nucleic acid hybridization. Without the involvement of luminescence amplification strategies, the proposed single microsphere based LRET method shows highly competitive sensitivity with a limit of detection down to 114 fM and satisfactory specificity towards microRNAs detection. Moreover, its practical working ability is demonstrated by credibly quantifying the absolute contents of miRNA-21 sequences in three cancer cell lines and even tracing the targets in as few as 100 cancer cells. Thus, this favorable analytical methodology provides an alternative for bioassays and holds certain potential in biomedical applications.
研究领域:
生物物理
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分析化学
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生物医学工程
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分子生物学
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产品关联
DOI
高影响因子
Analytical Methods
·
2016
·Vol. 8
·期 48
·8506-8513
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IF 1.915
Zhen Sheng,Jianfeng Zhang,Chenyu Li,Ligang Chen
The development of new, sensitive and simple methods for the rapid detection of dimethoate (DMT) in water is vitally important. A fluorescent probe technique has been developed for the rapid trace detection of pesticide residues. In our study, the method used is based on the fluorescence resonance energy transfer, which is also known as FRET. Dithizone (DZ) was bound to cadmium (Cd) on the surface of CdTe QDs to form a DZ–Cd complex. Then, the fluorescence of the CdTe QDs was immediately quenched because of the FRET between the CdTe QDs and the surface of DZ–Cd complex. After the addition of DMT to the DZ-functionalized QDs, DZ was separated from the surface of the QDs and was replaced by DMT, and the fluorescence of the QDs was restored. This method was validated using water samples spiked with DMT. Two good linear relationships were obtained in the range from 0.01 to 5.0 μg mL−1 and 5.0 to 40 μg mL−1; the detection limit was calculated to be 0.005 μg mL−1, and reasonable recoveries (88.6–95.3%) were achieved for the direct detection of DMT in water samples. This method is rapid, convenient and environmentally acceptable and shows potential for the rapid detection of DTM in water samples.
研究领域:
环境科学
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分析化学
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产品关联
DOI
高影响因子
JOURNAL OF LUMINESCENCE
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2013
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IF 2.144
Huilin Tao,Xiufen Liao,Mingze Xu,Shuhuai Li,Fuxin Zhong,Zhongsheng Yi
The fluorescence resonance energy transfer between fluorescent brightener (FB, donor) and CdTe quantum dots (CdTe QDs, acceptor) was studied and a new method for detecting Hg 2+ ions in water samples was accordingly developed in this paper. It was found that the effective energy transfer occurred from FB to CdTe QDs in Tris–HCl buffer solution at pH=8.2, in which the QDs' fluorescence intensity increased greatly. Then the fluorescence quenching occurred as Hg 2+ interacted with CdTe. Under the optimized conditions, there was a linear relationship between the fluorescence quenching intensity and the concentration of Hg 2+ . Hg 2+ could be determined in the concentration range of 8.0×10 −9 –8.0×10 −7 g L −1 with a detection limit of 6.6×10 −10 g L −1 ( S / N =3). The method was applied to the determination of Hg 2+ in water samples via the standard addition method, and the recovery rate obtained was 97.3–105.5%.
研究领域:
分析化学
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环境科学
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DOI
高影响因子
JOURNAL OF ALLOYS AND COMPOUNDS
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2017
·
IF 3.133
Yan Gao,Yao Cheng,Feng Huang,Hang Lin,Ju Xu,Yuansheng Wang
In this work, a new type of luminescent temperature sensor based on the Sn 2+ →Mn 2+ resonance energy transfer is designed, which shows great potential in high temperature optical thermometry. Using the micro-sized Sn 2+ /Mn 2+ :α-Sr 2 P 2 O 7 phosphor fabricated by carbon reduction method as optical thermometer, the sensitivity in the temperature range of 423 K–623 K is well superior to that for the other micro/nano luminescent temperature sensing materials reported previously, and the maximal absolute sensitivity reaches as high as 0.027 K −1 at 623 K. Analyses of the temperature-dependent photoluminescence spectra and configurational coordinate diagrams for Sn 2+ :α-Sr 2 P 2 O 7 and Mn 2+ :α-Sr 2 P 2 O 7 indicate that the temperature-sensitive variation in fluorescence intensity ratio of Sn 2+ to Mn 2+ is originated from the difference in thermal quenching activation energy for 3 P J (J = 0,1) levels of Sn 2+ (4053 cm −1 ) and 4 T 1 level of Mn 2+ (2464 cm −1 ). These results may provide useful inspiration for building highly sensitive self-referencing optical thermometers based on energy transfer induced sensitized luminescence.
研究领域:
材料科学
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光学工程
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DOI
高影响因子
JOURNAL OF FLUORESCENCE
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2023
·
IF 2.7
Qiu Lei,Gao Mingcong,Li Jiawei,Xu Guanhong,Wei Fangdi,Yang Jing,Hu Qin,Cen Yao
Sensitive and convenient strategy of tyrosinase (TYR) and its inhibitor atrazine is in pressing demand for essential research as well as pragmatic application. In this work, an exquisite label-free fluorometric assay with high sensitivity, convenience and efficiency was described for detecting TYR and the herbicide atrazine on the basis of fluorescent nitrogen-doped carbon dots (CDs). The CDs were prepared via one-pot hydrothermal reaction starting from citric acid and diethylenetriamine. TYR catalyzed the oxidation of dopamine to dopaquinone derivative which could quench the fluorescence of CDs through a fluorescence resonance energy transfer (FRET) process. Thus, a sensitive and selective quantitative evaluation of TYR can be constructed on the basis of the relationship between the fluorescence of CDs and TYR activity. Atrazine, a typical inhibitor of TYR, inhibited the catalytic activity of TYR, leading to the reduced dopaquinone and the fluorescence was retained. The strategy covered a broad linear range of 0.1–150 U/mL and 4.0–80.0 nM for TYR and atrazine respectively with a low detection limit of 0.02 U/mL and 2.4 nM/mL. It is also demonstrated that the assay can be applied to detect TYR and atrazine in spiked complex real samples, which provides infinite potential in application of disease monitoring along with environmental analysis.
研究领域:
生物化学
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环境科学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2017
·
IF 7.78
Han Zhang,Fumei Zuo,Xingrong Tan,Shenghao Xu,Ruo Yuan,Shihong Chen
An electrochemiluminescencent (ECL) biosensor was designed for the determination of insulin using a novel ECL resonance energy transfer (ECL-RET) strategy. In this strategy, carboxyl poly(9,9-dioctyfluorenyl-2,7-diyl) dots (PFO dots) were worked as ECL donor and 3,4,9,10-perylenetetracar-boxylic acid (PTCA) exploited as ECL acceptor, and hydrogen peroxide (H 2 O 2 ) employed as the coreactant. The ECL donor and ECL acceptor were separately labeled with primary antibody (Ab 1 ) and secondary antibody (Ab 2 ), forming a sensing interface to the analyte target , insulin. In this expected sandwich-type ECL biosensor, PFO dots acted as sensing platform and PTCA employed as labels to quench the ECL emission of PFO dots. During the determination process, ECL signal of PFO dots was decreased in a gradual way by the increase of insulin concentration, and the quenching mechanism was also investigated. Under the optimal experimental conditions, the constructed biosensor exhibited an excellent performance, including a wide linear range from 1.0 × 10 −5 ng/mL to 1.0 × 10 2 ng/mL, low detection limit of 3.0 × 10 −6 ng/mL, good stability and selectivity for the detection of insulin. This pair of PFO-PTCA, as a new donor-acceptor pair in ECL-RET system, would provide a promising platform for bioanalysis in ECL field.
研究领域:
生物传感
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分析化学
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DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
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2015
·
IF 5.636
Yan-Ran Li,Qian Liu,Zhangyong Hong,He-Fang Wang
For the widely used “off-on” fluorescence (or phosphorescence) resonance energy transfer (FRET or PRET) system, the separation of donors and acceptors species was vital for enhancing the sensitivity. To date, separation of free donors from FRET/PRET inhibition systems was somewhat not convenient, whereas separation of the target-induced far-between acceptors has hardly been reported yet. We presented here a novel magnetic separation-assistant fluorescence resonance energy transfer (MS-FRET) inhibition strategy for highly sensitive detection of nucleolin using Cy5.5-AS1411 as the donor and Fe3O4-polypyrrole core–shell (Fe3O4@PPY) nanoparticles as the NIR quenching acceptor. Due to hydrophobic interaction and π–π stacking of AS1411 and PPY, Cy5.5-AS1411 was bound onto the surface of Fe3O4@PPY, resulting in 90% of fluorescence quenching of Cy5.5-AS1411. Owing to the much stronger specific interaction of AS1411 and nucleolin, the presence of nucleolin could take Cy5.5-AS1411 apart from Fe3O4@PPY and restore the fluorescence of Cy5.5-AS1411. The superparamagnetism of Fe3O4@PPY enabled all separations and fluorescence measurements complete in the same quartz cell, and thus allowed the convenient but accurate comparison of the sensitivity and fluorescence recovery in the cases of separation or nonseparation. Compared to nonseparation FRET inhibition, the separation of free Cy5.5-AS1411 from Cy5.5-AS1411-Fe3O4@PPY solution (the first magnetic separation, MS-1) had as high as 25-fold enhancement of the sensitivity, whereas further separation of the nucleolin-inducing far-between Fe3O4@PPY from the FRET inhibition solution (the second magnetic separation, MS-2) could further enhance the sensitivity to 35-fold. Finally, the MS-FRET inhibition assay displayed the linear range of 0.625–27.5 μg L–1 (8.1–359 pM) and detection limit of 0.04 μg L–1 (0.05 pM) of nucleolin. The fluorescence intensity recovery (the percentage ratio of the final restoring fluorescence intensity to the quenched fluorescence intensity of Cy5.5-AS1411 solution by 0.09 g L–1 Fe3O4@PPY) was enhanced from 36% (for nonseparation) to 56% (for two magnetic separations). This is the first accurate evaluation for the effect of separating donor/acceptor species on the FRET inhibition assay.
研究领域:
生物化学
·
分析化学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2020
·
IF 10.257
Xixia Liu,Yaoyao Hou,Sirui Chen,Juewen Liu
Dopamine is one of the most important neurotransmitters . A high-quality DNA aptamer for dopamine was reported in 2018. However, fundamental understanding of its binding and folding is lacking, which is critical for related biosensor design. Herein, we performed careful assays using a label-free technique called isothermal titration calorimetry (ITC) to study its secondary structure . We divided this aptamer into four regions and individually examined each of them. We confirmed two stems, but the third stem is believed to be part of a loop. The aptamer was then truncated. The original aptamer had a K d of 2.2 ± 0.3 μM at 25 °C. Shortening the structure by one or two base pairs increased the K d to 6.9 and 44.4 μM, respectively. Dopamine binding was promoted by both increasing the Mg 2+ concentration and decreasing the temperature. At 5 °C, a K d of 0.4 μM was achieved. Based on this understanding, we designed two fluorescence resonance energy transfer (FRET) quenching biosensors that differ only by a base pair. The shorter sensor had 3-fold higher sensitivity and a detection limit of 0.9 μM. In 1% fetal bovine serum, the sensor retained a similar limit of detection of 1.14 μM. A two-fluorophore ratiometric FRET sensor was also demonstrated with a low detection limit of 0.12 μM. This work indicated the feasibility of designing folding-based sensors for dopamine, and this design can be extended to other sensing modalities such as electrochemistry and colorimetry .
研究领域:
分析化学
·
生物化学
·
生物物理
·
神经科学
·
分子生物学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2016
·
IF 7.476
Xiaomeng Zhang,Shushu Ding,Sumei Cao,Anwei Zhu,Guoyue Shi
Selective and sensitive detection of extracellular lactate is of fundamental significance for studying the metabolic alterations in tumor progression. Here we report the rational design and synthesis of a quantum-dot-hydrogel-based fluorescent probe for biosensing and bioimaging the extracellular lactate. By surface engineering the destabilized quantum dot sol with Nile Blue , the destabilized Nile-Blue-functionalized quantum dot sol cannot only self-assemble forming quantum dot hydrogel but also monitor lactate in the presence of nicotinamide adenine dinucleotide cofactor and lactate dehydrogenase through fluorescence resonance energy transfer . Notably, the surface engineered quantum dot hydrogel show high selectivity toward lactate over common metal ions , amino acids and other small molecules that widely coexist in biological system. Moreover, the destabilized Nile-Blue-functionalized quantum dots can encapsulate isolated cancer cells when self-assembled into a hydrogel and thus specifically detect and image the extracellular lactate metabolism. By virtue of these properties, the functionalized quantum dot hydrogel was further successfully applied to monitor the effect of metabolic agents.
研究领域:
生物传感
·
生物成像
·
医学检测
✓ 已验证
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2025
·
IF 9.8
Yujia Song,Li Tian,Ruidan Li,Yanjia Guo,Pengfei Han,Guangping Ma,Hanyue Jiang,Wenzhuo Wang,Juan Lu
In this work, g-C 3 N 4 of N-rich vacancy (CNNV) and mesoporous SiO 2 were synthesized. A novel electrochemiluminescence (ECL) sensor for curcumin (Cur) based on CNNV@SiO 2 and Ti 2 AlC was constructed. An initial ECL signal was generated from the CNNV-S 2 O 8 2 − system. SiO 2 significantly adsorbed more S 2 O 8 2 − , thereby enhancing the ECL signal. Ti 2 AlC served as an effective co-reaction promoter, further amplifying the ECL signal of CNNV@SiO 2 -S 2 O 8 2 − system. With the addition of Cur, the ECL signal also decreased, which can be attributed to the resonance energy transfer (RET) mechanism between CNNV (donor) and Cur (acceptor). Under optimal conditions, a strong linear relationship was observed between the change in ECL signal (ΔI) and the logarithm of Cur concentration (lgC Cur ), spanning from 5 × 10 −15 to 5 × 10 −9 mol L −1 with a detection limit of 1.7 × 10 −15 mol L −1 . The constructed ECL sensor was successfully applied to detect Cur in real samples.
研究领域:
分析化学
·
材料科学
·
生物医学
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产品关联
DOI
PubMed
高影响因子
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